Heterocyclic compounds as modulators of stimulator of interferon genes (sting)

ABSTRACT

The present invention relates to compounds of formula (I) and salts, stereoisomers, tautomers or N-oxides thereof that are useful as modulators of STING (Stimulator of Interferon Genes). The present invention further relates to the compounds of formula (I) for use as a medicament and to a pharmaceutical composition comprising said compounds.

FIELD OF THE INVENTION

The present invention relates to compounds of formula (I) and salts, stereoisomers, tautomers or N-oxides thereof that are useful as modulators of STING (Stimulator of Interferon Genes). The present invention further relates to the compounds of formula (I) for use as a medicament and to a pharmaceutical composition comprising said compounds.

BACKGROUND OF THE INVENTION

The cellular innate immune system is essential for recognizing pathogen infection and for establishing effective host defense. The adaptor protein STING (Stimulator of Interferon Genes), also known as TMEM 173, MPYS, MITA and ERIS, has been identified as a central signaling molecule in the innate immune response to cytosolic nucleic acids (H. Ishikawa, G. N. Barber, Nature, 2008, vol. 455, pp. 674-678). STING inter alia induces type I interferon (IFN) production when cells are infected with intracellular pathogens, such as viruses, mycobacteria and intracellular parasites.

Activation of STING promotes IRF3 and NFkB-dependent signaling leading in consequence to production of proinflammatory cytokines and interferons, including type I and type III interferons and TNF α of particular importance in cancer immunotherapy. STING is responsible for sensing of cytoplasmic nucleic acids and their derivatives called cyclic dinucleotides (CDN), both of pathogen or host origin (e.g. double stranded DNA from bacteria or viruses and cytoplasmic self-DNA).

Endogenous STING direct agonist 2′,3′-cGAMP (2′,3′-cyclic guanosine monophosphate-adenosine monophosphate) is produced in mammalian cells by enzyme cGAS (cyclic GMP-AMP synthase, MB21D1 or C6orfl50) (P. Gao et al., Cell, 2013, 153, pp. 1094-1107, Wu et al. Science, 2013, 339, pp. 786-791) and has proven activity in modulating STING-dependent pathway, together with its derivatives (L. Corrales et al., J Immunother Cancer, 2013, 1(Suppl 1): O15, L. Corrales et al., Cell Rep., 2015, May 19; 11(7), pp. 1018-30, S-R. Woo et al., Trends Immunol., 2015, 36 (4), 250, J. Fu et al., Sci. Trans. Med., Vol. 7, Issue 283, pp. 283ra52).

Recent evidence supports findings that once STING is activated by CDN within tumor microenvironment, preferably in tumor-resident dendritic cells, it promotes type I IFN and TNF α release which results in immunity-mediated anti-tumor response. STING-dependent activation of antigen-presenting cells (APC) efficiently drives highly specific T-cell priming against neoantigens (L. Corrales and T F. Gajewski, Clin Cancer Res, 2015, 21 (21), pp. 4774-9). STING activation not only provides generation of tumor-specific killer T cells, which directly eradicate tumors, but also results in vaccine-like long-lasting immunity protecting from cancer recurrence.

Thus, synthetic STING agonists are of special interest as potential anticancer agents. The activation or inhibition of type I interferon production is an important strategy for the treatment or prevention of human diseases including viral infections and autoimmune disease. It has been found that compounds activating or inhibiting type I interferon production may be useful not only in infectious disease innate immunity, but also in cancer (L. Zitvogel et al., Nature Reviews Immunology, 2015, vol. 15(7), pp. 405-414), allergic diseases (J. Moisan et al., Am. J. Physiol. Lung Cell Mol. Physiol., 2006, vol. 290, L987-995), neurodegenerative diseases such as amyotrophic lateral sclerosis and multiple sclerosis (H. Lemos et al., J. Immunol, 2014, vol. 192(12), pp. 5571-8; E. Cirulli et al., Science, 2015, vol. 347(6229), pp. 1436-41; A. Freischmidt et al., Nat. Neurosci., vol. 18(5), 631-6), other inflammatory conditions such as irritable bowel disease (S. Rakoff-Nahoum, Cell, 2004, 23, 118(2), pp. 229-41), and as vaccine adjuvants (Persing et al., Trends Microbiol. 2002, 10(10 Suppl), S32-7; Dubensky et al, Therapeutic Advances in Vaccines, published online Sep. 5, 2013).

STING is essential for antimicrobial host defense, including protection against a range of DNA and RNA viruses and bacteria (reviewed in Barber et al., Nat. Rev. Immunol., 2015, vol. 15(2), pp. 87-103, Ma and Damania, Cell Host & Microbe, 2016, vol. 19(2), pp. 150-158). Herpesviridae, Flaviviridae, Coronaviridae, Papillomaviridae, Adenoviridae, Hepadnaviridae, ortho- and paramyxoviridae and rhabdoviridae have evolved mechanisms to inhibit STING mediated Type I interferon production and evade host immune control (Holm et al., Nat Comm., 2016, vol. 7, p. 10680; Ma et al., PNAS2015, vol. 112(31) E4306-E4315; Wu et al., Cell Host Microbe, 2015, vol. 18(3), pp. 333-44; Liu et al., J Virol, 2016, vol. 90(20), pp. 9406-19; Chen et al., Protein Cell 2014, vol. 5(5), pp. 369-81; Lau et al., Science, 2013, vol. 350(6260), pp. 568-71; Ding et al., J Hepatol, 2013, vol. 59(1), pp. 52-8; Nitta et al., Hepatology, 2013, vol. 57(1), pp. 46-58; Sun et al., PloS One, 2012, vol. 7(2), e30802; Aguirre et al., PloS Pathog, 2012, vol. 8(10), e1002934; Ishikawa et al., Nature, 2009, vol. 461(7265), pp. 788-92). Thus, small molecule activation of STING is considered to be beneficial for treatment of these infectious diseases.

In contrast, increased and prolonged type I IFN production is associated with a variety of chronic infections, including Mycobacteria (Collins et al., Cell Host Microbe, 2015, vol. 17(6), pp. 820-8); Wassermann et al., Cell Host Microbe, 2015, vol. 17(6), pp. 799-810; Watson et al., Cell Host Microbe, 2015, vol. 17(6), pp. 811-9), Franciscella (Storek et al., J Immunol., 2015, vol. 194(7), pp. 3236-45; Jin et al., J Immunol., 2011, vol. 187(5), pp. 2595-601), Chlamydia (Prantner et al., J Immunol, 2010, vol. 184(5), pp. 2551-60), Plasmodium (Sharma et al., Immunity, 2011, vol. 35(2), pp. 194-207), and HIV (Herzner et al., Nat Immunol, 2015, vol. 16(10), pp. 1025-33; Gao et al., Science, 2013, vol. 341(6148), pp. 903-6). Similarly, excess type I interferon production is found among patients with complex forms of autoimmune disease. Genetic evidence in humans and support from studies in animal models support the hypothesis that inhibition of STING results in reduced type I interferon that drives autoimmune disease (Y. J. Crow et al., Nat. Genet., 2006, vol. 38(8), pp. 38917-920, D. B. Stetson et al., Cell, 2008, pp. 134587-598). Therefore, inhibitors of STING provide a treatment to patients with chronic type I interferon and proinflammatory cytokine production associated with infections or complex autoimmune diseases. Allergic diseases are associated with a Th2-based immune-response to allergens. Th2 responses are associated with raised levels of IgE, which, via its effects on mast cells, promotes a hypersensitivity to allergens, resulting in the symptoms seen, for example, in allergic rhinitis and asthma. In healthy individuals the immune-response to allergens is more balanced with a mixed Th2/Th1 and regulatory T cell response. Induction of Type 1 interferons have been shown to result in reduction of Th2-type cytokines in the local environment and promote Th1/Treg responses. In this context, induction of type 1 interferons by, for example, activation of STING, may offer benefit in treatment of allergic diseases such as asthma and allergic rhinitis (J. P. Huber et al., J Immunol, 2010, vol. 185, pp. 813-817).

In view of the above, compounds modulating STING are useful for treating one or more diseases selected from the group consisting of inflammatory, allergic, and autoimmune diseases, infectious diseases, cancer, pre-cancerous syndromes, and/or as immunogenic composition or vaccine adjuvants. Of particular relevance is the immunotherapy of cancer and viral infections, in particular prostate cancer, renal carcinoma, melanoma, pancreatic cancer, cervical cancer, ovarian cancer, colon cancer, head and neck cancer, lung cancer, fibrosarcoma and breast cancer. Furthermore, activation of local immune response to the lesions is considered to be preferably a systemic therapeutic approach.

Accordingly, there is a need for compounds modulating the activity of STING, and accordingly, provide a therapeutic impact in the treatment of diseases, in which the modulation of STING is beneficial.

OBJECTS AND SUMMARY OF THE INVENTION

It is therefore an object of the present invention to provide compounds, which modulate STING, in particular, compounds, which act as STING agonists, thereby activating STING. In particular, there is an interest in providing compounds, which have high activity as STING agonists.

It is another object of the present invention to provide compounds, which are suitable for use as a medicament. It is another object of the present invention to provide compounds, which are suitable for use in the treatment of one or more diseases, which are linked to STING modulation. It is yet another object to provide compounds, which are suitable for use in the treatment of one or more diseases selected from the group consisting of inflammatory diseases, allergic diseases, autoimmune diseases, infectious diseases, cancer, and pre-cancerous syndromes. In particular, it is an object to provide compounds, which are suitable for the treatment of cancer, in particular prostate cancer, lung cancer, breast cancer, head and neck cancer, bladder cancer, and/or melanoma. It is yet another object to provide compounds, which are suitable for use in immunogenic compositions and as vaccine adjuvants.

It is another object of the present invention to provide compounds, which are functionalized such that covalent attachment to suitable carriers may be facilitated.

The above objects can be achieved by the compounds of formula (I) as defined herein as well as pharmaceutical compositions comprising the same, and by the medical uses thereof.

The inventors of the present invention inter alia surprisingly found that the compounds of formula (I) as defined herein modulate STING, in particular act as STING agonists. Accordingly, the compounds of formula (I) can be used as a medicament, in particular for the treatment of one or more diseases selected from the group consisting of inflammatory diseases, allergic diseases, autoimmune diseases, infectious diseases, cancer, and pre-cancerous syndromes. In particular, the compounds of formula (I) are suitable for the treatment of cancer, in particular prostate cancer, lung cancer, breast cancer, head and neck cancer, bladder cancer, and/or melanoma. Further, the compounds of formula (I) are suitable for use in immunogenic compositions and as vaccine adjuvants.

In a first aspect, the present invention therefore relates to a compound of formula (I)

or a salt, stereoisomer, tautomer, or N-oxide thereof, wherein

-   X¹ is CH or N; -   X² is CR³ or N; -   R¹, R² and R³ are independently H, OH, NR^(c)R^(D), CN, halogen,     C₁-C₄-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₄-alkoxy, aryloxy,     benzyloxy, C(═O)R^(E), NR^(F)C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 4- to 6-membered saturated, partially or fully unsaturated, or     aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl,     heterocyclyloxy, or heterocyclyl-C₁-C₂-alkyl, or 8- to 10-membered     saturated, partially or fully unsaturated, or aromatic carbobicyclyl     or heterobicyclyl, wherein the aforementioned heterocyclic or     heterobicyclic rings comprise one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R⁴ is a 5- or 6-membered aromatic carbocyclic or heterocyclic ring,     or a 9- or 10-membered aromatic carbobicyclic or heterobicyclic     ring, wherein the heterocyclic or heterobicyclic ring comprises at     least one nitrogen atom and optionally one or more, same or     different additional heteroatoms selected from O, N or S, wherein     said N- and/or S-atoms are independently oxidized or non-oxidized,     and wherein each substitutable carbon or heteroatom in the     aforementioned cyclic rings is independently unsubstituted or     substituted with one or more same or different substituents R^(X); -   R⁵ is a 5- or 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y);     and wherein -   R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₄-alkyl,     NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₂-alkoxy-C₁-C₄-alkyl, or a 3- or     4-membered saturated carbocyclyl or heterocyclyl, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(C) and R^(D) are independently H, or C₁-C₂-alkyl; or -   R^(C) and R^(D) together with the nitrogen atom to which they are     bonded form a 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the heterocyclic ring is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(E) is H, C₁-C₂-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, phenyl, benzyl,     OR^(G), or NR^(H)R^(I); or a 5- or 6-membered saturated, partially     or fully unsaturated heterocyclyl, wherein said heterocyclic ring     comprises one or more, same or different heteroatoms selected from     O, N or S, wherein said N- and/or S-atoms are independently oxidized     or non-oxidized, and wherein each substitutable carbon or heteroatom     in the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(F); -   R^(F) is H, C₁-C₂-alkyl, C₃-C₆-cycloalkyl, phenyl, benzyl, or     C(═O)NR^(H)R^(I); -   R^(G) is H, C₁-C₂-alkyl, or 5- or 6-membered aromatic carbocyclyl,     carbocyclyl-C₁-C₂-alkyl, heterocyclyl, or heterocyclyl-C₁-C₂-alkyl,     wherein the aforementioned heterocyclic rings comprise one or more,     same or different heteroatoms selected from O, N or S, wherein said     N-atoms are independently oxidized or non-oxidized; -   R^(H) and R^(I) are independently H, C₁-C₂-alkyl, or 5- or     6-membered aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl,     heterocyclyl, or heterocyclyl-C₁-C₂-alkyl, wherein the     aforementioned heterocyclic rings comprise one or more, same or     different heteroatoms selected from O, N or S, wherein said N-atoms     are independently oxidized or non-oxidized; or -   R^(H) and R^(I) together with the nitrogen atom to which they are     bonded form a 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the heterocyclic ring is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(X) is OH, NR^(C)R^(D), halogen, CN, NO₂, C₁-C₂-alkyl,     C₁-C₂-haloalkyl, NR^(C)R^(D)—C₁-C₄-alkyl, R^(C)O—C₁-C₄-alkyl,     C₁-C₂-alkoxy, C(═O)R^(E), or two R^(X) form ═O, or two R^(X)     together with the carbon atom to which they are bonded form a 3- to     5-membered saturated, partially or fully unsaturated, or aromatic     carbocyclic ring; -   R^(Y) is halogen, CN, OH, C₁-C₂-alkyl, HO—C₁-C₂-alkyl,     C₃-C₆-cycloalkyl, C₁-C₂-alkoxy, NR^(C)R^(D), S(═O)₂NR^(C)R^(D),     C(═O)R^(E), or 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl,     heterocyclyl, and heterocyclyl-C₁-C₂-alkyl, wherein the     aforementioned heterocyclic rings comprise one or more, same or     different heteroatoms selected from O, N or S, wherein said N-     and/or S-atoms are independently oxidized or non-oxidized, and     wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X); or two R^(Y)     form ═O; or two R^(Y) attached to identical or neighboring carbon     atoms may form a 3-membered carbocyclic ring;     with the proviso that     either     any one of -   R¹, R², or R³ is NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 8- to 10-membered saturated, partially or fully unsaturated, or     aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X);     or     any one of     R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is     NR^(C)R^(D)—C₁-C₄-alkyl;     or -   R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl, or C₁-C₂-alkoxy-C₁-C₄-alkyl;     or     any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a     substituent R^(X), wherein -   R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, or     R^(C)O—C₁-C₄-alkyl.

In one embodiment, the present invention relates to a compound of formula (I) as defined above, with the proviso that

either

-   R² is NHR^(D), NHR^(D)—C₁-C₄-alkyl, NH—(C₁-C₄-alkylene)-NR^(C)R^(D),     NR^(F)—(C₁-C₄-alkylene)-NHR^(D), O—(C₁-C₄-alkylene)-NHR^(D), or 8-     to 10-membered saturated heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises at least one nitrogen atom and     optionally one or more, same or different heteroatoms selected from     O, N or S, wherein said N- and/or S-atoms are independently oxidized     or non-oxidized, and wherein each substitutable carbon or heteroatom     in the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(X);     or -   R² is NR^(F)C(═O)R^(E), wherein R^(E) is NHR^(D)—C₁-C₄-alkyl;     or -   R^(N) is NHR^(D)—C₁-C₄-alkyl;     or -   any one of the substituents R², R⁴, R⁵, R^(N) carries a substituent     R^(X), wherein     R^(X) is OH, NHR^(D), NHR^(D)—C₁-C₄-alkyl, or HO—C₁-C₄-alkyl.

In another embodiment, the present invention relates to a compound of formula (I) as defined above, with the proviso that

either

-   R² is NH₂, NH₂—C₁-C₂-alkyl, NH—(C₁-C₃-alkylene)-NH₂,     NH—(C₁-C₃-alkylene)-NHCH₃, NH—(C₁-C₃-alkylene)-N(CH₃)₂,     O—(C₁-C₃-alkylene)-NH₂, or O—(C₁-C₃-alkylene)-NHCH₃;     or -   R² is NHC(═O)R^(E), wherein R^(E) is NH₂—C₁-C₄-alkyl;     or -   R^(N) is NH₂—C₁-C₃-alkyl;     or -   any one of the substituents R², R⁴, or R⁵ carries a substituent     R^(X), wherein     R^(X) is OH, NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, or HO—C₁-C₂-alkyl.

The above defined provisos focus on the presence of a functionalization, which may facilitate covalent attachment of the compound of formula (I) to a suitable carrier or to a linker establishing a bond to a suitable carrier. In connection with the provisos it is to be understood that, although it is sufficient that one of the given alternatives is fulfilled, this does not exclude that more than one of the given alternatives are fulfilled by the compounds of formula (I). Therefore, the term “any one of” also includes the option “at least one of”. In other words, if, e.g., R² is selected according to the proviso, this does not exclude that R¹ or R³ is selected according to the proviso. Similarly, if, e.g., R² is selected according to the proviso, this does not exclude that R^(N) is selected according to the proviso, and/or that any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a substituent R^(X) according to the proviso. As used in connection with the proviso, the term “carries a substituent R^(X)” means that the mentioned substituent may carry the substituent R^(X) at any position, which also includes the option that R^(X) is attached to R^(Y), wherein R^(Y) is attached to the mentioned substituent. This is particularly relevant in connection with the substituent R⁵. In connection with R⁵, the term “carries a substituent R^(X)” preferably means that R⁵ represents a 5- or 6-membered saturated heterocyclic ring as defined herein, which is substituted by R^(Y), wherein R^(Y), which is preferably a pyridine, is further substituted by R^(X). On the other hand, in connection with the remaining substituents, e.g., R⁴, the term “carries a substituent R^(X)” preferably means that R⁴ represents a 5- or 6-membered aromatic carbocyclic or heterocyclic ring, or a 9- or 10-membered aromatic carbobicyclic or heterobicyclic ring, wherein a substitutable carbon or heteroatom in the aforementioned cyclic rings is substituted with a substituent R^(X).

In one embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   X¹ is CH; and -   X² is CR³ with R³ being H.

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R¹ is H or F.

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R² is H, OH, NR^(C)R^(D), CN, halogen, NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)C(═O)R^(E), NR^(E)—(C₁-C₄-alkylene)-C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 4- to 6-membered saturated heterocyclyl, or 8- to 10-membered     saturated heterobicyclyl, wherein the aforementioned heterocyclic or     heterobicyclic rings comprise a nitrogen atom and optionally one or     more, same or different heteroatoms selected from O, N or S, wherein     said N- and/or S-atoms are independently oxidized or non-oxidized,     and wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X).

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₃-alkyl, NHR^(D)—C₁-C₃-alkyl,     C₁-C₂-alkoxy-C₁-C₃-alkyl, or cyclopropyl.

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R⁴ is pyridinyl, wherein each substitutable carbon atom in the     cyclic ring is independently unsubstituted or substituted by one or     more, same or different substituents R^(X).

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R⁵ is piperidine, wherein each substitutable carbon or heteroatom in     the piperidine ring is independently unsubstituted or substituted by     one or more, same or different substituents R^(Y).

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R⁵ is piperidine, wherein each substitutable carbon atom in the     piperidine ring is independently unsubstituted or substituted by one     or more, same or different substituents R^(Y); and wherein the     nitrogen atom in the piperidine ring is substituted with R^(Y) being     pyridinyl, which is unsubstituted or substituted with one or more,     same or different substituents R^(X).

In another embodiment, the present invention relates to a compound of formula (I) as defined above, wherein

-   R^(C) is H or C₁-C₂-alkyl; -   R^(D) is H or C₁-C₂-alkyl; -   R^(E) is NH₂—C₁-C₄-alkyl; -   R^(F) is H; -   R^(X) is halogen, OH, NH₂, NHCH₃, C₁-C₂-alkyl, C₁-C₂-alkoxy,     NH₂—C₁-C₂-alkyl, HO—C₁-C₂-alkyl, or two R^(X) together with the     carbon atom to which they are bonded form a 3-membered saturated     carbocyclic ring; -   R^(Y) is halogen, OH, NH₂, or a 5- or 6-membered aromatic     heterocyclyl, wherein the aforementioned heterocyclic ring comprises     one or more, same or different heteroatoms selected from O, N or S,     wherein said N- and/or S-atoms are independently oxidized or     non-oxidized, and wherein each substitutable carbon or heteroatom in     the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(X).

In a particular embodiment, the present invention relates to a compound of formula (I), wherein the compound of formula (I) is a compound selected from the group consisting of

-   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one.

In another particular embodiment, the present invention relates to a compound of formula (I), wherein the compound of formula (I) is a compound selected from the group consisting of

-   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(2-aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one; -   7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methyl     pyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-1-yl]-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one.

In another aspect, the present invention relates to a compound of formula (I)

or a salt, stereoisomer, tautomer, or N-oxide thereof, wherein

-   X¹ is CH or N; -   X² is CR³ or N; -   R¹, R² and R³ are independently H, OH, NR^(C)R^(D), CN, halogen,     C₁-C₄-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₄-alkoxy, aryloxy,     benzyloxy, C(═O)R^(E), NR^(F)C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 4- to 6-membered saturated, partially or fully unsaturated, or     aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl,     heterocyclyloxy, or heterocyclyl-C₁-C₂-alkyl, or 8- to 10-membered     saturated, partially or fully unsaturated, or aromatic carbobicyclyl     or heterobicyclyl, wherein the aforementioned heterocyclic or     heterobicyclic rings comprise one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R⁴ is a 5- or 6-membered aromatic carbocyclic or heterocyclic ring,     or a 9- or 10-membered aromatic carbobicyclic or heterobicyclic     ring, wherein the heterocyclic or heterobicyclic ring comprises at     least one nitrogen atom and optionally one or more, same or     different additional heteroatoms selected from O, N or S, wherein     said N- and/or S-atoms are independently oxidized or non-oxidized,     and wherein each substitutable carbon or heteroatom in the     aforementioned cyclic rings is independently unsubstituted or     substituted with one or more same or different substituents R^(X); -   R⁵ is a 5- or 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y);     and wherein -   R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₄-alkyl,     NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₂-alkoxy-C₁-C₄-alkyl, or a 3- or     4-membered saturated carbocyclyl or heterocyclyl, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(C) and R^(D) are independently H, C₁-C₂-alkyl, or C(═O)R^(E); or -   R^(C) and R^(D) together with the nitrogen atom to which they are     bonded form a 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the heterocyclic ring is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(E) is H, C₁-C₂-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, phenyl, benzyl,     OR^(G), or NR^(H)R^(I); or a 5- or 6-membered saturated, partially     or fully unsaturated heterocyclyl, wherein said heterocyclic ring     comprises one or more, same or different heteroatoms selected from     O, N or S, wherein said N- and/or S-atoms are independently oxidized     or non-oxidized, and wherein each substitutable carbon or heteroatom     in the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(F); -   R^(F) is H, C₁-C₂-alkyl, C₃-C₆-cycloalkyl, phenyl, benzyl, or     C(═O)NR^(H)R^(I); -   R^(G) is H, C₁-C₂-alkyl, or 5- or 6-membered aromatic carbocyclyl,     carbocyclyl-C₁-C₂-alkyl, heterocyclyl, or heterocyclyl-C₁-C₂-alkyl,     wherein the aforementioned heterocyclic rings comprise one or more,     same or different heteroatoms selected from O, N or S, wherein said     N-atoms are independently oxidized or non-oxidized; -   R^(H) and R^(I) are independently H, C₁-C₂-alkyl, or 5- or     6-membered aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl,     heterocyclyl, or heterocyclyl-C₁-C₂-alkyl, wherein the     aforementioned heterocyclic rings comprise one or more, same or     different heteroatoms selected from O, N or S, wherein said N-atoms     are independently oxidized or non-oxidized; or -   R^(H) and R^(I) together with the nitrogen atom to which they are     bonded form a 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic heterocyclic ring, wherein said     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the heterocyclic ring is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X); -   R^(X) is OH, NR^(C)R^(D), halogen, CN, NO₂, C₁-C₂-alkyl,     cyclopropyl, C₁-C₂-haloalkyl, NR^(C)R^(D)—C₁-C₄-alkyl,     R^(C)O—C₁-C₄-alkyl, C₁-C₂-alkoxy, R^(C)O—C₁-C₄-alkyloxy, C(═O)R^(E),     or two R^(X) form ═O, or two R^(X) together with the carbon atom to     which they are bonded form a 3- to 5-membered saturated, partially     or fully unsaturated, or aromatic carbocyclic ring; -   R^(Y) is halogen, CN, OH, C₁-C₂-alkyl, HO—C₁-C₂-alkyl,     C₃-C₆-cycloalkyl, C₁-C₂-alkoxy, NR^(C)R^(D), S(═O)₂NR^(C)R^(D),     C(═O)R^(E), or 5- or 6-membered saturated, partially or fully     unsaturated, or aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl,     heterocyclyl, and heterocyclyl-C₁-C₂-alkyl, wherein the     aforementioned heterocyclic rings comprise one or more, same or     different heteroatoms selected from O, N or S, wherein said N-     and/or S-atoms are independently oxidized or non-oxidized, and     wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X); or two RY     form ═O; or two RY attached to identical or neighboring carbon atoms     may form a 3-membered carbocyclic ring;     with the proviso that     either     any one of -   R¹, R², or R³ is NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 8- to 10-membered saturated, partially or fully unsaturated, or     aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X);     or     any one of -   R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is     NR^(C)R^(D)—C₁-C₄-alkyl;     or -   R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl, or C₁-C₂-alkoxy-C₁-C₄-alkyl;     or -   any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a     substituent R^(X), wherein -   R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     R^(C)O—C₁-C₄-alkyl, or R^(C)O—C₁-C₄-alkyloxy.

In a further aspect, the present invention relates to a pharmaceutical composition comprising a pharmaceutically effective amount of the compound of formula (I) as defined herein, and optionally a pharmaceutically acceptable carrier, diluent or excipient.

In yet another aspect, the present invention relates to a compound of formula (I) as defined herein or a pharmaceutical composition comprising the same as defined herein for use in medicine. In particular, the present invention relates to a compound of formula (I) as defined herein or a pharmaceutical composition comprising the same as defined herein for use in modulating STING, in particular activating STING.

In yet another aspect, the present invention relates to a compound of formula (I) as defined herein or a pharmaceutical composition comprising the same as defined herein for use in a method of treating a disease, in which the modulation of STING, in particular the activation of STING, is beneficial.

In one embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of cancer, pre-cancerous syndromes, and infectious diseases; or for use in an immunogenic composition or as vaccine adjuvant.

In another embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of inflammatory diseases, allergic diseases, and autoimmune diseases.

In a further aspect, the present invention relates to methods of treatment comprising the administration of a compound of formula (I) as defined herein or a pharmaceutical composition comprising the same as defined herein to a human or animal body.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1A-FIG. 1C show the efficacy of Example 4 in CT26 murine colon carcinoma allograft in Balb/C female mice.

FIG. 2A-FIG. 2C show the efficacy of Example 31 in CT26 murine colon carcinoma allograft in Balb/C female mice.

FIG. 3A-FIG. 3C show the efficacy of Example 57 in CT26 murine colon carcinoma allograft in Balb/C female mice.

DETAILED DESCRIPTION

In the following, preferred embodiments of the substituents in the above formula (I) are described in further detail. It is to be understood that each preferred embodiment is relevant on its own as well as in combination with other preferred embodiments. Furthermore, it is to be understood that the preferences in each case also apply to the salts, stereoisomers, tautomers, and N-oxides of the compounds of the invention.

As indicated above, the present invention relates to a compound of formula (I)

wherein

-   X¹ is CH or N; and -   X² is CR³ or N;

Accordingly, the compound of formula (I) may therefore be a compound of formula (Ia), (Ib), (Ic) or (Id) as shown below:

In a preferred embodiment, the compound of formula (I) is a compound of formula (Ia) or (Ib), in particular a compound of formula (Ia).

In another preferred embodiment of the compound of formula (I),

X² is CR³ with R³ being H.

Accordingly, the compound of formula (I) is preferably a compound of formula (Ia) or (Ib), wherein R³ is H.

In one embodiment of the invention, the compound of formula (I) is a compound of formula (Ia), wherein R³ is H. These compounds are referred to as compounds of formula (Ia*) as shown below:

In connection with the compounds according to formula (Ia), (Ib), (Ic) and (Id) as well as (Ia*), it is to be understood that the substituents R¹, R², R³, R⁴, R⁵, and R^(N) are as defined above in formula (I). Further preferred embodiments regarding these substituents are provided further below.

As indicated above, any one of the substituents R¹, R², R³, R⁴, R⁵, or R^(N) either represents or carries a functional group that is suitable for further functionalization. In particular, either

any one of

-   R¹, R², or R³ is NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 8- to 10-membered saturated, partially or fully unsaturated, or     aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X);     or     any one of -   R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is     NR^(C)R^(D)—C₁-C₄-alkyl;     or -   R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl, or C₁-C₂-alkoxy-C₁-C₄-alkyl;     or -   any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a     substituent R^(X), wherein -   R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, or     R^(C)O—C₁-C₄-alkyl.

Preferably, either any one of

-   R¹, R², or R³ is NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 8- to 10-membered saturated, partially or fully unsaturated, or     aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X);     or     any one of -   R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is     NR^(C)R^(D)—C₁-C₄-alkyl;     or -   R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl;     or -   any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a     substituent R^(X), wherein -   R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, or     R^(C)O—C₁-C₄-alkyl.

Preferably, either any one of

-   R¹, R², or R³ is NHR^(D), NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 8- to 10-membered saturated, partially or fully unsaturated, or     aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X);     or     any one of -   R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is     NR^(C)R^(D)—C₁-C₄-alkyl;     or -   R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl;     or -   any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a     substituent R^(X), wherein -   R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, or     R^(C)O—C₁-C₄-alkyl.

Preferably, any one of R², R⁴, R⁵, or RN either represents or carries a functional group that is suitable for further functionalization. In particular, either

-   R² is NHR^(D), NHR^(D)—C₁-C₄-alkyl, NH—(C₁-C₄-alkylene)-NR^(C)R^(D),     NR^(F)—(C₁-C₄-alkylene)-NHR^(D), O—(C₁-C₄-alkylene)-NHR^(D), or 8-     to 10-membered saturated heterobicyclyl, wherein the aforementioned     heterobicyclic ring comprises at least one nitrogen atom and     optionally one or more, same or different heteroatoms selected from     O, N or S, wherein said N- and/or S-atoms are independently oxidized     or non-oxidized, and wherein each substitutable carbon or heteroatom     in the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(X);     or -   R² is NR^(F)C(═O)R^(E), wherein R^(E) is NHR^(D)—C₁-C₄-alkyl;     or -   R^(N) is NHR^(D)—C₁-C₄-alkyl;     or -   any one of the substituents R², R⁴, R⁵, R^(N) carries a substituent     R^(X), wherein -   R^(X) is OH, NHR^(D), NHR^(D)—C₁-C₄-alkyl, or HO—C₁-C₄-alkyl.

More preferably, any one of R², R⁴, or R⁵ either represents or carries a functional group that is suitable for further functionalization. In particular, either

-   R² is NH₂, NH₂—C₁-C₂-alkyl, NH—(C₁-C₃-alkylene)-NH₂,     NH—(C₁-C₃-alkylene)-NHCH₃, NH—(C₁-C₃-alkylene)-N(CH₃)₂,     O—(C₁-C₃-alkylene)-NH₂, or O—(C₁-C₃-alkylene)-NHCH₃;     or -   R² is NHC(═O)R^(E), wherein R^(E) is NH₂—C₁-C₄-alkyl;     or -   R^(N) is NH₂—C₁-C₃-alkyl;     or -   any one of the substituents R², R⁴, or R⁵ carries a substituent     R^(X), wherein -   R^(X) is OH, NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, or HO—C₁-C₂-alkyl.

In connection with the above options for the functional groups, it is to be understood that, although it is sufficient that one of the given alternatives is fulfilled, this does not exclude that more than one of the given alternatives are fulfilled by the compounds of formula (I). As used in connection with the above options, the term “carries a substituent R^(X)” means that the mentioned substituent may carry the substituent R^(X) at any position, which also includes the option that R^(X) is attached to R^(Y), wherein R^(Y) is attached to the mentioned substituent. This is particularly relevant in connection with the substituent R⁵. In connection with R⁵, the term “carries a substituent R^(X)” preferably means that R⁵ represents a 5- or 6-membered saturated heterocyclic ring as defined herein, which is substituted by R^(Y), wherein R^(Y), which is preferably a pyridine, is further substituted by R^(X). On the other hand, in connection with the remaining substituents, e.g., R⁴, the term “carries a substituent R^(X)” preferably means that R⁴ represents a 5- or 6-membered aromatic carbocyclic or heterocyclic ring, or a 9- or 10-membered aromatic carbobicyclic or heterobicyclic ring, wherein a substitutable carbon or heteroatom in the aforementioned cyclic rings is substituted with a substituent R^(X).

The following substituents are preferred in connection with the compound of formula (I), as well as in connection with the compounds of formula (Ia), (Ib), (Ic) and (Id), especially in connection with the compounds of formula (Ia) and (Ib), and in particular in connection with the compounds according to formula (Ia), and in connection with the compounds according to formula (Ia*).

In one preferred embodiment,

-   R¹ is H or F.

Accordingly, if the compound of formula (I) is a compound of formula (Ia*) as defined above, it may be represented by the formula (Ia*.1) or (Ia*.2) as shown below:

Thus, in a particularly preferred embodiment according to the present invention, the compound of formula (I) is a compound of formula (Ia*.1) or (Ia*.2).

The following substituents are therefore not only preferred in connection with the compound of formula (I) as well as in connection with the compounds of (la), (Ib), (Ic) and (Id), especially in connection with the compounds of formula (Ia) and (Ib), and in particular in connection with the compounds according to formula (Ia), and in connection with the compounds according to formula (Ia*), but especially preferably in connection with the compounds of formula (Ia*.1) and (Ia*.2).

In one preferred embodiment,

-   R² is H, OH, NR^(C)R^(D), CN, halogen, NR^(C)R^(D)—C₁-C₄-alkyl,     C₁-C₄-alkoxy, NR^(F)C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 4- to 6-membered saturated, partially or fully unsaturated, or     aromatic heterocyclyl or 8- to 10-membered saturated, partially or     fully unsaturated, or aromatic heterobicyclyl, wherein the     aforementioned heterocyclic or heterobicyclic rings comprise a     nitrogen atom and optionally one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X).

In one preferred embodiment,

-   R² is H, OH, NR^(C)R^(D), CN, halogen, NR^(C)R^(D)—C₁-C₄-alkyl,     NR^(F)C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E),     NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D),     or 4- to 6-membered saturated heterocyclyl, or 8- to 10-membered     saturated heterobicyclyl, wherein the aforementioned heterocyclic or     heterobicyclic rings comprise a nitrogen atom and optionally one or     more, same or different heteroatoms selected from O, N or S, wherein     said N- and/or S-atoms are independently oxidized or non-oxidized,     and wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X).

In a more preferred embodiment,

-   R² is H, NHR^(D), halogen, NHR^(D)—C₁-C₄-alkyl, NHC(═O)R^(E),     NH—(C₁-C₄-alkylene)-C(═O)R^(E), NH—(C₁-C₄-alkylene)-NR^(C)R^(D),     O—(C₁-C₄-alkylene)-NR^(C)R^(D), or 4- to 6-membered saturated     heterocyclyl, or 8- to 10-membered saturated heterobicyclyl, wherein     the aforementioned heterocyclic or heterobicyclic rings comprise a     nitrogen atom and optionally one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned groups is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X).

In an even more preferred embodiment,

-   R² is H, NHR^(D), halogen, NHR^(D)—C₁-C₄-alkyl, NHC(═O)R^(E),     NH—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D), or     4- to 6-membered saturated heterocyclyl, or 9- to 10-membered     saturated heterobicyclyl, wherein the aforementioned heterocyclic or     heterobicyclic rings comprise one or two nitrogen atoms, wherein     said N-atoms are independently oxidized or non-oxidized, and wherein     each substitutable carbon or heteroatom in the aforementioned groups     is independently unsubstituted or substituted with one or more, same     or different substituents R^(X).

If an R^(X) group is present at R², it is preferred that

-   R^(X) is halogen, OH, NH₂, NHCH₃, C₁-C₂-alkyl, C₁-C₂-alkoxy,     NH₂—C₁-C₂-alkyl, HO—C₁-C₂-alkyl, or two R^(X) together with the     carbon atom to which they are bonded form a 3-membered saturated     carbocyclic ring.

In particular, it is preferred if an R^(X) group is present at R² that at least one R^(X) is OH, NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, HO—C₁-C₂-alkyl. Such groups are particularly suitable as functional groups to facilitate covalent attachment of the compound of formula (I) to a suitable carrier or to a linker establishing a bond to a suitable carrier.

Furthermore, the following substituent definitions are preferred in connection with the above definition of R²:

R^(C) is H or C₁-C₂-alkyl; R^(D) is H or C₁-C₂-alkyl; R^(E) is NH₂—C₁-C₄-alkyl;

R^(E) is H.

Preferred R² groups are selected from the group consisting of H, Br, F, Cl, NH₂,

Particularly preferred R² groups are selected from the group consisting of H, Br, F, NH₂,

In one preferred embodiment,

-   R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₃-alkyl, NHR^(D)—C₁-C₃-alkyl,     C₁-C₂-alkoxy-C₁-C₃-alkyl, cyclopropyl or a 4-membered saturated     heterocyclyl, wherein said heterocyclic ring comprises one or more,     same or different heteroatoms selected from O, N or S, wherein said     N and/or S-atoms are independently oxidized or non-oxidized, and     wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X).

If a R^(X) group is present at R^(N), it is preferred that R^(X) is OH or two R^(X) form ═O.

In connection with the above embodiment with regard to R^(N) it is preferred that R^(D) is H.

In one preferred embodiment,

-   R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₃-alkyl, NHR^(D)—C₁-C₃-alkyl,     C₁-C₂-alkoxy-C₁-C₃-alkyl, or cyclopropyl.

In a more preferred embodiment,

-   R^(N) is H, C₁-C₃-alkyl, HO(C═O)—C₁-alkyl, or cyclopropyl.

In a more preferred embodiment,

-   R^(N) is H, CH₃, isopropyl or cyclopropyl.

In another more preferred embodiment,

R^(N) is H, C₁-C₂-alkyl, HO(C═O)—C₁-alkyl, or cyclopropyl. In a more preferred embodiment,

-   R^(N) is H, CH₃ or cyclopropyl.

In one particularly preferred embodiment,

-   R^(N) is CH₃.

In another particularly preferred embodiment,

-   R^(N) is cyclopropyl.

In one preferred embodiment,

-   R⁴ is pyridinyl, wherein each substitutable carbon atom in the     cyclic ring is independently unsubstituted or substituted by one or     more, same or different substituents R^(X).

If at least one R^(X) group is present at R⁴, it is preferred that

-   R^(X) is NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, C₁-C₂-alkyl, or C₁-C₂-alkoxy.

It is preferred that the pyridinyl is substituted by one or more, same or different substituents RX as defined above.

Thus, in a more preferred embodiment,

-   R⁴ is methylpyridinyl, aminopyridinyl or methoxypyridinyl.

Preferably, R⁴ is any one of the following substituted pyridinyl rings:

In a particularly preferred embodiment,

-   R⁴ is methylpyridinyl.

Particularly preferably,

-   R⁴ is:

In one embodiment,

-   R⁵ is a 5- or 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more nitrogen atoms, wherein said     N-atoms are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y).

In a more preferred embodiment,

-   R⁵ is a 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more nitrogen atoms, wherein said     N-atoms are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y).

In a yet more preferred embodiment,

-   R⁵ is piperidine, wherein each substitutable carbon or heteroatom in     the piperidine ring is independently unsubstituted or substituted by     one or more, same or different substituents R^(Y).

If at least one RY group is present at R⁵, it is preferred that

-   R^(Y) is halogen, OH, NH₂, or a 5- or 6-membered aromatic     heterocyclyl, wherein the aforementioned heterocyclic ring comprises     one or more, same or different heteroatoms selected from O, N or S,     wherein said N- and/or S-atoms are independently oxidized or     non-oxidized, and wherein each substitutable carbon or heteroatom in     the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(X).

It is preferred that the nitrogen atom of the piperidinyl group is substituted by a substituent R^(Y), wherein

-   R^(Y) is a 5- or 6-membered aromatic heterocyclyl, wherein the     aforementioned heterocyclic ring comprises one or more, same or     different heteroatoms selected from O, N or S, wherein said N-     and/or S-atoms are independently oxidized or non-oxidized, and     wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X).

If at least one R^(X) group is present at R^(Y), it is preferred that

-   R^(X) is F, Cl, NH₂, NHCH₃, N(CH₃)₂, NH₂—C₁-C₂-alkyl,     HO—C₁-C₄-alkyl, C₁-C₂-alkyl, C₁-C₂-haloalkyl, or C₁-C₂-alkoxy.

In one particularly preferred embodiment,

-   R⁵ is piperidine, wherein each substitutable carbon or heteroatom in     the piperidine ring is independently unsubstituted or substituted by     one or more, same or different substituents R^(Y); and wherein the     nitrogen atom of the piperidine group is substituted by R^(Y) being     a 5- or 6-membered aromatic heterocyclyl, wherein the aforementioned     heterocyclic ring comprises one or more, same or different     heteroatoms selected from O, N or S, wherein said N- and/or S-atoms     are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom in the aforementioned     heterocyclic ring is independently unsubstituted or substituted with     one or more, same or different substituents R^(X), wherein     preferably R^(X) is F, Cl, NH₂, NHCH₃, N(CH₃)₂, NH₂—C₁-C₂-alkyl,     HO—C₁-C₄-alkyl, C₁-C₂-alkyl, C₁-C₂-haloalkyl, or C₁-C₂-alkoxy.

Accordingly, particularly preferred R⁵ groups are selected from the group consisting of

In one preferred embodiment,

-   R⁵ is a 5- or 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more nitrogen atoms, wherein said     N-atoms are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y).

In a more preferred embodiment,

-   R⁵ is a 6-membered saturated heterocyclic ring, wherein said     heterocyclic ring comprises one or more nitrogen atoms, wherein said     N-atoms are independently oxidized or non-oxidized, and wherein each     substitutable carbon or heteroatom is independently unsubstituted or     substituted with one or more, same or different substituents R^(Y).

In a yet more preferred embodiment,

-   R⁵ is piperidine, wherein each substitutable carbon or heteroatom in     the piperidine ring is independently unsubstituted or substituted by     one or more, same or different substituents R^(Y).

If at least one RY group is present at R⁵, it is preferred that

-   R^(Y) is halogen, OH, NH₂, or a 5- or 6-membered aromatic     heterocyclyl, wherein the aforementioned heterocyclic ring comprises     one or more, same or different heteroatoms selected from O, N or S,     wherein said N- and/or S-atoms are independently oxidized or     non-oxidized, and wherein each substitutable carbon or heteroatom in     the aforementioned groups is independently unsubstituted or     substituted with one or more, same or different substituents R^(X).

It is preferred that the nitrogen atom of the piperidinyl group is substituted by a substituent R^(Y), wherein

-   R^(Y) is a 6-membered aromatic heterocyclyl, wherein the     aforementioned heterocyclic ring comprises one or more, same or     different heteroatoms selected from O, N or S, wherein said N-     and/or S-atoms are independently oxidized or non-oxidized, and     wherein each substitutable carbon or heteroatom in the     aforementioned groups is independently unsubstituted or substituted     with one or more, same or different substituents R^(X).

It is more preferred that the nitrogen atom of the piperidinyl group is substituted by a substituent R^(Y), wherein

-   R^(Y) is pyridinyl, wherein each substitutable carbon atom is     independently unsubstituted or substituted with one or more, same or     different substituents R^(X).

If at least one R^(X) group is present at R^(Y), it is preferred that

-   R^(X) is NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, C₁-C₂-alkyl, or C₁-C₂-alkoxy.

In one particularly preferred embodiment,

-   R⁵ is piperidine, wherein each substitutable carbon or heteroatom in     the piperidine ring is independently unsubstituted or substituted by     one or more, same or different substituents R^(Y); and wherein the     nitrogen atom of the piperidine group is substituted by a     substituent R^(Y) being pyridinyl, which is unsubstituted or     substituted with one or more, same or different substituents R^(X),     wherein preferably R^(X) is NH₂, NHCH₃, NH₂—C₁-alkyl, or C₁-alkyl.

In another particularly preferred embodiment,

-   R⁵ is piperidine, wherein the nitrogen atom of the piperidine group     is substituted by a substituent R^(Y) being pyridinyl, which is     unsubstituted or substituted with one or more, same or different     substituents R^(X), wherein preferably R^(X) is NH₂, NHCH₃,     NH₂—C₁-alkyl, or C₁-alkyl. It is especially preferred that R⁵ is

wherein $ marks the connection to the substituent R^(Y). As indicated above, R^(Y) is preferably pyridinyl, which is unsubstituted or substituted with one or more, same or different substituents R^(X), wherein R^(X) is NH₂, NHCH₃, NH₂—C₁-alkyl, or C₁-alkyl. In particular, R^(Y) is

wherein R^(Y)-1 and R^(Y)-2 may be unsubstituted or substituted with one or more, same or different substituents R^(X), wherein R^(X) is NH₂, NHCH₃, NH₂—C₁-alkyl, or C₁-alkyl, in particular CH₃.

Accordingly, particularly preferred R⁵ groups are selected from the group consisting of

Thus, particularly preferred compounds of the invention are compounds of formula (Ia*) as compiled in the tables below.

Table 1

Compounds of the formula (Ia*.1), in which R^(N) is CH₃, R⁴ is R⁴-1, and R² and R⁵ correspond in each case to one row of Table A

Table 2

Compounds of the formula (Ia*.1), in which R^(N) is CH₃, R⁴ is R⁴-2, and R² and R⁵ correspond in each case to one row of Table A.

Table 3

Compounds of the formula (Ia*.1), in which R^(N) is cyclopropyl, R⁴ is R⁴-1, and R² and R⁵ correspond in each case to one row of Table A.

Table 4

Compounds of the formula (Ia*.1), in which R^(N) is cyclopropyl, R⁴ is R⁴-2, and R² and R⁵ correspond in each case to one row of Table A.

Table 5

Compounds of the formula (Ia*.2), in which R^(N) is CH₃, R⁴ is R⁴-1, and R² and R⁵ correspond in each case to one row of Table A

Table 6

Compounds of the formula (Ia*.2), in which R^(N) is CH₃, R⁴ is R⁴-2, and R² and R⁵ correspond in each case to one row of Table A.

Table 7

Compounds of the formula (Ia*.2), in which R^(N) is cyclopropyl, R⁴ is R⁴-1, and R² and R⁵ correspond in each case to one row of Table A.

Table 8

Compounds of the formula (Ia*.2), in which R^(N) is cyclopropyl, R⁴ is R⁴-2, and R² and R⁵ correspond in each case to one row of Table A.

TABLE A No. R² R⁵ A-1 H R⁵-1 A-2 H R⁵-2 A-3 H R⁵-3 A-4 H R⁵-4 A-5 H R⁵-5 A-6 BR R⁵-1 A-7 BR R⁵-2 A-8 BR R⁵-3 A-9 BR R⁵-4 A-10 BR R⁵-5 A-11 F R⁵-1 A-12 F R⁵-2 A-13 F R⁵-3 A-14 F R⁵-4 A-15 F R⁵-5 A-16 NH₂ R⁵-1 A-17 NH₂ R⁵-2 A-18 NH₂ R⁵-3 A-19 NH₂ R⁵-4 A-20 NH₂ R⁵-5 A-21 R²-1 R⁵-1 A-22 R²-1 R⁵-2 A-23 R²-1 R⁵-3 A-24 R²-1 R⁵-4 A-25 R²-1 R⁵-5 A-26 R²-2 R⁵-1 A-27 R²-2 R⁵-2 A-28 R²-2 R⁵-3 A-29 R²-2 R⁵-4 A-30 R²-2 R⁵-5 A-31 R²-3 R⁵-1 A-32 R²-3 R⁵-2 A-33 R²-3 R⁵-3 A-34 R²-3 R⁵-4 A-35 R²-3 R⁵-5 A-36 R²-4 R⁵-1 A-37 R²-4 R⁵-2 A-38 R²-4 R⁵-3 A-39 R²-4 R⁵-4 A-40 R²-4 R⁵-5 A-41 R²-5 R⁵-1 A-42 R²-5 R⁵-2 A-43 R²-5 R⁵-3 A-44 R²-5 R⁵-4 A-45 R²-5 R⁵-5 A-46 R²-6 R⁵-1 A-47 R²-6 R⁵-2 A-48 R²-6 R⁵-3 A-49 R²-6 R⁵-4 A-50 R²-6 R⁵-5 A-51 R²-7 R⁵-1 A-52 R²-7 R⁵-2 A-53 R²-7 R⁵-3 A-54 R²-7 R⁵-4 A-55 R²-7 R⁵-5 A-56 R²-8 R⁵-1 A-57 R²-8 R⁵-2 A-58 R²-8 R⁵-3 A-59 R²-8 R⁵-4 A-60 R²-8 R⁵-5 A-61 R²-9 R⁵-1 A-62 R²-9 R⁵-2 A-63 R²-9 R⁵-3 A-64 R²-9 R⁵-4 A-65 R²-9 R⁵-5 A-66 R²-10 R⁵-1 A-67 R²-10 R⁵-2 A-68 R²-10 R⁵-3 A-69 R²-10 R⁵-4 A-70 R²-10 R⁵-5 A-71 R²-11 R⁵-1 A-72 R²-11 R⁵-2 A-73 R²-11 R⁵-3 A-74 R²-11 R⁵-4 A-75 R²-11 R⁵-5 A-76 R²-12 R⁵-1 A-77 R²-12 R⁵-2 A-78 R²-12 R⁵-3 A-79 R²-12 R⁵-4 A-80 R²-12 R⁵-5 A-81 R²-13 R⁵-1 A-82 R²-13 R⁵-2 A-83 R²-13 R⁵-3 A-84 R²-13 R⁵-4 A-85 R²-13 R⁵-5 A-86 R²-14 R⁵-1 A-87 R²-14 R⁵-2 A-88 R²-14 R⁵-3 A-89 R²-14 R⁵-4 A-90 R²-14 R⁵-5 A-91 R²-15 R⁵-1 A-92 R²-15 R⁵-2 A-93 R²-15 R⁵-3 A-94 R²-15 R⁵-4 A-95 R²-15 R⁵-5 A-96 R²-16 R⁵-1 A-97 R²-16 R⁵-2 A-98 R²-16 R⁵-3 A-99 R²-16 R⁵-4 A-100 R²-16 R⁵-5 A-101 R²-17 R⁵-1 A-102 R²-17 R⁵-2 A-103 R²-17 R⁵-3 A-104 R²-17 R⁵-4 A-105 R²-17 R⁵-5

It has been found that the compounds as defined in the above tables are particularly advantageous as STING agonists, and may therefore particularly advantageously be used in the pharmaceutical compositions of the present invention as well as the medical uses as defined herein.

Therefore, the compound of formula (I) of the invention is preferably a compound according to any one of tables 1-8, and the present invention preferably relates to pharmaceutical compositions comprising the same and to medical uses thereof.

In a particularly preferred embodiment, the compound of formula (I) is a compound selected from the group consisting of

-   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one.

In another particularly preferred embodiment, the compound of formula (I) is a compound selected from the group consisting of

-   3-({[(2-aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one; -   7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methyl     pyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-1-yl]-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one.

In an even more particularly preferred embodiment, the compound of formula (I) is a compound selected from the group consisting of

-   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(2-aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one; -   7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(methylami     no)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one; -   1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methyl     pyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-1-yl]-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; -   1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one.

Preferred compounds of the invention comprise a stereocenter at the R⁵ substituent in S-configuration. The configuration of this stereocenter can be predefined in the preparation of the compounds when introducing the substituent R⁵ by selecting a precursor with the respective configuration (see, e.g., Example 1).

In certain scenarios, a further stereocenter may be present at another position of the molecule.

In case the configuration of the further stereocenter can also be predefined by using a suitable precursor in the preparation of the compounds, the exact structure of the resulting molecule is predefined as well, meaning that a clear R/S-nomenclature can be provided for both stereocenters in the molecule.

However, if the further stereocenter is introduced based on a precursor in racemic form (i.e. not enantiomerically pure), two diastereoisomers will be formed, which can be separated afterwards. In such scenarios, the nomenclature of the additional stereocenter is provided arbitrarily herein. In other words, for example in case of the diastereoisomer pair 7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one and 7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (see Examples 56 and 57), it is clear that both compounds are obtained and separated, but it is not clear which compound has which structure as further experimental work (such as X-ray crystallography) would be needed to determine this. Thus, if it is referred herein to 7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one this could also be 7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one and vice versa.

Definitions

The term “compound(s) of the present invention” is to be understood as equivalent to the term “compound(s) according to the invention”, and also covers a salt, stereoisomer, tautomer or N-oxide thereof.

The compounds according to the invention may be amorphous or may exist in one or more different crystalline states (polymorphs), which may have different macroscopic properties such as stability or show different biological properties such as activities. The present invention relates to amorphous and crystalline forms of compounds of formula (I), mixtures of different crystalline states of the compounds of formula (I), as well as amorphous or crystalline salts thereof.

Salts of the compounds according to the invention are preferably pharmaceutically acceptable salts, such as those containing counterions present in drug products listed in the US FDA Orange Book database. They can be formed in a customary manner, e.g., by reacting the compound with an acid of the anion in question, if the compounds according to the invention have a basic functionality, or by reacting acidic compounds according to the invention with a suitable base.

Suitable cationic counterions are in particular the ions of the alkali metals, preferably lithium, sodium and potassium, of the alkaline earth metals, preferably calcium, magnesium and barium, and of the transition metals, preferably manganese, copper, silver, zinc and iron, and also ammonium (NH₄ ⁺) and substituted ammonium in which one to four of the hydrogen atoms are replaced by C₁-C₄-alkyl, C₁-C₄-hydroxyalkyl, C₁-C₄-alkoxy, C₁-C₄-alkoxy-C₁-C₄-alkyl, hydroxy-C₁-C₄-alkoxy-C₁-C₄-alkyl, phenyl or benzyl. Examples of substituted ammonium ions comprise methylammonium, isopropylammonium, dimethylammonium, diisopropylammonium, trimethylammonium, tetramethylammonium, tetraethylammonium, tetrabutylammonium, 2-hydroxyethylammonium, 2-(2-hydroxyethoxy)ethyl-ammonium, bis(2-hydroxyethyl)ammonium, benzyltrimethylammonium and benzyltriethylammonium, furthermore the cations of 1,4-piperazine, meglumine, benzathine and lysine.

Suitable anionic counterions are in particular chloride, bromide, hydrogensulfate, sulfate, dihydrogenphosphate, hydrogenphosphate, phosphate, nitrate, bicarbonate, carbonate, hexafluorosilicate, hexafluorophosphate, benzoate, and the anions of C₁-C₄-alkanoic acids, preferably formate, acetate, propionate and butyrate, furthermore lactate, gluconate, and the anions of poly acids such as succinate, oxalate, maleate, fumarate, malate, tartrate and citrate, furthermore sulfonate anions such as besylate (benzenesulfonate), tosylate (p-toluenesulfonate), napsylate (naphthalene-2-sulfonate), mesylate (methanesulfonate), esylate (ethanesulfonate), and ethanedisulfonate. They can be formed by reacting compounds according to the invention that have a basic functionality with an acid of the corresponding anion.

Depending on the substitution pattern, the compounds according to the invention may have one or more centers of chirality, including axial chirality. The invention provides both, pure enantiomers or pure diastereomers, of the compounds according to the invention, and their mixtures, including racemic mixtures. Suitable compounds according to the invention also include all possible geometrical stereoisomers (cis/trans isomers or E/Z isomers) and mixtures thereof. E/Z-isomers may be present with respect to, e.g., an alkene, carbon-nitrogen double-bond or amide group.

Tautomers may be formed, if a substituent is present at the compound of formula (I), which allows for the formation of tautomers such as keto-enol tautomers, imine-enamine tautomers, amide-imidic acid tautomers or the like. Furthermore, the core structure comprising the 6-membered ring that contains the ═O substituent principally allows for keto-enol-tautomerization.

The term “N-oxide” includes any compound of the present invention which has at least one tertiary nitrogen atom that is oxidized to a N-oxide moiety.

The term “substituted”, as used herein, means that a hydrogen atom bonded to a designated atom is replaced with a specified substituent, provided that the substitution results in a stable or chemically feasible compound. Unless otherwise indicated, a substituted atom may have one or more substituents and each substituent is independently selected.

The term “substitutable”, when used in reference to a designated atom, means that attached to the atom is a hydrogen, which can be replaced with a suitable substituent.

In connection with the above term “substitutable”, in particular with regard to the expression “wherein each substitutable carbon or heteroatom is independently unsubstituted or substituted with one or more, same or different substituents” it is to be understood that this term covers all possible options wherein e.g. carbon and heteroatoms are independently unsubstituted or substituted or wherein e.g. only carbon or only heteroatoms are independently unsubstituted or substituted with one or more, same or different substituents.

When it is referred to certain atoms or moieties being substituted with “one or more” substituents, the term “one or more” is intended to cover at least one substituent, e.g. 1 to 10 substituents, preferably 1, 2, 3, 4, or 5 substituents, more preferably 1, 2, or 3 substituents, most preferably 1, or 2 substituents. When neither the term “unsubstituted” nor “substituted” is explicitly mentioned concerning a moiety, said moiety is to be considered as unsubstituted.

The organic moieties mentioned in the above definitions of the variables are—like the term halogen—collective terms for individual listings of the individual group members. The prefix C_(n)-C_(m) indicates in each case the possible number of carbon atoms in the group.

The term “halogen” denotes in each case fluorine, bromine, chlorine or iodine, in particular fluorine, chlorine, or bromine.

The term “alkyl” as used herein denotes in each case a straight-chain or branched alkyl group having usually from 1 to 6 carbon atoms, preferably 1 to 5 or 1 to 4 carbon atoms, more preferably 1 to 3 or 1 or 2 carbon atoms. Examples of an alkyl group are methyl, ethyl, n-propyl, isopropyl, n-butyl, 2-butyl, iso-butyl, tert-butyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, and 1-ethyl-2-methylpropyl.

The term “haloalkyl” as used herein denotes in each case a straight-chain or branched alkyl group having usually from 1 to 6 carbon atoms, frequently 1 to 5 or 1 to 4 carbon atoms, preferably 1 to 3 or 1 or 2 carbon atoms, wherein the hydrogen atoms of this group are partially or totally replaced with halogen atoms. Preferred haloalkyl moieties are selected from C₁-C₄-haloalkyl, more preferably from C₁-C₃-haloalkyl or C₁-C₂-haloalkyl, in particular from C₁-C₂-fluoroalkyl such as fluoromethyl, difluoromethyl, trifluoromethyl, 1-fluoroethyl, 2-fluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, pentafluoroethyl, and the like.

The term “alkenyl” as used herein denotes in each case an unsaturated hydrocarbon group having usually 2 to 6, preferably 2 to 4 carbon atoms comprising at least one carbon-carbon double bond in any position, e.g. vinyl (ethenyl), allyl (2-propen-1-yl), 1-propen-1-yl, 2-propen-2-yl, methallyl (2-methylprop-2-en-1-yl), 2-buten-1-yl, 3-buten-1-yl, 2-penten-1-yl, 3-penten-1-yl, 4-penten-1-yl, 1-methylbut-2-en-1-yl, 2-ethylprop-2-en-1-yl and the like. If geometric isomers are possible with regard to the double bond, the present invention relates to both, the E- and Z-isomers. Preferred alkenyl groups according to the invention are terminal alkenyl groups. The bonding of vinyl is exemplified below:

The term “haloalkenyl” as used herein refers to an alkenyl group as defined above, wherein the hydrogen atoms are partially or totally replaced with halogen atoms.

The term “alkynyl” as used herein denotes in each case an unsaturated hydrocarbon group having usually 2 to 6, preferably 2 to 5 or 2 to 4 carbon atoms, more preferably 2 to 3 carbon atoms, comprising at least one carbon-carbon triple bond in any position, e.g. ethynyl, propargyl (2-propyn-1-yl), 1-propyn-1-yl, 1-methylprop-2-yn-1-yl), 2-butyn-1-yl, 3-butyn-1-yl, 1-pentyn-1-yl, 3-pentyn-1-yl, 4-pentyn-1-yl, 1-methylbut-2-yn-1-yl, 1-ethylprop-2-yn-1-yl and the like.

The term “haloalkynyl” as used herein refers to an alkynyl group as defined above, wherein the hydrogen atoms are partially or totally replaced with halogen atoms.

The term “alkoxy” as used herein denotes in each case a straight-chain or branched alkyl group which is bonded via an oxygen atom and has usually from 1 to 6 carbon atoms, preferably 1 to 2 carbon atoms, more preferably 1 carbon atom. Examples of an alkoxy group are methoxy, ethoxy, n-propoxy, iso-propoxy, n-butyloxy, 2-butyloxy, iso-butyloxy, tert.-butyloxy, and the like.

The term “haloalkoxy” as used herein denotes in each case a straight-chain or branched alkoxy group having from 1 to 6 carbon atoms, preferably 1 to 2 carbon atoms, more preferably 1 carbon atom, wherein the hydrogen atoms of this group are partially or totally replaced with halogen atoms, in particular fluorine atoms. Preferred haloalkoxy moieties include C₁-haloalkoxy, in particular C₁-fluoroalkoxy, such as trifluoromethoxy and the like.

The term “alkoxyalkyl” as used herein refers to an alkoxy group, preferably a C₁-C₄-alkoxy group, more preferably a C₁-C₂-alkoxy group which is bonded to the remainder of the molecule via an alkyl group preferably a C₁-C₄-alkyl group, more preferably a C₁-C₂-alkyl group. Preferred examples include methoxymethyl, ethoxymethyl, methoxyethyl, and ethoxyethyl.

The term “R^(C)O-alkyl” as used herein refers to a R^(C)O group, which is bonded to the remainder of the molecule via an alkyl group preferably a C₁-C₄-alkyl group, more preferably a C₁-C₂-alkyl group. Preferred examples include hydroxymethyl and hydroxyethyl if R^(C) is H. Other examples include alkoxyalkyl groups as defined above.

The term “HO-alkyl” as used herein refers to a OH group, which is bonded to the remainder of the molecule via an alkyl group preferably a C₁-C₄-alkyl group, more preferably a C₁-C₂-alkyl group. Preferred examples include hydroxymethyl and hydroxyethyl.

The term “NR^(C)R^(D)-alkyl” as used herein refers to an aminoalkyl group, i.e. to an amino group NR^(C)R^(D) which is bonded to the remainder of the molecule via an alkyl group, preferably a C₁-C₄-alkyl group, more preferably a C₁-C₂-alkyl group. Preferred examples include aminomethyl and aminoethyl if R^(C) and R^(D) are H.

The term “HO(C═O)—C₁-C₄-alkyl” as used herein refers to a carboxylalkyl group, i.e. to a carboxyl group C(═O)OH which is bonded to the remainder of the molecule via an alkyl group, preferably a C₁-C₄-alkyl group, more preferably a C₁-C₂-alkyl group. Preferred examples include carboxylmethyl and carboxylethyl.

The term “cycloalkyl” as used herein denotes in each case a monocyclic cycloaliphatic radical having usually from 3 to 10 or from 3 to 6 carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl and cyclodecyl or cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

The term “carbocyclic” or “carbocyclyl” includes, unless otherwise indicated, in general a 3- to 9-membered, preferably a 4- to 8-membered or a 3- to 6-membered or a 5- to 7-membered, more preferably a 5- or 6-membered monocyclic ring comprising 3 to 9, preferably 4 to 8 or 3 to 6 or 5 to 7, more preferably 5 or 6 carbon atoms. The carbocycle may be saturated, partially or fully unsaturated, or aromatic, wherein saturated means that only single bonds are present, and partially or fully unsaturated means that one or more double bonds may be present in suitable positions, while the Hückel rule for aromaticity is not fulfilled, whereas aromatic means that the Hückel (4n+2) rule is fulfilled. The term “carbocycle” or “carbocyclyl”, unless otherwise indicated, may therefore cover inter alia cycloalkyl, cycloalkenyl, as well as phenyl. Preferably, the term “carbocycle” covers cycloalkyl and cycloalkenyl groups, for example cyclopropane, cyclobutane, cyclopentane and cyclohexane rings.

The term “carbobicyclic” or “carbobicyclyl” includes in general 6 to 14-membered, preferably 7- to 12-membered or 8- to 10-membered, more preferably 9- or 10-membered bicyclic rings comprising 6 to 14, preferably 7 to 12 or 8 to 10, more preferably 9 or 10 carbon atoms. The carbobicycle may be saturated, partially or fully unsaturated, or aromatic, wherein saturated means that only single bonds are present, and partially or fully unsaturated means that one or more double bonds may be present in suitable positions, while the Hückel rule for aromaticity is not fulfilled, whereas aromatic means that the Hückel (4n+2) rule is fulfilled. Preferably, the term “aromatic” in connection with the carbobicyclic ring means that both rings of the bicyclic moiety are aromatic, so that, e.g., 8 π electrons are present in case of a 10-membered aromatic carbobicyclic ring. The term “carbobicyclic” or “carbobicyclyl”, unless otherwise indicated, may therefore cover inter alia bicycloalkyl, bicycloalkenyl, as well as bicyclic aromatic groups, for example bicyclohexane (decalin), bicycloheptane (such as norbornane), bicyclooctane (such as bicyclo[2.2.2]octane, bicyclo[3.2.1]octane or bicyclo[4.2.0]octane), bicyclononane (such as bicyclo[3.3.1]nonane or bicyclo[4.3.0]nonane), bicyclodecane (such as bicyclo[4.4.0]decane), bicycloundecane (such as bicyclo[3.3.3]undecane), norbornene, naphthalene and the like. Preferably, the carbobicycle is a fused carbobicycle, which is preferably aromatic, for example naphthalene.

The term “carbocyclyloxy” includes a carbocyclic ring or carbocyclyl which is bonded to the remainder of the molecule via an oxygen atom.

The term “heterocyclic” or “heterocyclyl” includes, unless otherwise indicated, in general a 3- to 9-membered, preferably a 4- to 8-membered or 5- to 7-membered, more preferably 5- or 6-membered, in particular 6-membered monocyclic ring. The heterocycle may be saturated, partially or fully unsaturated, or aromatic, wherein saturated means that only single bonds are present, and partially or fully unsaturated means that one or more double bonds may be present in suitable positions, while the Hückel rule for aromaticity is not fulfilled, whereas aromatic means that the Hückel (4n+2) rule is fulfilled. The heterocycle typically comprises one or more, e.g. 1, 2, 3, or 4, preferably 1, 2, or 3 heteroatoms selected from N, O and S as ring members, where S-atoms as ring members may be present as S, SO or SO₂. The remaining ring members are carbon atoms. In a preferred embodiment, the heterocycle is an aromatic heterocycle, preferably a 5- or 6-membered aromatic heterocycle comprising one or more, e.g. 1, 2, 3, or 4, preferably 1, 2, or 3 heteroatoms selected from N, O and S as ring members, where S-atoms as ring members may be present as S, SO or SO₂. Examples of aromatic heterocycles are provided below in connection with the definition of “hetaryl”. “Hetaryls” or “heteroaryls” are covered by the term “heterocycles”. The saturated or partially or fully unsaturated heterocycles usually comprise 1, 2, 3, 4 or 5, preferably 1, 2 or 3 heteroatoms selected from N, O and S as ring members, where S-atoms as ring members may be present as S, SO or SO₂. The skilled person is aware that S, SO or SO₂ is to be understood as follows:

Further, a skilled person is aware that resonance structures of the oxidized forms may be possible. Saturated heterocycles include, unless otherwise indicated, in general 3- to 9-membered, preferably 4- to 8-membered or 5- to 7-membered, more preferably 5- or 6-membered monocyclic rings comprising 3 to 9, preferably 4 to 8 or 5 to 7, more preferably 5 or 6 atoms comprising at least one heteroatom, such as pyrrolidine, tetrahydrothiophene, tetrahydrofuran, piperidine, tetrahydropyran, dioxane, morpholine or piperazine.

The term “heterocyclyloxy” includes a heterocyclic ring or heterocyclyl which is bonded to the remainder of the molecule via an oxygen atom.

The term “heterobicyclic” or “heterobicyclyl” includes, unless otherwise indicated, in general 6 to 14-membered, preferably 7- to 12-membered or 8- to 10-membered, more preferably 9- or 10-membered bicyclic rings. The heterobicycle may be saturated, partially or fully unsaturated, or aromatic, wherein saturated means that only single bonds are present, and partially or fully unsaturated means that one or more double bonds may be present in suitable positions, while the Hückel rule for aromaticity is not fulfilled, whereas aromatic means that the Hückel (4n+2) rule is fulfilled. In principal, for being “aromatic”, it is sufficient if one of the two rings of the bicyclic moieties is aromatic, while the other is non-aromatic. However, it is preferred in connection with the term “aromatic” that both rings of the bicyclic moiety are aromatic, so that, e.g., 8 π electrons are present in case of a 9- or 10-membered aromatic heterobicyclic ring. The heterobicycle typically comprises one or more, e.g. 1, 2, 3, or 4, preferably 1, 2, or 3 heteroatoms selected from N, O and S as ring members, where S-atoms as ring members may be present as S, SO or SO₂. The remaining ring members are carbon atoms. Examples of heterobicycles include benzofuranyl, benzothienyl, indolyl, indazolyl, benzimidazolyl, benzoxathiazolyl, benzoxadiazolyl, benzothiadiazolyl, benzoxazinyl, quinolinyl, isoquinolinyl, purinyl, 1,8-naphthyridyl, pteridyl, pyrido[3,2-d]pyrimidyl, pyridoimidazolyl, triethylenediamine or quinuclidine and the like. Preferred heterobicycles according to the invention are aromatic heterobicycles such as benzodiazole, benzothiazole, quinoline, and iso-quinoline.

The term “hetaryl” or “heteroaryl” or “aromatic heterocycle” or “aromatic heterocyclic ring” includes monocyclic 5- or 6-membered aromatic heterocycles comprising as ring members 1, 2, 3 or 4 heteroatoms selected from N, O and S, where S-atoms as ring members may be present as S, SO or SO₂. Examples of 5- or 6-membered aromatic heterocycles include pyridyl (also referred to as pyridinyl), i.e. 2-, 3-, or 4-pyridyl, pyrimidinyl, i.e. 2-, 4- or 5-pyrimidinyl, pyrazinyl, pyridazinyl, i.e. 3- or 4-pyridazinyl, thienyl, i.e. 2- or 3-thienyl, furyl, i.e. 2- or 3-furyl, pyrrolyl, i.e. 2- or 3-pyrrolyl, oxazolyl, i.e. 2-, 3- or 5-oxazolyl, isoxazolyl, i.e. 3-, 4- or 5-isoxazolyl, thiazolyl, i.e. 2-, 3- or 5-thiazolyl, isothiazolyl, i.e. 3-, 4- or 5-isothiazolyl, pyrazolyl, i.e. 1-, 3-, 4- or 5-pyrazolyl, i.e. 1-, 2-, 4- or 5-imidazolyl, oxadiazolyl, e.g. 2- or 5-[1,3,4]oxadiazolyl, 4- or 5-(1,2,3-oxadiazol)yl, 3- or 5-(1,2,4-oxadiazol)yl, 2- or 5-(1,3,4-thiadiazol)yl, thiadiazolyl, e.g. 2- or 5-(1,3,4-thiadiazol)yl, 4- or 5-(1,2,3-thiadiazol)yl, 3- or 5-(1,2,4-thiadiazol)yl, triazolyl, e.g. 1H-, 2H- or 3H-1,2,3-triazol-4-yl, 2H-triazol-3-yl, 1H-, 2H-, or 4H-1,2,4-triazolyl and tetrazolyl, i.e. 1H- or 2H-tetrazolyl. Unless otherwise indicated, the term “hetaryl” further covers “aromatic heterobicycles” as defined above.

The term “aryl” or “aromatic carbocycle” preferably includes 6-membered aromatic carbocyclic rings based on carbon atoms as ring members. A preferred example is phenyl. Unless otherwise indicated, the term “aryl” further covers “aromatic carbobicycles” as defined above.

As used herein, the terms “carbocyclylalkyl” and “heterocyclylalkyl” as well as the terms “arylalkyl”, “cycloalkylalkyl”, “hetarylalkyl”, and the like refer to the corresponding groups, which are bonded to the remainder of the molecule via an alkyl, preferably via a C₁-C₂-alkyl group. Preferred examples include benzyl (i.e. phenylmethyl), cyclohexylmethyl, pyridinylmethyl, and piperidinomethyl.

As used herein, the terms “aryloxy” and “benzyloxy” refer to the corresponding groups, which are bonded to the remainder of the molecule via an oxygen atom. Preferred examples include phenyloxy and phenylmethyloxy (i.e. benzyloxy).

As used herein, the term “alkylene” refers to a linking straight-chain or branched alkylene group having usually from 1 to 4 carbon atoms, e.g. 1, 2, 3, or 4 carbon atoms. The alkylene group bridges a certain group to the remainder of the molecule. Preferred alkylene groups include methylene (CH₂), ethylene (CH₂CH₂), propylene (CH₂CH₂CH₂) and the like. A skilled person understands that, if it is referred, e.g., to CH₂ that the carbon atom being tetravalent has two valences left for forming a bridge (—CH₂—). Similarly, when it is referred, e.g., to CH₂CH₂, each carbon atom has one valence left for forming a bridge (—CH₂CH₂—). Furthermore, when it is referred, e.g., to CH₂CH₂CH₂, each terminal carbon atom has one valence left for forming a bridge (—CH₂CH₂CH₂—).

If the term “alkylene” is used in connection with, e.g. NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D) or O—(C₁-C₄-alkylene)-NR^(C)R^(D), it is to be understood that the alkylene chain bridges the NR^(C)R^(D) group to the NR^(F) group or to the oxygen atom, which are bonded to the remainder of the molecule. Similarly, if the term “alkylene” is used in connection with, e.g. NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E), it is to be understood that the alkylene chain bridges the C(═O)R^(E) group to the NR^(F) group, which is bonded to the remainder of the molecule

The term “cyclic” moiety can refer to any cyclic groups, which are present in the compounds of formula (I), and which are defined above, e.g., cycloalkyl, cycloalkenyl, carbocycle.

As used in the specification and the claims, the singular forms of “a” and “an” also include the corresponding plurals unless the context clearly dictates otherwise. The same applies for plural forms used herein, which also include the singular forms unless the context clearly dictates otherwise.

The terms “about” and “approximately” in the context of the present invention denotes an interval of accuracy that a person skilled in the art will understand to still ensure the technical effect of the feature in question. The term typically indicates a deviation from the indicated numerical value of ±10% and preferably ±5%.

It needs to be understood that the term “comprising” is not limiting. For the purposes of the present invention, the term “consisting of” is considered to be a preferred embodiment of the term “comprising of”. If hereinafter a group is defined to comprise at least a certain number of embodiments, this is also meant to encompass a group, which preferably consists of these embodiments only.

The term “pharmaceutically acceptable excipient” as used herein refers to compounds commonly comprised in pharmaceutical compositions, which are known to the skilled person. Examples of suitable excipients are exemplary listed below. Typically, a pharmaceutically acceptable excipient can be defined as being pharmaceutically inactive.

The term “treatment” is to be understood as also including the option of “prophylaxis”. Thus, whenever reference is made herein to a “treatment” or “treating”, this is to be understood as “treatment and/or prophylaxis” or “treating and/or preventing”.

Description of Pharmaceutical Compositions According to the Present Invention

A pharmaceutical composition according to the present invention may be formulated for oral, buccal, nasal, rectal, topical, transdermal or parenteral application. Preferred non-parenteral routes include mucosal (e.g., oral, vaginal, nasal, cervical, etc.) routes, of which the oral application may be preferred. Preferred parenteral routes include but, are not limited to, one or more of subcutaneous, intravenous, intra-muscular, intraarterial, intradermal, intrathecal and epidural administrations. Preferably administration is by intravenous, subcutaneous, intra-tumoral or peri-tumoral routes. Particularly preferred is systemic administration. The compound according to formula (I) should be applied in pharmaceutically effective amounts, for example in the amounts as set out herein below.

A pharmaceutical composition of the present invention may also be designated as formulation or dosage form. A compound of formula (I) may also be designated in the following as (pharmaceutically) active agent or active compound.

Pharmaceutical compositions may be solid or liquid dosage forms or may have an intermediate, e.g. gel-like character depending inter alia on the route of administration.

In general, the inventive dosage forms can comprise various pharmaceutically acceptable excipients which will be selected depending on which functionality is to be achieved for the dosage form. A “pharmaceutically acceptable excipient” in the meaning of the present invention can be any substance used for the preparation of pharmaceutical dosage forms, including coating materials, film-forming materials, fillers, disintegrating agents, release-modifying materials, carrier materials, diluents, binding agents and other adjuvants. Typical pharmaceutically acceptable excipients include substances like sucrose, mannitol, sorbitol, starch and starch derivatives, lactose, and lubricating agents such as magnesium stearate, disintegrants and buffering agents.

The term “carrier” denotes pharmaceutically acceptable organic or inorganic carrier substances with which the active ingredient is combined to facilitate the application. Suitable pharmaceutically acceptable carriers include, for instance, water, aqueous salt solutions, alcohols, oils, preferably vegetable oils, propylene glycol, polyoxyethelene sorbitans, polyethylene-polypropylene block co-polymers such as poloxamer 188 or poloxamer 407, polyethylene glycols such as polyethylene glycol 200, 300, 400, 600, etc., gelatin, lactose, amylose, magnesium stearate, surfactants, perfume oil, fatty acid monoglycerides, diglycerides and triglycerides, polyoxyethylated medium or long chain fatty acids such as ricinoleic acid, and polyoxyethylated fatty acid mono-, di, and triglycerides such as capric or caprilic acids, petroethral fatty acid esters, hydroxymethyl celluloses such as hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxypropyl acetate succinate, polyvinylpyrrolidone, crosspovidone and the like. Preferably, the compounds of the present invention are administered in a pharmaceutical composition comprising of lipids, interbilayer crosslinked multilamellar vesicles, biodegradable poly(D,L-lactic-co-glycolic acid) [PLGA]-based or poly anhydride-based nanoparticles or microparticles, nanoporous particle-supported lipid bilayers and as a conjugate with an antibody.

The pharmaceutical compositions can be sterile and, if desired, mixed with auxiliary agents, like lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, colorings, flavoring and/or aromatic substances and the like which do not deleteriously react with the active compound. It is to be understood that the term “carrier” also covers an antibody that delivers the compound of formula (I).

If liquid dosage forms are considered for the present invention, these can include pharmaceutically acceptable emulsions, solutions, suspensions and syrups containing inert diluents commonly used in the art such as water. These dosage forms may contain e.g. microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer and sweeteners/flavoring agents.

For parenteral application, particularly suitable vehicles consist of solutions, preferably oily or aqueous solutions, as well as suspensions, emulsions, or implants. Pharmaceutical formulations for parenteral administration are particularly preferred and include aqueous solutions of the compounds of formula (I) in water-soluble form. Additionally, suspensions of the compounds of formula (I) may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances, which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran.

Particularly preferred dosage forms are injectable preparations of a compound of formula (I). Thus, sterile injectable aqueous or oleaginous suspensions can for example be formulated according to the known art using suitable dispersing agents, wetting agents and/or suspending agents. A sterile injectable preparation can also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent. Among the acceptable vehicles and solvents that can be used are water and isotonic sodium chloride solution. Sterile oils are also conventionally used as solvent or suspending medium. Preferred applications for injectable preparations comprising the compounds of the present invention are intravenous, subcutaneous, intratumoral and peritumoral administration.

Compounds of formula (I) may be injected repeatedly as boluses or given via a continuous infusion. Particularly preferred is continuous infusion.

Suppositories for rectal administration of a compound of formula (I) can be prepared by e.g. mixing the compound with a suitable non-irritating excipient such as cocoa butter, synthetic triglycerides and polyethylene glycols which are solid at room temperature but liquid at rectal temperature such that they will melt in the rectum and release the compound according to formula (I) from said suppositories.

For administration by inhalation, the compounds according to the present invention may be conveniently delivered in the form of an aerosol spray from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

Oral dosage forms may be liquid or solid and include e.g. tablets, troches, pills, capsules, powders, effervescent formulations, dragees and granules. Pharmaceutical preparations for oral use can be obtained as solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone (PVP). If desired, disintegrating agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate. The oral dosage forms may be formulated to ensure an immediate release of the compound of formula (I) or a sustained release of the compound of formula (I).

A solid dosage form may comprise a film coating. For example, the inventive dosage form may be in the form of a so-called film tablet. A capsule of the invention may be a two-piece hard gelatin capsule, a two-piece hydroxypropylmethylcellulose capsule, a two-piece capsule made of vegetable or plant-based cellulose or a two-piece capsule made of polysaccharide.

The dosage form according to the invention may be formulated for topical application. Suitable pharmaceutical application forms for such an application may be a topical nasal spray, sublingual administration forms and controlled and/or sustained release skin patches. For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.

The compositions may conveniently be presented in unit dosage forms and may be prepared by any of the methods well known in the art of pharmacy. The methods can include the step of bringing the compounds into association with a carrier, which constitutes one or more accessory ingredients. In general, the compositions are prepared by uniformly and intimately bringing the compounds into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product. Liquid dose units are vials or ampoules. Solid dose units are tablets, capsules and suppositories.

As regards human patients, the compound of formula (I) may be administered to a patient in an amount of about 0.001 mg to about 5000 mg per day, preferably of about 0.01 mg to about 1000 mg per day, more preferably of about 0.05 mg to about 250 mg per day, which is the effective amount. The phrase “effective amount” means an amount of compound that, when administered to a mammal in need of such treatment, is sufficient to treat or prevent a particular disease or condition.

Furthermore, the pharmaceutical composition may also contain the compound of formula (I) as a prodrug such as an ester or amide thereof. A prodrug is any compound, which is converted under physiological conditions or by solvolysis to any of the compounds of the invention. A prodrug may be inactive prior to administration but may be converted to an active compound of the invention in vivo.

Indications, for which the compounds of the present invention may be used

The compounds according to the present invention are suitable for use in medicine. In particular, the compounds according to the present invention are suitable for use in the treatment of a disease selected from the group consisting of inflammatory diseases, allergic diseases, autoimmune diseases, infectious diseases, cancer, and pre-cancerous syndromes. Further, the compounds of formula (I) are suitable for use in immunogenic compositions and as vaccine adjuvants.

In one embodiment, the compounds according to the invention are suitable for the treatment of diseases/disorders including, but not limited to, cancer, vaccine adjuvant, infectious diseases both bacterial and viral (e.g., HIV, HBV, HCV, HPV, filoviruses (for example Ebola or Marburg), flaviviruses (for example yellow fever virus, dengue fever virus, or Japanese encephilitis virus), poxviruses, arenaviruses (for example Lassa fever virus, Lymphocytic choriomeningitis virus (LCMV), Mobala virus, Junin virus), paramyxoviruses (for example human respiratory syncythial virus, Sendai virus, mumps, Nipah), orthomyxoviruses (for example influenza virus), coronaviruses (for example SARS, SARS-COV2), rhabdoviruses (for example rabies virus, vesicular stomatitis virus), bunyaviruses (such as Bunyamwera virus, Hantaan virus, Crimean Congo virus, California encephalitis virus, Rift Valley fever virus, or sandfly fever virus).

In one embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of cancer, pre-cancerous syndromes, and infectious diseases; or for use in an immunogenic composition or as vaccine adjuvant.

In another embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of inflammatory diseases, allergic diseases, and autoimmune diseases.

In one preferred embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of cancer or pre-cancerous syndromes.

In another preferred embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of a disease selected from the group consisting of infectious diseases or for use in an immunogenic composition or as vaccine adjuvant.

In another preferred embodiment, the compound of the present invention or a pharmaceutical composition comprising the same is for use in the treatment of inflammatory diseases, allergic diseases, infectious diseases.

Of particular relevance in connection with the present invention is the treatment of cancer. Preferably, said cancer is selected from the group consisting of breast cancer, inflammatory breast cancer, ductal carcinoma, lobular carcinoma, colon cancer, pancreatic cancer, insulinomas, adenocarcinoma, ductal adenocarcinoma, adenosquamous carcinoma, acinar cell carcinoma, glucagonoma, skin cancer, melanoma, metastatic melanoma, lung cancer, small cell lung cancer, non-small cell lung cancer, squamous cell carcinoma, adenocarcinoma, large cell carcinoma, brain (gliomas), glioblastomas, astrocytomas, glioblastoma multiforme, Bannayan-Zonana syndrome, Cowden disease, Lhermitte-Duclos disease, Wilm's tumor, Ewing's sarcoma, Rhabdomyosarcoma, ependymoma, medulloblastoma, head and neck, kidney, liver, melanoma, ovarian, pancreatic, adenocarcinoma, ductal adenocarcinoma, adenosquamous carcinoma, acinar cell carcinoma, glucagonoma, insulinoma, prostate, sarcoma, osteosarcoma, giant cell tumor of bone, thyroid, lymphoblastic T cell leukemia, chronic myelogenous leukemia, chronic lymphocytic leukemia, hairy-cell leukemia, acute lymphoblastic leukemia, acute myelogenous leukemia, chronic neutrophilic leukemia, acute lymphoblastic T cell leukemia, plasmacytoma, Immunoblastic large cell leukemia, mantle cell leukemia, multiple myeloma, megakaryoblastic leukemia, multiple myeloma, acute megakaryocyte leukemia, promyelocytic leukemia, erythroleukemia, malignant lymphoma, hodgkins lymphoma, non-hodgkins lymphoma, lymphoblastic T cell lymphoma, Burkitt's lymphoma, follicular lymphoma, neuroblastoma, bladder cancer, urothelial cancer, vulval cancer, cervical cancer, endometrial cancer, renal cancer, mesothelioma, esophageal cancer, salivary gland cancer, hepatocellular cancer, gastric cancer, nasopharyngeal cancer, buccal cancer, cancer of the mouth, GIST (gastrointestinal stromal tumor), neuroendocrine cancers and testicular cancer.

More preferably, said cancer is selected from prostate cancer, renal carcinoma, melanoma, pancreatic cancer, cervical cancer, ovarian cancer, colon cancer, head and neck cancer, lung cancer, fibrosarcoma and breast cancer.

Preferably, said autoimmune disease is selected from the group consisting of systemic lupus erythematosis, Addison's disease, autoimmune polyglandular disease (also known as autoimmune polyglandular syndrome), glomerulonephritis, rheumatoid arthritis scleroderma, chronic thyroiditis, Graves' disease, autoimmune gastritis, diabetes, autoimmune hemolytic anemia, glomerulonephritis, rheumatoid arthritis autoimmune neutropenia, thrombocytopenia, atopic dermatitis, chronic active hepatitis, myasthenia gravis, multiple sclerosis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, psoriasis, graft vs. host disease, asthma, bronchitis, acute pancreatitis, chronic pancreatitis and allergies of various types.

It is to be understood that in connection with the medical uses of the invention it can be preferred that the compounds according to the present invention are administered in combination with antibodies, radiotherapy, surgical therapy, immunotherapy, chemotherapy, toxin therapy, gene therapy, or any other therapy known to those of ordinary skill in the art for treatment of a particular disease. This is particularly relevant in connection with the treatment of cancer. Preferably, the compounds of the present invention are administered in combination with antibodies. Preferred antibodies include anti-PD-1, anti-PD-L1, anti-CTLA-4, anti-IDO, anti-KIR, anti-TIM-3, anti-Vista, anti-TIGIT, anti-BTLA and anti-LAG3 antibody. Non-limiting examples are BMS-936559, MPDL3280A and MEDI4736 or avelumab (anti-PD-L1 antibodies), MK-3475, pembrolizumab or pidilizumab (anti-PD-1 antibodies) as well as ipilimumab (anti-CTLA-4 antibodies). Preferably, the compounds of the present invention are administered in a pharmaceutical composition comprising one or more of adjuvants, inactivated or attenuated bacteria (e.g., inactivated or attenuated Listeria monocytogenes), modulators of innate immune activation, preferably agonists of Toll-like Receptors (TLRs, preferably TLR7 or TLR9 agonists, e.g. SM360320, AZD8848), (NOD)-like receptors (NLRs, preferably NOD2 agonist), retinoic acid inducible gene-based (RIG)-1-like receptors (RLRs), C-type lectin receptors (CLRs), or pathogen-associated molecular patterns (“PAMPs”), cytokines (not limiting examples e.g. IL-2, IL-12, IL-6), interferons (including, but not limited to IFN alpha, IFN beta, IFN gamma, IFN lambda) or chemotherapeutic agents. The medical use may further compromise administering at least one HBV vaccine, a nucleoside HBV inhibitor or any combination thereof (e.g. RECOMBIVAX HB, ENGERIX-B, GE-NEVAC-B).

Combination therapy may be achieved by use of a single pharmaceutical composition that includes both agents, or by administering two distinct compositions at the same time, wherein one composition includes a compound of the present invention, and the other includes the second agent(s).

The two therapies may be given in either order and may precede or follow the other treatment by intervals ranging from minutes to weeks. In embodiments where the other agents are applied separately, one would generally ensure that a significant period of time did not expire between the time of each delivery, such that the agents would still be able to exert an advantageously combined effect on the patient. In such instances, it is contemplated that one may administer both modalities within about 12-24 h of each other and, more preferably, within about 6-12 h of each other. In some situations, it may be desirable to extend the time period for treatment significantly, however, where several days (2, 3, 4, 5, 6 or 7) to several weeks (1, 2, 3, 4, 5, 6, 7 or 8) lapse between the respective administrations. In some embodiments, the compound of the present invention is administered prior to administration of the distinct cancer treatment. In other embodiments, the distinct cancer treatment is administered prior to administration of the compound of the present invention.

The present invention is further illustrated by the following examples.

EXAMPLES

The following abbreviations are used herein:

Abbreviation Meaning 4 Å MS 4 Å molecular sieves ACN Acetonitrile Ac₂O Acetic anhydride anh. Anhydrous aq. Aqueous BBBPY 4,4′-di-tert-butyl-2,2′-bipyridine BINAP 2,2′-Bis(diphenylphosphino)-1,1′-binaphthalene BrettPhos 2-(Dicyclohexylphosphino)-3,6-dimethoxy-2′,4′,6′-triisopropyl-1,1′-biphenyl BTFFH Fluoro-N,N,N′,N′-bis(tetramethylene)formamidinium hexafluorophosphate t-BuXPhos 2-Di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl CH₃I Iodomethane Cs₂CO₃ Cesium carbonate CuCl₂ Copper(II) chloride CuSO₄*5H₂O Cooper sulfate pentahydrate CyPF-t-Bu (R)-1-[(SP)-2-(Dicyclohexylphosphino)ferrocenyl]ethyldi-tert-butylphosphine DBU 1,8-Diazabicyclo(5.4.0)undec-7-en DCE 1,2-Dichloroethane DCM Dichloromethane DIBAL-H Diisobutylaluminum hydride DIPEA N,N-Diisopropylethylamine DMAP 4-(Dimethylamino)pyridine DMF N,N-Dimethylformamide DMSO-d₆ Deuterated dimethylsulfoxide D₂O Deuterium Oxide EDC N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide eq. Equivalent ESI-MS Electrospray Ionisation - Mass spectrometry Et₂O Diethyl ether EtOAc Ethyl acetate EtOH Ethanol FA Formic acid FB Free base form of a compound FCC Flash column chromatography H₂ Molecular hydrogen HATU 1-[Bis(dimethylamino)methylene]-1 H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate HCl Hydrochloric acid HCOOH Formic acid Hex Hexane H₂O Water HMT 1,3,5,7-tetraazatricyclo[3.3.1.13,7]decane HOBt Hydroxybenzotriazole HPLC High-performance liquid chromatography HRMS High Resolution Mass Spectometry iPrOH Isopropyl alcohol K₂CO₃ Potassium carbonate LC-MS Liquid chromatography - mass spectrometry LDA Lithium diisopropylamide solution LiOH × Lithium hydroxide monohydrate H₂O/LiOH * H₂O MeTHF 2-Methyltetrahydrofuran Me4tButylXphos 2-Di-tert-butylphosphino-3,4,5,6-tetramethyl-2′,4′,6′-triisopropyl-1,1′-biphenyl MeCN Acetonitrile MeOD-d₄ Deuterated methanol MeOH Methanol MeONa Sodium methoxide MgSO₄ Magnesium sulfate MnO₂ Manganese(IV) oxide MPA Mercaptophenylaminobut-2-enoate ester MW Microwave irradiation, microwave heating N/M Molar concentration [mol/dm³] NaBH₄ Sodium borohydride NaBH(OAc)₃, STAB Sodium triacetoxyborohydride NaH Sodium hydride NaHCO₃ Sodium bicarbonate NaHMDC Sodium bis(trimethylsilyl)amide NaI Sodium iodide NaO t-Bu Sodium tert-butoxide NaOAc Sodium acetate NaOH Sodium hydroxide Na₂SO₄ Sodium sulfate NH₃ Ammonia NH₂ column PF-NH2, Normal Phase, Bonded Silica, NH2 NH₄OH Ammonium hydroxide NH₄Cl Ammonium chloride NMR Nuclear magnetic resonance o/n Overnight o/w over weekend Pd/C Palladium(0) on carbon Pd(dba)₂ bis((1E,4E)-1,5-diphenylpenta-1,4-dien-3-one) palladium Pd₂(dba)₃ Tris(dibenzylideneacetone)dipalladium(0) Pd(dppf)Cl₂* [1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II), complex with di- DCM chloromethane Pd(OAc)₂ Palladium II acetate Pd[P(o- Bis[tris(2-methylphenyl)phosphine]palladium Tol)₃]₂ PF-50DIOL Puriflash DIOL 50 μM flash column POCl₃ Phosphorus (V) oxychloride PTSA p-Toluenesulfonic acid prep-HPLC Preparative HPLC purification rac Racemate/racemic rac-BI- (±)-2,2′-Bis(diphenylphosphino)-1,1′-binaphthalene NAP/BINAP RP-FCC Reversed-phase flash column chromatography RM Reaction mixture RT Room temperature, i.e. 20-25° C. RuPhos Pd (2-Dicyclohexylphosphino-2′,6′-diisopropoxy-1,1′-biphenyl)[2-(2′-amino-1,1′- G3 biphenyl)]palladium(II) methanesulfonate SEM 2-(Trimethylsilyl)ethoxymethyl SPhos 2-Dicyclohexylphosphino-2′,6′-dimethoxybiphenyl SiHP Silica PuriFlash Columns High Performance C18HP C18 PuriFlash Columns High Performance ALN Alumina Neutral PuriFlash Columns TBDMS tert-Butyldimethylsilyl TBDMSCI tert-Butyldimethylsilyl chloride tBuBrettPhos di-tert-butyl[3,6-dimethoxy-2′,4′,6′-tris(propan-2-yl)-[1,1′-biphenyl]-2-yl]phos- phane tBuXPhos- [(2-Di-tert-butylphosphino-2′,4′,6′-triisopropyl-1,1′-biphenyl)-2-(2′amino-1,1-bi- Pd-G3 phenyl)] palladium(II) methanesulfonate TEA Triethylamine TFA Trifluoroacetic acid THF Tetrahydrofurane UPLC Ultra performance liquid chromatography UPLC-MS Ultra performance liquid chromatography-mass spectometry Xantphos (9,9-Dimethyl-9H-xanthene-4,5-diyl)bis(diphenylphosphine)

The compounds of the present invention were prepared according to the procedures of the following Schemes and Examples, using appropriate materials and are further exemplified by the following specific examples.

Unless otherwise specified, all starting materials are obtained from commercial suppliers and used without further purifications. Unless otherwise specified, all temperatures are expressed in ° C. and all reactions are conducted at RT.

Methods and Analytical Data: General:

Microwave heating (MW) was done using Anton Paar Monowave 450 or Biotage Emrys Initiator microwave reactor. Column chromatography was carried out using the Isco Rf200d or the Interchim PuriFlash 450. Solvent removal was carried out using either a Büchi rotary evaporator or a Genevac centrifugal evaporator. Analytical LC-MS was conducted using a Waters I Class SQD2, column X Bridge 1.7 um 2.1×50 mm under acidic conditions. Preparative HPLC was conducted using Waters auto-purification system or Shimadzu Preparative H PLC system, column 19×150 mm XSelect 5 micron C18 column under basic mobile phase conditions or Phenomenex Gemini NX 21.2×250 mm C18 column under acidic conditions. NMR spectra were recorded using a Bruker 300 MHz or 400 MHz spectrometer. Chemical shifts (δ) are reported in ppm relative to the residual solvent signal (measurement range—6.4 kHz). ¹H NMR data are reported as follows: chemical shift (multiplicity, coupling constants and number of hydrogens). Multiplicity is abbreviated as follows: s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), dd (doublet of doublets), dt (doublet of triplets), ddd (doublet of doublet of doublets). ESI-MS: Desolvatation Gas Flow 993 l/h; Desolvatation temperature 500° C.; cone gas: 50 l/min; 500-1000 m/z; polarity: positive and/or negative.

Preparative HPLC Conditions for the Purification of Target Compounds: Chromatography Conditions 1:

Prep HPLC Instrument: Shimadzu or Auto-purification System Waters

Column: Gemini-NX 5 μm C18 110 Å, 21.2*250 mm

Detector: SPD-20 Å/20AV UV-VIS

Flow Rate: 20 mL/min

Representative Mobile Phase:

(1)

Mobile Phase: A: 0.1% formic acid (FA) or trifluoroacetic acid (TFA) in water

Mobile Phase: B: 0.1% FA or TFA in MeCN

(2)

Mobile Phase: A: 0.1% NH₄OH in water

Mobile Phase: B: 0.1% NH₄OH in MeCN

Chromatography Conditions 2:

Prep HPLC Instrument: Shimadzu or Auto-purification System Waters

Column: Chiralpak AD-H, 5 μm, 20*250 mm or IF, 5 μm, 20*200 mm

Detector: SPD-20A/20AV UV-VIS

Flow Rate: 20 mL/min

Representative Mobile Phase:

Mobile Phase: A: EtOH

Mobile Phase: B: hexane

UPLC, HPLC and MS data provided in the examples described below were registered on:

LC-MS Analyses on Waters:

Method name: lc-ms1-2-ba

Equipment:

-   -   Waters I Class SQD2     -   UPLC with DAD detector     -   column: Waters Acquity UPLC X Bridge C18, 50 mm×2.1 mm×1.7 μm

Eluents:

(A) 0.1% formic acid in water

(B) 0.1% formic acid in MeCN

Analytical method:

-   -   Autosampler: injection volume: 1 μL     -   Pump:

Time [min] Flow [mL/min] % B 0.00 0.5 1 0.10 0.5 1 1.10 0.5 100 2.00 0.5 100 2.50 0.5 1 3.00 0.5 1

-   -   Column compartment: column temperature: 40° C.; time of         analysis: 3 min     -   Detector: wave length: 254, 214, 280 nm

LC-MS Analyses on Bruker Amazon SL

Method name: lc-ms1-2-ba

Equipment:

-   -   MS Bruker Amazon SL     -   LC Dionex Ultimate 3000     -   HPLC with UV-Vis or DAD detector     -   column: Waters Acquity UPLC HSS C18, 50 mm×2.1 mm×1.8 μm

Eluents:

(A) 0.1% formic acid in MeCN

(B) 0.1% formic acid in water

Analytical method:

-   -   Auto sampler: injection volume: 1 μL     -   Pump:

Time [min] Flow [mL/min] % B 0.00 0.5 95 0.00 0.5 95 4.00 0.5 5 5.00 0.5 5 5.20 0.5 95 6.00 0.5 95

-   -   Column compartment: column temperature: 25° C.; time of         analysis: 6 min     -   Detector: wave length: 254, 230, 270, 280 nm

LC-MS Analyses on Bruker Amazon SL

Method name: BCM-30

Equipment:

-   -   MS Bruker Amazon SL     -   LC Dionex Ultimate 3000     -   HPLC with UV-Vis or DAD detector     -   column: Waters Symmetry C18 3.9×150 mm 5 μm

Eluents:

(A) 0.1% formic acid in water

(B) 0.1% formic acid in MeCN

Analytical method:

-   -   Autosampler: injection volume: 3 μL     -   Pump:

flow: 1.2 mL/min

Time [min] [%] B 0.0 20 20.0 80 22.0 80 22.5 95 25.0 95 25.3 20 30.0 20

-   -   Column compartment: column temperature: 25° C.; time of         analysis: 30 min     -   Detector: wave length: 254 nm

LC-MS Analyses on Corona Ultra:

Method name: BCM-30

Equipment:

-   -   Corona ultra     -   LC Dionex Ultimate 3000     -   column: Waters Symmetry C18 3.9×150 mm 5 μm

Eluents:

(A) 0.1% formic acid in water

(B) 0.1% formic acid in MeCN

Analytical method:

-   -   Autosampler: injection volume: 3 μL     -   Pump:

flow: 1.2 mL/min

Time [min] [%] B 0.0 20 20.0 80 22.0 80 22.5 95 25.0 95 25.3 20 30.0 20

Synthetic Procedures:

The following compounds are commercially available and/or can be prepared in a number of ways well known to one skilled in the art of organic synthesis. More specifically, disclosed compounds can be prepared using the reactions and techniques described herein. In the description of the synthetic methods described below, it is to be understood that all proposed reaction conditions, including choice of solvent, reaction atmosphere, reaction temperature, duration of the experiment, and workup procedures, can be chosen to be the conditions standard for that reaction, unless otherwise indicated. It is understood by one skilled in the art of organic synthesis that the functionality present on various portions of the molecule should be compatible with the reagents and reactions proposed. Substituents not compatible with the reaction conditions will be apparent to one skilled in the art, and alternate methods are therefore indicated. The starting materials for the examples are either commercially available or are readily prepared by standard methods from known materials.

PREPARATION OF EXAMPLES Example 1. 3-({[(3S)-1-(3-Aminophenyl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1)

To anh. DMF (5.0 mL), POCl₃ (3.10 mL, 6.0 eq.) was added dropwise at 0° C. and the mixture was stirred at RT for 15 min. Then 1-(2-aminophenyl)ethan-1-one (0.75 g, 1.0 eq.) in anh. DMF (3.0 mL) was added dropwise and the reaction was heated at 60° C. for 3 h. Afterwards, the reaction was cooled down and quenched with water. Then the mixture was neutralized with aq. solution of NaHCO₃ and extracted with DCM. The organic layer was dried over anh. MgSO₄ and the solvent was removed in vacuo to give a crude brown solid (0.33 g). 0.15 g of the crude residue was suspended in 54% aq. solution of HCOOH (1.83 mL) and the reaction was stirred at 50° C. for 2 h and subsequently kept at 4° C. overnight. A precipitate formed, which was filtered off, washed with water, and triturated with diethyl ether to give the product (0.135 g) as an orange solid. ESI-MS: 174.0 [M+H]⁺.

Preparation of 1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

4-Oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1) (0.50 g, 1.0 eq.) was suspended in THF (13.0 mL). DBU (1.1 g, 2.5 eq.) was added followed by methyl iodide (4.1 g, 10.0 eq.) and the reaction was stirred at 40° C. overnight. Additional portions of DBU and methyl iodide were added and the reaction was continued overnight. Afterwards, the reaction was quenched with water and extracted with DCM. Organic layer was dried over anh. Na₂SO₄ and solvent was removed in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to provide the product (0.350 g, yield 63%) as a yellow solid. ESI-MS: 188.1 [M+H]⁺.

Preparation of tert-butyl (3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl]amino}piperidine-1-carboxylate

A mixture of (S)-1-tert-butoxycarbonyl-3-aminopiperidine (4.17 g, 1.3 eq.), 1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (3.0 g, 1.0 eq.), NaOAc (1.32 g, 1.0 eq.) and 4 Å MS (0.7 g) in anh. MeOH (100.0 mL) was stirred under inert atmosphere at RT overnight. After that the mixture was cooled to 0° C. and NaBH₄ (0.67 g, 1.1 eq.) was added portionwise over 1 h. The reaction was continued at RT for 3 h. The mixture was filtered through a pad of Celite, the filter cake was washed with methanol and the filtrate was concentrated in vacuo. The residue was diluted with DCM and washed with 10% aq. solution of NaOH, brine, dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (ALN; DCM:MeOH) to afford the product (5.60 g, yield 94%) as a yellow solid. ESI-MS: 372.2 [M+H]⁺.

Preparation of tert-butyl (3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate

A mixture of tertbutyl (3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl]amino}piperidine-1-carboxylate (0.550 g, 1.0 eq.), 2-methyl-4-pyridinecarboxaldehyde (0.18 g, 1.0 eq.) and 4 Å MS in a mixture of anh. DCE and DMF (20.0 mL, 1:1) was stirred at RT overnight, then cooled to 0° C. and NaBH(OAc)₃ (0.47 g, 1.5 eq.) was added portionwise. The mixture was stirred at 45° C. for 3 h, then filtered through a pad of Celite pad, washed with DCM and concentrated in vacuo. The residue was diluted with DCM and washed with 10% aq. solution of NaOH, brine, dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.61 g, yield 86%) as a pale yellow solid. ESI-MS: 477.2 [M+H]⁺.

Preparation of 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5)

To a solution of tert-butyl (3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate (0.60 g, 1.0 eq.) in 1,4-dioxane (10 mL) 4M HCl in 1,4-dioxane (1.57 mL, 5.0 eq.) was added. The resulting mixture was stirred at RT overnight and subsequently poured into water and basified with 1M aq. solution of NaOH. The mixture was extracted with DCM, combined organic layers were washed with brine and dried over anh. Na₂SO₄. The solvent was removed under reduced pressure to afford the product (0.375 g, yield 79%) as a white solid. ESI-MS: 377.5 [M+H]⁺.

Preparation of tert-butyl N-{3-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]phenyl}carbamate

1-Methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.150 g, 1.0 eq.), tert-butyl N-(3-bromophenyl)carbamate (0.108 g, 1.0 eq.) and NaO t-Bu (0.077 g, 2.0 eq.) were suspended in anh. 1,4-dioxane and argon was bubbled through the mixture. Under an inert atmosphere RuPhos Pd G3 (0.033 g, 0.1 eq.) was added. The reaction was carried out at 100° C. overnight. Subsequently, the mixture was cooled to ambient temperature and filtered through a pad of Celite. The filter cake was washed with AcOEt and the filtrate was concentrated under reduced pressure. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.162 g, yield 72%) as a yellow oil. ESI-MS: 568.7 [M+H]⁺.

Preparation of 3-({[(3S)-1-(3-aminophenyl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

To a solution of tert-butyl N-{3-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]phenyl}carbamate (0.162 g, 1.0 eq.) in 1,4-dioxane (3.0 mL) 4M HCl in 1,4-dioxane was added (0.438 mL, 5.0 eq.). The mixture was stirred at RT for 3 days and subsequently concentrated in vacuo. The residue was diluted with DCM and washed with 15% aq. solution of NaOH. The organic layer was washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The mixture was separated by prep-HPLC (MeOH:H₂O; FA). Obtained solid was partitioned between DCM and saturated aq. solution of NaHCO₃. Organic layer was dried over anh. Na₂SO₄, filtered and evaporated. The residue was repurified by prep-HPLC (MeOH:H₂O; NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.045 mL, 3.0 eq.) and DCM as a solvent (3.0 mL) to provide the product (0.017 g, yield 15%) as a yellow solid. ESI-MS: 468.3 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.16 (d, J=6.2 Hz, 1H), 8.11 (s, 1H), 8.05 (dd, J=8.3, 1.5 Hz, 1H), 7.84 (ddd, J=8.7, 7.1, 1.6 Hz, 1H), 7.68 (d, J=8.7 Hz, 1H), 7.62 (d, J=6.3 Hz, 1H), 7.58 (s, 1H), 7.52 (t, J=7.6 Hz, 1H), 7.35 (t, J=8.2 Hz, 1H), 7.07 (dd, J=8.5, 1.8 Hz, 2H), 7.01-6.98 (m, 1H), 6.93 (dd, J=7.9, 2.0 Hz, 1H), 4.62-4.53 (m, 3H), 4.31 (d, J=13.5 Hz, 1H), 3.88 (s, 3H), 3.85-3.79 (m, 1H), 3.71-3.65 (m, 1H), 3.63-3.56 (m, 1H), 3.39-3.26 (m, 2H), 2.32 (s, 3H), 2.25-2.10 (m, 3H), 1.87 (s, 1H).

Example 2. 3-({[(3S)-1-(6-Aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

Preparation of 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

Reaction vessel was charged with 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.10 g, 1.0 eq.), 5-bromo-2-nitropyridine (0.108 g, 2.0 eq.), NaO t-Bu (0.051 g, 2.0 eq.) and RuPhos Pd G3 (0.022 g, 0.1 eq.). Air was removed and the vessel was backfilled with argon. Anh. 1,4-dioxane (5.0 mL) was added and the mixture was heated at 100° C. overnight. After that the solvent was removed in vacuo and the residue was separated by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to afford the product (0.08 g, yield 60%) as a yellow solid. ESI-MS: 499.2 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.33-8.27 (m, 1H), 8.25 (d, J=3.0 Hz, 1H), 8.19 (dd, J=8.1, 1.6 Hz, 1H), 8.08 (d, J=9.2 Hz, 1H), 8.03 (s, 1H), 7.71 (ddd, J=8.5, 6.8, 1.6 Hz, 1H), 7.62 (d, J=8.5 Hz, 1H), 7.44 (dd, J=9.4, 3.1 Hz, 1H), 7.37 (ddd, J=8.0, 6.8, 1.1 Hz, 1H), 7.30-7.19 (m, 2H), 4.37-4.19 (m, 1H), 4.11-3.95 (m, 1H), 3.86 (s, 3H), 3.83-3.71 (m, 2H), 3.71-3.56 (m, 2H), 3.24-3.12 (m, 1H), 3.05-2.90 (m, 1H), 2.70-2.57 (m, 1H), 2.38 (s, 3H), 2.07-1.93 (m, 1H), 1.90-1.61 (m, 2H), 1.53-1.28 (m, 1H).

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

To a solution of 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.025 g, 0.050 mmol, 1.0 eq.) in ethanol (5.0 mL), Pd/C (10 wt. %, 0.001 g, 0.1 eq.) was added. The mixture was stirred overnight under hydrogen atmosphere. After filtration and solvent evaporation, the residue was purified using RP-FCC (C18HP; H₂O:MeCN) to afford the product (0.014 g, yield 85%) as a yellow solid. ESI-MS: 469.3 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.33-8.24 (m, 1H), 8.19 (dd, J=8.1, 1.6 Hz, 1H), 7.99 (s, 1H), 7.72 (ddd, J=8.6, 6.8, 1.6 Hz, 1H), 7.66-7.55 (m, 2H), 7.37 (ddd, J=8.0, 6.8, 1.1 Hz, 1H), 7.28-7.18 (m, 2H), 7.14 (dd, J=8.9, 2.9 Hz, 1H), 6.37 (d, J=8.8 Hz, 1H), 5.37 (s, 2H), 3.84 (s, 3H), 3.78-3.54 (m, 4H), 3.54-3.46 (m, 1H), 3.28-3.17 (m, 1H), 2.83-2.73 (m, 1H), 2.63-2.54 (m, 1H), 2.43 (d, J=12.6 Hz, 1H), 2.36 (s, 3H), 2.05-1.90 (m, 1H), 1.82-1.69 (m, 1H), 1.58-1.32 (m, 2H).

Example 3. 3-({[(3S)-1-[6-(Aminomethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-(5-bromopyridin-2-yl)methyl)carbamate

To a solution of 5-bromopyridine-2-carbonitrile (1.0 g, 1.0 eq.) in methanol (10.0 mL) NiCl₂*6H₂O (0.13 g, 0.1 eq.) and di-tert-butyl dicarbonate (2.39 g, 2.0 eq.) were added. The mixture was cooled to 0° C. and NaBH₄ (0.41 g, 2.0 eq) was added. The resulting reaction mixture was stirred at RT for 24 h. Subsequently, solvent was evaporated in vacuo and the residue was diluted with water and extracted with AcOEt. The organic layer was dried over anh. Na₂SO₄ and solvent was evaporated in vacuo. The residue was purified by FCC (SiHP; Hex:AcOEt) and repurified by RP-FCC (C18HP; MeOH:H₂O) to provide the product (0.19 g, yield 12%) as a yellow oil. ESI-MS: 287.1 [M+H]⁺

Preparation of tert-butyl N-({5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}methyl)carbamate

1-Methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.16 g, 1.0 eq.), tert-butyl N-(5-bromopyridin-2-yl)methyl)carbamate (0.134 g, 1.1 eq.) and Cs₂CO₃ (0.28 g, 2.0 eq.) were suspended in anh. 1,4-dioxane and argon was bubbled through the reaction mixture for 5 minutes. Under inert atmosphere Pd₂(dba)₃ (0.078 g, 0.2 eq.) and Xantphos (0.074 g, 0.3 eq.) were added and the reaction was stirred at 115° C. overnight. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of Celite and concentrated under reduced pressure. The residue was combined with a parallel reaction performed in the same manner starting from 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.05 g,), tert-butyl N-(5-bromopyridin-2-yl)methyl)carbamate (0.05 g, 1.3 eq.), Cs₂CO₃ (0.087 g, 2.0 eq.), Pd₂(dba)₃ (0.024 g, 0.2 eq.) and Xantphos (0.023 g, 0.3 eq.). The combined residues were purified by FCC (SiHP; DCM:MeOH) to afford the product (0.158 g, combined yield 64%) as a yellow solid. ESI-MS: 583.8 [M+H]⁺.

Preparation of 3-({[(3S)-1-[6-(aminomethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

To a solution of tert-butyl N-({5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}methyl)carbamate (0.158 g, 1.0 eq.) in 1,4-dioxane (4.2 mL) 4M HCl in 1,4-dioxane (5.0 mL) was added. The mixture was stirred at 55° C. for 1 h and subsequently concentrated in vacuo. The residue was diluted with DCM and washed with aq. solution of NaOH. The organic layer was dried over Na₂SO₄ and concentrated in vacuo. The residue was purified by prep-HPLC (MeOH:H₂O; NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.08 mL, 2.0 eq.) and DCM as a solvent (5.0 mL) to provide the product (0.046 g, yield 31%) as a yellow solid. ESI-MS: 483.2 [M+H]⁺. ¹H NMR (400 MHz, D₂O) δ 8.27 (d, J=6.1 Hz, 1H), 8.25 (d, J=2.9 Hz, 1H), 8.12 (s, 1H), 8.05 (dd, J=8.3, 1.5 Hz, 1H), 7.86 (ddd, J=8.6, 7.1, 1.6 Hz, 1H), 7.80-7.74 (m, 2H), 7.74-7.66 (m, 2H), 7.57-7.50 (m, 2H), 4.79-4.64 (m, 2H+H₂O), 4.62 (d, J=13.7 Hz, 1H), 4.45 (d, J=13.8 Hz, 1H), 4.24 (d, J=3.2 Hz, 2H), 4.00 (d, J=12.8 Hz, 1H), 3.88 (s, 4H), 3.61 (dd, J=12.8, 8.4 Hz, 1H), 3.58-3.50 (m, 1H), 3.29-3.18 (m, 1H), 2.39 (s, 3H), 2.35-2.27 (m, 1H), 2.26-2.16 (m, 1H), 2.14-2.05 (m, 1H), 1.88-1.77 (m, 1H).

Example 4. 3-({[(3S)-1-(6-Aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]carbamate

To a solution of 5-bromo-2-nitropyridine (2.25 mL, 1.0 eq.) in anh. 1,4-dioxane (96.0 mL) tert-butyl N-[(3S)-piperidin-3-yl]carbamate (4.62 g, 1.3 eq.) and Cs₂CO₃ (7.8 g, 1.35 eq.) were added and argon was bubbled through the mixture for 5 minutes. Then Pd₂(dba)₃ (0.81 g, 0.05 eq.) and Xantphos (0.62 g, 0.06 eq.) were added and the resulting mixture was heated at 115° C. under an inert atmosphere for 2 days. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of Celite and concentrated under reduced pressure. The residue was re-dissolved in DCM, stirred overnight with MPA metal scavenger and purified by FCC (SiHP; Hex:EtOAc) to give the product (3.6 g, yield 63%) as a pale yellow oil. ESI-MS: 323.1 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.21 (d, J=3.0 Hz, 1H), 8.14 (d, J=9.3 Hz, 1H), 7.42 (dd, J=9.3, 3.1 Hz, 1H), 7.03 (d, J=7.1 Hz, 1H), 3.99-3.76 (m, 2H), 3.48-3.37 (m, 1H), 3.21-2.97 (m, 2H), 1.94-1.70 (m, 2H), 1.56-1.46 (m, 2H), 1.41 (s, 9H).

Preparation of (3S)-1-(6-nitropyridin-3-yl)piperidin-3-amine

To a stirred solution of tert-butyl N-[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]carbamate (3.50 g, 1.0 eq.) in 1,4-dioxane (25.0 mL) 4M HCl in 1,4-dioxane (23.0 mL, 30.0 eq.) was added. The resulting mixture was stirred at 55° C. for 45 minutes and subsequently concentrated in vacuo. The residue was diluted with DCM, washed with 10% aq. solution of NaOH and brine, dried over anh. Na₂SO₄ and filtered. Solvents were removed under reduced pressure to provide the product (2.40 g, yield 99%) as a yellow solid which was taken to the next step without an additional purification. ESI-MS: 223.3 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.22 (d, J=3.1 Hz, 1H), 8.12 (d, J=9.3 Hz, 1H), 7.42 (dd, J=9.3, 3.1 Hz, 1H), 4.00-3.80 (m, 2H), 3.02 (ddd, J=13.7, 11.0, 3.1 Hz, 1H), 2.86-2.64 (m, 2H), 1.95-1.82 (m, 1H), 1.82-1.70 (m, 1H), 1.69-1.52 (m, 2H), 1.52-1.40 (m, 1H), 1.35-1.20 (m, 1H).

Preparation of 1-cyclopropyl-6,7-difluoro-1,2,3,4-tetrahydroquinolin-4-one

To a solution of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (9.0 g, 1.0 eq.) in anh. MeOH (150.0 mL) under inert atmosphere NaBH₄ (5.4 g, 4.5 eq.) was added portionwise over 30 minutes. The mixture was allowed to reach RT, p-toluenesulfonic acid monohydrate (0.61 g, 0.10 eq.) was added and the reaction mixture was heated at reflux for 3.5 h. Subsequently, the mixture was allowed to reach RT and the solvent was removed in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to provide the product (5.73 g, yield 80%) as a yellow solid. ESI-MS: 224.1 [M+H]⁺. 1H NMR (400 MHz, DMSO-d₆) δ 7.60 (dd, J=10.8, 9.3 Hz, 1H), 7.21 (dd, J=13.6, 6.7 Hz, 1H), 3.52 (dd, J=7.5, 6.4 Hz, 2H), 2.59 (dd, J=7.5, 6.3 Hz, 2H), 2.45-2.38 (m, 1H), 0.95-0.89 (m, 2H), 0.71-0.66 (m, 2H).

Preparation of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)

To a mixture of NaOMe (5.41 g, 3.9 eq.) and ethyl formate (8.14 mL, 3.9 eq.) in anh. DCM (150.0 mL) the solution of 1-cyclopropyl-6,7-difluoro-1,2,3,4-tetrahydroquinolin-4-one (5.73 g, 1.0 eq.) in anh. DCM (5.0 mL) was added under inert atmosphere and the mixture was stirred at RT overnight. Subsequently, the reaction was quenched with ice-cold water. The organic layer was washed with 3M aq. solution of NaOH. Combined aq. phases were acidified to pH=6 and extracted with DCM. Organic layers were combined, dried over anh. MgSO₄ and concentrated under reduced pressure. The residue was diluted with anh. MeOH (150.0 mL) and MnO₂ (8.44 g, 5.0 eq.) was added. After stirring at RT for 48 h, the mixture was filtered through a pad of Celite and the filter cake was washed with a mixture of DCM and MeOH (1:1). The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH). Solvents were evaporated and the residue was re-dissolved in a small volume of DCM and hexane was added. The precipitate was filtered and dried to provide the product (3.95 g, yield 62%) as a white solid. ESI-MS: 250.2 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.11 (s, 1H), 8.41 (s, 1H), 8.22 (dd, J=12.1, 6.7 Hz, 1H), 8.14 (dd, J=10.5, 8.8 Hz, 1H), 3.73-3.64 (m, 1H), 1.32-1.24 (m, 2H), 1.18-1.12 (m, 2H).

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.40 g, 1.0 eq), (3S)-1-(6-nitropyridin-3-yl)piperidin-3-amine (0.39 g, 1.1 eq.), and NaOAc (0.145 g, 1.1 eq.) in anh. MeOH (50.0 mL) was stirred under inert atmosphere at 50° C. for 30 minutes. DCM (9.0 mL) was added, the mixture was sonicated until clear and stirred at RT overnight. Then, the mixture was cooled to 0° C. and sodium borohydride (0.067 g, 1.1 eq.) was added portionwise. The mixture was allowed to reach RT and stirred for 1.5 h followed by stirring at 30° C. for 1.5 h. Subsequently, the mixture was concentrated in vacuo. The residue was partitioned between DCM and 2M aq. solution of NaOH. Organic layer was washed with brine, dried over anh. Na₂SO₄ and filtered. Solvents were removed under reduced pressure and the residue was purified by FCC (SiHP, DCM:MeOH) to provide the product (0.62 g, yield 85%) as a yellow solid. ESI-MS: 456.2 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.23 (d, J=3.1 Hz, 1H), 8.09 (d, J=9.3 Hz, 1H), 8.05-7.99 (m, 3H), 7.44 (dd, J=9.4, 3.1 Hz, 1H), 3.99 (dd, J=13.3, 3.5 Hz, 1H), 3.85-3.78 (m, 1H), 3.70-3.59 (m, 2H), 3.51 (tt, J=7.2, 3.9 Hz, 1H), 3.23-3.14 (m, 1H), 3.02 (dd, J=13.2, 8.6 Hz, 1H), 2.63-2.56 (m, 1H), 2.15 (s, 1H), 1.93-1.85 (m, 1H), 1.82-1.73 (m, 1H), 1.54-1.35 (m, 2H), 1.27-1.19 (m, 2H), 1.06-0.96 (m, 2H).

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.62 g, 1.0 eq.) and 2-methoxypyridine-4-carbaldehyde (0.16 mL, 1.0 eq.) in anh. DCE (20.0 mL) was stirred under inert atmosphere at RT overnight. Then, the mixture was cooled to 0° C. and NaBH(OAc)₃ (0.433 g, 1.5 eq.) was added portionwise. The reaction was stirred at 40° C. for 3 h. Additional portions of 2-methoxypyridine-4-carbaldehyde and NaBH(OAc)₃ was added to the reaction mixture and stirring at 50° C. was continued until no further progress of reaction was observed. Subsequently DCM and 2 M aq. solution of NaOH were added. Organic layer was washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to afford the title compound (0.545 g, yield 69%) as a yellow solid. ESI-MS: 577.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.23 (d, J=3.0 Hz, 1H), 8.08 (d, J=9.3 Hz, 1H), 8.04-7.95 (m, 3H), 7.91 (s, 1H), 7.44 (dd, J=9.3, 3.1 Hz, 1H), 6.96 (dd, J=5.3, 1.3 Hz, 1H), 6.78 (s, 1H), 4.24-4.18 (m, 1H), 4.06-3.99 (m, 1H), 3.78 (s, 2H), 3.76 (s, 3H), 3.65 (s, 2H), 3.51-3.45 (m, 1H), 3.20-3.13 (m, 1H), 3.01-2.92 (m, 1H), 2.73-2.65 (m, 1H), 2.00-1.93 (m, 1H), 1.84-1.75 (m, 1H), 1.74-1.65 (m, 1H), 1.52-1.39 (m, 1H), 1.26-1.18 (m, 2H), 0.96-0.85 (m, 2H).

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one Hydrochloride

To a solution of 1-cyclopropyl-6,7-difluoro-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.50 g, 1.0 eq) in MeOH (50.0 mL), Pd/C (10 wt. %, 0.04 g, 0.45 eq.) was added and the mixture was stirred overnight under hydrogen atmosphere. Subsequently, the reaction mixture was filtered through a pad of Celite, and the filtrate was stirred with MPA scavenger for 30 minutes. After filtration and solvent evaporation, the residue was purified using FCC (SiHP; DCM:MeOH) and re-purified by FCC (ALN; DCM:MeOH), RP-FCC (C18HP; H₂O:MeCN), and FCC (SiHP; DCM:MeOH). The compound was converted to HCl salt using 2M HCl in Et₂O (0.064 mL, 1.0 eq.) and a mixture of MeOH (5.0 mL) and H₂O (1.0 mL) as a solvent to provide the product (0.078 g, yield 15%) as a yellow solid. ESI-MS: 547.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.06 (dd, J=10.9, 8.8 Hz, 1H), 7.99 (s, 1H), 7.95 (dd, J=12.1, 6.7 Hz, 1H), 7.86 (d, J=5.3 Hz, 1H), 7.76 (dd, J=9.5, 2.8 Hz, 1H), 7.38 (d, J=2.8 Hz, 1H), 6.90 (dd, J=5.4, 1.4 Hz, 1H), 6.84 (d, J=9.4 Hz, 1H), 6.74 (s, 1H), 3.86-3.71 (m, 7H), 3.70-3.64 (m, 1H), 3.51-3.44 (m, 1H), 3.40-3.35 (m, 1H), 3.02-2.92 (m, 1H), 2.78-2.70 (m, 1H), 2.63-2.54 (m, 1H), 2.14-2.06 (m, 1H), 1.96-1.89 (m, 1H), 1.75-1.55 (m, 2H), 1.33-1.26 (m, 2H), 0.99-0.93 (m, 2H).

Example 5. 3-({[(2-Aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]carbamate

Through a mixture of 5-bromo-2-methylpyridine (3.0 g, 1.0 eq.) in anh. 1,4-dioxane (80.0 mL) tert-butyl N-[(3S)-piperidin-3-yl]carbamate (4.54 g, 1.3 eq.) and Cs₂CO₃ (7.7 g, 1.4 eq.) argon was bubbled for 5 minutes. Then Pd₂(dba)₃ (0.80 g, 0.05 eq.), and Xantphos (0.60 g, 0.06 eq.) were added and the resulting mixture was heated at 115° C. under inert atmosphere for 5 days. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of Celite and concentrated under reduced pressure. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (3.90 g, yield 77%) as a pale yellow oil. ESI-MS: 292.1 [M+H]⁺.

Preparation of (33)-1-(6-methylpyridin-3-yl)piperidin-3-amine (Intermediate 6)

To a stirred solution of tert-butyl N-[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]carbamate (0.53 g, 1.0 eq.) in 1,4-dioxane (3.0 mL) 4M HCl in 1,4-dioxane (2.73 mL, 30.0 eq.) was added and the reaction mixture was stirred at 55° C. for 1 h. After concentration under reduced pressure, the residue was diluted with DCM and washed with 10% aq. solution of NaOH. Organic layer was dried over anh. Na₂SO₄, filtered and solvents were removed under reduced pressure to provide the product (0.34 g, yield 98%) as a yellow oil which was taken to the next step without additional purification. ESI-MS: 192.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.20 g, 1.0 eq.), (3S)-1-(6-methylpyridin-3-yl)piperidin-3-amine (Intermediate 6) (0.16 g, 1.05 eq.), NaOAc (0.066 g, 1.0 eq.) and MS 4 Å (0.3 g) in a mixture of anh. MeOH and DCM (1:1, 7.0 mL) was stirred under inert atmosphere at RT overnight. Then, the mixture was cooled to 0° C., sodium borohydride (0.033 g, 1.1 eq.) was added portionwise and the mixture was stirred at RT for 1 h. Subsequently, the mixture was filtered through a pad of Celite, washed with MeOH and concentrated in vacuo. The residue was partitioned between DCM and 10% aq. solution of NaOH. Aqueous layer was washed with DCM and the combined organic phases were washed with brine and dried over anh. Na₂SO₄. Solvents were removed under reduced pressure to provide the crude product (0.315 g, yield 93%) as a yellow oil which was taken to the next step without additional purification 425.5 [M+H]⁺.

Preparation of 3-({[(2-aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.315 g, 1.0 eq.) and tert-butyl N-(4-formylpyridine-2-yl)carbamate (0.215 g, 1.3 eq.) in anh. DCE (7.0 mL) was stirred at RT overnight in presence of MgSO₄. Then NaBH(OAc)₃ (0.236 g, 1.5 eq.) was added and the reaction was stirred at 55° C. for 3 h and subsequently concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and the product was then dissolved in 1,4-dioxane (3.0 mL) and 4M HCl in 1,4-dioxane (0.6 mL) was added. The mixture was stirred at 55° C. for 1 h and then concentrated in vacuo. The residue was diluted with DCM and washed with 5% aq. solution of NaOH. Organic phase was dried over anh. Na₂SO₄, filtered, concentrated in vacuo and purified by prep-HPLC (H₂O:MeCN; NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.04 mL, 2.0 eq.) and DCM as a solvent (5.0 mL) to provide the product (0.023 g, yield 5%) as a yellow solid. ESI-MS: 531.2 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.17 (s, 1H), 8.09-8.01 (m, 2H), 7.99-7.91 (m, 2H), 7.56 (d, J=9.1 Hz, 1H), 7.44 (d, J=6.7 Hz, 1H), 6.87 (s, 1H), 6.75 (dd, J=6.7, 1.7 Hz, 1H), 4.46-4.26 (m, 4H), 4.05-3.97 (m, 1H), 3.70-3.62 (m, 1H), 3.60-3.53 (m, 1H), 3.53-3.46 (m, 1H), 3.46-3.38 (m, 1H), 3.12-3.03 (m, 1H), 2.54 (s, 3H), 2.31-2.22 (m, 1H), 2.09-1.98 (m, 2H), 1.82-1.71 (m, 1H), 1.31-1.25 (m, 2H), 0.99-0.92 (m, 2H).

Example 6. 7-(4-Aminopiperidin-1-yl)-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 7-bromo-1-methyl-1,4-dihydroquinolin-4-one

7-Bromo-4-quinolinol (3.0 g, 1.0 eq.) was dissolved in anh. DMF (6.0 mL), K₂CO₃ was added (4.45 g, 2.0 eq.). The mixture was stirred at RT for 0.5 h, then methyl iodide (1.25 mL, 1.5 eq.) was added and the mixture was stirred at RT overnight. The reaction mixture was diluted with DCM, washed with water, brine and dried over anh. Na₂SO₄. Subsequently solvents were removed under reduced pressure to afford the product (2.57 g, yield 79%) as a yellow solid used in the next step without further purification. ESI-MS: 237.9 [M+H]⁺.

Preparation of 7-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

7-Bromo-1-methyl-1,4-dihydroquinolin-4-one (2.50 g, 1.0 eq.), HMT (2.94 g, 2.0 eq.) and TFA (10.0 mL) were irradiated in microwave at 120° C. for 0.5 h. After that the mixture was heated in a conventional manner at 120° C. overnight. The mixture was diluted with water and stirred for 10 min at RT, then neutralized with saturated aq. solution of Na₂CO₃ and extracted with DCM. The organic phase was washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was triturated with EtOAc and dried to afford the product as a white solid (2.02 g, yield 72%). ESI-MS: 265.9 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 10.15 (s, 1H), 8.61 (s, 1H), 8.22-8.15 (m, 1H), 8.06-8.00 (m, 1H), 7.75-7.68 (m, 1H), 3.96 (s, 3H).

Preparation of 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of (3S)-1-(6-methylpyridin-3-yl)piperidin-3-amine (Intermediate 6) (0.44 g, 1.1 eq.), 7-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.56 g, 21.0 eq.), NaOAc (0.173 g, 1.0 eq.) and 4 Å MS in a mixture of anh. MeOH and anh. DCM (1:1; 50.0 mL) was stirred under inert atmosphere at RT overnight. Then, the mixture was cooled to 0° C. and sodium borohydride (0.088 g, 1.1 eq.) was added portionwise. The mixture was allowed to reach RT over 1 h. Subsequently, the mixture was filtered through a pad of Celite, washed with MeOH and the solvent was removed in vacuo. The residue was partitioned between DCM and 10% aq. solution of NaOH, aq. layer was extracted with DCM and the combined organic layers were dried over anh. MgSO₄. Solvents were removed under reduced pressure to provide the product (0.90 g, yield 97%) as a yellow oil which was taken to the next step without additional purification.

Preparation of 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.90 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.32 g, 1.3 eq.) and anh. Na₂SO₄ (3.0 g) in anh. DCE (20.0 mL) was stirred under inert atmosphere at RT overnight. Then, the mixture was cooled to 0° C. and NaBH(OAc)₃ (0.65 g, 1.5 eq.) was added portionwise. The reaction was stirred at RT over 3 h. The mixture was filtered through a pad of Celite pad which was then rinsed with DCM. The filtrate was washed with water, and organic layer was dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH; NH₃) an re-purified by two RP-FCCs (C18HP; H₂O:MeCN) to afford the title compound (0.450 g, yield 40%) as a yellow solid. ESI-MS: 546.3 [M+H]⁺.

Preparation of tert-butyl N-{1-[1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-4-yl}carbamate

Reaction vessel was charged with 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.20 g, 1.0 eq.), tert-butyl N-(piperidin-4-yl)carbamate (0.11 g, 1.5 eq.), Cs₂CO₃ (0.24 g, 2.0 eq.), Pd₂(dba)₃ (0.034 g, 0.1 eq.) and Xantphos (0.042 g, 0.2 eq.). Air was removed and the vessel was backfilled with argon. anh. 1,4-dioxane (5.0 mL) was added and the mixture was stirred at 90° C. overnight. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of Celite and concentrated under reduced pressure. The residue was purified by FCC (SiHP; DCM: MeOH), dissolved in DCM, stirred with MPA scavenger, filtered and evaporated to afford the product (0.16 g, yield 62%) as a yellow oil. ESI-MS: 666.4 [M+H]⁺.

Preparation of 7-(4-aminopiperidin-1-yl)-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A stirred solution of tert-butyl N-{1-[1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-4-yl}carbamate (0.16 g, 1.0 eq.) in 1,4-dioxane (5.0 mL) was treated with 4M HCl in 1,4-dioxane (2.0 mL, 33.3 eq.). The reaction was carried out at 50° C. for 0.5 h, then the mixture was concentrated in vacuo, basified using 2M aq. solution of NaOH and separated by RP-FCC (C18HP; H₂O: MeCN). The isolated product was re-purified by prep-HPLC (H₂O:MeCN; NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.062 mL, 1.0 eq.) and DCM as a solvent (5.0 mL) to provide the title compound (0.071 g, yield 43%) as a yellow solid. ESI-MS: 566.5 [M+H]⁺. ¹H NMR (300 MHz, Deuterium Oxide) δ 8.03-7.97 (m, 1H), 7.93-7.82 (m, 2H), 7.46 (s, 1H), 7.37-7.28 (m, 1H), 7.15-7.02 (m, 2H), 6.93 (d, J=5.4 Hz, 1H), 6.82 (s, 1H), 6.50 (s, 1H), 4.00-3.86 (m, 2H), 3.71-3.52 (m, 5H), 3.50 (s, 3H), 3.49-3.32 (m, 2H), 3.03-2.81 (m, 3H), 2.68-2.43 (m, 2H), 2.35 (s, 3H), 2.16-1.80 (m, 7H), 1.78-1.38 (m, 4H).

Example 7. 7-Amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 7-chloro-7-cyclopropyl-6-fluoro-1,2,3,4-tetrahydroquinolin-4-one

To a cooled solution of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (10.0 g, 1.0 eq.) in anh. MeOH (150.0 mL) NaBH₄ (6.0 g, 4.5 eq.) was added portionwise over 1 h. The mixture was allowed to reach RT and was stirred overnight. Afterwards p-toluenesulfonic acid monohydrate (0.67 g, 0.10 eq.) was added and the reaction mixture was heated at reflux for 3.5 h. Subsequently, the mixture was allowed to reach RT and the solvent was removed in vacuo. The residue was diluted with water and extracted with DCM. Combined organic layers was dried over anh. MgSO₄ and concentrated under reduced pressure. FCC (SiHP; Hex:EtOAc) afforded the product (6.98 g, yield 82%) as a yellow solid. ESI-MS: 240 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 7.55 (d, J=9.3 Hz, 1H), 7.39 (d, J=6.2 Hz, 1H), 3.52 (dd, J=7.5, 6.3 Hz, 2H), 2.61 (dd, J=7.5, 6.3 Hz, 2H), 2.47-2.41 (m, 1H), 0.96-0.86 (m, 2H), 0.76-0.66 (m, 2H).

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

To a mixture of NaOMe (5.67 g, 3.9 eq.) and ethyl formate (8.53 mL, 3.9 eq.) in anh. DCM (140.0 mL) the solution of 7-chloro-1-cyclopropyl-6-fluoro-1,2,3,4-tetrahydroquinolin-4-one (6.45 g, 1.0 eq.) was added and the mixture was stirred under inert atmosphere at RT overnight. Subsequently, the reaction mixture was poured into ice-cold water. The organic layer was washed with 3M aq. solution of NaOH. Combined aq. phases were acidified to pH=6 with concentrated HCl and extracted with DCM. Organic layers were combined, dried over anh. MgSO₄ and concentrated under reduced pressure. The residue was diluted with anh. MeOH (150.0 mL) and MnO₂ (10.6 g, 5.0 eq.) was added. After stirring at RT for 2 days, the mixture was filtered through a pad of Celite and the filter cake was washed with a mixture of DCM and MeOH (1:1). The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM: MeOH). Solvents were evaporated and the residue was redissolved in a small volume of DCM and hexane was added. The precipitate was filtered and dried to provide the product as an off-white solid (3.86 g, yield 54%). ESI-MS: 266.8 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.11 (s, 1H), 8.41 (s, 1H), 8.36 (d, J=6.1 Hz, 1H), 8.09 (d, J=9.1 Hz, 1H), 3.78-3.70 (m, 1H), 1.32-1.24 (m, 2H), 1.22-1.12 (m, 2H).

Preparation of (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2)

To a mixture of (3S)-1-(6-methylpyridin-3-yl)piperidin-3-amine (Intermediate 6)(2.73 g, 1.0 eq.) in anh. DCE (25.0 mL), NaOAc (2.34 g, 2.0 eq.) and 4 Å MS 2-methylpyridine-4-carbaldehyde (1.73, 1.0 eq.) were added. The mixture was stirred at RT overnight. After that the mixture was cooled to 0° C. and sodium borohydride (1.09 g, 2.0 eq.) was added portionwise. The mixture was stirred at RT for 3 h, then concentrated in vacuo. The residue was diluted with DCM and washed with aq. solution of 10% NaOH, brine, dried over anh. MgSO₄ and concentrated in vacuo. The residue was separated by FCC (SiHP; DCM:MeOH). The isolated product was repurified by RP-FCC (C18HP; H₂O:MeCN) to obtain the title compound (3.05 g, yield 34%) as a yellow oil. ESI-MS: 297.1 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.34 (dd, J=5.1, 0.7 Hz, 1H), 8.11 (d, J=2.9 Hz, 1H), 7.25-7.22 (m, 1H), 7.20 (dd, J=8.5, 3.1 Hz, 1H), 7.17 (dd, J=5.1, 1.4 Hz, 1H), 7.04 (d, J=8.5 Hz, 1H), 3.78 (s, 2H), 3.73-3.61 (m, 1H), 3.52-3.44 (m, 1H), 2.71-2.63 (m, 1H), 2.61-2.52 (m, 2H), 2.48-2.42 (m, 4H), 2.36-2.32 (m, 3H), 1.96-1.88 (m, 1H), 1.78-1.68 (m, 1H), 1.57-1.43 (m, 1H), 1.28-1.14 (m, 1H).

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3)

To a solution of (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (2.22 g, 1.0 eq.) in anh. DCE (20.0 mL), 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (1.99 g, 1.0 eq.) was added. The mixture was stirred at RT for 12 h, then cooled to 0° C. NaBH(OAc)₃ (2.22 g, 1.4 eq.) was added portionwise and the reaction was stirred at RT overnight. The mixture was diluted with DCM and washed with H₂O, saturated aq. solution of NaHCO₃, brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to obtain the product (3.20 g, yield 75%) as a yellow solid. ESI-MS: 546.8 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.31-8.24 (m, 1H), 8.16 (d, J=6.2 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 7.97 (d, J=9.5 Hz, 1H), 7.91 (s, 1H), 7.26-7.18 (m, 2H), 7.17-7.13 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 3.82-3.70 (m, 3H), 3.66-3.50 (m, 4H), 2.83-2.69 (m, 2H), 2.65-2.54 (m, 1H), 2.36 (s, 3H), 2.33 (s, 3H), 2.08-1.92 (m, 1H), 1.80-1.72 (m, 1H), 1.62-1.41 (m, 2H), 1.27-1.18 (m, 2H), 1.00-0.82 (m, 2H).

Preparation of 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.05 g, 1.0 eq.), ammonium sulfate (0.024 g, 2.0 eq.) and NaO t-Bu (0.04 g, 4.5 eq.) in anh. 1,4-dioxane (2.0 mL), a solution of Pd[P(o-tol)₃]₂ (0.003 g, 0.05 eq.) and CyPF-t-Bu (0.003 g, 0.05 eq.) in anh. 1,4-dioxane (1.0 mL) was added. The reaction was stirred under inert atmosphere at 100° C. overnight. Next the mixture was diluted with EtOAc, filtered through a pad of Celite and concentrated in vacuo. Separation by FCC (SiHP; DCM:MeOH) and subsequent repurification by RP-FCC (C18HP; H₂O:MeCN) provided the product (0.031 g, yield 63%) as a white solid. ESI-MS: 527.2 [M+H]⁺. ¹H NMR (400 MHz, MeOD-d₄) δ 8.16 (d, J=5.1 Hz, 1H), 8.08 (d, J=2.9 Hz, 1H), 7.79 (s, 1H), 7.75 (d, J=11.9 Hz, 1H), 7.35 (dd, J=8.6, 3.0 Hz, 1H), 7.26-7.19 (m, 3H), 7.11 (d, J=8.6 Hz, 1H), 3.91-3.81 (m, 3H), 3.77 (s, 2H), 3.64-3.57 (m, 1H), 3.40-3.26 (m, overlap with MeOH), 3.02-2.93 (m, 1H), 2.89-2.80 (m, 1H), 2.75-2.63 (m, 1H), 2.41 (s, 3H), 2.34 (s, 3H), 2.17-2.09 (m, 1H), 1.97-1.88 (m, 1H), 1.75-1.58 (m, 2H), 1.28-1.21 (m, 2H), 0.92-0.85 (m, 2H).

Example 8. 7-[(2-Aminoethyl)amino]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 7-[(2-aminoethyl)amino]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), ethylenediamine (0.017 mL, 1.4 eq.) and NaO t-Bu (0.025 g, 1.4 eq.) were dissolved in anh. 1,4-dioxane (3.0 mL). Air was removed, the vessel was backfilled with argon, Pd(OAc)₂ (0.004 g, 0.1 eq.) and BrettPhos (0.02 g, 0.2 eq.) were added and the reaction mixture was stirred under an inert atmosphere at 110° C. for 12 h. Next the mixture was diluted with EtOAc, filtered through Celite pad and concentrated in vacuo. The separation by FCC (SiHP; DCM:MeOH) afforded the product (0.07 g, yield 66%) as a beige solid. ESI-MS: 570.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.0 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 7.71 (s, 1H), 7.61 (d, J=12.3 Hz, 1H), 7.27-7.19 (m, 2H), 7.19-7.16 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 6.92 (d, J=7.5 Hz, 1H), 6.38-6.31 (m, 1H), 3.83-3.70 (m, 3H), 3.63-3.56 (m, 3H), 3.45-3.40 (m, 1H), 3.23-3.18 (m, 2H), 2.83 (t, J=6.3 Hz, 2H), 2.80-2.70 (m, 2H), 2.63-2.54 (m, 1H), 2.39 (s, 3H), 2.33 (s, 3H), 2.01-1.93 (m, 1H), 1.80-1.70 (m, 1H), 1.57-1.42 (m, 2H), 1.24-1.17 (m, 2H), 0.95-0.79 (m, 2H).

Example 9. 1-Cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-Cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a solution of 7-[(2-aminoethyl)amino]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Example 8) (0.048 g, 1.0 eq.) in anh. MeOH (5.0 mL) 37% aq. solution of formaldehyde (0.01 mL) was added. The mixture was stirred at RT for 12 h, then cooled to 0° C. NaBH(OAc)₃ (0.021 g, 1.2 eq.) was added and the reaction was continued overnight at RT. After that the mixture was concentrated under reduced pressure. Then saturated aqueous sodium bicarbonate solution was added and the product was extracted to ethyl acetate. The organic layer was washed with brine, dried over anh. Na₂SO₄ and concentrated under reduced pressure. The residue was purified by FCC (SiHP; DCM:MeOH:NH₃) to afford the product (0.015 g, yield 29%) as a pale yellow powder ESI-MS: 598.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.30 (d, J=5.1 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 7.72 (s, 1H), 7.62 (d, J=12.3 Hz, 1H), 7.23-7.19 (m, 2H), 7.19-7.15 (m, 1H), 7.04-7.00 (m, 1H), 6.94 (d, J=7.4 Hz, 1H), 6.21-6.08 (m, 1H), 3.82-3.71 (m, 3H), 3.62-3.55 (m, 3H), 3.46-3.41 (m, 1H), 3.39-3.26 (m, 2H+HDO), 2.80-2.71 (m, 2H), 2.64-2.57 (m, 1H), 2.57-2.45 (m, 2H+DMSO), 2.39 (s, 3H), 2.33 (s, 3H), 2.22 (s, 6H), 2.02-1.93 (m, 1H), 1.79-1.72 (m, 1H), 1.58-1.44 (m, 2H), 1.25-1.13 (m, 2H), 0.92-0.82 (m, 2H).

Example 10. 7-(2-Aminoethoxy)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-(2-{[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]oxy}ethyl)carbamate

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.15 g, 1.0 eq.), tert-butyl N-(2-hydroxyethyl)carbamate (0.044 g, 1.0 eq.) and Cs₂CO₃ (0.134 g, 1.5 eq.) in anh. toluene (2.5 mL) argon was bubbled for 5 min. After that Pd(OAc)₂ (0.019 g, 0.3 eq.) and t-BuXPhos (0.07 g, 0.6 eq.) were added and the reaction was stirred under an inert atmosphere at 50° C. over weekend. Subsequently the mixture was filtered through Celite pad and concentrated in vacuo. The residue was partitioned between water and DCM. Organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and solvents were evaporated. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.097 g, yield 53%) as a pale yellow solid. ESI-MS: 671.8 [M+H]⁺.

Preparation of 7-(2-aminoethoxy)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a solution of tert-butyl N-(2-{[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]oxy}ethyl)carbamate (0.097 g, 1.0 eq.) in 1,4-dioxane (10.0 mL) was added 4M HCl in 1,4-dioxane (2.02 mL, 56.0 eq.) and the mixture was stirred at 50° C. for 75 min. Subsequently the reaction was poured into water and basified with 2N NaOH and extracted with DCM. Organic layer was dried over anh. Na₂SO₄, filtered and evaporated. The residue was purified by FCC (SiHP; DCM:MeOH). The compound was converted into HCl salt using 2M HCl in Et₂O (0.058 mL, 1.0 eq.) and a mixture of MeOH (5.0 mL) and H₂O (1.0 mL) as a solvent to provide the product (0.065 g, yield 76%) as a yellow solid. ESI-MS: 571.2 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.20-8.11 (m, 3H), 7.89-7.81 (m, 2H), 7.52 (d, J=7.2 Hz, 1H), 7.27-7.20 (m, 2H), 7.18-7.15 (m, 1H), 7.04 (d, J=8.5 Hz, 1H), 4.45 (t, J=5.0 Hz, 2H), 3.84-3.70 (m, 3H), 3.65-3.57 (m, 3H), 3.56-3.48 (m, 1H), 3.39-3.28 (m, 2H+HDO), 2.81-2.72 (m, 2H), 2.65-2.55 (m, 1H), 2.37 (s, 3H), 2.33 (s, 3H), 2.01-1.94 (m, 1H), 1.82-1.74 (m, 1H), 1.58-1.45 (m, 2H), 1.29-1.22 (m, 2H), 0.94-0.85 (m, 2H).

Example 11. 1-Cyclopropyl-6-fluoro-7-[2-(methylamino)ethoxy]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-(2-{[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]oxy}ethyl)-N-methylcarbamate

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.12 g, 1.0 eq.), tert-butyl N-(2-hydroxyethyl)-N-methylcarbamate (0.039 g, 1.0 eq.) and Cs₂CO₃ (0.107 g, 1.5 eq.) in anh. toluene (2.0 mL) argon was bubbled for 5 min. After that tBuXPhos (0.056 g, 0.6 eq.) and Pd(OAc)₂ (0.015 g, 0.3 eq.) were added and the reaction mixture was stirred at 50° C. overnight. Subsequently the mixture was filtered through a pad of Celite. The filtrate was concentrated in vacuo and partitioned between water and DCM. The organic layer was washed with brine and dried over anh. Na₂SO₄, filtered and evaporated. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to provide the product (0.042 g, yield 28%) as a pale brown solid. ESI-MS: 685.9 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.28 (d, J=5.1 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.85 (s, 1H), 7.79 (d, J=11.6 Hz, 1H), 7.62-7.41 (m, 1H), 7.30-7.07 (m, 3H), 7.02 (d, J=8.5 Hz, 1H), 4.45-4.25 (m, 2H), 3.91-3.71 (m, 3H), 3.71-3.47 (m, 6H), 2.99-2.83 (m, 3H), 2.78-2.68 (m, 2H), 2.66-2.54 (m, 2H), 2.37 (s, 3H), 2.32 (s, 3H), 2.04-1.87 (m, 1H), 1.85-1.68 (m, 1H), 1.62-1.43 (m, 2H), 1.43-1.18 (m, 10H), 1.02-0.76 (m, 2H).

Preparation of 1-cyclopropyl-6-fluoro-7-[2-(methylamino)ethoxy]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Tert-butyl N-(2-{[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]oxy}ethyl)-N-methylcarbamate (0.042 g, 1.0 eq.) was dissolved in DCM (4.0 mL) and 2M HCl in Et₂O was added dropwise (1.07 mL, 35.0 eq.) and the mixture was stirred at RT for 1 h. Subsequently the reaction was poured into water and basified with 2N NaOH. The product was extracted to DCM. Organic layer was dried over Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH:NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.027 mL, 1.0 eq.) and a mixture of MeOH (5.0 mL) and H₂O (1.0 mL) as a solvent to provide the product (0.031 g, 0.050 mmol, yield 84%) as a yellow solid. ESI-MS: 585.2 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (br m, 3H), 8.14 (d, J=2.9 Hz, 1H), 7.88-7.82 (m, 2H), 7.52 (d, J=7.2 Hz, 1H), 7.25-7.20 (m, 2H), 7.17 (d, J=5.3 Hz, 1H), 7.03 (d, J=8.5 Hz, 1H), 4.48 (t, J=5.0 Hz, 2H), 3.83-3.74 (m, 3H), 3.65-3.57 (m, 3H), 3.57-3.49 (m, 1H), 3.38 (t, J=5.0 Hz, 2H), 2.81-2.72 (m, 2H), 2.63 (s, 3H), 2.61-2.55 (m, 1H), 2.38 (s, 3H), 2.33 (s, 3H), 2.02-1.94 (m, 1H), 1.81-1.73 (m, 1H), 1.58-1.45 (m, 2H), 1.30-1.20 (m, 2H), 0.96-0.84 (m, 2H).

Example 12. 7-[(3R)-3-Aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]carbamate

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), tert-butyl N-[(3R)-pyrrolidin-3-yl]carbamate (0.051 g, 1.5 eq.) and Cs₂CO₃ (0.119 g, 2.0 eq.) in anh. 1,4-dioxane (2.7 mL) argon was bubbled for 5 min. After that Pd₂(dba)₃*CHCl₃ (0.038 g, 0.2 eq.) and Xantphos (0.032 g, 0.3 eq.) were added and the reaction mixture was stirred at 115° C. overnight. The reaction mixture was filtered through a pad of Celite, evaporated and purified by FCC (SiHP deactivated with NH₃:DCM; DCM:MeOH) to provide the product (0.13 g) as a yellow solid which was used in the next step without further purification. ESI-MS: 696.7 [M+H]⁺.

Preparation of 7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

To a solution of tert-butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]carbamate (0.13 g, 1.0 eq.) in 1,4-dioxane (3.5 mL) was added 4M HCl in 1,4-dioxane (3.0 mL) and the mixture was stirred at 55° C. for 30 min. Then the solvents were evaporated and the residue was partitioned between DCM and aq. solution of NaHCO₃. The aqueous layer was additionally extracted with DCM. The combined organic layers were dried over anh. MgSO₄, filtered and evaporated. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.09 mL, 2.0 eq.) and DCM as a solvent (5.0 mL) to provide the product (0.06 g, yield 48%) as a pale yellow solid. ESI-MS: 596.3 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.50-8.41 (m, 1H), 8.20-8.06 (m, 2H), 7.97 (dd, J=9.1, 3.0 Hz, 1H), 7.92-7.83 (m, 2H), 7.70-7.54 (m, 2H), 7.00 (d, J=7.5 Hz, 1H), 4.90-4.68 (m, 2H+H₂O), 4.65-4.44 (m, 2H), 4.23-4.11 (m, 1H), 4.06-3.80 (m, 5H), 3.79-3.70 (m, 1H), 3.69-3.60 (m, 1H), 3.60-3.46 (m, 2H), 3.33-3.21 (m, 1H), 2.57 (s, 3H), 2.55 (s, 3H), 2.54-2.45 (m, 1H), 2.44-2.32 (m, 1H), 2.32-2.19 (m, 2H), 2.18-2.06 (m, 1H), 1.96-1.79 (m, 1H), 1.43-1.25 (m, 2H), 1.12-0.88 (m, 2H).

Example 13. 7-[(3S)-3-Aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]carbamate

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), tert-butyl N-[(3S)-pyrrolidin-3-yl]carbamate (0.051 g, 1.5 eq.) and Cs₂CO₃ (0.119 g, 2.0 eq.) in anh. 1,4-dioxane (2.7 mL) argon was bubbled for 5 min. After that Pd₂(dba)₃ (0.034 g, 0.2 eq.) and Xantphos (0.032 g, 0.3 eq.) were added and the reaction mixture was stirred at 115° C. for 3 days. The reaction mixture was filtered through a pad of Celite, evaporated and purified by FCC (SiHP deactivated with NH₃:DCM; DCM:MeOH) to provide the product (0.10 g, yield 78%) as a yellow solid which was used in the next step without further purification. ESI-MS: 696.7 [M+H]⁺.

Preparation of 7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

To a solution of tert-butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]carbamate (0.10 g, 1.0 eq.) in 1,4-dioxane (2.7 mL) was added 4M HCl in 1,4-dioxane (3.0 mL) and the mixture was stirred at 55° C. for 1 h. Then the solvents were evaporated and the residue was partitioned between DCM and aq. solution of NaOH. The combined organic layers were dried over anh. MgSO₄, filtered and evaporated. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.04 mL, 2.0 eq.) and DCM as a solvent (5.0 mL) to provide the product (0.024 g, yield 25%) as a pale yellow solid. ESI-MS: 596.3 [M+H]⁺. ¹H NMR (400 MHz, D₂O) δ 8.39-8.33 (m, 1H), 8.05-8.00 (m, 2H), 7.87 (dd, J=9.1, 2.9 Hz, 1H), 7.81-7.77 (m, 2H), 7.54 (d, J=14.2 Hz, 1H), 7.50 (d, J=9.1 Hz, 1H), 6.91 (d, J=7.5 Hz, 1H), 4.79-4.61 (m, 2H+H₂O), 4.55-4.34 (m, 2H), 4.12-4.04 (m, 1H), 4.01-3.92 (m, 1H), 3.92-3.73 (m, 4H), 3.66-3.52 (m, 2H), 3.50-3.36 (m, 2H), 3.24-3.15 (m, 1H), 2.48 (s, 3H), 2.47 (s, 3H), 2.45-2.37 (m, 1H), 2.29-2.12 (m, 3H), 2.10-1.96 (m, 1H), 1.88-1.73 (m, 1H), 1.29-1.21 (m, 2H), 0.98-0.83 (m, 2H).

Example 14. 1-Cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methyl pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-Cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.054 g, 1.0 eq.), (3R)-pyrrolidin-3-ol (0.026 g, 3.0 eq.), and Cs₂CO₃ (0.068 g, 2.1 eq.) in anh. DMF (3.0 mL) argon was bubbled for 5 min. After that Pd₂(dba)₃*CHCl₃ (0.020 g, 0.2 eq.) and BINAP (0.018 g, 0.3 eq.) were added and the reaction mixture was stirred at 115° C. overnight. Subsequently the reaction mixture was combined with a mixture from a parallel reaction performed following the same protocol and starting from 0.030 g of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) and using corresponding equivalents of remaining reagents. The combined mixtures were filtered through a pad of Celite the pad was washed with DCM. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH), re-dissolved in DCM and stirred with MPA scavenger for 10 min. Subsequently the mixture was filtered, concentrated in vacuo and purified by prep-HPLC (H₂O:MeCN:NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.044 mL, 1.05 eq.) and a mixture of MeOH (5.0 mL) and H₂O (1.0 mL) as a solvent to provide the product (0.053 g, yield 56%) as a yellow solid. ESI-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, Pyridine-d₅) δ 8.63-8.59 (m, 1H), 8.57 (d, J=3.0 Hz, 1H), 8.42 (d, J=14.6 Hz, 1H), 7.94 (s, 1H), 7.42-7.36 (m, 2H), 7.33 (dd, J=8.5, 3.0 Hz, 1H), 7.06 (d, J=8.5 Hz, 1H), 6.83 (d, J=7.7 Hz, 1H), 4.75-4.70 (m, 1H), 4.20-4.12 (m, 1H), 4.08 (s, 2H), 3.94-3.75 (m, 5H), 3.57-3.46 (m, 2H), 3.28-3.20 (m, 1H), 3.19-3.11 (m, 1H), 2.93-2.83 (m, 1H), 2.59-2.47 (m, 7H), 2.23-2.03 (m, 3H), 1.73-1.64 (m, 1H), 1.60-1.48 (m, 2H), 1.13-1.03 (m, 2H), 0.97-0.89 (m, 2H).

Example 15. 7-[(3R)-3-(Aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-{[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]methyl}carbamate

Through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), tert-butyl N-{[(3S)-pyrrolidin-3-yl]methyl}carbamate (0.055 g, 1.5 eq.) and Cs₂CO₃ (0.119 g, 2.0 eq.) in anh. 1,4-dioxane (2.7 mL) argon was bubbled for 5 minutes. After that Pd₂(dba)₃ (0.034 g, 0.2 eq.) and Xantphos (0.032 g, 0.3 eq.) were added and the reaction mixture was stirred at 115° C. overnight. The mixture was filtered through a pad of Celite and purified by FCC (SiHP deactivated with NH₃:DCM; DCM:MeOH), triturated with Et₂O and dried to provide the product (0.08 g, yield 61%) as a pale yellow solid. ESI-MS: 710.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.1 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.72 (s, 1H), 7.62 (d, J=14.6 Hz, 1H), 7.25-7.17 (m, 2H), 7.20-7.13 (m, 1H), 7.08-6.98 (m, 2H), 6.84 (d, J=7.7 Hz, 1H), 3.84-3.69 (m, 3H), 3.62-3.45 (m, 6H), 3.45-3.35 (m, 1H), 3.29-3.20 (m, 1H), 3.08-2.98 (m, 2H), 2.79-2.68 (m, 2H), 2.63-2.53 (m, 1H), 2.38 (s, 4H), 2.32 (s, 3H), 2.10-1.90 (m, 2H), 1.81-1.64 (m, 2H), 1.58-1.43 (m, 2H), 1.38 (s, 9H), 1.21-1.15 (m, 2H), 0.92-0.78 (m, 2H).

Preparation of 7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

To a solution of tert-butyl N-{[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]methyl}carbamate (0.057 g, 1.0 eq.) in 1,4-dioxane (1.5 mL) was added 4M HCl in 1,4-dioxane (1.0 mL) and the mixture was stirred at 55° C. for 1 h. Then 7N NH₃ in MeOH was added and the solvents were evaporated. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃). The compound was converted to HCl salt using 2M HCl in Et₂O (0.07 mL, 2.0 eq.) and DCM as a solvent (5.0 mL) to provide the product (0.045 g, yield 81%) as a pale yellow solid. ESI-MS: 610.3 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.41-8.37 (m, 1H), 8.02-7.98 (m, 2H), 7.85 (dd, J=9.1, 2.9 Hz, 1H), 7.83-7.79 (m, 2H), 7.48 (dd, J=11.6, 2.6 Hz, 2H), 6.85-6.77 (m, 1H), 4.83-4.62 (m, 43H), 4.56-4.35 (m, 2H), 3.91-3.77 (m, 3H), 3.74-3.65 (m, 1H), 3.65-3.56 (m, 2H), 3.48-3.33 (m, 3H), 3.31-3.19 (m, 1H), 3.17-3.03 (m, 2H), 2.71-2.57 (m, 1H), 2.49 (s, 3H), 2.47 (s, 3H), 2.30-2.18 (m, 3H), 2.09-1.97 (m, 1H), 1.88-1.73 (m, 2H), 1.28-1.20 (m, 2H), 0.98-0.83 (m, 2H).

Example 16. 7-[(3S)-3-Aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-3-yl]carbamate

A reaction vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.050 g, 1.0 eq.), tert-butyl N-[(3S)-piperidin-3-yl]carbamate (0.037 g, 2.0 eq.), Cs₂CO₃ (0.063 g, 2.1 eq.), Pd₂(dba)₃*CHCl₃ (0.019 g, 0.2 eq.) and BINAP (0.017 g, 0.3 eq.). Next the vessel was capped, air was removed and the vessel was filled with argon. Then anh. DMF (2.0 mL) was added and the reaction mixture was stirred at 115° C. overnight. The mixture was filtered through a pad of Celite and purified by FCC (SiHP; DCM:MeOH) and repurified by prep-HPLC (H₂O:MeCN:NH₃) to afford the product (0.041 g, yield 59%) as a white solid. ESI-MS: 710.6 [M+H]⁺.

Preparation of 7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a solution of tert-butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-3-yl]carbamate (0.041 g, 1.0 eq.) in 1,4-dioxane (3.0 mL) was added 4M HCl in 1,4-dioxane (2.0 mL) and the mixture was stirred at 50° C. for 1 h. Then the solvents were evaporated. The residue was suspended in water, basified with 2M aq. solution of NaOH, evaporated, purified by prep-HPLC (H₂O:MeCN:NH₃) and re-purified by FCC (C18HP; H₂O:MeCN) to provide the product (0.010 g, yield 28%) as a light yellow solid. ESI-MS: 610.3 [M+H]⁺. ¹H NMR (400 MHz, MeOD-d₄) δ 8.17 (d, J=5.1 Hz, 1H), 8.10 (d, J=3.0 Hz, 1H), 7.93 (s, 1H), 7.84 (d, J=13.4 Hz, 1H), 7.43 (d, J=7.4 Hz, 1H), 7.36 (dd, J=8.6, 3.0 Hz, 1H), 7.27-7.20 (m, 2H), 7.13 (d, J=8.6 Hz, 1H), 3.92-3.84 (m, 3H), 3.82-3.78 (m, 2H), 3.65-3.55 (m, 2H), 3.51-3.42 (m, 2H), 3.21-3.11 (m, 1H), 3.08-2.93 (m, 2H), 2.93-2.81 (m, 2H), 2.75-2.66 (m, 1H), 2.42 (s, 3H), 2.35 (s, 3H), 2.20-2.10 (m, 1H), 2.08-2.00 (m, 1H), 2.00-1.87 (m, 2H), 1.87-1.75 (m, 1H), 1.75-1.59 (m, 2H), 1.56-1.43 (m, 1H), 1.35-1.25 (m, 2H), 0.99-0.88 (m, 2H).

Example 17. 7-[(3R)-3-Aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-3-yl]carbamate

A reaction vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), tert-butyl N-[(3R)-piperidin-3-yl]carbamate (0.073 g, 2.0 eq.), Cs₂CO₃ (0.125 g, 2.1 eq.), Pd₂(dba)₃*CHCl₃ (0.038 g, 0.2 eq.) and BINAP (0.034 g, 0.3 eq.). Next the vessel was capped, air was removed and the vessel was filled with argon. Then anh. DMF (3.0 mL) was added and the reaction mixture was stirred at 115° C. overnight. The mixture was filtered through a pad of Celite and the pad was washed with DCM. The filtrate was evaporated and the residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to afford the product (0.052 g, yield 38%) as a white powder. ESI-MS: 710.6 [M+H]⁺.

Preparation of 7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a solution of tert-butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-3-yl]carbamate (0.052 g, 1.0 eq.) in 1,4-dioxane (3.0 mL) was added 4M HCl in 1,4-dioxane (2.0 mL) and the mixture was stirred at 50° C. for 1 h. Then the solvents were evaporated. The residue was suspended in water, basified with 2M aq. solution of NaOH, evaporated, purified by prep-HPLC (H₂O:MeCN:NH₃) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to provide the product (0.01 g, yield 22%) as a white solid. ESI-MS: 610.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.17 (d, J=5.1 Hz, 1H), 8.10 (d, J=3.0 Hz, 1H), 7.93 (s, 1H), 7.85 (d, J=13.4 Hz, 1H), 7.44 (d, J=7.4 Hz, 1H), 7.37 (dd, J=8.6, 3.0 Hz, 1H), 7.26-7.21 (m, 2H), 7.13 (d, J=8.6 Hz, 1H), 3.93-3.82 (m, 3H), 3.81 (s, 2H), 3.65-3.54 (m, 2H), 3.52-3.38 (m, 2H), 3.30-3.22 (m, 1H), 3.13-3.05 (m, 1H), 3.03-2.94 (m, 2H), 2.86 (t, J=11.1 Hz, 1H), 2.75-2.67 (m, 1H), 2.42 (s, 3H), 2.34 (s, 3H), 2.19-2.10 (m, 1H), 2.10-1.92 (m, 3H), 1.91-1.76 (m, 1H), 1.75-1.53 (m, 3H), 1.35-1.27 (m, 2H), 0.98-0.88 (m, 2H).

Example 18. 1-(2-Aminoethyl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-[2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)ethyl]-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

To a mixture of 4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1) (0.050 g, 1.0 eq.), K₂CO₃ (0.112 g, 2.8 eq.) and DMF (3.0 mL) under nitrogen atmosphere were added 2-(2-bromoethyl)-2,3-dihydro-1H-isoindole-1,3-dione (0.205 g, 2.8 eq.) and KI (0.134 g, 2.8 eq.). The reaction mixture was heated at 90° C. for 18 h. Then the reaction was quenched with addition of H₂O and aq. solution of sodium bicarbonate and the mixture with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.050 g, yield 17%) as a white powder. ESI-MS: 347.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.07 (s, 1H), 8.54 (s, 1H), 8.31 (dd, J=8.0, 1.6 Hz, 1H), 7.96 (d, J=8.5 Hz, 1H), 7.86-7.81 (m, 5H), 7.54 (ddd, J=8.0, 7.0, 0.9 Hz, 1H), 4.74-4.67 (m, 2H), 4.03-3.98 (m, 2H).

Preparation of 2-{2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]ethyl}-2,3-dihydro-1H-isoindole-1,3-dione

A dry reactor vessel was charged with 1-[2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)ethyl]-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.045 g, 1.0 eq.), (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.039 g, 1.0 eq.) and anh. DCE (6.0 mL). The reaction was carried out at 80° C. for 2 h, then cooled to 0° C. and then NaBH(OAc)₃ (0.039 g, 1.4 eq.) was added. The reaction was continued for 48 h at RT. Additional portion of NaBH(OAc)₃ (0.028 g, 1.0 eq.) was added and the reaction was stirred for 6 h at RT, and then at 50° C. for 12 h. Subsequently the reaction was quenched with addition of H₂O and aq. solution of sodium bicarbonate and the mixture was extracted with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.035 g, yield 42%) as a yellow solid. ESI-MS: 627.7 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.25 (d, J=5.0 Hz, 1H), 8.20 (dd, J=8.1, 1.6 Hz, 1H), 8.09 (d, J=2.9 Hz, 1H), 7.86 (s, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.75-7.67 (m, 5H), 7.38-7.33 (m, 1H), 7.15 (dd, J=8.6, 3.0 Hz, 1H), 7.11 (s, 1H), 7.10-7.07 (m, 1H), 7.00 (d, J=8.5 Hz, 1H), 4.66-4.51 (m, 2H), 4.00-3.92 (m, 2H), 3.76-3.65 (m, 1H), 3.59-3.43 (m, 5H), 2.63-2.53 (m, 2H), 2.42 (s, 3H), 2.32 (s, 3H), 1.73-1.57 (m, 2H), 1.38-1.25 (m, 3H).

Preparation of 1-(2-aminoethyl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

To a solution of 2-{2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]ethyl}-2,3-dihydro-1H-isoindole-1,3-dione (0.03 g, 1.0 eq.) in absolute EtOH (5.0 mL) was added hydrazine monohydrate (0.005 g, 2.0 eq.) and the mixture was stirred at reflux for 12 h under argon atmosphere. The mixture was concentrated in vacuo and the residue was purified by RP-FCC (C18HP; H₂O:MeCN) to afford the product (0.018 g, yield 74%) as a yellow powder. ESI-MS: 497.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.0 Hz, 1H), 8.20 (dd, J=8.1, 1.6 Hz, 1H), 8.14 (d, J=3.0 Hz, 1H), 8.05 (s, 1H), 7.74 (d, J=8.6 Hz, 1H), 7.68 (ddd, J=8.6, 6.8, 1.7 Hz, 1H), 7.34 (ddd, J=7.9, 6.8, 1.0 Hz, 1H), 7.27-7.19 (m, 3H), 7.01 (d, J=8.5 Hz, 1H), 4.29-4.18 (m, 2H), 3.90-3.72 (m, 3H), 3.66-3.56 (m, 3H), 2.91-2.83 (m, 2H), 2.81-2.71 (m, 2H), 2.62-2.53 (m, 2H), 2.39 (s, 3H), 2.32 (s, 3H), 2.04-1.97 (m, 1H), 1.81-1.40 (m, 4H).

Example 19. 1-cyclopropyl-6-fluoro-7-[4-(methylamino)piperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-{1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-4-yl}-N-methylcarbamate

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.12 g, 1.0 eq.), tert-butyl N-methyl-N-(piperidin-4-yl)carbamate (0.094 g, 2.0 eq.), Cs₂CO₃ (0.15 g, 2.1 eq.) and DMF (2.0 mL). The resulting mixture was purged with argon for 5 min. Then, BINAP (0.041 g, 0.3 eq.) and Pd₂(dba)₃*CHCl₃ (0.023 g, 0.1 eq.) were added and the reaction mixture was stirred overnight at 120° C. The mixture was then filtered through a pad of Celite and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) to give the product (0.14 g, 83% yield) as a yellow oil. ESI-MS: 724.8 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[4-(methylamino)piperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Tert-butyl N-{1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-4-yl}-N-methylcarbamate (0.14 g, 1.0 eq.) was dissolved in DCM (5.0 mL) and TFA (1.0 mL) was added. The reaction mixture was stirred overnight at RT. Then, the mixture was concentrated in vacuo. To the residue, water was added followed by saturated aq. solution of NaHCO₃ and the resulting mixture was washed with DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃) and re-purified by prep-HPLC (H₂O:MeCN:TFA). The obtained sample was dissolved in DCM, washed with saturated aq. solution of NaHCO₃, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.3 mL, 1.0 eq. to FB) and DCM as a solvent (5.0 mL) to give the product (0.038 g, 29% yield) as a yellow solid. ESI-MS: 624.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=4.9 Hz, 1H), 8.12 (d, J=2.9 Hz, 1H), 7.82 (s, 1H), 7.71 (d, J=13.4 Hz, 1H), 7.34 (d, J=7.5 Hz, 1H), 7.23-7.19 (m, 2H), 7.18-7.15 (m, 1H), 7.02 (d, J=8.6 Hz, 1H), 3.82-3.69 (m, 3H), 3.66-3.55 (m, 5H), 3.51-3.45 (m, 1H), 3.07-2.96 (m, 1H), 2.91-2.81 (m, 2H), 2.79-2.69 (m, 2H), 2.62-2.55 (m, 1H), 2.52 (s, 3H), 2.37 (s, 3H), 2.32 (s, 3H), 2.12-2.07 (m, 2H), 1.99-1.92 (m, 1H), 1.79-1.73 (m, 1H), 1.70-1.60 (m, 2H), 1.55-1.44 (m, 2H), 1.24-1.16 (m, 2H), 0.95-0.83 (m, 2H).

Example 20. 7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-{[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]methyl}carbamate

7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.13 g, 1.0 eq.), tert-butyl N-{[(3R)-pyrrolidin-3-yl]methyl}carbamate hydrochloride (0.085 g, 1.5 eq.) and Cs₂CO₃ (0.248 g, 3.2 eq.) were suspended in anh. 1,4-dioxane (3.5 mL) and the mixture was purged with argon. Under inert atmosphere, Pd₂(dba)₃ (0.044 g, 0.2 eq.) and Xantphos (0.041 g, 0.3 eq.) were added and the reaction mixture was stirred overnight at 115° C. Subsequently, the mixture was cooled to ambient temperature and filtered through a pad of celite. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; MeCN:H₂O) to afford (0.1 g, 59% yield) as a white solid. ESI-MS: 710.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.72 (s, 1H), 7.62 (d, J=14.6 Hz, 1H), 7.25-7.13 (m, 3H), 7.07-6.97 (m, 2H), 6.84 (d, J=7.8 Hz, 1H), 3.83-3.67 (m, 3H), 3.63-3.44 (m, 6H), 3.43-3.35 (m, 1H), 3.28-3.21 (m, 1H), 3.11-2.96 (m, 2H), 2.81-2.68 (m, 2H), 2.63-2.53 (m, 1H), 2.44-2.35 (m, 4H), 2.32 (s, 3H), 2.06-1.91 (m, 2H), 1.80-1.65 (m, 2H), 1.58-1.42 (m, 2H), 1.38 (s, 9H), 1.21-1.13 (m, 2H), 0.92-0.76 (m, 2H).

Preparation of 7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

4M HCl in 1,4-dioxane solution (1.5 mL) was added to a solution of tert-butyl N-{[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]methyl}carbamate (0.078 g, 1.0 eq.) in 1,4-dioxane (2.0 mL). The mixture was stirred at 55° C. for 1 h and subsequently concentrated in vacuo. The residue was partitioned between DCM and aq. solution of NaOH. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP deactivated with NH₃; DCM:MeOH). The obtained sample was converted to HCl salt using 2M HCl in Et₂O solution and DCM as solvent but due to insufficient purity it was re-purified by prep-HPLC (H₂O:MeCN:NH₃) and converted once again to the HCl salt using 2 M HCl in Et₂O solution (0.02 mL) and DCM (5.0 mL) as a solvent to provide the product (0.013 g, 17% yield) as a pale yellow solid. ESI-MS: 610.3 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.14-7.98 (m, 2H), 7.81 (s, 1H), 7.75-7.63 (m, 1H), 7.56 (d, J=14.4 Hz, 1H), 7.43-7.32 (m, 1H), 7.31-7.24 (m, 1H), 7.26-7.15 (m, 1H), 6.86-6.77 (m, 1H), 4.29-3.88 (m, 4H), 3.88-3.76 (m, 2H), 3.72-3.56 (m, 2H), 3.56-3.46 (m, 1H), 3.45-3.27 (m, 3H), 3.26-3.09 (m, 3H), 3.08-2.93 (m, 1H), 2.79-2.61 (m, 1H), 2.49 (s, 3H), 2.38-2.14 (m, 5H), 2.10-1.91 (m, 2H), 1.90-1.80 (m, 1H), 1.80-1.70 (m, 1H), 1.33-1.21 (m, 2H), 0.91-0.78 (m, 2H).

Example 21. 3-({[(3S)-7-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(2-methoxyethyl)-7,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]carbamate

A mixture of 5-bromo-2-nitropyridine (3.6 g, 1.0 eq.), tert-butyl N-[(3S)-piperidin-3-yl]carbamate (4.62 g, 1.3 eq.), Cs₂CO₃ (7.8 g, 1.35 eq.) and 1,4-dioxane (96.0 mL) was purged with argon for 5 min. Then, Xantphos (0.616 g, 0.06 eq.) and Pd₂(dba)₃ (0.812 g, 0.05 eq.) were added and the reaction mixture was stirred at 115° C. for 3 days. Afterwards, the reaction mixture was cooled to ambient temperature, filtered through a pad of celite and concentrated in vacuo. The residue was dissolved in DCM and stirred overnight with scavenger QuadraPure MPA (3.0 g). The mixture was filtered and concentrated in vacuo. The residue was purified by two subsequent FCCs (SiHP; Hex:EtOAc) to give the product (3.7 g, 65% yield) as a yellow solid. ESI-MS: 323.4 [M+H]⁺.

Preparation of (3S)-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 12)

4M HCl solution in 1,4-dioxane (23.0 mL, 8.0 eq.) to a solution of tert-butyl N-[(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]carbamate (3.7 g, 1.0 eq.) in 1,4-dioxane (25.0 mL) was added. The resulting slurry was stirred at 55° C. for 1 h. Then, the reaction mixture was concentrated in vacuo and the residue was partitioned between DCM and 10% NaOH aq. solution. The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the product (2.5 g, 62% yield) as a yellow solid which was used directly in the next step. ESI-MS: 223.2 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)

A solution of 2-methylpyridine-4-carbaldehyde (0.24 mL, 1.0 eq.), (3S)-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 12)(0.50 g, 1.5 eq.) and NaOAc (0.176 g, 1.0 eq.) in anh. MeOH (7.0 mL) was stirred overnight at RT under an inert atmosphere. Then, the solution was cooled to 0° C. and NaBH₄ (0.09 g, 1.1 eq.) was added in in portions over 5 minutes. The resulting mixture was left stirring for 1 h at RT. Afterwards, the reaction mixture was filtered through a pad of celite, the pad was washed with MeOH and the filtrate was concentrated in vacuo. The residue was partitioned between DCM and aq. solution of NaOH. The organic layer was washed with brine, dried over anh. Na₂SO₄ and filtered. Solvents were removed under reduced pressure and the residue was purified by FCC (SiHP deactivated with NH₃; DCM:MeOH) to give the product (0.65 g, 78% yield) as a yellow solid. ESI-MS: 328.5 [M+H]⁺.

Preparation of 1-(2-methoxyethyl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

A suspension of 4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1) (0.7 g, 1.0 eq.)

and K₂CO₃ (1.56 g, 2.8 eq.) in anh. DMF (20.0 mL) was stirred for 10 min at RT under inert atmosphere. Then, KI (1.88 g, 2.8 eq.) and 1-bromo-2-methoxyethane (1.1 mL, 2.8 eq.) were added and the resulting mixture was stirred for 18 h at 90° C. Afterwards, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.7 g, 75% yield) as a beige solid. ESI-MS: 232.6 [M+H]⁺. ¹H NMR (300 MHz, Chloroform-d) δ 10.45 (s, 1H), 8.63-8.53 (m, 1H), 8.38 (s, 1H), 7.82-7.71 (m, 1H), 7.58-7.48 (m, 2H), 4.42 (t, J=5.1 Hz, 2H), 3.80 (t, J=5.1 Hz, 2H), 3.34 (s, 3H).

Preparation of 1-(2-methoxyethyl)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)(0.13 g, 1.0 eq.) and 1-(2-methoxyethyl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.10 g, 1.1 eq.) in DCE (3.0 mL) was stirred for 1.5 h at 55° C. Then, NaBH(OAc)₃ (0.21 g, 2.5 eq.) was added in portions and the resulting mixture was stirred for another 3.5 h at 55° C. Afterwards, the mixture was diluted with DCM and NaOH aq. solution and the product was extracted to DCM. Organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.083 g, 39% yield) as a yellow solid. ESI-MS: 543.8 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(2-methoxyethyl)-1,4-dihydroquinolin-4-one Hydrochloride

A solution of 1-(2-methoxyethyl)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.08 g, 1.0 eq.) in MeOH (3.0 mL) was purged with argon and Pd/C (10 wt. %, 0.008 g) was added. The mixture was stirred for 2 h under hydrogen atmosphere at RT. Then, the reaction mixture was filtered through a pad of celite, concentrated in vacuo and the residue was purified by RP-FCC (C18HP; H₂O:MeCN). The obtained sample was converted into HCl salt using 2 M HCl solution in Et₂O (0.059 mL, 1.0 eq. to FB) and DCM (3.0 mL) as a solvent to give the product (0.045 g, 56% yield) as a pale yellow solid. ESI-MS: 513.4 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.01 (dd, J=8.2, 1.5 Hz, 1H), 7.90 (d, J=5.5 Hz, 1H), 7.81 (s, 1H), 7.67 (ddd, J=8.6, 7.0, 1.6 Hz, 1H), 7.54 (d, J=8.8 Hz, 1H), 7.46 (dd, J=9.2, 2.9 Hz, 1H), 7.43-7.40 (m, 1H), 7.40-7.34 (m, 1H), 7.13-7.02 (m, 2H), 6.66 (d, J=9.2 Hz, 1H), 4.30 (t, J=5.1 Hz, 2H), 3.94-3.70 (m, 4H), 3.66 (t, J=5.0 Hz, 2H), 3.50-3.40 (m, 1H), 3.14 (s, 5H), 2.81-2.66 (m, 1H), 2.64-2.51 (m, 1H), 2.08 (s, 3H), 2.05-1.95 (m, 1H), 1.94-1.82 (m, 1H), 1.70-1.49 (m, 2H).

Example 22. 7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-{5-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-5-azaspiro[2.4]heptan-7-yl}carbamate

Argon was bubbled for 5 min through a suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.114 g, 1.0 eq.), tert-butyl N-{5-azaspiro[2.4]heptan-7-yl}carbamate (0.088 g, 2.0 eq.) and Cs₂CO₃ (0.143 g, 2.1.0 eq.) in DMF (3.0 mL). Then BINAP (0.039 g, 0.3 eq.) and Pd₂(dba)₃*CHCl₃ (0.043 g, 0.2 eq.). The resulting mixture was stirred overnight at 115° C. Afterwards the mixture was filtered through a pad of celite and then rinsed with DCM. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP, DCM:MeOH). The isolated sample was dissolved in DCM and stirred with for 20 min with scavenger QuadraPure MPA. The scavenger was filtered off and the filtrate was concentrated to give the product (0.105 g, 70% yield) as a yellow oil. AP-MS: 722.7 [M+H]⁺.

Preparation of 7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

4 M HCl in 1,4-dioxane (1.1 mL) was added to a solution of tert-butyl N-{5-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-5-azaspiro[2.4]heptan-7-yl}carbamate (0.105 g, 1.0 eq.) in 1,4-dioxane (6.0 mL) and the mixture was stirred for 1 h at RT. Then, the reaction mixture was poured into water. The pH of the resulting mixture was adjusted to ˜11 with 2 M aq. solution of NaOH and washed with DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated under reduced pressure. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.062 mL, 2.0 eq. to FB) and DCM as a solvent (2.4 mL) to provide the desired product (0.039 g, 41% yield) as a white solid. ESI-MS: 622.3 [M+H]⁺. ¹H NMR (400 MHz, Deuterium Oxide) δ 8.05-7.95 (m, 1H), 7.93-7.83 (m, 1H), 7.69-7.58 (m, 1H), 7.58-7.47 (m, 1H), 7.41-7.29 (m, 1H), 7.14-7.03 (m, 1H), 6.98-6.89 (m, 1H), 6.89-6.80 (m, 1H), 6.77-6.67 (m, 1H), 4.10-3.95 (m, 1H), 3.95-3.80 (m, 2H), 3.79-3.53 (m, 4H), 3.50-3.45 (m, 1H), 3.44-3.33 (m, 1H), 3.23-3.10 (m, 2H), 3.06-2.84 (m, 1H), 2.84-2.52 (m, 2H), 2.31 (s, 3H), 2.07-1.92 (m, 4H), 1.91-1.80 (m, 1H), 1.70-1.40 (m, 2H), 1.25-1.05 (m, 3H), 1.05-0.93 (m, 1H), 0.95-0.83 (m, 2H), 0.83-0.72 (m, 1H), 0.72-0.59 (m, 2H).

Example 23. 7-[(3R)-3-hydroxypyrrolidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 13)

7-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 8) (0.5 g, 1.0 eq.) and (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.56 g, 1.0 eq.) were suspended in anh. DCE (15.0 mL) and the reaction mixture was stirred overnight at RT. Then, NaBH(OAc)₃ (0.56 g, 1.4 eq.) was added and the stirring was continued for an additional 24 h. Afterwards, the reaction mixture was diluted with DCM, washed with sat. aq. sodium bicarbonate, water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) to give the product (0.495 g, 46% yield) as a yellow solid. ESI-MS: 546.3, 548.2 [M+H]⁺.

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A pressure vessel was charged with 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 73) (0.08 g, 1.0 eq.), (3R)-pyrrolidin-3-ol (0.035 mL, 3.0 eq.), Cs₂CO₃ (0.1 g, 2.1 eq.) in DMF (3.0 mL). The mixture was purged with argon for 5 min and then, BINAP (0.027 g, 0.3 eq.) and Pd₂(dba)₃ (0.03 g, 0.2 eq.) were added and the reaction mixture was stirred at 115° C. for 6 h. Subsequently, the mixture was filtered through a pad of celite, washed with DCM and the filtrate was concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN) to afford the product (0.025 g, 22% yield) as a yellow solid. ESI-MS: 553.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ8.29 (d, J=5.0 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 7.95 (d, J=8.9 Hz, 1H), 7.79 (s, 1H), 7.29-7.17 (m, 3H), 7.01 (d, J=8.5 Hz, 1H), 6.73-6.61 (m, 1H), 6.27-6.13 (m, 1H), 5.03 (d, J=3.7 Hz, 1H), 4.49-4.37 (m, 1H), 3.89-3.65 (m, 6H), 3.62-3.36 (m, 6H), 3.26-3.18 (m, 1H), 2.81-2.64 (m, 2H), 2.63-2.54 (m, 1H), 2.40 (s, 3H), 2.32 (s, 3H), 2.13-2.01 (m, 1H), 2.00-1.89 (m, 2H), 1.80-1.69 (m, 1H), 1.56-1.37 (m, 2H).

Example 24. 3-({[(3S)-7-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-7-(propan-2-yl)-7,4-dihydroquinolin-4-one Hydrochloride

Preparation of 2,4,5-trifluorobenzoyl Chloride

2,4,5-Trifluorobenzoic acid (0.5 g, 1.0 eq.) was suspended in SOCl₂ (1.15 mL, 5.6 eq.) and the resulting mixture was stirred for 1.5 h at 80° C. Afterwards, the reaction mixture was concentrated with DCM in vacuo and the isolated product (0.55 g, 98.5% yield) was used directly in the next step without further purification.

Preparation of ethyl 3-(dimethylamino)-2-(2,4,5-trifluorobenzoyl)prop-2-enoate

A solution of 2,4,5-trifluorobenzoyl chloride (0.55 g, 1.0 eq.) in toluene (5.0 mL) was added to a stirring at RT mixture of ethyl 3-(dimethylamino)prop-2-enoate (0.41 mL, 1.0 eq.) and DIPEA (1.0 mL, 2.1 eq.) over 5 min. The resulting mixture was stirred for 3 h at 90° C. Then, the mixture was partitioned between DCM and water. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by FCC (SiHP; EtOAc) to give the product (0.21 g, 24% yield) as a yellow oil. ESI-MS: 302.1 [M+H]⁺.

Preparation of ethyl 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylate

A mixture of ethyl 3-(dimethylamino)-2-(2,4,5-trifluorobenzoyl)prop-2-enoate (0.65 g, 1.0 eq.), isopropylamine (0.22 mL, 1.3 eq.) and toluene (5.0 mL) was heated for 1.5 h at 110° C. Afterwards, the reaction mixture was concentrated in vacuo and DMF (5.0 mL) was added to the residue followed by K₂CO₃ (0.74 g, 2.5 eq.). The resulting mixture was heated overnight at 100° C. Then, the mixture was cooled to RT and partitioned between DCM and water. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.311 g, 47% yield) as a yellow solid. ESI-MS: 296.4 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.60 (s, 1H), 8.21 (dd, J=13.1, 6.6 Hz, 1H), 8.11 (dd, J=10.8, 9.1 Hz, 1H), 5.01 (hept, J=6.4 Hz, 1H), 4.24 (q, J=7.1 Hz, 2H), 1.49 (d, J=6.5 Hz, 6H), 1.28 (t, J=7.1 Hz, 3H).

Preparation of 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylic Acid

Ethyl 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylate (0.29 g, 1.0 eq.) was suspended in a 1 M HCl aq. solution (6.0 mL, 6.1 eq.). The resulting mixture was stirred at 50° C. for 1 h and additionally for 3 h at 95° C. ESI-MS: 268.3 [M+H]⁺. Subsequently, the reaction mixture was concentrated under reduced pressure to give the product (0.24 g, 87% yield) as a yellow solid. ¹H NMR (300 MHz, DMSO-d₆) δ 14.89 (br, 1H), 8.89 (s, 1H), 8.47 (dd, J=13.0, 6.7 Hz, 1H), 8.30 (dd, J=10.5, 8.8 Hz, 1H), 5.19 (hept, J=6.6 Hz, 1H), 1.56 (d, J=6.5 Hz, 6H).

Preparation of 6,7-difluoro-1-(propan-2-yl)-1,2,3,4-tetrahydroquinolin-4-one

6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylic acid (0.24 g, 1.0 eq.) was dissolved in MeOH (4.0 mL) and the mixture was cooled in an ice-bath. Then, NaBH₄ (0.15 g, 4.5 eq.) was added in portions and the reaction mixture was left stirring overnight at RT. Subsequently, PTSA monohydrate (0.017 g, 0.1 eq.) was added and stirring was continued for 3 h. Then, the reaction mixture was concentrated in vacuo and the residue was partitioned between DCM and water. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.20 g, 96% yield) as a bright yellow solid. ESI-MS: 226.2 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 7.58 (dd, J=11.0, 9.6 Hz, 1H), 7.13 (dd, J=14.6, 6.6 Hz, 1H), 4.16 (h, J=6.6 Hz, 1H), 3.43-3.35 (m, 2H), 2.62-2.50 (m, 2H), 1.16 (d, J=6.6 Hz, 6H).

Preparation of 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinoline-3-carbaldehyde

A solution of 6,7-difluoro-1-(propan-2-yl)-1,2,3,4-tetrahydroquinolin-4-one (0.1 g, 1.0 eq.) in anh. DCM (5.0 mL) was added to a mixture of MeONa (0.094 g, 3.9 eq.) and ethyl formate (0.14 mL, 3.94 eq.) at RT and the resulting mixture was stirred overnight. Subsequently, the reaction mixture was washed with 2 M NaOH aq. solution. The aqueous layer was acidified using 1 M HCl (aq. Solution) and washed with DCM. The organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the product (0.1 g, 64% yield) as a yellow solid that was used directly in the next step without further purification. ESI-MS: 254.1 [M+H]⁺.

Preparation of 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde

6,7-Difluoro-4-oxo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinoline-3-carbaldehyde (0.1 g, 1.0 eq.) was dissolved in anh. MeOH (5.0 mL) and MnO₂ (0.17 g, 5.0 eq.) was added. The resulting mixture was stirred overnight at RT. Then, the reaction mixture was filtered through a pad of celite and the pad was washed carefully with MeOH. The filtrate was concentrated in vacuo to give the product (0.077 g, 74% yield) as a yellow solid. ESI-MS: 252.3 [M+H]⁺.

Preparation of 6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

A dry pressure vessel was charged with 6,7-difluoro-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (0.077 g, 1.0 eq.), (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)(0.1 g, 1.0 eq.) and anh. DCE (3.0 mL). The resulting mixture was stirred for 1 h at 70° C. Then, the mixture was cooled to RT and NaBH(OAc)₃ (0.182 g, 2.8 eq.) was added in portions. Heating was continued at 65° C. for 1 h and then, the mixture was left stirring over the weekend at RT. Afterwards, the reaction mixture was partitioned between DCM and NaHCO₃ aq. solution. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.1 g, 35% yield) as a yellow solid. ESI-MS: 563.8 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1-(propan-2-yl)-1,4-dihydroquinolin-4-one Hydrochloride

A mixture of 6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.1 g, 1.0 eq.), iron powder (0.03 g, 3 eq.), NH₄Cl (0.05 g, 5.0 eq.) in EtOH (6.0 mL) and H₂O (0.5 mL) was stirred at reflux for 1 h. Then, the reaction mixture was cooled to RT, diluted with MeOH, filtered through a pad of celite and concentrated in vacuo. The residue was purified by RP-FCC (C18HP, H₂O:MeCN). The compound obtained was converted into its HCl salt using 2 M HCl in Et₂O (0.014 mL, 1.0 eq. to FB) and DCM (2.0 mL) to provide the product (0.015 g, 14% yield) as a yellow solid. ESI-MS: 533.3 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.18-8.07 (m, 3H), 7.88 (dd, J=12.9, 6.6 Hz, 1H), 7.74 (dd, J=9.5, 2.8 Hz, 1H), 7.37 (d, J=2.8 Hz, 1H), 7.27 (s, 1H), 7.25-7.20 (m, 1H), 6.82 (d, J=9.4 Hz, 1H), 4.98-4.90 (m, 1H), 3.89-3.73 (m, 4H), 3.70-3.63 (m, 1H), 3.40-3.33 (m, 1H), 3.01-2.91 (m, 1H), 2.78-2.69 (m, 1H), 2.61-2.52 (m, 1H), 2.37 (s, 3H), 2.14-2.06 (m, 1H), 1.95-1.88 (m, 1H), 1.70-1.56 (m, 2H), 1.46 (d, J=6.5 Hz, 6H).

Example 25. 2-amino-N-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]acetamide

Preparation of 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 14)

Pd₂(dba)₃ (0.042 g, 0.1 eq.) and Me₄tButylXphos (0.022 g, 0.1 eq.) were added to a mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.25 g, 1.0 eq.) and NaO-t-Bu (0.062 g, 1.4 eq.) in ammonia solution 0.5 M in 1,4-dioxane (13.7 mL). The reaction mixture was stirred at 100° C. for 16 h. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of celite and washed with an aq. solution of NaOH. The organic layer was dried over anh. Na₂SO₄ and solvent was evaporated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.137 g, 56% yield) as a yellow powder. ESI-MS: 527.8 [M+H]⁺.

Preparation of 2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetic Acid

The reaction vessel was charged with glycine (0.30 g, 1.0 eq.), phthalic anhydride (0.89 g, 1.5 eq.), triethylamine (0.607 g, 1.5 eq.), toluene (5.0 mL) and 4 Å MS. The mixture was heated to reflux, stirred for 10 h and then concentrated in vacuo. The resulting white solid was taken up in water (50.0 mL) and the mixture was acidified with conc. HCl (3.0 mL). The product was collected by filtration, washed with water (2×30.0 mL) and freeze-dried to provide the product (0.65 g, 28% yield) as a white powder. ESI-MS: 203.9 [M−H]⁻. ¹H NMR (400 MHz, DMSO-d₆) δ 13.28 (s, 1H), 7.97-7.87 (m, 4H), 4.32 (s, 2H).

Preparation 2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetyl Chloride

In an oven dried reactor, 2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetic acid (0.25 g, 1.0 eq.) and anh. DCE (6.0 mL) were introduced. Thionyl chloride (0.13 mL, 1.5 eq.) was then added, and the mixture refluxed at 80° C. for 12 h. Subsequently, the mixture was concentrated in vacuo and product was used immediately after this workup as the reactant for the next step.

Preparation of N-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetamide

Triethylamine (0.032 mL, 2.5 eq.) and 2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetyl chloride (0.26 g, 10.0 eq.) were sequentially added to a mixture of 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 14) (0.06 g, 1.0 eq.) in CHCl₃ (3.0 mL). and the reaction mixture was stirred at RT for 12 h. Afterwards, the reaction was quenched with water and the mixture washed with DCM. The organic layer was dried over anh. Na₂SO₄ and solvent was removed in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to provide the product (0.037 g, 34% yield) as a beige powder. ESI-MS: 714.6 [M+H]⁺.

Preparation 2-amino-N-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]acetamide

Hydrazine monohydrate (0.004 g, 2.0 eq.) was added to a solution of N-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-2-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)acetamide (0.025 g, 1.0 eq.) in EtOH (5.0 mL). The mixture was stirred at reflux for 12 h and subsequently concentrated in vacuo. The residue was purified by FCC (C18HP; H₂O:MeCN) to provide the product (0.009 g, 41% yield) as a yellow powder. ESI-MS: 584.4 [M+H]⁺. 1H NMR (400 MHz, DMSO-d6) δ 9.07 (d, J=6.7 Hz, 1H), 8.28 (d, J=5.1 Hz, 1H), 8.15-8.12 (m, 1H), 7.89-7.84 (m, 2H), 7.25-7.20 (m, 2H), 7.18-7.15 (m, 1H), 7.03 (d, J=8.5 Hz, 1H), 5.44-4.96 (m, 2H), 3.84-3.74 (m, 3H), 3.65-3.62 (m, 2H), 3.62-3.57 (m, 1H), 3.51-3.44 (m, 1H), 3.39-3.39 (m, 2H), 2.79-2.73 (m, 2H), 2.64-2.57 (m, 1H), 2.37 (s, 3H), 2.33 (s, 3H), 2.02-1.95 (m, 1H), 1.81-1.74 (m, 1H), 1.59-1.45 (m, 2H), 1.26-1.15 (m, 2H), 0.95-0.84 (m, 2H).

Example 26. 7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.29 g, 1.0 eq.), tert-butyl N-(3,3-difluoropiperidin-4-yl)carbamate (0.25 g, 2.0 eq.), Cs₂CO₃ (0.36 g, 2.1 eq.) and DMF (3.0 mL). The mixture was purged with argon for 5 min. Then, BINAP (0.099 g, 0.3 eq.) and Pd₂(dba)₃ (0.055 g, 0.1 eq.) were added and the resulting mixture was stirred overnight at 115° C. Afterwards, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by two consecutive RP-FCCs (C18HP, H₂O:MeCN) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.026 g, 7% yield) as a white solid. ESI-MS: 646.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.82 (s, 1H), 7.72 (d, J=13.4 Hz, 1H), 7.37 (d, J=7.6 Hz, 1H), 7.25-7.18 (m, 2H), 7.18-7.12 (m, 1H), 7.01 (d, J=8.5 Hz, 1H), 3.87-3.67 (m, 4H), 3.65-3.46 (m, 5H), 3.39-3.26 (m, 1H, overlapping with water peak), 3.20-3.05 (m, 2H), 2.82-2.63 (m, 2H), 2.63-2.54 (m, 1H), 2.37 (s, 3H), 2.32 (s, 3H), 2.02-1.89 (m, 2H), 1.85-1.60 (m, 4H), 1.59-1.39 (m, 2H), 1.30-1.15 (m, 2H), 0.97-0.81 (m, 2H).

Example 27. 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.15 g, 1.0 eq.), (azetidin-3-yl)methanol hydrochloride (0.068 g, 2.0 eq.), Cs₂CO₃ (0.188 g, 2.1 eq.) and DMF (5.0 mL) and the mixture was purged with argon for 5 min. Then, BINAP (0.051 g, 0.3 eq.) and Pd₂(dba)₃ (0.028 g, 0.1 eq.) were added and the resulting mixture was stirred overnight at 115° C. Afterwards, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was dissolved in DCM, the scavenger QuadraPure MPA was added and the mixture was stirred for 10 min at RT. Subsequently, it was filtered, concentrated in vacuo and the residue purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.07 g, 42% yield) as a yellow solid. ESI-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.1 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 7.73 (s, 1H), 7.62 (d, J=13.2 Hz, 1H), 7.24-7.15 (m, 3H), 7.02 (d, J=8.5 Hz, 1H), 6.68 (d, J=7.9 Hz, 1H), 4.83 (t, J=5.3 Hz, 1H), 4.14-4.08 (m, 2H), 3.87-3.82 (m, 2H), 3.81-3.69 (m, 3H), 3.64-3.56 (m, 5H), 3.44-3.38 (m, 1H), 2.89-2.81 (m, 1H), 2.79-2.70 (m, 2H), 2.62-2.55 (m, 1H), 2.39 (s, 3H), 2.33 (s, 3H), 1.99-1.93 (m, 1H), 1.79-1.72 (m, 1H), 1.56-1.43 (m, 2H), 1.21-1.16 (m, 2H), 0.90-0.80 (m, 2H).

Example 28. 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.15 g, 1.0 eq.), (3R)-piperidin-3-ol hydrochloride (0.056 g, 2.0 eq.), Cs₂CO₃ (0.188 g, 2.1 eq.) and DMF (5.0 mL) and the mixture was purged with argon. Then, BINAP (0.051 g, 0.3 eq.) and Pd₂(dba)₃ (0.028 g, 0.1 eq.) were added and the reaction mixture was stirred overnight at 115° C. Afterwards, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was dissolved in DCM, the scavenger QuadraPure MPA added and the resulting mixture was stirred for 10 min at RT. Subsequently the mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃). The isolated sample was converted to HCl salt using 2 M HCl in Et₂O solution (0.033 mL, 1.0 eq. to FB) and DCM (2.0 mL) as a solvent to give the product (0.037 g, 20% yield) as a yellow solid. ESI-MS: 611.4 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.20 (d, J=5.4 Hz, 1H), 8.15 (d, J=3.0 Hz, 1H), 7.89 (s, 1H), 7.81 (d, J=13.5 Hz, 1H), 7.78-7.71 (m, 1H), 7.50-7.29 (m, 4H), 4.09-3.77 (m, 6H), 3.76-3.60 (m, 2H), 3.53-3.40 (m, 2H), 3.11-2.90 (m, 3H), 2.90-2.74 (m, 2H), 2.52 (s, 3H), 2.37 (s, 3H), 2.21-2.11 (m, 1H), 2.10-2.02 (m, 1H), 2.00-1.89 (m, 2H), 1.81-1.60 (m, 3H), 1.54-1.43 (m, 1H), 1.35-1.24 (m, 2H), 1.01-0.88 (m, 2H).

Example 29. 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 7-bromo-1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

NaBH(OAc)₃ (0.29 g, 1.5 eq.) was added in portions to a mixture of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)(0.3 g, 1.0 eq.) and 7-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 8) (0.245 g, 1.0 eq.) in DCE (6.0 mL). The reaction mixture was stirred overnight at RT. Then, an additional portion of NaBH(OAc)₃ (0.20 g, 1.0 eq.) was added and stirring was continued for 3 h. The reaction mixture was diluted with DCM, washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) to afford the product (0.28 g, 47% yield) as a yellow solid. ESI-MS: 577.3, 578.6 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one Hydrochloride

A mixture of 7-bromo-1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.28 g, 1.0 eq.), iron powder (0.081 g, 3.0 eq.), NH₄Cl (0.13 g, 5.0 eq.), EtOH (6.0 mL) and H₂O (0.5 mL) was stirred at reflux for 1 h. The reaction mixture was cooled to RT, diluted with DCM and filtered through a pad of celite. The pad was washed with DCM and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified twice by RP-FCC (C18HP, MeCN:H₂O). The obtained compound was converted to the HCl salt using 2 M HCl in Et₂O (0.094 mL, 1.0 eq. to FB) with DCM as the solvent (5.0 mL) to give the product (0.108 g, 38% yield) as a yellow solid. ESI-MS: 547.1, 549.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.1 Hz, 1H), 8.08 (d, J=8.6 Hz, 1H), 7.96 (s, 1H), 7.85 (d, J=1.7 Hz, 1H), 7.56-7.47 (m, 3H), 7.25-7.17 (m, 2H), 6.65-6.58 (m, 1H), 6.40 (brs, 2H) 3.82 (s, 3H), 3.76-3.71 (m, 2H), 3.62-3.53 (m, 3H), 3.29-3.26 (m, 1H), 2.80-2.70 (m, 1H), 2.65-2.59 (m, 1H), 2.47-2.40 (m, 1H), 2.36 (s, 3H), 2.02-1.93 (m, 1H), 1.80-1.71 (m, 1H), 1.54-1.37 (m, 2H).

Example 30. 7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-({1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-3-fluoropyrrolidin-3-yl}methyl)carbamate

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.24 g, 1.0 eq.), tert-butyl N-[(3-fluoropyrrolidin-3-yl)methyl]carbamate (0.144 g, 1.5 eq.), Cs₂CO₃ (0.46 g, 3.2 eq.) in anh. 1,4-dioxane (6.4 mL) was purged with argon for 5 min. Then, Pd₂(dba)₃ (0.08 g, 0.2 eq.) and Xantphos (0.076 g, 0.3 eq.) were added under inert atmosphere. The resulting mixture was purged with argon for additional 5 min and then heated overnight at 115° C. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH). The obtained sample was dissolved in DCM, the scavenger QuadraPure MPA (0.35 g) was added and the mixture was left stirring overnight at RT. Then, the mixture was filtered and concentrated in vacuo to give the product (0.21 g, 66% yield) as a yellow solid. ESI-MS: 728.4 [M+H]⁺.

Preparation of 7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

4 M HCl in 1,4-dioxane solution (2.2 mL, 30.0 eq.) was added to a solution of tert-butyl N-({1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-3-fluoropyrrolidin-3-yl}methyl)carbamate (0.209 g, 1.0 eq.) in 1,4-dioxane (5.6 mL). The resulting mixture was stirred at 55° C. for 1 h and subsequently concentrated in vacuo. The residue was partitioned between DCM and NaOH aq. solution. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.13 g, 72% yield) as a white solid. ESI-MS: 628.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.12 (d, J=2.9 Hz, 1H), 7.74 (s, 1H), 7.65 (d, J=14.6 Hz, 1H), 7.25-7.18 (m, 2H), 7.18-7.14 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 6.90 (d, J=7.8 Hz, 1H), 3.89-3.53 (m, 10H), 3.48-3.38 (m, 1H), 3.03-2.85 (m, 2H), 2.81-2.68 (m, 2H), 2.62-2.52 (m, 1H), 2.38 (s, 3H), 2.32 (s, 3H), 2.25-2.10 (m, 2H), 2.05-1.92 (m, 1H), 1.94-1.59 (m, 3H), 1.60-1.38 (m, 2H), 1.29-1.15 (m, 2H), 0.92-0.75 (m, 2H).

Example 31. 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.15 g, 1.0 eq.), (3S)-piperidin-3-ol hydrochloride (0.113 g, 3.0 eq.), Cs₂CO₃ (0.2 g, 2.1 eq.) and anh. DMF (4.0 mL) was purged with argon for 5 min. Then, Pd₂(dba)₃ (0.05 g, 0.2 eq.) and BINAP (0.051 g, 0.3 eq.) were added. The resulting mixture was additionally purged with argon and left stirring overnight at 115° C. Subsequently, it was cooled to ambient temperature, filtered through a pad of celite and the filtrate concentrated in vacuo. The residue was dissolved in DCM and scavenger QuadraPure MPA was added. The resulting mixture was stirred overnight at RT, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep-HPLC (H₂O:MeCN:NH₃). The obtained sample was converted into HCl salt using 2 M HCl in Et₂O (0.024 mL, 1.0 eq. to FB) and DCM (5.0 mL) as a solvent to give the product (0.029 g, 16% yield) as a pale yellow solid. ESI-MS: 611.4 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.20 (d, J=5.4 Hz, 1H), 8.15 (d, J=2.9 Hz, 1H), 7.88 (s, 1H), 7.81 (d, J=13.5 Hz, 1H), 7.74-7.69 (m, 1H), 7.43-7.34 (m, 3H), 7.34-7.30 (m, 1H), 4.07-3.76 (m, 6H), 3.74-3.62 (m, 2H), 3.53-3.41 (m, 2H), 3.10-2.89 (m, 3H), 2.88-2.76 (m, 2H), 2.51 (s, 3H), 2.36 (s, 3H), 2.22-2.13 (m, 1H), 2.11-2.01 (m, 1H), 2.00-1.90 (m, 2H), 1.82-1.63 (m, 3H), 1.56-1.43 (m, 1H), 1.33-1.25 (m, 2H), 0.97-0.90 (m, 2H).

Example 32. 1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.15 g, 1.0 eq.), 4-hydroxypiperidine (0.083 g, 3.0 eq.), Cs₂CO₃ (0.18 g, 2.0 eq.) and BINAP (0.051 g, 0.3 eq.) was dried on a rotary evaporator under reduced pressure for 15 min. Then anh. DMF (3.0 mL) was added and the resulting mixture was stirred for 16 h at 115° C. under inert atmosphere. Afterwards, the reaction mixture was diluted with EtOAc and filtered through a pad of celite. The filtrate was concentrated in vacuo and partitioned between water and EtOAc. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH). The isolated sample was dissolved in DCM and stirred for 15 min with scavenger QuadraPure MPA. The scavenger was filtered off and the filtrate was concentrated in vacuo and the residue was additionally purified by prep-HPLC (H₂O:MeCN:NH₃). The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.032 mL, 1.0 eq. to FB) and DCM as a solvent (10.0 mL) to provide the product (0.040 g, 93% yield) as a yellow powder. ESI-MS: 611.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.23-8.20 (m, 1H), 8.19-8.15 (m, 1H), 7.92-7.88 (m, 1H), 7.86-7.80 (m, 1H), 7.78-7.73 (m, 1H), 7.45-7.33 (m, 4H), 4.08-3.78 (m, 6H), 3.76-3.68 (m, 1H), 3.66-3.57 (m, 2H), 3.51-3.43 (m, 1H), 3.12-2.99 (m, 4H), 2.91-2.81 (m, 1H), 2.57-2.50 (m, 3H), 2.42-2.35 (m, 3H), 2.23-2.15 (m, 1H), 2.10-1.93 (m, 3H), 1.82-1.64 (m, 4H), 1.36-1.27 (m, 2H), 0.99-0.92 (m, 2H).

Example 33. 1-cyclopropyl-6-fluoro-7-[3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-{1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl}-N-methylcarbamate

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.22 g, 1.0 eq.), tert-butyl N-methyl-N-(pyrrolidin-3-yl)carbamate (0.12 g, 1.5 eq.), Cs₂CO₃ (0.42 g, 3.2 eq.) and anh. 1,4-dioxane (5.9 mL) was purged with argon for 5 min. Then, Pd₂(dba)₃ (0.074 g, 0.2 eq.) and Xantphos (0.07 g, 0.3 eq.) were added. The resulting mixture was again purged with argon for a few minutes and left stirring overnight at 115° C. Subsequently, the mixture was cooled to ambient temperature, filtered through a pad of celite, the pad was washed with DCM and the filtrate was concentrated in vacuo. The residue was dissolved in DCM, scavenger QuadraPure MPA was added and the mixture was left stirring overnight at RT. Then, the mixture was filtered and concentrated in vacuo. The residue was purified by FCC (SiHP deactivated with NH₃; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.21 g, 73% yield) as a white solid. ESI-MS: 710.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

4 M HCl solution in 1,4-dioxane (3.0 mL, 44.0 eq.) was added to a solution of tert-butyl N-{1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl}-N-methylcarbamate (0.194 g, 1.0 eq.) in 1,4-dioxane (5.2 mL) and the resulting mixture was stirred for 1 h at 55° C. Then, the reaction mixture was concentrated in vacuo and the residue was basified to pH ˜11 with 7 M NH₃ solution in MeOH and purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.09 g, 54% yield) as a yellowish solid. ESI-MS: 610.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.15 (d, J=5.2 Hz, 1H), 8.08 (d, J=2.9 Hz, 1H), 7.81 (s, 1H), 7.75 (d, J=14.8 Hz, 1H), 7.34 (dd, J=8.6, 3.0 Hz, 1H), 7.24 (s, 1H), 7.23-7.17 (m, 1H), 7.10 (d, J=8.6 Hz, 1H), 6.91 (d, J=7.6 Hz, 1H), 3.89-3.68 (m, 7H), 3.64-3.54 (m, 2H), 3.47-3.34 (m, 3H), 3.00-2.89 (m, 1H), 2.89-2.78 (m, 1H), 2.71-2.62 (m, 1H), 2.44 (s, 3H), 2.40 (s, 3H), 2.35 (s, 3H), 2.30-2.20 (m, 1H), 2.18-2.09 (m, 1H), 1.98-1.85 (m, 2H), 1.74-1.57 (m, 2H), 1.30-1.20 (m, 2H), 0.96-0.81 (m, 2H).

Example 34. 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridinyl-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 3-(dimethylamino)-1-(2-nitrophenyl)prop-2-en-1-one

A solution of 1-(2-nitrophenyl)ethan-1-one (0.81 mL, 1.0 eq.) and (dimethoxymethyl)dimethylamine (0.77 mL, 0.95 eq.) in DMF (5.0 mL) was heated for 3 h at 100° C. Then, the reaction mixture was concentrated under reduced pressure, and the solid formed was washed with Et₂O and collected by filtration to give the product (1.14 g, 80% yield) as a yellow solid. ESI-MS: 221.3 [M+H]⁺.

Preparation of 1-(propan-2-yl)-1,4-dihydroquinolin-4-one

A mixture of 3-(dimethylamino)-1-(2-nitrophenyl)prop-2-en-1-one (0.8 g, 1.0 eq.), K₂CO₃ (1.4 g, 3.0 eq.) in DMF (6.0 mL) was heated for 2 h at 100° C., and then heating was continued for 3 days at the same temperature. Afterwards, the reaction mixture was cooled to RT and partitioned between EtOAc and water. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.43 g, 65% yield) as a yellow solid. ESI-MS: 188.1 [M+H]⁺.

Preparation of 4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde

A mixture of 1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.43 g, 1.0 eq.), HMT (0.64 g, 2.0 eq.) and TFA (6.0 mL) was heated overnight at 100° C. Then, the reaction mixture was cooled to RT and ice-cold water was added. The resulting mixture was stirred for 10 min, then basified with sat. Na₂CO₃ aq. solution and washed with DCM. The organic layers was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) give the product (0.28 g, 57% yield) as a white solid. ESI-MS: 216.3 [M+H]⁺.

Preparation of 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

A pressure vessel charged with (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)(0.15 g, 1.0 eq.), 4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (0.099 g, 1.0 eq.) and DCE (3.0 mL) was treated with NaBH(OAc)₃ (0.27 g, 2.8 eq.) in portions. The resulting mixture was stirred overnight at RT and then partitioned between water and DCM. The organic layer was washed with NaHCO₃, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.18 g, 61% yield) as a yellow solid. ESI-MS: 527.7 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one Hydrochloride

A mixture of 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.18 g, 1.0 eq.), iron powder (0.06 g, 3.0 eq.), NH₄Cl (0.09 g, 5.0 eq.), EtOH (6.0 mL) and H₂O (0.5 mL) was stirred at reflux for 1 h. Then, the reaction mixture was cooled to RT, diluted with MeOH, filtered through a pad of celite and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The obtained sample was converted into the HCl salt using 2 M HCl in Et₂O (0.102 mL, 1.0 eq. to FB) and DCM (2.0 mL) as a solvent to give the product (0.094 g, 51% yield) as a brownish solid. ESI-MS: 497.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.37 (dd, J=8.2, 1.6 Hz, 1H), 8.24-8.15 (m, 1H), 8.11 (s, 1H), 7.91-7.82 (m, 2H), 7.76 (ddd, J=8.7, 6.9, 1.7 Hz, 1H), 7.45 (ddd, J=8.0, 6.9, 0.9 Hz, 1H), 7.40-7.26 (m, 3H), 6.90 (d, J=9.5 Hz, 1H), 5.09 (hept, J=6.6 Hz, 1H), 4.06-3.78 (m, 4H), 3.78-3.67 (m, 1H), 3.43-3.35 (m, 1H), 3.11-2.93 (m, 1H), 2.86-2.72 (m, 1H), 2.70-2.52 (m, 1H), 2.36 (s, 3H), 2.18-2.06 (m, 1H), 2.01-1.87 (m, 1H), 1.77-1.56 (m, 2H), 1.50 (d, J=6.6 Hz, 6H).

Example 35. 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(oxetan-3-yl)-1,4-dihydroquinolin-4-one

Preparation of 1-(oxetan-3-yl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1) (0.5 g, 1.0 eq.) was added to a mixture of 3-iodooxetane (0.73 g, 1.4 eq.) and K₂CO₃ (1.6 g, 4.0 eq.) in DMF (13.3 mL) was added. The resulting mixture was stirred for 24 h at 120° C., then an additional portion of 3-iodooxetane (0.5 g, 0.94 eq.) was added and heating was continued for an additional 27 h at 120° C. Afterwards, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.139 g, 21% yield) as a beige solid. ESI-MS: 230.1 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.20 (s, 1H), 8.47 (s, 1H), 8.34 (dd, J=8.0, 1.6 Hz, 1H), 7.83 (ddd, J=8.7, 7.1, 1.7 Hz, 1H), 7.60-7.55 (m, 1H), 7.51 (d, J=8.5 Hz, 1H), 5.82 (quint, J=6.9 Hz, 1H), 5.13-5.06 (m, 2H), 5.00-4.94 (m, 2H).

Preparation of 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(oxetan-3-yl)-1,4-dihydroquinolin-4-one

A mixture of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11)(0.095 g, 1.0 eq.), 1-(oxetan-3-yl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.073 g, 1.1 eq.) and anh. DCE (3.0 mL) was stirred for 1.5 h at 60° C. Then, NaBH(OAc)₃ (0.154 g, 2.5 eq.) was added in portions and the resulting mixture was stirred for 3 h at 60° C. Subsequently, the reaction mixture was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to obtain the product (0.12 g, 76% yield) as a yellow solid. ESI-MS: 541.6 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(oxetan-3-yl)-1,4-dihydroquinolin-4-one

A solution of 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1-(oxetan-3-yl)-1,4-dihydroquinolin-4-one (0.12 g, 1.0 eq.) in MeOH (3.0 mL) was degassed in vacuo and purged with argon (3 times). Then, Pd/C (10 wt. %, 0.012 g) was added in one portion and the resulting mixture was purged again with argon (twice). Then, hydrogen was introduced and the mixture was left stirring for 2 h under the hydrogen atmosphere at RT. Then, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.037 g, 33% yield) as a white solid. ESI-MS: 511.4 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.40-8.35 (m, 1H), 8.22 (s, 1H), 8.21-8.18 (m, 1H), 7.74 (ddd, J=8.7, 7.0, 1.6 Hz, 1H), 7.67-7.64 (m, 1H), 7.46 (ddd, J=8.1, 7.0, 0.9 Hz, 1H), 7.40 (d, J=8.7 Hz, 1H), 7.35 (dd, J=8.9, 2.9 Hz, 1H), 7.32-7.24 (m, 2H), 6.58 (dd, J=8.9, 0.7 Hz, 1H), 5.82-5.73 (m, 1H), 5.25-5.17 (m, 2H), 4.88-4.83 (m, 2H), 3.92-3.86 (m, 4H), 3.68-3.58 (m, 1H), 3.32-3.26 (m, 1H), 3.09-2.96 (m, 1H), 2.76 (t, J=10.8 Hz, 1H), 2.63-2.53 (m, 1H), 2.35 (s, 3H), 2.22-2.11 (m, 1H), 1.98-1.88 (m, 1H), 1.78-1.54 (m, 2H).

Example 36. 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-1,2,3,4-tetrahydro-1,8-naphthyridin-4-one

NaBH₄ (6.0 g, 4.5 eq.) was added slowly to a solution of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydro-1,8-naphthyridine-3-carboxylic acid (10.0 g, 1.0 eq.) in MeOH (150.0 mL) kept under inert atmosphere in an ice cooling bath. The mixture was allowed to warm to RT and was left stirring for 16 h. Then, PTSA monohydrate (0.67 g, 0.1 eq.) was added and the resulting mixture was heated at reflux for 4 h. Afterwards, the mixture was allowed to cool to RT and the solvent was removed in vacuo. The residue was partitioned between water and DCM. The combined organic layers were dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (5.97 g, 70% yield) as a yellow solid.

ESI-MS: 241.1 [M+H]⁺. 1H NMR (400 MHz, DMSO-d₆) δ 7.90 (d, J=7.9 Hz, 1H), 3.62-3.56 (m, 2H), 2.68-2.61 (m, 3H), 0.88-0.82 (m, 2H), 0.72-0.67 (m, 2H).

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydro-1,8-naphthyridine-3-carbaldehyde

A solution of 7-chloro-1-cyclopropyl-6-fluoro-1,2,3,4-tetrahydro-1,8-naphthyridin-4-one (0.59 g, 1.0 eq.) in THF (5.0 mL) was added to a solution of 1 M NaHMDS in THF (0.54 g, 1.2 eq.) in THF (5.0 mL) at 0° C. The reaction mixture was stirred for 5 min at 0° C. and then, ethyl formate (0.26 mL, 1.3 eq.) was added, stirring was continued for 1 h. The reaction was quenched by addition of saturated aq. NH₄Cl solution (to pH neutral). The product was extracted into EtOAc. The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo. Additionally, the aq. layer was also concentrated in vacuo and the residue was rinsed with ethyl acetate. Both the crude residues were combined and dissolved in anh. MeOH (20.0 mL) and MnO₂ (1.073 g, 5.0 eq.) was added. After stirring this mixture at RT for 18 h, it was filtered through celite. The filter cake was flushed with DCM-methanol mixture (1:1) and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) and re-purified by two subsequent rounds of FCC (SiHP; DCM:MeOH) and (SIHP: 15 μm; Hex:EtOAc with 2% of DCM) to give the product (0.181 g, 28% yield) as an orange solid. ESI-MS: 268.1 [M+H]⁺.

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one (Intermediate 15)

A dry reactor vessel was charged with (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.31 g, 1.0 eq.), 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydro-1,8-naphthyridine-3-carbaldehyde (0.28 g, 1.0 eq.) and DCE (15.0 mL) and the resulting mixture was heated at 50° C. for 4 h. Then, the mixture was cooled to 0° C. and NaBH(OAc)₃ (0.45 g, 2.0 eq.) was added. The reaction was continued for 16 h at RT. Afterwards, water and sat. aq. solution of NaHCO₃ were added and the mixture was extracted with DCM. The combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.46 g, 75% yield) as a yellow solid. ESI-MS: 547.5 [M+H]⁺.

Preparation of 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one

A solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one (Intermediate 15) (0.1 g, 1.0 eq.) and NaN₃ (0.036 g, 3.0 eq.) in DMF (5.0 mL) was heated at 65° C. for 4 h. Then, the reaction mixture was allowed to cool to RT and was left stirring for 16 h. Afterwards, the reaction mixture was poured into 50.0 mL of cold water and the resulting mixture was washed with EtOAc. The organic layer was dried over anh. MgSO₄, filtered, concentrated in vacuo and dried. The residue was dissolved in EtOH (10.0 mL) and 10% Pd/C (0.011 g, 0.05 eq.) was added. The resulting mixture was stirred for 2 h under H₂ atmosphere. Then, the mixture was filtered through a pad of celite. The pad was washed with MeOH and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH with NH₃) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.039 g, 40% yield) as a white solid. ESI-MS: 528.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.17 (d, J=5.2 Hz, 1H), 8.08 (d, J=2.9 Hz, 1H), 7.87 (d, J=10.8 Hz, 1H), 7.79 (s, 1H), 7.34 (dd, J=8.6, 3.0 Hz, 1H), 7.25 (s, 1H), 7.24-7.18 (m, 1H), 7.11 (d, J=8.6 Hz, 1H), 3.90-3.82 (m, 3H), 3.76 (s, 2H), 3.64-3.56 (m, 1H), 3.53-3.45 (m, 1H), 3.00-2.91 (m, 1H), 2.90-2.80 (m, 1H), 2.75-2.65 (m, 1H), 2.41 (s, 3H), 2.38 (s, 3H), 2.16-2.09 (m, 1H), 1.96-1.88 (m, 1H), 1.75-1.58 (m, 2H), 1.20-1.11 (m, 2H), 0.86-0.79 (m, 2H).

Example 37. 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one Hydrochloride

DIPEA (0.157 mL, 2.0 eq.) was added to a mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one (Intermediate 15) (0.247 g, 1.0 eq.) and (3R)-pyrrolidin-3-ol (0.047 mL, 1.3 eq.) in MeCN (5.0 mL) at 25° C. The resulting mixture was heated for 16 h at 50° C. and subsequently partitioned between water and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN). The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.175 mL, 1.05 eq.) and a mixture of MeOH (21.0 mL) and H₂O (5.0 mL) as a solvent to provide the product (0.21 g, 99% yield) as a yellow solid. ESI-MS: 598.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.20 (d, J=5.2 Hz, 1H), 8.12 (d, J=2.9 Hz, 1H), 7.87 (d, J=13.2 Hz, 1H), 7.81 (s, 1H), 7.56-7.48 (m, 1H), 7.34-7.30 (m, 1H), 7.29-7.22 (m, 2H), 4.59-4.51 (m, 1H), 4.02-3.75 (m, 9H), 3.71-3.62 (m, 1H), 3.53-3.44 (m, 1H), 3.05-2.88 (m, 2H), 2.84-2.70 (m, 1H), 2.47 (s, 3H), 2.41 (s, 3H), 2.22-2.02 (m, 3H), 2.02-1.88 (m, 1H), 1.80-1.62 (m, 2H), 1.24-1.15 (m, 2H), 0.91-0.80 (m, 2H).

Example 38. 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A dry reactor vessel was charged with (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(6-nitropyridin-3-yl)piperidin-3-amine (Intermediate 11) (0.38 g, 1.0 eq.), 4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1)(0.2 g, 1.0 eq.) and a mixture of DCE and DMF (19.0 mL, v/v, 15/4). The reaction mixture was stirred for 2 h at 60° C. Then, the mixture was cooled to 0° C. and NaBH(OAc)₃ (0.49 g, 2.0 eq.) was added and the reaction was continued for 16 h at RT. Afterwards, water and sat. NaHCO₃ aq. solution were added and the resulting mixture was washed with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and repurified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.35 g, 60% yield) as a yellow solid. ESI-MS: 485.2 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.29-8.20 (m, 1H), 8.18 (d, J=5.4 Hz, 1H), 8.14-8.06 (m, 2H), 7.99 (s, 1H), 7.65 (ddd, J=8.4, 7.0, 1.5 Hz, 1H), 7.52-7.46 (m, 1H), 7.44-7.34 (m, 2H), 7.27 (d, J=8.5 Hz, 2H), 4.31-4.19 (m, 1H), 4.11-3.95 (m, 1H), 3.91-3.74 (m, 4H), 3.25-3.14 (m, 1H), 3.11-2.96 (m, 1H), 2.97-2.79 (m, 1H), 2.37 (s, 3H), 2.23-2.10 (m, 1H), 1.99-1.73 (m, 2H), 1.71-1.50 (m, 1H).

Preparation of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

10% Pd/C (0.03 g, 0.1 eq.) was added to a solution of the 3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(6-nitropyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.13 g, 1.0 eq.) in EtOH (10.0 mL). The resulting mixture was stirred under H₂ atmosphere for 4 h. Then, the reaction mixture was filtered through a pad of celite. The pad was washed with MeOH and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:TFA). The sample was basified with saturated aq. solution of NaHCO₃ and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.06 g, 48% yield) as a white solid. ESI-MS: 455.7 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.26 (ddd, J=8.3, 1.5, 0.6 Hz, 1H), 8.19 (dd, J=5.1, 0.8 Hz, 1H), 8.02 (s, 1H), 7.69-7.63 (m, 2H), 7.53-7.49 (m, 1H), 7.39 (ddd, J=8.1, 6.9, 1.1 Hz, 1H), 7.34 (dd, J=8.9, 2.9 Hz, 1H), 7.29-7.25 (m, 2H), 6.59-6.56 (m, 1H), 3.88-3.78 (m, 4H), 3.63-3.56 (m, 1H), 3.29-3.26 (m, 1H), 3.02-2.92 (m, 1H), 2.77-2.69 (m, 1H), 2.59-2.50 (m, 1H), 2.39 (s, 3H), 2.16-2.08 (m, 1H), 1.94-1.86 (m, 1H), 1.73-1.52 (m, 2H).

Example 39. 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.3 g, 1.0 eq.), (3S)-pyrrolidin-3-ol hydrochloride (0.136 g, 2.0 eq.), Cs₂CO₃ (0.38 g, 2.1 eq.) and DMF (3.0 mL). The resulting mixture was purged with argon for 5 min. Subsequently, BINAP (0.034 g, 0.1 eq.) and Pd₂(dba)₃ (0.028 g, 0.05 eq.) were added, the vessel was closed and the resulting mixture was stirred overnight at 115° C. Then, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by two consecutive FCCs (SiHP; DCM:MeOH) and (SiHP, 15 μm; DCM:MeOH) followed by RP-FCC (H₂O:MeCN) and re-purified by prep-HPLC (H₂O:MeCN:NH₃). After trituration with Et₂O the precipitate was filtered off and dried in vacuo. The isolated sample was converted into the HCl salt using 2 M HCl in Et₂O (0.042 mL, 1.0 eq. to FB) and a mixture of MeOH (20.0 mL) and H₂O (5.0 mL) as the solvent to give the product (0.053 g, 15% yield) as a yellow solid. ESI-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.31 (d, J=5.8 Hz, 1H), 8.26 (d, J=3.0 Hz, 1H), 8.07-8.02 (m, 1H), 7.88 (s, 1H), 7.75 (d, J=14.6 Hz, 1H), 7.70-7.59 (m, 3H), 6.94 (d, J=7.7 Hz, 1H), 4.60-4.54 (m, 1H), 4.24-4.12 (m, 3H), 4.07-3.74 (m, 5H), 3.69-3.61 (m, 1H), 3.58-3.53 (m, 1H), 3.48-3.40 (m, 1H), 3.23-3.08 (m, 2H), 2.98-2.88 (m, 1H), 2.62 (s, 3H), 2.50 (s, 3H), 2.29-1.96 (m, 4H), 1.89-1.66 (m, 2H), 1.37-1.27 (m, 2H), 1.02-0.94 (m, 2H).

Example 40. 3-({[(3S)-1-[6-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

Preparation of (5-bromopyridin-2-yl)methanol

NaBH₄ (0.82 g, 2.5 eq.) was added in portions to a solution of methyl 5-bromopyridine-2-carboxylate (1.87 g, 1.0 eq.) in anh. MeOH (20.0 mL) cooled to 0° C. The resulting mixture was stirred overnight at RT under argon atmosphere. Then, the reaction mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the product (1.6 g, 98% yield) as a colorless oil. ESI-MS: 188.0 [M+H]⁺.

Preparation of (5-bromopyridin-2-yl)methyl Acetate

Ac₂O (2.0 mL, 2.5 eq.) was added to a solution of (5-bromopyridin-2-yl)methanol (1.6 g, 1.0 eq.) and TEA (4.74 mL, 4.0 eq.) in anh. DCM (20.0 mL). The resulting mixture was stirred for 26 h at RT under argon atmosphere. Then, the reaction mixture was partitioned between NaOH aq. solution and DCM. The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by two consecutive FCCs (SiHP; Hex:EtOAc) to give the product (1.5 g, 77% yield) as a yellow solid. ESI-MS: 230.1 [M+H]⁺.

Preparation of {5-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-1-yl]pyridin-2-yl}methyl Acetate

A mixture of tert-butyl N-[(3S)-piperidin-3-yl]carbamate (1.12 g, 1.3 eq.), (5-bromopyridin-2-yl)methyl acetate (1.0 g, 1.0 eq.), Cs₂CO₃ (2.8 g, 2 eq.) and anh. 1,4-dioxane (20.0 mL) was purged with argon for 10 min. Then, Pd₂(dba)₃ (0.197 g, 0.05 eq.) and Xantphos (0.149 g, 0.06 eq.) were added under inert atmosphere and the resulting mixture was again purged with argon for another 10 min. Then, the mixture was stirred for 24 h at 120° C. Subsequently, the reaction mixture was cooled to ambient temperature, filtered through a pad of celite and concentrated under reduced pressure. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.98 g, 61% yield) as a dark brown oil. ESI-MS: 350.3 [M+H]⁺.

Preparation of {5-[(3S)-3-aminopiperidin-1-yl]pyridin-2-yl}methanol

To a solution of {5-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-1-yl]pyridin-2-yl}methyl acetate (0.98 g, 1.0 eq.) in 1,4-dioxane (7.0 mL) was added 4M HCl in 1,4-dioxane solution (3.3 g, 5.0 eq.). The resulting mixture was stirred for 1 h at 60° C. Then, the reaction mixture was concentrated in vacuo. The residue was partitioned between NaOH aq. solution and a mixture of CHCl₃ and iPrOH (4/1). The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give product (0.46 g, 74% yield) as a dark brown oil. ESI-MS: 208.1 [M+H]⁺.

Preparation of 3-({[(3S)-1-[6-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

A solution of 1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 7) (0.33 g, 1.0 eq.), NaOAc (0.14 g, 1.0 eq.) and {5-[(3S)-3-aminopiperidin-1-yl]pyridin-2-yl}methanol (0.46 g, 1.1 eq.) in anh. MeOH (10.0 mL) was stirred overnight at RT. Then, NaBH₄ (0.09 g, 1.3 eq.) was added and the resulting mixture was stirred for 1 h at RT. Afterwards, the reaction mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.34 g, 51% yield) as a white solid. ESI-MS: 379.6 [M+H]⁺.

Preparation of 3-({[(3S)-1-[6-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

A mixture of 2-methylpyridine-4-carbaldehyde (0.07 g, 1.2 eq.) and 3-({[(3S)-1-[6-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one (0.2 g, 1.0 eq.) in DCE (5.0 mL) was stirred for 2 h at 60° C. Then, NaBH(OAc)₃ (0.28 g, 2.5 eq.) was added and the resulting mixture was stirred for an additional 24 h at 60° C. Subsequently, the reaction mixture was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.088 g, 34% yield) as a white solid. ESI-MS: 484.9 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.31-8.27 (m, 1H), 8.22-8.17 (m, 2H), 8.04 (s, 1H), 7.72 (ddd, 1=8.6, 6.9, 1.7 Hz, 1H), 7.63 (d, J=8.4 Hz, 1H), 7.38 (ddd, J=8.0, 6.9, 1.0 Hz, 1H), 7.32 (dd, J=8.7, 2.9 Hz, 1H), 7.27-7.20 (m, 3H), 5.15 (t, J=5.8 Hz, 1H), 4.43 (d, J=5.8 Hz, 2H), 3.96-3.88 (m, 1H), 3.86 (s, 3H), 3.82-3.55 (m, 5H), 2.83 (t, J=11.3 Hz, 1H), 2.78-2.58 (m, 2H), 2.38 (s, 3H), 2.07-1.95 (m, 1H), 1.82-1.71 (m, 1H), 1.63-1.39 (m, 2H).

Example 41. 1-(2-hydroxyethyl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-(2-hydroxyethyl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1)(0.5 g, 1.0 eq.) was suspended in anh. DMF (20.0 mL) and K₂CO₃ (1.12 g, 2.8 eq.) was added. The resulting mixture was stirred for 10 min at RT and then KI (1.3 g, 2.8 eq.) and 2-bromoethanol (0.57 mL, 2.8 eq.) were added sequentially. The reaction mixture was stirred at 90° C. for 24 h. Subsequently the mixture was filtered through a funnel and the residue was washed with DCM. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by FCC (SiHP; DCM:EtOAc) to give the product (0.16 g, 25% yield) as a beige solid. ESI-MS: 218.2 [M+H]⁺.

Preparation of 2-(3-formyl-4-oxo-1,4-dihydroquinolin-1-yl)ethyl Acetate

Acetic anhydride (0.1 mL, 2.3 eq.) was added to a mixture of 1-(2-hydroxyethyl)-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.1 g, 1.0 eq.), DMAP (0.003 g, 0.05 eq.) and pyridine (2.0 mL) placed in a cooling bath and the mixture was stirred at RT for 2.5 h. The mixture was diluted with sat. NaHCO₃ aq. solution and the product was extracted into ethyl acetate. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.082 g, 69% yield) as a white solid. ESI-MS: 260.3 [M+H]⁺.

Preparation of 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]ethyl Acetate

A mixture of 2-(3-formyl-4-oxo-1,4-dihydroquinolin-1-yl)ethyl acetate (0.082 g, 1.0 eq.), (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.11 g, 1.1 eq.) and DCE (5.0 mL) was heated at 50° C. for 1 h and then left stirring overnight at RT. Subsequently, NaBH(OAc)₃ (0.15 g, 2.2 eq.) was added and the resulting mixture was stirred for 1.5 h at RT. Then, the mixture was partitioned between water and DCM. The organic layer was washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FFC (SiHP; DCM:MeOH) to give the product (0.103 g, 61% yield) as a yellow solid. ESI-MS: 540.1 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.1 Hz, 1H), 8.20 (dd, J=8.1, 1.6 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 8.02 (s, 1H), 7.77 (d, J=8.6 Hz, 1H), 7.70 (ddd, J=8.6, 6.8, 1.7 Hz, 1H), 7.36 (ddd, J=7.9, 6.8, 1.0 Hz, 1H), 7.30-7.23 (m, 2H), 7.20 (dd, J=8.5, 3.0 Hz, 1H), 7.01 (d, J=8.5 Hz, 1H), 4.58 (t, J=5.2 Hz, 2H), 4.33 (t, J=5.2 Hz, 2H), 3.89-3.52 (m, 6H), 2.81-2.69 (m, 2H), 2.61-2.53 (m, 1H), 2.39 (s, 3H), 2.32 (s, 3H), 2.04-1.94 (m, 1H), 1.77 (s, 3H), 1.76-1.71 (m, 1H), 1.59-1.36 (m, 2H).

Preparation of 1-(2-hydroxyethyl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]ethyl acetate (0.097 g, 1.0 eq.), LiOH*H₂O (0.038 g, 5.0 eq.), methanol (5.0 mL) and H₂O (1.0 mL) was stirred overnight at RT. Then, the mixture was diluted with water and sat. NaHCO₃ aq. solution and the product was extracted into DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.047 g, 52% yield) as a light yellow solid. ESI-MS: 498.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.0 Hz, 1H), 8.20 (dd, J=8.1, 1.6 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 8.03 (s, 1H), 7.73 (d, J=8.6 Hz, 1H), 7.67 (ddd, J=8.6, 6.7, 1.7 Hz, 1H), 7.34 (ddd, J=8.0, 6.8, 1.0 Hz, 1H), 7.27 (s, 1H), 7.24 (d, J=5.0 Hz, 1H), 7.20 (dd, J=8.5, 3.0 Hz, 1H), 7.00 (d, J=8.5 Hz, 1H), 4.99 (t, J=5.3 Hz, 1H), 4.43-4.26 (m, 2H), 3.88-3.69 (m, 5H), 3.66-3.54 (m, 3H), 2.80-2.67 (m, 2H), 2.62-2.53 (m, 1H), 2.39 (s, 3H), 2.31 (s, 3H), 2.05-1.91 (m, 1H), 1.81-1.67 (m, 1H), 1.59-1.38 (m, 2H).

Example 42. 6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one

Preparation of ethyl 2-(2,6-dichloro-5-fluoropyridine-3-carbonyl)-3-(dimethylamino)prop-2-enoate

2,6-dichloro-5-fluoropyridine-3-carboxylic acid (5.4 g, 1.0 eq.) was suspended in SOCl₂ (9.4 mL, 5.0 eq.) and the resulting mixture was stirred at 80° C. for 2 h. During that time the solution became clear. Then, the mixture was concentrated with the co-solvent DCM to dryness. The residue was dissolved in toluene (20.0 mL) and the resulting solution was added over 5 min to a stirring mixture of ethyl 3-(N,N-dimethylamino)acrylate (3.7 mL, 1.0 eq.) and DIPEA (9.45 mL, 2.1 eq.) at RT. The reaction mixture was stirred at RT for 15 min and then heated at 90° C. for 3.5 h. Afterwards, the mixture was partitioned between DCM and water. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (2.8 g, 32% yield) as an orange oil. ESI-MS: 335.0 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.04 (d, J=7.9 Hz, 1H), 7.95 (s, 1H), 3.91 (q, J=7.1 Hz, 2H), 3.39 (s, 3H), 2.93 (s, 3H), 0.94 (t, J=7.1 Hz, 3H).

Preparation of ethyl 2-(2,6-dichloro-5-fluoropyridine-3-carbonyl)-3-[(propan-2-yl)amino]prop-2-enoate

Isopropylamine (0.3 mL, 1.1 eq.) was added to a solution of ethyl 2-(2,6-dichloro-5-fluoropyridine-3-carbonyl)-3-(dimethylamino)prop-2-enoate (1.05 g, 1.0 eq.) in EtOH:Et₂O mixture (8:3) (22.0 mL). The reaction mixture was stirred at RT for 15 min. Then, solvents were evaporated in vacuo affording the product (1.18 g, 99% yield) as an orange oil which was used in the next step without further purification. ESI-MS: 349.0 [M+H]+

Preparation of ethyl 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylate

A solution of ethyl 2-(2,6-dichloro-5-fluoropyridine-3-carbonyl)-3-[(propan-2-yl)amino]prop-2-enoate (0.85 g, 1.0 eq.) in MeCN (8.0 mL) was treated with K₂CO₃ (0.53 g, 1.6 eq.) and the resulting mixture was stirred for 2 h at 80° C. Afterwards, the reaction was quenched by the addition of water. Then, the reaction mixture was mixed with three other crude mixtures of trial reactions set up using different solvents (MeCN, NMP and DMAc) using identical conditions but starting from a smaller amount of ethyl 2-(2,6-dichloro-5-fluoropyridine-3-carbonyl)-3-[(propan-2-yl)amino]prop-2-enoate (0.1 g, 0.3 mmol). The resulting mixture was washed with DCM and the combined organic layers were dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was dissolved in 5.0 mL of DCM and heptane was added. The precipitate was filtered off and dried in vacuo affording ethyl 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylate (0.81 g, 85% yield) as an off-white solid. ESI-MS: 313.9 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.71 (s, 1H), 8.48 (d, J=7.9 Hz, 1H), 5.53-5.43 (m, 1H), 4.26 (q, J=7.1 Hz, 2H), 1.50 (s, 3H), 1.49 (s, 3H), 1.29 (t, J=7.1 Hz, 3H).

Preparation of 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylic Acid

A mixture of ethyl 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylate (0.81 g, 1.0 eq.), conc. HCl (2.0 mL), H₂O (6.0 mL) and 1,4-dioxane (12.0 mL) was stirred for 4 h at 100° C. Subsequently, the reaction mixture was cooled to RT, the precipitate was filtered off and dried to give the product (0.67 g, 90% yield) as an off-white solid which was used in the next step without further purification. ESI-MS: 285.0 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 14.40 (s, 1H), 9.01 (s, 1H), 8.73 (d, J=7.6 Hz, 1H), 5.61 (hept, J=6.4 Hz, 1H), 1.56 (d, J=6.7 Hz, 6H).

Preparation of 7-chloro-6-fluoro-1-(propan-2-yl)-1,2,3,4-tetrahydro-1,8-naphthyridin-4-one

NaBH₄ (0.40 g, 4.5 eq.) was added slowly to a solution of 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylic acid (0.67 g, 1.0 eq.) in anh. MeOH (10.0 mL) stirring under inert atmosphere. Afterwards, the mixture was allowed to warm to RT and PTSA monohydrate (0.045 g, 0.1 eq.) was added. After heating at reflux for 1.5 h, the mixture was allowed to cool to RT and the solvent was removed in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.46 g, 81% yield) as a yellow solid. ESI-MS: 243.1 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 7.88 (d, J=8.0 Hz, 1H), 4.88 (hept, J=6.7 Hz, 1H), 3.52-3.44 (m, 2H), 2.67-2.59 (m, 2H), 1.17 (d, J=6.8 Hz, 6H).

Preparation of 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carbaldehyde

7-Chloro-6-fluoro-1-(propan-2-yl)-1,2,3,4-tetrahydro-1,8-naphthyridin-4-one (0.46 g, 1.0 eq.) dissolved in anh. THF (2.0 mL) was added to a solution of NaH MDS (2 M in THF, 1.14 mL, 1.2 eq.) in anh. THF (2.0 mL) at 0° C. The reaction mixture was stirred for 30 min at the same temperature and subsequently a solution of ethyl formate (0.2 mL, 1.3 eq.) in THF (2.0 mL) was added. The mixture was stirred for 1 h, then the reaction was quenched by addition of sat. NH₄Cl aq. solution and the mixture was washed with EtOAc. The aqueous layer was washed further with EtOAc (×3). The combined organic layers were dried over anh. Na₂SO₄, filtered, concentrated and dried in vacuo. The residue was dissolved in anh. 1,4-dioxane (10.0 mL) and MnO₂ (0.82 g, 4.2 eq.) was added. The resulting mixture was heated at 50° C. for 16 h. Afterwards, the reaction mixture was cooled to RT, filtered through a pad of celite and the pad was washed with a mixture of DCM:MeOH (v/v, 7/3). The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:EtOAc) to give the product (0.291 g, 57% yield) as a yellow solid. APMS: 269.0 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.14 (s, 1H), 8.68 (s, 1H), 8.58 (d, J=7.7 Hz, 1H), 5.51 (hept, J=6.7 Hz, 1H), 1.52 (d, J=6.8 Hz, 6H).

Preparation of 7-chloro-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one

A dry reactor vessel was charged with (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.31 g, 1.02 eq.), 7-chloro-6-fluoro-4-oxo-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carbaldehyde (0.29 g, 1.0 eq.) and DCE (10.0 mL). The mixture was heated for 4 h at 50° C. and then cooled to 0° C. Then, NaBH(OAc)₃ (0.43 g, 2.0 eq.) was added and the resulting mixture was left stirring for 16 h at RT. Subsequently, water and sat. NaHCO₃ aq. solution were added and the mixture was washed with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH). The isolated material was additionally re-purified by FCC (SiHP; DCM:MeOH with NH₃) and RP-FCC (C18HP; H₂O:MeCN) to give the product (0.40 g, 70% yield) as a white solid. ESI-MS: 549.4 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.42-8.34 (m, 1H), 8.24-8.15 (m, 2H), 8.06 (d, J=3.0 Hz, 1H), 7.34 (dd, J=8.6, 3.0 Hz, 1H), 7.30 (s, 1H), 7.27-7.22 (m, 1H), 7.11 (d, J=8.6 Hz, 1H), 5.75-5.59 (m, 1H), 3.96-3.76 (m, 5H), 3.64-3.53 (m, 1H), 3.02-2.92 (m, 1H), 2.90-2.82 (m, 1H), 2.77-2.64 (m, 1H), 2.48-2.35 (m, 6H), 2.22-2.07 (m, 1H), 2.00-1.85 (m, 1H), 1.77-1.57 (m, 2H), 1.53-1.36 (m, 6H).

Preparation of 6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one

A mixture of 7-chloro-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one (0.15 g, 1.0 eq.) and (3R)-pyrrolidin-3-ol (0.028 mL, 1.3 eq.) in MeCN (5.0 mL) at 25° C. was treated with DIPEA (0.093 mL, 2.0 eq.). The reaction mixture was heated for 18 h at 50° C. Then, water was added and the resulting mixture was washed with DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (C18HP; H₂O:MeCN) to give the product (0.16 g, 95% yield) as a yellow solid. ESI-MS: 600.8 [M+H]⁺. 1H NMR (400 MHz, Methanol-d₄) δ 8.19 (d, J=5.3 Hz, 1H), 8.07 (d, J=2.9 Hz, 1H), 7.94 (s, 1H), 7.89 (d, J=13.2 Hz, 1H), 7.37-7.28 (m, 2H), 7.27-7.22 (m, 1H), 7.10 (d, J=8.6 Hz, 1H), 5.72-5.57 (m, 1H), 4.56-4.46 (m, 1H), 3.99-3.73 (m, 9H), 3.65-3.54 (m, 1H), 3.01-2.89 (m, 1H), 2.90-2.78 (m, 1H), 2.73-2.62 (m, 1H), 2.41 (s, 3H), 2.40 (s, 3H), 2.18-1.99 (m, 3H), 1.96-1.85 (m, 1H), 1.76-1.59 (m, 2H), 1.43 (d, J=6.8 Hz, 6H).

Example 43. 3-({[(3S)-1-[6-(2-hydroxyethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

Preparation 2-(5-bromopyridin-2-yl)ethan-1-ol

A two-necked round-bottomed flask equipped with thermometer was charged with anh. THF (2.0 mL) and cooled to −78° C. Then, LDA (2 M in THF/heptane/ethylbenzene, 1.74 mL, 1.5 eq.) was added dropwise at −78° C. The resulting mixture was stirred for 15 min and then solution of 5-bromo-2-methylpyridine (0.4 g, 1.0 eq.) in anh. THF (4.0 mL) was dropped in slowly at −78° C. and the reaction mixture was stirred for 1.5 h at −78° C. Afterwards, DMF (1.8 mL, 10.0 eq.) was added dropwise and stirring was continued for 1 h at −78° C. Subsequently, the mixture was allowed to warm to RT and MeOH (4.5 mL) was added followed by NaBH₄ (0.089 g, 1.0 eq.). During NaBH₄ addition generation of heat was observed, temperature increased to 30° C. The reaction was continued for 30 min at RT and then quenched with saturated NH₄Cl aq. solution. The resulting mixture was extracted with EtOAc (3×). The combined organic layers were washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by two consecutive FCC (SiHP; DCM:MeOH) and (SiHP; hexane:EtOAc) to give the product (0.204 g, 35% yield) as a yellowish oil. ESI-MS: 202.0 [M+H]⁺.

Preparation of 2-(5-bromopyridin-2-yl)ethyl Acetate

2-(5-bromopyridin-2-yl)ethan-1-ol (0.17 g, 1.0 eq.) was placed into an oven-dried flask flushed with argon, and anh. DCM (4.0 mL) and TEA (0.19 mL, 2.0 eq.) were sequentially added. The reaction mixture was cooled in an ice-water bath and stirred for 15 min, and then acetyl chloride (0.07 mL, 1.5 eq.) was added dropwise. The reaction mixture was stirred overnight at RT and quenched with sat. NaHCO₃ solution and extracted with DCM (3×). The combined organic layers were washed with water and brine, then dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP, Hex:EtOAc) to give the desired product (0.19 g, quantitative yield) as a yellow liquid. ESI-MS: 244.1, 245.8 [M+H]⁺.

Preparation 2-{5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}ethyl Acetate

1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5)(0.12 g, 1.0 eq.), 2-(5-bromopyridin-2-yl)ethyl acetate (0.09 g, 1.1 eq.), Cs₂CO₃ (0.14 g, 1.4 eq.) and anh. 1,4-dioxane (3.0 mL) were placed into a pressure vessel. The resulting mixture was purged with argon for 5 min, Pd₂(dba)₃ (0.014 g, 0.05 eq.) and Xantphos (0.018 g, 0.1 eq.) were then added and the reaction mixture was stirred at 115° C. overnight. After cooling to room temperature, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) to give a product (0.049 g, 28% yield) as a yellow solid. ESI-MS: 540.4 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.28 (d, J=5.1 Hz, 1H), 8.23-8.15 (m, 2H), 8.03 (s, 1H), 7.76-7.67 (m, 1H), 7.65-7.58 (m, 1H), 7.41-7.33 (m, 1H), 7.28-7.21 (m, 3H), 7.07 (d, J=8.6 Hz, 1H), 4.28 (t, J=6.9 Hz, 2H), 3.94-3.83 (m, 4H), 3.82-3.71 (m, 2H), 3.70-3.54 (m, 3H), 2.88 (t, J=6.7 Hz, 2H), 2.83-2.55 (m, 3H), 2.37 (s, 2H), 2.05-1.91 (m, 4H), 1.81-1.69 (m, 1H), 1.64-1.38 (m, 2H), 1.36-1.20 (m, 1H).

Preparation of 3-({[(3S)-1-[6-(2-hydroxyethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

2-{5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}ethyl acetate (0.05 g, 1.0 eq.) was dissolved in MeOH (2.5 mL), then H₂O (1.0 mL) was added followed by the addition of LiOH xH₂O (0.018 g, 4.9 eq.). The reaction mixture was stirred for 3 h at RT, then concentrated to dryness and partitioned between H₂O and DCM. The aqueous phase was basified with 15 M NaOH topH≈12 and extracted with DCM (3×). The combined organic layers were dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.025 g, 57% yield) as a white solid. ESI-MS: 498.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ8.28 (d, J=5.0 Hz, 1H), 8.21-8.17 (m, 2H), 8.02 (s, 1H), 7.74-7.69 (m, 1H), 7.62 (d, J=8.6 Hz, 1H), 7.40-7.34 (m, 1H), 7.26-7.20 (m, 3H), 7.04 (d, J=8.5 Hz, 1H), 4.57 (t, J=5.3 Hz, 1H), 3.90-3.83 (m, 4H), 3.73 (q, 2H), 3.68-3.55 (m, 5H), 2.79 (t, J=11.1 Hz, 1H), 2.76-2.69 (m, 3H), 2.64-2.55 (m, 1H), 2.37 (s, 3H), 2.03-1.96 (m, 1H), 1.79-1.72 (m, 1H), 1.59-1.39 (m, 2H).

Example 44. 3-({[(3S)-1-[5-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

Preparation of (5-bromopyridin-3-yl)methyl Acetate

5-bromo-3-pyridinemethanol (0.50 g, 1.0 eq.) was suspended in DCM (3.0 mL) and trimethylamine (0.74 mL, 2.0 eq.) was added followed by the addition of acetyl chloride (0.47 g, 1.5 eq.).

The reaction mixture was stirred at RT overnight. After that, the reaction mixture was diluted with DCM and washed with aq. sol. NaHCO₃, brine and dried over anh. Na₂SO₄. Crude product was purified by FCC (SiHP, Hex:EtOAc) provide to product (0.56 g, 82% yield) as a colorless liquid. ESI-MS: 231.1 [M+2]⁺.

Preparation of {5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-3-yl}methyl Acetate

1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.15 g, 1.0 eq.), (5-bromopyridin-3-yl)methyl acetate (0.10 g, 1.1 eq.), cesium carbonate (0.18 g, 1.4 eq.) and 1,4-dioxane (3.0 mL) were placed in a pressure vessel. The resulting mixture was purged with argon for 5 min, then Pd₂(dba)₃ (0.018 g, 0.1 eq.) and Xantphos (0.023 g, 0.1 eq.) were added. The reaction mixture was stirred at 115° C. overnight, filtered through a pad of celite and concentrated under reduced pressure. The crude mixture was purified by FCC (SiHP, DCM:MeOH) give of product (0.133 g, 57% yield) as a yellow solid. ESI-MS: 526.5 [M+H]⁺.

Preparation of 3-({[(3S)-1-[5-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

{5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-3-yl}methyl acetate (0.13 g, 1.0 eq.) was suspended in water (1.0 mL) and methanol (5.0 mL). Then lithium hydroxide monohydrate (0.03 g, 5.0 eq.) was added and the reaction mixture was stirred at RT overnight. The reaction mixture was concentrated under reduced pressure and the residue was partitioned between DCM and 15 M NaOH aq. solution. Separated organic layer was dried over anh. Na₂SO₄ and concentrated. The residue was purified by RP-FCC (C18HP, H₂O:MeCN) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) give a product (0.043 g, 40% yield) as white powder. ESI-MS: 484.4 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) 8.35 (dd, J=8.3, 1.5 Hz, 1H), 8.19-8.15 (m, 2H), 8.02 (s, 1H), 7.93-7.90 (m, 1H), 7.81-7.75 (m, 1H), 7.67 (d, J=8.6 Hz, 1H), 7.50-7.44 (m, 1H), 7.42-7.37 (m, 1H), 7.29-7.24 (m, 2H), 4.60 (s, 2H), 4.07-3.97 (m, 1H), 3.93-3.81 (m, 7H), 3.77-3.71 (m, 1H), 3.01-2.92 (m, 2H), 2.82-2.74 (m, 1H), 2.33 (s, 3H), 2.22-2.15 (m, 1H), 1.96-1.90 (m, 1H), 1.69 (d, J=7.5 Hz, 2H).

Example 45. 1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of ethyl (2Z)-2-[(Z)-4-bromo-2-fluorobenzoyl]-3-(dimethylamino)prop-2-enoate

A mixture of ethyl (2E)-3-(dimethylamino)prop-2-enoate (0.60 g, 1.0 eq.) and N,N-diisopropylethylamine (1.1 g, 2.1 eq.) was stirred at RT and the solution of 4-bromo-2-fluorobenzoyl chloride (1.0 g, 1.0 eq.) in toluene (5.0 mL) was added over 5 min. The yellow solution that formed was heated in an oil bath at 90° C. After 3 h, the mixture was diluted with DCM/water. The separated organic layer was dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP, EtOAc) to give the product (1.24 g, 81% yield) as a yellow oil. ESI-MS: 344.0 [M+H]⁺.

Preparation of ethyl 7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carboxylate

Ethyl (2Z)-2-[(2)-4-bromo-2-fluorobenzoyl]-3-(dimethylamino)prop-2-enoate (1.24 g, 1.0 eq.) and cyclopropylamine (0.27 g, 1.3 eq.) were heated in toluene (10.0 mL) at 110° C. for 2 h. The reaction mixture was concentrated and the residue was diluted in DMF (8.0 mL). Then K₂CO₃ (1.25 g, 2.5 eq.) was added and the reaction mixture was heated at 100° C. overnight. After cooling, the reaction mixture was diluted with DCM, washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP, Hex:EtOAc) give the product (0.97 g, 88% yield) as a yellow solid. ESI-MS: 337.2 [M+2]+.

Preparation of 7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carboxylic Acid

Ethyl 7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carboxylate was suspended (0.95 g, 1.0 eq.) in 1 M aq. HCl (8.0 mL) and the mixture was stirred at 95° C. overnight. The reaction mixture was concentrated in vacuo. Then, the residue was further dissolved in toluene and traces of moisture were removed as an azeotropic mixture to give the product (0.80 g, 92% yield) as a white solid. ESI-MS: 309.9 [M+2]⁺.

Preparation of 7-bromo-1-cyclopropyl-1,2,3,4-tetrahydroquinolin-4-one

7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (0.80 g, 1.0 eq.) was dissolved in MeOH (10.0 mL) and the mixture was cooled in an ice-bath. Then sodium borohydride (0.51 g, 4.5 eq.) was added in portions and the reaction mixture was stirred at RT overnight. Then, PTSA monohydrate (0.06 g, 0.1 eq.) was added and stirring was continued at reflux for 3 h. The reaction mixture was concentrated in vacuo, then diluted with DCM, washed with water, brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The crude product mixture was purified by FCC (SiHP, Hex:EtOAc) to give the product (0.52 g, 66% yield) as a yellow solid. ESI-MS: 266.0 [M+H], 267.9 [M+2+H]⁺.

Preparation of 7-bromo-1-cyclopropyl-4-oxo-1,2,3,4-tetrahydroquinoline-3-carbaldehyde

A solution of 7-bromo-1-cyclopropyl-1,2,3,4-tetrahydroquinolin-4-one (0.40 g, 1.0 eq.) in DCM (10.0 mL) was added dropwise to a viscous mixture of ethyl formate (0.48 mL, 4.0 eq.) and sodium methoxide (0.32 g, 4.0 eq.). The reaction mixture was stirred at RT for 3 hours and subsequently quenched with ice-water. After separation, the organic layer was washed with 2 M NaOH. The aqueous layer was acidified to pH=6 with conc. hydrochloric acid and then washed with DCM. The organic extract was dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the product (0.44 g, 91% yield) as an orange solid. ESI-MS: 293.9 [M+H]⁺.

Preparation of 7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 16)

A mixture of 7-bromo-1-cyclopropyl-4-oxo-1,2,3,4-tetrahydroquinoline-3-carbaldehyde (0.44 g, 1.0 eq.) and manganese (IV) oxide (0.59 g, 5.0 eq.) in MeOH (10.0 mL) was stirred at RT for 2 days. The reaction mixture was filtered through a pad of celite. The pad was washed with DCM:MeOH (4:1) and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH). The purified product was triturated by MeOH to give product (0.17 g, 41% yield) as a yellow solid. ESI-MS: 293.2 [M+2]⁺.

Preparation of 7-bromo-1-cyclopropyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

7-bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 16) (0.13 g, 1.0 eq.) and (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.14 g, 1.0 eq.) were suspended in anh. DCE (5.0 mL). The resulting mixture was stirred at RT for 30 min, then sodium triacetoxyborohydride (0.26 g, 2.8 eq.) was added and stirring was continued overnight. The reaction mixture was poured into ice-cold water, then NaHCO₃ was added. to the mixture was then washed with DCM (3×). The combined organic layers were dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by RP-FCC (C18HP, H₂O:MeCN) give the product (0.18 g, 68% yield) as white solid. ESI-MS: 574.1 [M+2+H]⁺.

Preparation of 1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 7-bromo-1-cyclopropyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.1 g, 1.0 eq.), (R)-Pyrrolidin-3-ol hydrochloride (0.022 g, 1.5 eq.), cesium carbonate (0.098 g, 1.8 eq.) and 1,4-dioxane (2.0 mL) was taken in a pressure vessel. The resulting mixture was purged with argon for 5 min, then BINAP (0.021 g, 0.2 eq.) and Pd₂(dba)₃ (0.015 g, 0.1 eq.) were added and the reaction mixture was stirred at 95° C. for 16 h. The reaction was cooled and filtered through a short pad of celite and concentrated in vacuo. The residue was suspended in DCM, washed with water, brine and dried over anh. Na₂SO₄. The crude product mixture was purified by FCC (SiHP, MeOH:DCM), then repurified by prep-HPLC (H₂O:MeCN:NH₃) and RP-FCC (C18HP; H₂O:MeCN) to provide the product (0.017 g, 17% yield) as a yellow solid. ESI-MS: 579.6 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.17 (d, J=5.2 Hz, 1H), 8.13 (d, J=9.1 Hz, 1H), 8.10 (d, J=2.9 Hz, 1H), 7.81 (s, 1H), 7.36 (dd, J=8.6, 3.0 Hz, 1H), 7.26 (s, 1H), 7.24-7.22 (m, 1H), 7.12 (d, J=8.6 Hz, 1H), 6.83 (dd, J=9.1, 2.2 Hz, 1H), 6.77 (d, J=2.2 Hz, 1H), 4.63-4.56 (m, 1H), 3.92-3.77 (m, 5H), 3.66-3.57 (m, 3H), 3.56-3.49 (m, 1H), 3.41-3.35 (m, 2H), 3.03-2.94 (m, 1H), 2.86 (t, J=11.1 Hz, 1H), 2.76-2.64 (m, 1H), 2.42 (s, 3H), 2.35 (s, 3H), 2.28-2.08 (m, 3H), 1.99-1.86 (m, 1H), 1.76-1.60 (m, 2H), 1.34-1.21 (m, 3H), 0.95-0.84 (m, 2H).

Example 46. 1-[1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylic Acid

Preparation of red-butyl N-[(3S)-1-(pyridin-3-yl)piperidin-3-yl]carbamate

A pressure vessel was charged with 3-bromopyridine (3.0 g, 1.0 eq.), tert-butyl N-[(3S)-piperidin-3-yl]carbamate (4.9 g, 1.3 eq.), Cs₂CO₃ (8.35 g, 1.4 eq.) and 1,4-dioxane (80.0 mL). The resulting mixture was purged with argon for 15 min. Then, Pd₂(dba)₃ (0.87 g, 0.05 eq.) and Xantphos (0.66 g, 0.06 eq.) were added, the vessel was sealed and the reaction mixture was stirred for 4 days at 115° C. Subsequently, the mixture was filtered through a pad of celite, the pad was washed with DCM and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:EtOAc) to give the product (3.8 g, 69% yield) as a yellow oil. ESI-MS: 278.4 [M+H]⁺.

Preparation of (3S)-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 17)

4 M HCl in 1,4-dioxane (20.0 mL) was added to a suspension of tert-butyl N-[(3S)-1-(pyridin-3-yl)piperidin-3-yl]carbamate (3.76 g, 1.0 eq.) in 1,4-dioxane (20.0 mL) and the resulting mixture was stirred for 1 h at 55° C. Then, the reaction mixture was concentrated in vacuo and the residue was partitioned between 15% NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the crude product (2.3 g, 92% yield) as a dark yellow oil which was used in the next step without further purification. ESI-MS: 178.1 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.26 (d, J=3.0 Hz, 1H), 7.92 (dd, J=4.5, 1.4 Hz, 1H), 7.26 (ddd, J=8.5, 3.0, 1.4 Hz, 1H), 7.17 (ddd, 1=8.5, 4.5, 0.7 Hz, 1H), 3.69-3.51 (m, 2H), 2.80-2.58 (m, 2H), 2.46-2.33 (m, 1H), 1.92-1.33 (m, 5H), 1.24-1.02 (m, 1H).

Preparation of 7-bromo-1-methyl-3-({[(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 7-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 8) (0.5 g, 1.0 eq.), (3S)-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 17)(0.33 g, 1.0 eq.), NaOAc (0.15 g, 1.0 eq.) in MeOH (4.0 mL) was stirred overnight at RT under argon atmosphere. Then, the reaction mixture was cooled to 0° C., NaBH₄ (0.073 g, 1.02 eq.) was added in portions and the mixture was left stirring overnight at RT. Subsequently, the mixture was partitioned between DCM and NaHCO₃ saturated aq. solution. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the crude product (0.63 g, 67% yield) as a yellow solid which was used in the next step without further purification. ESI-MS: 427.1 [M+H]⁺.

Preparation of 7-bromo-1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A suspension of 7-bromo-1-methyl-3-({[(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.47 g, 1.0 eq.) and 2-methylpyridine-4-carbaldehyde (0.13 g, 1.0 eq.) in DCE (8.0 mL) was stirred overnight at RT. Then, NaBH(OAc)₃ (0.33 g, 1.4 eq.) was added and the resulting mixture was left stirring overnight at RT. Subsequently, the mixture was partitioned between DCM and sat. NaHCO₃ aq. solution. The organic layer was washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.38 g, 62% yield) as a yellow solid. ESI-MS: 532.1 [M+H]⁺.

Preparation of methyl 1-[1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylate

A pressure vessel was charged with 7-bromo-1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.1 g, 1.0 eq.), methyl piperidine-4-carboxylate (0.05 mL, 2.0 eq.), Cs₂CO₃ (0.122 g, 2.0 eq.), Xantphos (0.022 g, 0.2 eq.) and Pd₂(dba)₃ (0.017 g, 0.1 eq.). Then, the vessel was capped, the evacuated of air and filled with argon. Subsequently, 1,4-dioxane (1.0 mL) was added via a syringe and the resulting mixture was left stirring overnight at 80° C. Then, the reaction mixture was cooled to RT, filtered through a pad of celite and the pad was washed with DCM and EtOAc. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep-H PLC (H₂O:MeCN:NH₃) to give the product (0.026 g, 23% yield) as a white powder. ESI-MS: 595.5 [M+H]⁺.

Preparation of 1-[1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylic Acid

LiOH.H₂O (0.004 g, 3.0 eq.) was added to a solution of 1-[1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylate (0.02 g, 1.0 eq.) in a mixture of THF (1.0 mL) and H₂O (1.0 mL) and the resulting mixture was stirred for 2 h at RT. Then, the reaction mixture was concentrated in vacuo and the residue was diluted with water, acidified using 1 M HCl aq. solution and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The isolated sample was freeze-dried (×2) to give the product (0.014 g, 71% yield) as a yellow solid. ESI-MS: 581.4 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 14. (brs, 1H), 8.38-8.19 (m, 2H), 7.96 (d, J=9.1 Hz, 1H), 7.91 (dd, J=4.5, 1.3 Hz, 1H), 7.85 (s, 1H), 7.32-7.19 (m, 3H), 7.14 (dd, J=8.5, 4.5 Hz, 1H), 7.06 (dd, J=9.1, 2.1 Hz, 1H), 6.67 (d, J=2.1 Hz, 1H), 4.01-3.83 (m, 3H), 3.81-3.48 (m, 9H), 3.01-2.90 (m, 2H), 2.88-2.76 (m, 1H), 2.71-2.56 (m, 2H), 2.39 (s, 3H), 2.04-1.84 (m, 3H), 1.81-1.35 (m, 5H).

Example 47. 1-Cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

NaH (0.02 g, 1.5 eq.) was added in two portions to a mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.2 g, 1.0 eq.) and ethylene glycol (3.0 mL). The resulting mixture was heated for 4 h at 120° C. Then, MeOH (2.0 mL) was added followed by H₂O (5.0 mL) and the mixture was washed with DCM (×4). The combined organic layers were washed with water, brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.032 g, 16% yield) as a beige solid. ESI-MS: 572.6 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.30-8.24 (m, 1H), 8.15-8.10 (m, 1H), 7.84 (s, 1H), 7.79 (d, J=11.7 Hz, 1H), 7.51 (d, J=7.3 Hz, 1H), 7.25-7.18 (m, 2H), 7.18-7.13 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 5.12-4.93 (m, 1H), 4.30-4.18 (m, 2H), 3.88-3.69 (m, 5H), 3.65-3.55 (m, 3H), 3.55-3.47 (m, 1H), 2.81-2.69 (m, 2H), 2.61-2.55 (m, 1H), 2.37 (s, 3H), 2.32 (s, 3H), 2.01-1.88 (m, 1H), 1.83-1.71 (m, 1H), 1.64-1.39 (m, 2H), 1.30-1.17 (m, 2H), 0.97-0.77 (m, 2H).

Example 48. 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(5-methyl-1,2,4-oxadiazol-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(5-methyl-1,24-oxadiazol-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A suspension of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 28) (0.107 g, 1.0 eq.), 3-bromo-5-methyl-1,2,4-oxadiazole (0.043 g, 1.3 eq.) and Cs₂CO₃ (0.10 g, 1.5 eq.) in anh. 1,4-dioxane (3.0 mL) was purged with argon for 10 min. Subsequently, Xantphos (0.014 g, 0.12 eq.) and Pd₂(dba)₃ (0.011 g, 0.06 eq.) were added. After stirring at 115° C. for 15 h, the mixture was filtered through celite, concentrated in vacuo and purified by FCC (SiHP; DCM:MeOH). The obtained product was dissolved in DCM and scavenger QuadraPure MPA (0.2 g) was added. After stirring for 1 h, the scavenger was filtrated off and filtrate was concentrated under reduced pressure and re-purified by FCC (C18HP; H₂O:MeCN). The product was converted to the HCl salt using 2 M HCl in Et₂O (0.028 mL, 1.0 eq.) and DCM as a solvent (2.0 mL) to provide the product (0.035 g, 28% yield) as an orange solid. ESI-MS: 588.7 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.68 (d, J=6.2 Hz, 1H), 8.37 (s, 1H), 8.13 (s, 1H), 8.10-7.93 (m, 1H), 7.79 (d, J=14.4 Hz, 1H), 7.08 (d, J=7.6 Hz, 1H), 4.74-4.67 (m, 1H), 4.62-4.54 (m, 1H), 4.49 (s, 2H), 4.36-4.10 (m, 1H), 3.97-3.59 (m, 7H), 3.56-3.44 (m, 2H), 3.22-2.97 (m, 1H), 2.76 (s, 3H), 2.45 (s, 3H), 2.39-2.28 (m, 1H), 2.27-2.08 (m, 2H), 2.03-1.91 (m, 1H), 1.80-1.55 (m, 2H), 1.50-1.39 (m, 2H), 1.24-1.08 (m, 2H).

Example 49. 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of ethyl (2Z)-2-[(Z)-4-bromo-2-fluorobenzoyl]-3-(dimethylamino)prop-2-enoate

The reaction was divided into two identical batches and the same procedure was used for each reactor. A mixture of ethyl 3-(dimethylamino)prop-2-enoate (7.58 mL, 1.0 eq.) and anh. N,N-Diisopropylethylamine, (19.3 mL, 2.1 eq.) was stirred for 10 minutes, after which time a solution of 4-bromo-2-fluorobenzoyl chloride (12.5 g, 1.0 eq.) in anh. toluene (100.0 mL) was added dropwise to the reaction mixture and then heated at 90° C. for 4 h. A colorless precipitate formed. The precipitate was filtered off on a sintered funnel and the solvents of the filtrate were evaporated under reduced pressure. The resulting oily product was then dry-deposited on silica gel and purified via FCC (SiHP; Hex:EtOAc) to give desired product (26.5 g, 64% yield) as a dark orange oil. ESI-MS: 345.9 [M+2+H]⁺.

Preparation of ethyl 7-bromo-4-oxo-7-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylate

A mixture of ethyl (2Z)-2-[(Z)-4-bromo-2-fluorobenzoyl]-3-(dimethylamino)prop-2-enoate (26.5 g, 1.0 eq.) and propan-2-amine (6.0 mL, 1.1 eq.) in anh. toluene (200.0 mL) was heated at 110° C. for 5 hours. Solvent was evaporated and the resulting crude material was dissolved in DME (200.0 mL) and cooled to 0° C., after which sodium hydride (60% dispersion in mineral oil, 5.4 g, 2.0 eq.) was added slowly. The resulting reaction mixture was then heated to 80° C. for another 6 h after which it was cooled to RT. The mixture was diluted with EtOAc, saturated ammonium chloride solution was added and the mixture was washed with additional portions of ethyl acetate. The organic phase was washed with brine, dried over Na₂SO₄ and the solvent removed under reduced pressure. Some product remained in the form of solid residue and some dissolved in the aqueous layer. The solid residues were filtered off and the desired product in the aqueous layer was extracted into DCM. The fraction contained in the ethyl acetate was purified via FCC (SiHP; Hex:EtOAc). was All fractions were combined to yield the product (13.7 g, 56% yield) to be used in the next step without further purification. ESI-MS: 338.9 [M+2]⁺.

Preparation of 7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylic Acid

Saturated hydrochloric acid (20.0 mL) was added to a cooled in ice-water bath suspension of ethyl 7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylate (13.7 g, 1.0 eq.) in 1,4-dioxane (60.0 mL) and water (50.0 mL) was added and the resulting mixture was then heated at 90° C. for 2 days. The reaction mixture was cooled to RT and the resulting precipitate filtered off using a sintered funnel. It was washed with additional portions of water and dried. The resulting solid with residual water was dissolved in DCM. This solution was washed with brine, dried over Na₂SO₄ and the solvent removed under reduced pressure to give the product (12.4 g, 98% yield) that was then used in the next step without further purification. ESI-MS: 311.1 [M+H]⁺.

Preparation of 7-bromo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinolin-4-one

Sodium borohydride (6.2 g, 4.5 eq.) was added to a cooled in ice-water bath solution of 7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carboxylic acid (12.4 g, 1.0 eq.) in methanol (400.0 mL), kept under argon, slowly over 5 min. The mixture was warmed to RT and was stirred for 4 h. p-Toluene sulfonic acid (0.69 g, 0.1 eq.) was then added and the reaction mixture was heated at 60° C. for 6 h. Silica gel (40 g) was added to the reaction mixture and the solvent removed under reduced pressure. The crude product, now adsorbed on silica, was purified by FCC (SiHP; Hex:EtOAc) to give product (8.03 g, 81% yield). ESI-MS: 269.0 [M+H]⁺.

Preparation of 7-bromo-4-oxo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinoline-3-carbaldehyde

7-bromo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinolin-4-one (8.0 g, 1.0 eq.) dissolved in THF (20 mL) was added at 0° C. to a solution of sodium bis(trimethylsilyl)amide solution (17.7 mL, 1.2 eq.) in THF (50 mL). The reaction mixture was stirred for 30 min at 0° C. and then, ethyl formate (2.9 mL, 1.2 eq.) dissolved in THF (10 mL) was added. After 1 h the reaction was quenched by addition of saturated NH₄Cl, which was followed by washing the product mixture with ethyl acetate (×3). The combined organic layers were washed with brine, dried over Na₂SO₄, and the solvent removed under reduced pressure. The resulting product (9.02 g, 69% yield) was used directly in next step without further purification. ESI-MS: 296.0, 297.9 [M+H]⁺.

Preparation of 7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 19)

7-Bromo-4-oxo-1-(propan-2-yl)-1,2,3,4-tetrahydroquinoline-3-carbaldehyde (9.02 g, 1.0 eq.) was dissolved in anh. 1,4-dioxane (200.0 mL). MnO₂ (8.87 g, 5.0 eq.) was added and the reaction was heated at 50° C. for 2 days. The reaction mixture was cooled to RT and filtered through celite. The pad of celite was washed with the mixture of DCM:MeOH (7:3). The filtrate was concentrated under reduced pressure and the residue was redissolved in 200.0 mL of DCM. The yellow solids that precipitated were filtered on a sintered funnel and washed with additional portions of DCM to give product (3.95 g, 64% yield). The crude product mixture was purified by FCC (SiHP; DCM:MeOH) to give the product (0.375 g, 6% yield). ESI-MS: 295.9 [M+2+H]⁺.

Preparation of tert-butyl N-[(3S)-1-(pyridazin-4-yl)piperidin-3-yl]carbamate

In a sealed tube, tert-Butyl N-[(3S)-piperidin-3-yl]carbamate (0.22 g, 1.3 eq.), 4-bromopyridazine hydrobromide (0.2 g, 1.0 eq.) and cesium carbonate (0.543 g, 2.0 eq.) were suspended in dry 1,4-dioxane. The reaction mixture was purged with argon for 10 min and Xantphos (0.029 g, 0.06 eq.) and Pd₂(dba)₃ (0.038 g, 0.05 eq.) were added. The reaction mixture was purged one more time with argon for 5 min, the tube was closed and reaction was heated at 100° C. overnight. Dichloromethane and water were added to the mixture and the compound was partitioned between the two layers. The organic layer was dried over anh. MgSO₄, filtered and concentrated. The crude mixture was purified by FCC (SiHP, DCM:MeOH) to give the product (0.125 g, 49% yield). ESI-MS: 279.4 [M+H]⁺.

Preparation of (3S)-1-(pyridazin-4-yl)piperidin-3-amine 4.0 M HCl in 1,4-dioxane (0.66 mL, 5.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-(pyridazin-4-yl)piperidin-3-yl]carbamate (0.152 g, 1.0 eq.) in 1,4-dioxane. The reaction mixture was stirred for 1 h at 60° C. The crude mixture was concentrated and partitioned between with DCM and NaOH aq. The organic layers were combined, dried over Na₂SO₄, filtered and concentrated under reduced pressure. The aqueous phase was freeze-dried since some product was detected in it and the combined residues were purified by FCC (PF-NH₂; DCM:MeOH) to give the product (0.065 g, 70% yield). ESI-MS: 179.0 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridazin-4-yl)piperidin-3-amine

(3S)-1-(Pyridazin-4-yl)piperidin-3-amine (0.065 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.039 mL, 1.0 eq.) and sodium acetate (0.029 g, 1.0 eq.) were dissolved in methanol (3.0 mL) and the reaction mixture was stirred at RT overnight. Then the mixture was cooled to 0° C. and sodium borohydride (0.015 g, 1.1 eq.) was added in portions and the reaction mixture was stirred at RT for 1 h. Methanol was evaporated and The product was purified by FCC (SiHP, DCM:MeOH) to give the product (0.079 g, 73% yield) as a yellowish oil. ESI-MS: 284.1 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.84 (dd, J=3.4, 0.8 Hz, 1H), 8.53 (dd, J=6.6, 0.8 Hz, 1H), 8.33 (d, J=5.5 Hz, 1H), 7.32 (d, J=1.5 Hz, 1H), 7.26 (d, J=5.4 Hz, 1H), 6.98 (dd, J=6.7, 3.4 Hz, 1H), 4.02 (dd, J=13.3, 3.6 Hz, 1H), 3.90 (s, 1H), 3.85 (s, 1H), 3.23-3.12 (m, 1H), 3.04 (dd, J=13.2, 9.1 Hz, 1H), 2.72 (td, J=9.1, 4.3 Hz, 1H), 2.52 (s, 3H), 2.15-2.04 (m, 1H), 1.97 (s, 2H), 1.92-1.85 (m, 1H), 1.64-1.49 (m, 2H).

Preparation of 7-bromo-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 19) (0.078 g, 1.0 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridazin-4-yl)piperidin-3-amine (0.079 g, 1.0 eq.) were dissolved in DCE. The resulting mixture was stirred at RT overnight. Then sodium triacetoxyborohydride (0.15 g, 2.8 eq.) was added and mixture was stirred for another 24 h at RT. The reaction mixture was diluted with DCM, washed with sat. aq. sodium bicarbonate, water, brine, dried over anh. MgSO₄ and concentrated under reduced pressure. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.066 g, 45% yield) as an off-white foam. ESI-MS: 561.3, 563.1 [M+H]⁺.

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

A solution of 7-bromo-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.066 g, 1.0 eq.), (3R)-pyrrolidin-3-ol (0.015 g, 1.5 eq.) and cesium carbonate (0.067 g, 1.8 eq.) in anh. DMF (1.5 mL) taken in a sealed tube was purged with argon for 15 min. Then BINAP (0.014 g, 0.2 eq.) and Pd₂(dba)₃ (0.011 g, 0.1 eq.) were added. The reaction mixture was purged with argon for another 2 min and the tube was sealed. The resulting mixture was heated to 110° C. for 20 h and the mixture was filtered through a pad of celite. The filtrate was sonicated with scavenger, filtered on a pad of cotton and concentrated. The crude mixture was purified by FCC (SiHP; DCM:MeOH) to give the product (0.053 g, 80% yield) as a beige solid. ESI-MS: 568.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.95 (d, J=3.2 Hz, 1H), 8.54 (d, J=6.4 Hz, 1H), 8.30 (d, J=5.0 Hz, 1H), 7.99 (d, J=8.9 Hz, 1H), 7.80 (s, 1H), 7.29 (s, 1H), 7.21 (d, J=5.2 Hz, 1H), 6.93 (dd, J=6.5, 3.3 Hz, 1H), 6.65 (dd, J=9.0, 1.9 Hz, 1H), 6.41 (d, J=2.0 Hz, 1H), 5.01 (d, J=3.7 Hz, 1H), 4.96-4.89 (m, 1H), 4.46-4.38 (m, 1H) J, 4.18 (d, J=12.9 Hz, 1H), 3.98 (d, J=13.1 Hz, 1H), 3.78 (s, 2H), 3.63 (s, 2H), 3.51 (dd, J=10.6, 4.8 Hz, 1H), 3.48-3.39 (m, 2H), 3.21 (d, J=10.2 Hz, 1H), 3.06 (t, J=11.8 Hz, 1H), 2.84 (t, J=12.6 Hz, 1H), 2.62 (s, 1H), 2.40 (s, 3H), 2.10-2.01 (m, 1H), 1.95 (s, 2H), 1.77 (d, J=13.7 Hz, 1H), 1.67 (dd, J=14.0, 10.4 Hz, 1H), 1.38 (t, J=7.0 Hz, 6H).

Example 50 and Example 51. Diastereomers A and B of 7-(4-amino-3,3-difluoropiperidin-1-yl)-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one: Example 50. 7-[(4R)-4-Amino-3,3-difluoropiperidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (A); Example 51. 7-[(4S)-4-Amino-3,3-difluoropiperidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (B)

Preparation of tert-butyl N-{3,3-difluoro-1-[1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidin-4-yl}carbamate

A solution of 7-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 13) (0.30 g, 1.0 eq.), tert-butyl N-(3,3-difluoropiperidin-4-yl)carbamate (0.26 g, 2.0 eq.) and cesium carbonate (0.372 g, 2.1 eq.) in anh. 1,4-dioxane taken in a sealed tube was purged with argon for 15 min. Then BINAP (0.102 g, 0.3 eq.) and Pd₂(dba)₃ (0.050 g, 0.1 eq.) were added. The reaction mixture was purged with argon for another 2 min and the tube was sealed. The resulting mixture was heated to 95° C. for 2 days and it was filtered on a pad of celite. Scavenger QuadraPure MPA was added to the filtrate and mixture was sonicated. Then the scavenger was filtered off and solvent was concentrated. The crude mixture was purified by FCC (SiHP, DCM:MeOH) to product (0.27 g, 67% yield). ESI-MS: 702.6 [M+H]⁺. Chiral separation of this racemate was performed (IF; Hex:EtOH 35% isocratic) to provide Boc-protected diastereomers A (0.085 g, 32% yield) and B (0.114 g, 43% yield). ESI-MS: 702.6 [M+H]⁺. Stereochemistry was assigned arbitrary.

Preparation of 7-[(4R)-4-Amino-3,3-difluoropiperidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one—Diastereomer A

4 M hydrogen chloride solution in 1,4-dioxane (0.143 mL, 5.0 eq.) was added to a solution of Boc-protected diastereomer A (0.085 g, 1.0 eq.) in 1,4-dioxane. The reaction mixture was stirred overnight at RT. However, the reaction did not go to completion and an additional portion of 4 M hydrogen chloride solution in 1,4-dioxane (0.143 mL, 5.0 eq.) was added and reaction time was prolonged to 2 days. Solvents was concentrated, extracted with DCM and NaOH aq. Organic layers were combined, dried over anh. MgSO₄, filtrated and concentrated. Crude was purified by FCC (PF-diol, DCM:MeOH) to give a product (0.020 g, 29% yield). ESI-MS: 602.5 [M+H]⁺.

Preparation of 7-[(4S)-4-Amino-3,3-difluoropiperidin-1-yl]-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one—Diastereomers B

4 M hydrogen chloride solution in 1,4-dioxane (0.2 mL, 5.0 eq.) was added to a solution of Boc-protected diastereomer B (0.114 g, 1.0 eq.) in 1,4-dioxane. The reaction mixture was stirred overnight at RT and the mixture was concentrated and partitioned between DCM and aq. NaOH. The organic layer was combined, dried over MgSO₄, filtered and concentrated under reduced pressure. The crude mixture was purified by FCC (PF-diol, DCM:MeOH) to give product (0.021 g, 22% yield). ESI-MS: 602.4 [M+H]⁺. 1H NMR (400 MHz, Methanol-d₄) δ 8.16 (s, 1H), 8.15 (d, J=3.4 Hz, 1H), 8.08 (d, J=2.9 Hz, 1H), 7.83 (s, 1H), 7.34 (dd, J=8.6, 3.0 Hz, 1H), 7.25-7.21 (m, 2H), 7.17 (dd, J=9.3, 2.2 Hz, 1H), 7.10 (d, J=8.6 Hz, 1H), 6.76 (d, J=2.2 Hz, 1H), 4.25-4.16 (m, 1H), 3.99 (d, J=13.7 Hz, 1H), 3.88 (d, J=10.9 Hz, 1H), 3.83 (d, J=4.0 Hz, 2H), 3.78 (s, 5H), 3.60 (d, J=12.2 Hz, 1H), 3.22-3.12 (m, 2H), 2.98-2.90 (m, 1H), 2.84 (t, J=11.1 Hz, 1H), 2.68 (t, J=11.1 Hz, 1H), 2.40 (s, 3H), 2.32 (s, 3H), 2.15 (s, 1H), 2.09-2.01 (m, 1H), 1.94-1.88 (m, 1H), 1.76 (q, J=11.7 Hz, 1H), 1.65 (q, J=8.8 Hz, 2H).

Example 52. 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of 7-bromo-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (Intermediate 20)

A solution of (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.57 g, 1.1 eq.) and 7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 19)(0.5 g, 1.0 eq.) in anh. DCE (10.0 mL) was stirred in 60° C. over 2 h. Then NaBH(OAc)₃ (0.98 g, 2.8 eq.) was added and the reaction mixture was stirred for an additional 2 h at 60° C. The mixture was diluted with DCM and washed with aq. solution of NaOH, dried over anh. Na₂SO₄, concentrated in vacuo and purified by FCC (SiHP; DCM:MeOH) to give product (0.64 g, 65% yield) as a yellow solid. ESI-MS: 576.1 [M+2+H]⁺.

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

In a sealed tube, (3R)-pyrrolidin-3-ol (0.066 g, 1.5 eq.), Cs₂CO₃ (0.294 g, 1.8 eq.) and 7-bromo-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (Intermediate 20) (0.3 g, 1.0 eq.) were suspended in anh. 1,4-dioxane (5.0 mL). The suspension was purged with argon for 5 min and then BINAP (0.062 g, 0.2 eq.) and Pd₂(dba)₃ (0.046 g, 0.1 eq.) were added. The reaction mixture was purged with argon one more time, the tube was closed and stirred at 120° C. overnight. The residue was filtered through celite, the solvent was concentrated in vacuo and purified by FCC (SiHP; DCM:MeOH) and repurified by RP FCC (C18HP; H₂O:MeCN) to afford (0.178 g, 61% yield) as a white solid. ESI-MS: 581.2 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) 8.30 (d, J=5.0 Hz, 1H), 8.12 (d, J=2.9 Hz, 1H), 7.99 (d, J=9.0 Hz, 1H), 7.82 (s, 1H), 7.27 (s, 1H), 7.24-7.17 (m, 2H), 7.00 (d, J=8.5 Hz, 1H), 6.65 (dd, J=8.9, 2.0 Hz, 1H), 6.41 (d, J=2.0 Hz, 1H), 5.01 (d, J=3.6 Hz, 1H), 4.98-4.89 (m, 1H), 4.43 (s, 1H), 3.86-3.70 (m, 3H), 3.63-3.55 (m, 3H), 3.54-3.36 (m, 3H), 3.26-3.16 (m, 1H), 2.79-2.69 (m, 2H), 2.52 (s, 1H), 2.40 (s, 3H), 2.32 (s, 3H), 2.12-2.00 (m, 1H), 1.99-1.88 (m, 2H), 1.76 (d, J=10.7 Hz, 1H), 1.60-1.44 (m, 2H), 1.43-1.32 (m, 6H).

Example 53. 7-[4-(2-Hydroxyethyl)piperazin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of 7-[4-(2-hydroxyethyl)piperazin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

7-Bromo-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (Intermediate 20) (0.043 g, 1.0 eq.), 2-(piperazin-1-yl)ethan-1-ol (0.019 g, 2.0 eq.) and cesium carbonate (0.043 g, 1.8 eq.) were dissolved in anh. 1,4-dioxane (2.0 mL). The solution was purged by argon for 5 min and then BINAP (0.009 g, 0.2 eq.) and Pd₂(dba)₃ (0.007 g, 0.1 eq.) were added. The tube was closed and stirred at 110° C. overnight. The reaction mixture was filtered through celite, water was added and the mixture was washed with DCM (×3). The combined organic layers were washed with brine, dried over MgSO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The obtained product was dissolved in methanol and stirred with scavenger QuadraPure MPA (0.2 g) for 1 h. The scavenger was filtered off and the filtrate was concentrated to give the product (0.039 g, 85% yield) as an off-white powder. ESI-MS: 624.5 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.30 (d, J=5.0 Hz, 1H), 8.12 (d, J=2.9 Hz, 1H), 8.01 (d, J=9.0 Hz, 1H), 7.88 (s, 1H), 7.30-7.16 (m, 3H), 7.10-6.95 (m, 2H), 6.89 (s, 1H), 5.01 (quint, J=6.6 Hz, 1H), 4.45 (t, J=5.3 Hz, 1H), 3.84-3.69 (m, 3H) J), 3.61 (s, 2H), 3.54 (q, J=5.8 Hz, 3H), 2.83-2.66 (m, 3H), 2.64-2.54 (m, 6H) J, 2.44 (t, J=6.2 Hz, 3H), 2.39 (s, 3H), 2.32 (s, 3H), 1.95 (s, 1H), 1.76 (d, J=10.3 Hz, 1H), 1.50 (q, J=11.9 Hz, 2H), 1.38 (dd, J=6.4, 2.6 Hz, 6H).

Example 54. 7-[(3R,4S)-3,4-dihydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of (3aR,6aR)-6-(hydroxymethyl)-2,2-dimethyl-tetrahydro-2H-furo[3,4-d][1,3]dioxol-4-ol

Iodine (0.01 g, 0.003 eq.) was added to a solution of D-ribose (2.0 g, 1.0 eq.) in acetone (HPLC Grade 99.5+%, 10.0 mL) and the resulting yellow solution was stirred at RT overnight. The reaction mixture was concentrated to dryness. The residue was dissolved in satd. aq. solution of sodium thiosulphate (50.0 mL), stirred for 10 min. and washed with EtOAc (200 mL×4). The combined organic layers were dried over MgSO₄, filtered and concentrated in vacuo to give product (2.46 g, 97% yield) as a colorless oil, that was used directly in the next step.

Preparation of (4R,5S)-2,2-dimethyl-7,3-dioxolane-4,5-dicarbaldehyde

A solution of (3aR,6aR)-6-(hydroxymethyl)-2,2-dimethyl-tetrahydro-2H-furo[3,4-d][1,3]dioxol-4-ol (1.58 g, 1.0 eq.) in water (15.0 mL) was added dropwise to a solution of NaIO₄ (3.55 g, 2.0 eq.) in water (LC-MS Grade, 80.0 mL) at 0° C. The reaction mixture was stirred at 0° C. for 30 min, warmed to RT and stirred at this temperature overnight. After the disappearance of the starting material as detected by TLC (petroleum ether/EtOAc=1:2), the reaction mixture was concentrated under reduced pressure. The residue was diluted with EtOAc (100.0 mL) and then filtered through a pad of celite. The filtrate was concentrated under vacuum to give product (1.50 g) as a colorless oil, which was used immediately in the next step.

Preparation of (3aR,6aS)-5-benzyl-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrole

A solution of (4R,5S)-2,2-dimethyl-1,3-dioxolane-4,5-dicarbaldehyde (1.3 g, 1.0 eq.) in anh. MeOH (10.0 mL) was added dropwise to a suspension of activated 3 Å molecular sieves, NaBH₃CN (1.12 g, 2.6 eq.) and zinc chloride (3.85 mL, 1.9 M in 2-MeTHFm 1.0 eq.) in 70.0 mL of anh. MeOH at 0° C. and was followed by the addition of benzylamine (ReagentPlus 99%, 1.0 mL, 1.2 eq.). The reaction mixture was stirred at 0° C. for 30 min, brought to RT and stirred at this temperature overnight. After the disappearance of the starting material as detected by TLC (petroleum ether/EtOAc=2:1), the reaction mixture was filtered through a pad of celite, and then the solvent was evaporated under reduced pressure. The residue was diluted with 100.0 mL of water, and then washed with EtOAc (50 mL×4). The combined organic layers were washed with brine, dried over MgSO₄ and concentrated under reduced pressure. The residue was purified by FCC (SiHP; Hex:EtOAc) to give a product (1.34 g, 75% yield) as a colorless oil. ESI-MS: 234.4 [M+H]⁺; ¹H NMR (300 MHz, CDCl₃) δ 7.37-7.19 (m, 5H), 4.70-4.60 (m, 2H), 3.63 (s, 2H), 3.04 (d, J=12.0 Hz, 2H), 2.28-2.05 (m, 2H), 1.57 (s, 3H), 1.32 (s, 3H).

Preparation of (3aR,6aS)-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrole

In a parr shaker vessel (3aR,6aS)-5-benzyl-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrole (1.18 g, 1.0 eq.) was dissolved in anh. MeOH (100.0 mL) and the solution was purged with argon for 10 min. Palladium hydroxide on carbon (0.48 g, 0.7 eq.) was added and argon was bubbled for another 20 min. The vessel was placed in the parr shaker apparatus under 40 bar H₂ and the reaction was carried out overnight. The solution was filtered through a pad of celite, washed with methanol and the filtrate was concentrated in vacuo to give product (0.652 g, 95% yield) as a colorless oil J J.

Preparation of 7-[(3aR,6aS)-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrol-5-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

A suspension of (3aR,6aS)-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrole (0.1 g, 2.3 eq.), Cs₂CO₃ (0.207 g, 2.1 eq.), 7-bromo-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (Intermediate 20) (0.174 g, 1.0 eq.) in anh. DMF (5.0 mL) was purged with argon for 10 min. BINAP (0.06 g, 0.3 eq.) and Pd₂(dba)₃ (0.06 g, 0.2 eq.) were then added. The reaction mixture was sonicated and purged with argon for 5 min. The mixture was then stirred at 115° C. overnight. The mixture was cooled and filtered through a pad of celite. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH), redissolved in DCM and stirred with scavenger QuadraPure MPA overnight. Subsequently, the mixture was filtered, concentrated in vacuo to give the product (0.17 g, 85% yield) as a brown semisolid. ESI-MS: 637.5 [M+H]⁺.

Preparation of 7-[(3R,4S)-3,4-dihydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

7-[(3aR,6aS)-2,2-dimethyl-hexahydro-[1,3]dioxolo[4,5-c]pyrrol-5-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.148 g, 1.0 eq.) was dissolved in a mixture of water (LC-MS Grade) and THF (2.0 mL, 1/1 v/v) then trifluoroacetic acid (0.05 mL, 3.0 eq.) was added. The resulting reaction mixture was stirred at RT overnight. The reaction mixture was basified to pH 12 using 2 M NaOH aq. solution and washed with DCM (4×50.0 mL). The combined organic layers were washed with water (10.0 mL) followed by brine (20.0 mL). The organic layer was dried over MgSO₄, filtered and concentrated in vacuo to give a brown residue. The crude material was purified by prep-H PLC (H₂O:MeCN:NH₃). The pure fractions were lyophilized and dried under high vacuum to give the product (0.033 g, 23% yield) as a yellow solid. ESI-MS: 597.6 [M+H]⁺; ¹H NMR (400 MHz, DMSO-d₆) δ 8.35 (s, 2H), 8.30 (d, J=5.1 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.98 (d, J=9.0 Hz, 1H), 7.82 (s, 1H), 7.27 (s, 1H), 7.23-7.16 (m, 2H), 7.00 (d, J=9.0 Hz, 1H), 6.62 (dd, J=9.0, 1.9 Hz, 1H), 6.38 (s, 1H), 4.94 (quint, J=6.5 Hz, 1H), 4.17 (t, J=4.3 Hz, 2H), 3.85-3.70 (m, 3H), 3.63-3.46 (m, 5H), 3.23 (dd, J=10.1, 4.1 Hz, 2H), 2.80-2.68 (m, 2H), 2.39 (s, 3H), 2.32 (s, 3H), 2.02-1.90 (m, 1H), 1.83-1.70 (m, 1H), 1.60-1.42 (m, 3H), 1.38 (dd, J=6.5, 4.2 Hz, 6H).

Example 55. 1-Cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]-N-methylcarbamate

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3)(1.0 g, 1.0 eq.), (R)-3-(N-Boc-N-methylamino)pyrrolidine (0.51 g, 1.5 eq.) and cesium carbonate (1.78 g, 3.2 eq.) in 1,4-dioxane (30 mL) was sparged with argon for 10 min, after which Pd₂(dba)₃ (0.31 g, 0.2 eq.) and Xantphos (0.30 g, 0.3 eq.) were added. The mixture was once again sparged with argon for 5 min, after which the reaction vessel was sealed and heated at 115° C. overnight. The mixture was passed through a pad of celite and washed with additional portions of EtOAc. The filtrate was then concentrated under reduced pressure and purified by FCC (SiHP deactivated with DCM:NH₃; DCM:MeOH) followed by RP-FCC (C18HP; H₂O:MeCN) yield the product (0.75 g, yield 61% yield) as a beige solid. ESI-MS: 710.7 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

tert-Butyl N-[(3R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]-N-methylcarbamate (0.728 g, 1.0 eq.) was dissolved in anh. DCM (20 mL) and trifluoroacetic acid (10 mL) was added slowly. The reaction mixture was stirred for 6 h at RT and then concentrated in vacuo. The residue was dissolved in DCM, water was added and the mixture was basified to pH 9 using 1 M aq. solution of NaOH and extracted by DCM. The organic layers were then washed with brine, dried over Na₂SO₄ and concentrated. The residue was purified by RP-FCC (C18HP; H₂O:MeCN), the resulting compound was dissolved in DCM and stirred for 4 h after the addition of the scavenger QuadraPure MPA (0.9 g). The scavenger was filtered off and filtrate was concentrated in vacuo. The resulting compound was converted into hydrochloride salt using 2 M HCl in Et₂O (0.34 mL, 1.0 eq.) and MeOH (14.0 mL) and water (4.0 mL) as solvents to provide the product ((0.420 g, 74% yield). ESI-MS: 610.6 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.17 (d, J=5.3 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 7.88 (s, 1H), 7.82 (d, J=14.5 Hz, 1H), 7.56 (dd, J=8.7, 3.0 Hz, 1H), 7.34-7.22 (m, 3H), 7.07 (d, J=7.5 Hz, 1H), 4.06-3.88 (m, 6H), 3.88-3.76 (m, 3H), 3.72-3.56 (m, 2H), 3.51-3.37 (m, 1H), 3.09-2.87 (m, 2H), 2.84 (s, 3H), 2.81-2.70 (m, 1H), 2.64-2.51 (m, 1H), 2.48 (s, 3H), 2.36 (s, 3H), 2.34-2.21 (m, 1H), 2.21-2.12 (m, 1H), 2.00-1.88 (m, 1H), 1.80-1.61 (m, 2H), 1.39-1.25 (m, 2H), 0.93 (p, J=4.3 Hz, 2H).

Example 56. and Example 57. Diastereomers A and B of 7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Example 56. (7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one hydrochloride (A) and Example 57. 7-[(1S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one) Hydrochloride (B)

Preparation of Boc-protected diastereomers tert-butyl N-[(1R)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-3,3-difluoropiperidin-4-yl]carbamate (A) and tert-butyl N-[(4S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-3,3-difluoropiperidin-4-yl]carbamate) (B)

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3)(1.85 g, 1.0 eq.), tert-butyl N-(3,3-difluoropiperidin-4-yl)carbamate (1.0 g, 1.3 eq.), Cs₂CO₃ (2.2 g, 2.1 eq.) in anh. 1,4-dioxane (70.0 mL) was purged with argon for 30 min. Then, BINAP (0.61 g, 0.3 eq.) and Pd₂(dba)₃ (0.30 g, 0.1 eq.) were added and the resulting mixture was purged with argon for another 15 min. The tube was sealed and the reaction mixture was heated for 2 days at 95° C. Subsequently, the mixture was filtered through a pad of celite and the pad was washed with MeOH. The filtrate was concentrated in vacuo and the residue was partitioned between DCM and water. The organic layer was washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH). The isolated sample was dissolved in DCM, scavenger QuadraPure MPA (1.3 g) was added and the resulting suspension was left stirring overnight at RT. Then, the scavenger was filtered off and the filtrate was concentrated in vacuo to give the mixture of Boc-protected diastereomers A and B (2.24 g, 86% yield) as a yellow solid. ESI-MS: 746.8 [M+H]⁺. The mixture was separated by chiral HPLC (ADH; Hex:EtOH 15% isocrat) to give Boc-protected diastereomer A (0.685 g, t_(R) 43.6 min) ESI-MS: 746.8 [M+H]⁺ and Boc-protected diastereomer B (0.656 g, t_(R) 61.2 min). ESI-MS: 746.8 [M+H]⁺, both as a yellow foams. The actual configurations of the diastereomers were not determined.

Preparation of (7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride—Diastereomer A

TFA (3.0 mL, 92.0 eq.) was added to a solution of Boc-protected Diastereomer A (0.325 g, 1.0 eq.) in DCM (6.5 mL) kept at 0° C. The resulting mixture was warmed to RT and stirred for 1 h. Then, the mixture was concentrated in vacuo, the residue was triturated with DCM and concentrated in vacuo (3 cycles). The obtained solid was partitioned between DCM and water after the aqueous layer was basified using 1 M NaOH aq. solution. The organic layer was dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The isolated sample was converted into the HCl salt using 2 M HCl solution in 1,4-dioxane (0.152 mL, 1.0 eq. to FB) and DCM (8.0 mL) as a solvent to give the product (0.205 g, 70% yield) as a yellow solid. ESI-MS: 646.6 [M+H]⁺. 1H NMR (400 MHz, Methanol-d₄) δ 8.15 (d, J=5.2 Hz, 1H), 8.10 (d, J=2.9 Hz, 1H), 7.91 (s, 1H), 7.87 (d, J=13.3 Hz, 1H), 7.49 (dd, J=8.7, 3.0 Hz, 1H), 7.45 (d, J=7.4 Hz, 1H), 7.28-7.18 (m, 3H), 4.14-4.01 (m, 1H), 3.98-3.58 (m, 8H), 3.48-3.33 (m, 2H), 3.22-3.12 (m, 1H), 3.06-2.85 (m, 2H), 2.82-2.67 (m, 1H), 2.44 (s, 3H), 2.33 (s, 3H), 2.29-2.17 (m, 1H), 2.19-2.10 (m, 1H), 2.11-1.98 (m, 1H), 1.98-1.82 (m, 1H), 1.76-1.57 (m, 2H), 1.39-1.23 (m, 2H), 1.00-0.85 (m, 2H).

Preparation of 7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one) Hydrochloride—Diastereomer B

TFA (3.0 mL, 93.0 eq.) was added to a solution of Boc-protected Diastereomer B (0.338 g, 1.0 eq.) in DCM (6.5 mL) kept at 0° C. The resulting mixture was stirred for 1 h at RT. Then, the mixture was concentrated in vacuo, the residue was triturated with DCM and concentrated in vacuo (3 cycles). The obtained solid was partitioned between DCM and water after the aqueous layer was basified using 1 M NaOH aq. solution. The combined organic layers were dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The isolated sample was converted into the HCl salt using 2 M HCl solution in 1,4-dioxane (0.153 mL, 1.0 eq. to FB) and DCM (8.0 mL) as a solvent to give the product (0.208 g, 71% yield) as a yellow solid. ESI-MS: 646.7 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.15 (d, J=5.2 Hz, 1H), 8.10 (d, J=2.9 Hz, 1H), 7.90 (s, 1H), 7.86 (d, J=13.4 Hz, 1H), 7.49-7.40 (m, 2H), 7.28-7.14 (m, 3H), 4.10-3.97 (m, 1H), 3.95-3.74 (m, 6H), 3.69-3.55 (m, 2H), 3.50-3.35 (m, 2H), 3.24-3.08 (m, 1H), 3.03-2.81 (m, 2H), 2.80-2.67 (m, 1H), 2.43 (s, 3H), 2.32 (s, 3H), 2.25-2.09 (m, 2H), 2.08-1.86 (m, 2H), 1.79-1.58 (m, 2H), 1.41-1.20 (m, 2H), 1.05-0.82 (m, 2H).

Example 58. 1-Cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]-N-methylcarbamate

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3)(1.0 g, 1.0 eq.), (S)-3-(N-Boc-N-methylamino)pyrrolidine (0.51 g, 1.5 eq.) and Cs₂CO₃ (1.78 g, 3.2 eq.) in anh. 1,4-dioxane (20.0 mL) was sparged with argon for 10 minutes, after which Pd₂(dba)₃ (0.31 g, 0.2 eq.) and Xantphos (0.30 g, 0.3 eq.) were added. The mixture was once again sparged with argon for 5 min, after which the reaction vessel was sealed and heated at 115° C. overnight. The mixture was passed through a pad of celite and washed with additional portions of EtOAc. The filtrate was then concentrated on a rotary evaporator. The crude material was purified by FCC (SiHP, DCM:MeOH) and repurified by RP-FCC (C18HP, H₂O:MeCN) to give product (0.799 g, 64% yield) as a beige solid. ESI-MS: 710.9 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

tert-Butyl N-[(3S)-1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]pyrrolidin-3-yl]-N-methylcarbamate (0.799 g, 1.0 eq.) was dissolved in anh. DCM (5.0 mL) and placed in an ice bath. Then, TFA (2.5 mL, 30.0 eq.) was added in slowly, stirring continued for 5 min and ice bath was removed. The reaction mixture was stirred for 3 h at RT, then concentrated under reduced pressure and diluted with water. This aqueous solution was basified with 2 M NaOH to pH value 10 and washed with DCM. The organic layer was dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by RP-FCC (C18HP, H₂O:MeCN). The product was redissolved in DCM (10.0 mL) and stirred for 4 h with the scavenger QuadraPure MPA (0.9 g). The mixture was filtered through a Buchner funnel and washed with DCM (×2). The filtrate was concentrated and repurified using FCC (PF-50DIOL, DCM:MeOH). The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.22 mL, 1.1 eq.) and DCM as a solvent (6.0 mL) to give product (0.272 g, yield 39% yield over 2 steps) as a yellow solid. ESI-MS: 610.6 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.20-8.12 (m, 2H), 7.86 (s, 1H), 7.82 (d, J=14.5 Hz, 1H), 7.68 (dd, J=9.0, 3.0 Hz, 1H), 7.39-7.31 (m, 2H), 7.29 (d, J=5.6 Hz, 1H), 7.06 (d, J=7.6 Hz, 1H), 4.07-3.93 (m, 4H), 3.92-3.76 (m, 5H), 3.74-3.53 (m, 2H), 3.47-3.38 (m, 1H), 3.10-2.93 (m, 2H), 2.88-2.74 (m, 4H), 2.61-2.52 (m, 1H), 2.50 (s, 3H), 2.35 (s, 3H), 2.34-2.21 (m, 1H), 2.21-2.12 (m, 1H), 2.01-1.88 (m, 1H), 1.84-1.60 (m, 2H), 1.35-1.25 (m, 2H), 0.98-0.86 (m, 2H).

Example 59. 7-[(3R)-3-Hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]carbamate

In a sealed tube, 5-bromopyrimidine (0.2 g, 1.0 eq.), tert-butyl N-[(3S)-piperidin-3-yl]carbamate (0.33 g, 1.3 eq.) and cesium carbonate (0.82 g, 2.0 eq.) were suspended in anh. 1,4-dioxane (5.0 mL). The reaction mixture was purged with argon for 10 min, then Xantphos (0.044 g, 0.06 eq.) and Pd₂(dba)₃ (0.058 g, 0.05 eq.) were added. The reaction mixture was purged one more time with argon for 5 min, then reaction tube was closed and reaction was heated at 100° C. overnight. The reaction mixture was diluted with water and extracted with DCM (×3). The combined organic layers were washed with brine, dried over MgSO₄, filtered and concentrated. The crude material was loaded on a column and purified by FCC (SiHP; DCM:MeOH) to give the product (0.301 g, 82% yield) as a yellow solid. ESI-MS: 279.1 [M+H]⁺.

Preparation of (3S)-1-(pyrimidin-5-yl)piperidin-3-amine

tert-Butyl N-[(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]carbamate (0.30 g, 1.0 eq.) was dissolved in anh. DCM (5.0 mL) and trifluoroacetic acid (0.79 mL, 10.0 eq.) was added and the reaction mixture was stirred at RT overnight. The solvent was evaporated and the crude product was further diluted with MeOH and traces of moisture were azeotropically removed (×3) to give product as a trifluoroacetate salt (0.3 g, 97% yield) which is a yellow oil. ESI-MS: 179.1 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyrimidin-5-yl)piperidin-3-amine

(3S)-1-(Pyrimidin-5-yl)piperidin-3-amine trifluoroacetate salt (0.30 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.11 mL, 1.0 eq.) and sodium acetate (0.083 g, 1.0 eq.) were dissolved in anh. methanol (8.0 mL) and the reaction mixture was stirred at RT overnight. Then the mixture was cooled to 0° C., sodium borohydride (0.042 g, 1.1 eq.) was added and the reaction mixture was stirred at RT overnight. Methanol was evaporated and the residue was dissolved in DCM, water was added and pH was adjusted to 11 by adding 2M aq. NaOH. The product mixture was washed with DCM (×3). The combined organic layers were washed with brine, dried over MgSO₄, filtered and concentrated in vacuo. The crude mixture was purified by FCC (SiHP; DCM:MeOH) to give the product (0.160 g, 54% yield) as a yellow oil. ESI-MS: 284.2 [M+H]⁺.

Preparation of 7-bromo-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

7-Bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 19) (0.166 g, 0.55 mmol, 1.0 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyrimidin-5-yl)piperidin-3-amine (0.160 g, 1.0 eq.) were dissolved in anh. DCE (3.0 mL) and the mixture was stirred at RT overnight. Then the solution was cooled to 0° C., NaBH(OAc)₃ (0.348 g, 3.0 eq.) was added and the reaction mixture was stirred at RT overnight. Water was added, pH was adjusted to 11 by addition of 1 M aq. solution of NaOH and the product was extracted by DCM (×3). The combined organic layers were dried over MgSO₄, filtered and concentrated in vacuo. Crude was purified by FCC (SiHP; DCM:MeOH) to give the product (0.187 g, 60% yield) as a white solid. ESI-MS: 561.3 [M+H], 563.2 [M+2+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.52 (s, 1H), 8.49 (s, 2H), 8.28 (d, J=5.1 Hz, 1H), 8.14-8.10 (m, 2H), 8.02 (s, 1H), 7.50 (dd, J=8.6, 1.6 Hz, 1H), 7.27 (s, 1H), 7.23-7.17 (m, 1H), 5.05 (hept, J=6.2 Hz, 1H), 4.06-3.98 (m, 1H), 3.83-3.72 (m, 3H), 3.72-3.63 (m, 2H), 2.91 (t, J=11.6 Hz, 1H), 2.76-2.66 (m, 2H), 2.38 (s, 3H), 2.00 (d, J=11.8 Hz, 1H), 1.79 (d, J=13.3 Hz, 1H), 1.66-1.45 (m, 2H), 1.39 (t, J=6.8 Hz, 6H).

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

7-Bromo-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrimidin-5-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.12 g, 1.0 eq.), (3R)-pyrrolidin-3-ol (0.028 g, 1.5 eq.) and cesium carbonate (0.13 g, 1.8 eq.) were dissolved in anh. 1,4-dioxane (4.0 mL) and the resulting suspension was purged with argon for 10 min, then BINAP (0.027 g, 0.2 eq.) and Pd₂(dba)₃ (0.02 g, 0.1 eq.) were added. The vessel was closed and stirred at 100° C. overnight. The reaction mixture was filtered through a pad of celite, water was added and the mixture was extracted with DCM three times. The combined organic layers were washed with brine, dried over MgSO₄, filtered and concentrated in vacuo. Purification by FCC (SiHP; DCM:MeOH) afforded the product (0.078 g, 63% yield) as a yellow solid. ESI-MS: 568.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.51 (s, 1H), 8.48 (s, 2H), 8.30 (d, J=5.0 Hz, 1H), 7.99 (d, J=8.9 Hz, 1H), 7.83 (s, 1H), 7.30 (s, 1H), 7.22 (d, J=4.8 Hz, 1H), 6.66 (dd, J=9.0, 1.9 Hz, 1H), 6.42 (d, J=2.1 Hz, 1H), 5.02 (d, J=3.7 Hz, 1H), 4.94 (hept, J=5.5 Hz, 1H), 4.47-4.40 (m, 1H), 4.01 (d, J=11.3 Hz, 1H), 3.84-3.71 (m, 3H), 3.67-3.57 (m, 2H), 3.51 (dd, J=10.6, 4.8 Hz, 1H), 3.47-3.39 (m, 2H), 3.22 (d, J=10.9 Hz, 1H), 2.89 (t, J=11.6 Hz, 1H), 2.77-2.64 (m, 2H), 2.41 (s, 3H), 2.13-2.00 (m, 1H), 2.02-1.89 (m, 2H), 1.83-1.75 (m, 1H), 1.65-1.43 (m, 2H), 1.39 (t, J=7.0 Hz, 6H).

Example 60. 1-[1-Cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-1,2,3-triazole-4-carboxamide

Preparation of 7-azido-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 21)

A suspension of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.700 g, 1.0 eq.) and sodium azide (0.27 g, 1.5 eq.) in anh. DMF (14.0 mL) was heated at 70° C. for 4 h. The reaction mixture was allowed to cool to RT and then poured into cold water (150.0 mL). The mixture was washed with EtOAc. The organic layer was washed with aqueous saturated solution of NaHCO₃ and brine, dried over anh. Na₂SO₄, filtered and concentrated. The product was used directly in the next step without further purification (0.820 g, 105% yield). ESI-MS: 273.0 [M+H]⁺.

Preparation of 7-azido-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 21)

A suspension of 7-azido-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.82 g, 1.0 eq.), (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.90 g, 1.05 eq.) and anh. sodium sulfate (0.41 g, 1.0 eq.) in anh. DCE (14.0 mL) was stirred in RT overnight, then sodium triacetoxyborohydride (1.7 g, 2.8 eq.) was slowly added in portions and the resulting reaction mixture was stirred overnight at RT. The reaction mixture was then washed using DCM and 1 M aqueous solution of NaOH. The organic layer was combined, washed with brine, dried over Na₂SO₄, filtered and concentrated. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.88 g, 54% yield) as a beige solid. ESI-MS: 553.6 [M+H]⁺.

Preparation of ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-1,2,3-triazole-4-carboxylate

Ethyl prop-2-ynoate (0.029 mL, 1.1 eq.), sodium ascorbate (0.006 g, 0.1 eq.) and copper(II) sulfate pentahydrate (0.003 g, 0.05 eq.) were sequentially added to a solution of 7-azido-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 21) (0.150 g, 1.0 eq.) in methanol (6.0 mL) and water (2.0 mL) and the reaction mixture was stirred at 40° C. for 2 h. Methanol was evaporated and the residue was diluted with DCM and water. The organic phase was separated and aqueous layer was washed with DCM. The combined organic layers were dried over MgSO₄, filtered and concentrated. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.143 g, 79% yield) as a yellow solid. ESI-MS: 651.7 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 9.39 (d, J=1.7 Hz, 1H), 8.47 (d, J=6.1 Hz, 1H), 8.29 (br s, 1H), 8.18-8.10 (m, 2H), 8.01 (s, 1H), 7.27-7.20 (m, 2H), 7.18 (d, J=5.0 Hz, 1H), 7.04 (d, J=8.4 Hz, 1H), 4.40 (q, J=7.1 Hz, 2H), 3.85-3.76 (m, 3H), 3.68 (s, 2H), 3.65-3.55 (m, 2H), 2.84-2.74 (m, 2H), 2.61 (t, J=11.3 Hz, 1H), 2.38 (s, 3H), 2.33 (s, 3H), 2.00 (d, J=10.4 Hz, 1H), 1.79 (d, J=11.0 Hz, 1H), 1.61-1.45 (m, 2H), 1.35 (t, J=7.1 Hz, 3H), 1.27-1.21 (m, 2H), 1.00-0.89 (m, 2H).

Preparation of 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-1,2,3-triazole-4-carboxamide

Ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-1,2,3-triazole-4-carboxylate (0.11 g, 1.0 eq.) was dissolved in anh. MeOH (1.0 mL) and 7 N ammonia in methanol (0.23 mL, 10.0 eq.) was added and the reaction mixture was stirred at 45° C. overnight. Then the solvent was evaporated and the residue was purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.073 g, 73% yield) as a yellow solid. ESI-MS: 622.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 9.15 (d, J=1.8 Hz, 1H), 8.46 (d, J=6.1 Hz, 1H), 8.29 (d, J=5.0 Hz, 1H), 8.16-8.09 (m, 3H), 8.01 (s, 1H), 7.70 (s, 1H), 7.26-7.21 (m, 2H), 7.18 (d, J=5.1 Hz, 1H), 7.04 (d, J=8.5 Hz, 1H), 3.85-3.77 (m, 3H), 3.68 (s, 2H), 3.66-3.56 (m, 2H), 2.85-2.72 (m, 2H), 2.61 (t, J=11.5 Hz, 1H), 2.39 (s, 3H), 2.33 (s, 3H), 2.00 (d, J=10.3 Hz, 1H), 1.79 (d, J=11.1 Hz, 1H), 1.65-1.43 (m, 2H), 1.24 (d, J=7.1 Hz, 2H), 1.02-0.88 (m, 2H).

Example 61. 1-Cyclopropyl-6-fluoro-7-[4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-[4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Prop-2-yn-1-ol (0.011 mL, 1.1 eq.), sodium ascorbate (0.004 g, 0.1 eq.) and copper(II) sulfate pentahydrate (0.002 g, 0.05 eq.) were sequentially added to a solution of 7-azido-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 20 (0.10 g, 1.0 eq.) in a 3:1 mixture of methanol and water (6 mL). The resulting reaction mixture was stirred at 40° C. for 2 h. Then MeOH was evaporated and DCM was added. The organic phase was separated and the aqueous layer was washed with DCM again. The combined organic layers were dried over MgSO₄, filtered and concentrated. The residue was purified by FCC (SiHP; DCM:MeOH) to yield the product (0.068 mg, 62% yield) as an off-white solid. ESI-MS: 609.7 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.59 (d, J=2.5 Hz, 1H), 8.41 (d, J=6.2 Hz, 1H), 8.29 (d, J=5.0 Hz, 1H), 8.18-8.06 (m, 2H), 8.00 (s, 1H), 7.29-7.14 (m, 3H), 7.03 (d, J=8.5 Hz, 1H), 5.42 (t, J=5.7 Hz, 1H), 4.67 (d, J=5.5 Hz, 2H), 3.87-3.72 (m, 3H), 3.67 (s, 2H), 3.65-3.55 (m, 2H), 2.87-2.73 (m, 2H), 2.67-2.55 (m, 1H), 2.38 (s, 3H), 2.33 (s, 3H), 2.04-1.95 (m, 1H), 1.84-1.73 (m, 1H), 1.63-1.45 (m, 2H), 1.31-1.17 (m, 2H), 1.01-0.88 (m, 2H).

Example 62. 1-Cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A suspension of [1-(2-hydroxyethyl)-1H-pyrazol-4-yl]boronic acid (0.03 g, 1.0 eq.), 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.113 g, 1.0 eq.) and K₂CO₃ (0.04 g, 1.5 eq.) in a mixture of water (1.0 mL) and 1,4-dioxane (4.0 mL) was purged with argon for 10 min. Then, Pd(dppf)Cl₂*DCM (0.006 g, 0.05 eq.) was added and the resulting mixture was left stirring overnight at 80° C. Subsequently, the it was cooled to RT, water was added and the resulting mixture was extracted with DCM (3×). The combined organic layers were washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.02 g, 17% yield) as a beige solid. ESI-MS: 622.6 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.33 (d, J=2.5 Hz, 1H), 8.28 (d, J=5.1 Hz, 1H), 8.19 (d, =6.4 Hz, 1H), 8.14 (d, J=2.9 Hz, 1H), 8.11-8.08 (m, 1H), 7.89 (s, 1H), 7.85 (d, J=11.5 Hz, 1H), 7.25-7.20 (m, 2H), 7.19-7.15 (m, 1H), 7.03 (d, J=8.5 Hz, 1H), 4.96 (t, 1=5.3 Hz, 1H), 4.24 (t, J=5.5 Hz, 2H), 3.86-3.74 (m, 5H), 3.64 (s, 2H), 3.63-3.56 (m, 2H), 2.82-2.73 (m, 2H), 2.64-2.56 (m, 1H), 2.37 (s, 3H), 2.33 (s, 3H), 2.02-1.95 (m, 1H), 1.81-1.74 (m, 1H), 1.60-1.45 (m, 2H), 1.33-1.25 (m, 2H), 0.96-0.86 (m, 2H).

Example 63. 8-(2-hydroxyethoxy)-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 5-{[(2-bromophenyl)amino]methylidene}-2,2-dimethyl-1,3-dioxane-4,6-dione

A solution of 2,2-dimethyl-1,3-dioxane-4,6-dione (4.4 g, 1.5 eq.) in trimethoxymethane (50.0 mL, 24.8 eq.) was refluxed for 2 h at 115° C. under argon atmosphere. Then, the reaction mixture was cooled to RT. Subsequently, 2-bromoaniline (3.5 g, 1.0 eq.) was added and the resulting mixture was stirred at 115° C. for another 2 h. Then, the reaction mixture was concentrated in vacuo to give the crude product (4.16 g, 61% yield) as white crystals which were used in the next step without further purification. ESI-MS: 324.0 [M−H]⁻.

Preparation of 8-bromo-1,4-dihydroquinolin-4-one

A mixture of 5-{[(2-bromophenyl)amino]methylidene}-2,2-dimethyl-1,3-dioxane-4,6-dione (4.2 g, 1.0 eq.), and Dowtherm® A (40.0 mL) was heated for 2 h at 250° C. Then, the reaction mixture was cooled to RT and Hexanes (100.0 mL) was added. The mixture was filtered through a pad of silica gel and the pad was washed with Hexanes (3×150.0 mL). The filtrate was purified by FCC (SiHP; Hex:EtOAc:MeOH) to give the product (2.18 g, 75% yield) as a brown solid. ESI-MS: 225.1[M+2]

Preparation of 8-bromo-1-methyl-1,4-dihydroquinolin-4-one

8-bromo-1,4-dihydroquinolin-4-one (1.01 g, 1.0 eq.) was dissolved in anh. DMF (4.0 mL) under argon atmosphere. Then, NaH (60% dispersion in mineral oil; 0.32 g, 2.0 eq.) was added in portions with stirring and the reaction mixture was left stirring for 20 min at RT. Then, CH₃I (1.0 mL, 4.0 eq.) was added dropwise and the mixture was heated at 120° C. over the weekend at the same temperature. Afterwards, MeOH (2.0 mL) was added and the mixture was concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.89 g, 82% yield) as a beige solid. ESI-MS: 239.1 [M+2]+.

Preparation of 8-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

A microwave reaction vial was charged with a mixture of 8-bromo-1-methyl-1,4-dihydroquinolin-4-one (0.8 g, 1.0 eq.), HMT (0.84 g, 2.0 eq.) and TFA (4.5 mL, 20.0 eq.). The resulting mixture was irradiated for 15 min at 120° C. using a microwave oven. Then, additional HMT (0.42 g, 1.0 eq.) was added and microwave irradiation was continued for another 5 min at 120° C. Subsequently, the reaction mixture was transferred to a flask followed by addition of H₂O (30.0 mL) and the resulting mixture was stirred for 15 min at RT. Subsequently, the mixture was concentrated in vacuo. The residue was suspended in water and extracted with EtOAc (4×100.0 mL). The combined organic layers were dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.48 g, 57% yield) as a white solid. ESI-MS: 265.9 [M+H]⁺. ¹H NMR (300 MHz, Chloroform-d) δ 10.38 (s, 1H), 8.55 (dd, J=7.9, 1.7 Hz, 1H), 8.24 (s, 1H), 8.01 (dd, J=7.7, 1.7 Hz, 1H), 7.32 (t, J=7.8 Hz, 1H), 4.34 (s, 3H).

Preparation of 8-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.48 g, 1.05 eq.) and 8-bromo-1-methyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.41 g, 1.0 eq.) in anh. DCE (10.0 mL) was stirred for 1 h at 55° C. Then, the reaction mixture was cooled to RT and NaBH(OAc)₃ (0.87 g, 2.8 eq.) was added. The resulting mixture was stirred overnight at 55° C. Afterwards, the reaction mixture was filtered through a pad of celite and the pad was washed with DCM. The filtrate was concentrated in vacuo. The residue was suspended in NaHCO₃ aq. solution and the resulting mixture was washed with DCM. The combined organic layers were dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.52 g, 55% yield) as a yellow oil. ESI-MS: 546.3 [M+H]⁺.

Preparation of 8-(2-hydroxyethoxy)-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

CuCl₂ (0.004 g, 0.1 eq.) was added to a mixture of 8-bromo-1-methyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.2 g, 1.0 eq.), K₂CO₃ (0.13 g, 3.0 eq.) and ethylene glycol (5.0 mL) and the resulting mixture was heated at 120° C. overnight at the same temperature. Then, water (5 mL) was added and the resulting mixture was washed with EtOAc (4×50.0 mL). The combined organic layers were washed with water (2.0 mL), brine (5.0 mL), dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O: MeCN) to give the product (0.038 g, 23% yield) as a yellow solid. ESI-MS: 528.7 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.14 (d, J=3.0 Hz, 1H), 7.85 (s, 1H), 7.79 (dd, J=7.0, 2.5 Hz, 1H), 7.30-7.17 (m, 5H), 7.01 (d, J=8.5 Hz, 1H), 4.94 (t, J=5.4 Hz, 1H), 4.15-4.08 (m, 5H), 3.87-3.68 (m, 5H), 3.67-3.53 (m, 3H), 2.82-2.69 (m, 2H), 2.63-2.53 (m, 1H), 2.38 (s, 3H), 2.32 (s, 3H), 2.02-1.94 (m, 1H), 1.79-1.71 (m, 1H), 1.59-1.41 (m, 2H).

Example 64. 1-[1-Cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-imidazole-4-carboxamide

Preparation of ethyl 1-(1-cyclopropyl-6-fluoro-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)-1H-imidazole-4-carboxylate

1-Cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.050 g, 1.0 eq.), ethyl 1H-imidazole-5-carboxylate (0.027 g, 1.0 eq.) and potassium carbonate (0.054 g, 2.0 eq.) were suspended in anh. MeCN (3.0 mL) and the reaction mixture was stirred at 80° C. overnight. Then water was added and the mixture was extracted with DCM (×3). The combined organic layers were washed with brine, dried over MgSO₄, filtrated and concentrated. Crude was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.064 g, 85% yield) as a white solid. ESI-MS: 370.1 [M+H]⁺.

Preparation of ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-imidazole-4-carboxylate

A solution of ethyl 1-(1-cyclopropyl-6-fluoro-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)-1H-imidazole-4-carboxylate (0.064 g, 1.0 eq.) and (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.053 g, 1.05 eq.) in anh. DCE (2.0 mL) was stirred at RT overnight. Then the mixture was cooled to 0° C., sodium triacetoxyborohydride (0.099 g, 2.8 eq.) was added and the reaction mixture was stirred at RT overnight. The reaction was quenched by water and basified to pH 11 by the addition of 1 M aq. solution of NaOH. The mixture was washed with DCM (×3). The combined organic layers were dried over MgSO₄, filtered and concentrated. The residue was purified by FCC (SiHP; DCM:MeOH) to afford the product (0.050 g, 40% yield) as a white solid. ESI-MS: 650.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.43 (t, J=1.5 Hz, 1H), 8.33-8.27 (m, 2H), 8.25 (d, J=6.4 Hz, 1H), 8.14 (d, J=2.9 Hz, 1H), 8.07 (d, J=10.9 Hz, 1H), 7.99 (s, 1H), 7.26-7.21 (m, 2H), 7.20-7.16 (m, 1H), 7.04 (d, J=8.5 Hz, 1H), 4.30 (q, J=7.1 Hz, 2H), 3.86-3.75 (m, 3H), 3.67 (s, 2H), 3.63-3.55 (m, 2H), 2.85-2.72 (m, 2H), 2.65-2.55 (m, 1H), 2.39 (s, 3H), 2.33 (s, 3H), 1.99 (d, J=10.2 Hz, 1H), 1.78 (d, J=11.2 Hz, 1H), 1.59-1.45 (m, 2H), 1.31 (t, J=7.1 Hz, 3H), 1.29-1.22 (m, 2H), 1.00-0.89 (m, 2H).

Preparation of 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-imidazole-4-carboxamide

Ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-1H-imidazole-4-carboxylate (0.043 g, 1.0 eq.) was dissolved in anh. MeOH (1.0 mL), then 7 N ammonia solution in methanol (0.08 mL, 10.0 eq.) was added and the reaction mixture was stirred at 50° C. for 3 days. The solvent was evaporated and the residue was purified by prep-HPLC (H₂O:MeOH:NH₃) to afford the product (0.014 g, 41% yield) as a white solid. ESI-MS: 621.6 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.29 (d, J=5.1 Hz, 1H), 8.26-8.18 (m, 3H), 8.13 (d, J=3.0 Hz, 1H), 8.06 (d, J=11.0 Hz, 1H), 7.98 (s, 1H), 7.54 (s, 1H), 7.31 (s, 1H), 7.26-7.20 (m, 2H), 7.20-7.16 (m, 1H), 7.03 (d, J=8.5 Hz, 1H), 3.85-3.74 (m, 3H), 3.66 (s, 2H), 3.63-3.55 (m, 2H), 2.83-2.71 (m, 2H), 2.66-2.56 (m, 1H), 2.39 (s, 3H), 2.32 (s, 3H), 1.98 (d, J=8.1 Hz, 1H), 1.78 (d, J=10.4 Hz, 1H), 1.62-1.43 (m, 2H), 1.29-1.20 (m, 2H), 1.00-0.89 (m, 2H).

Example 65. 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of (3S)—N-[(3-methoxyphenyl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 16)

A mixture of (3S)-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 17) (6.07 g, 1.1 eq.), 2-methoxypyridine-4-carbaldehyde (4.27 g, 1.0 eq.), sodium acetate (3.83 g, 1.5 eq.) and anh. MeOH (60.0 mL) was stirred at RT under argon overnight. The mixture was cooled to 0° C. and sodium borohydride (1.30 g, 1.1 eq.) was added in portions over 15 minutes. The mixture was stirred at RT for 3 hours. Solvents were evaporated and the residue was partitioned between DCM and NaOH aq. The combined organic layers were combined, dried over anh. MgSO₄, filtrated and concentrated in vacuo. The crude material was purified on FCC (SiHP (deactivated by NH₃/DCM) DCM:MeOH) to give product (4.95 g, 99% yield) as a yellow oil. ESI-MS: 299.4 [M+H]⁺. ¹H NMR (300 MHz, DMSO-d₆) δ 8.25 (d, J=2.9 Hz, 1H), 8.06 (dd, J=5.2, 0.6 Hz, 1H), 7.92 (dd, J=4.5, 1.4 Hz, 1H), 7.26 (ddd, 1=8.5, 3.0, 1.4 Hz, 1H), 7.16 (ddd, J=8.5, 4.5, 0.7 Hz, 1H), 6.98 (dd, J=5.3, 1.3 Hz, 1H), 6.81 (t, J=1.1 Hz, 1H), 3.82 (s, 3H), 3.80-3.71 (m, 3H), 3.57 (dt, J=12.9, 4.1 Hz, 1H), 2.72 (ddd, J=12.3, 11.1, 3.0 Hz, 1H), 2.54 (d, J=3.8 Hz, 2H), 2.33 (s, 1H), 1.98-1.82 (m, 1H), 1.79-1.64 (m, 1H), 1.58-1.38 (m, 1H), 1.32-1.12 (m, 1H).

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.3 g, 1.0 eq.) and (3S)—N-[(2-methoxypyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (0.115 g, 0.3 eq.) were suspended in anh. DCE (8.0 mL). The resulting mixture was stirred at RT for 30 min. Then sodium triacetoxyborohydride (0.206 g, 0.9 eq.) was added and stirring was continued overnight. The reaction mixture was poured into ice cold water and sat. aq. NaHCO₃ was added. Product was extracted with DCM. The combined organic layers were dried over anh. Na₂SO₄ and concentrated. The residue was purified by RP-FCC (C18HP, H₂O:MeCN) to give product (0.496 g, 71% yield) as a yellow solid. ESI-MS: 548.8 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.1 g, 1.0 eq.), (3R)-Pyrrolidin-3-ol (0.022 g, 1.5 eq.), cesium carbonate (0.098 g, 1.8 eq.) and 1,4-dioxane (2.0 mL). The resulting mixture was purged with argon over 5 min, then BINAP (0.021 g, 0.2 eq.) and Pd₂(dba)₃ (0.015 g, 0.1 eq.) were added. The vessel was closed and the reaction mixture was stirred at 115° C. for 4 h. The reaction was concentrated in vacuo. The residue was dissolved in DCM and washed with water and brine. The organic layer was dried over anh. Na₂SO₄ and concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) and repurified by prep-HPLC (H₂O:MeOH:NH₃) to give the product (0.252 g, 56% yield). The compound was converted into the HCl salt using 2 M HCl in Et₂O (0.139 mL, 1.0 eq to the FB), MeOH (5.0 mL) and water (20.0 mL) to provide the product (0.038 g, 96% yield) as a yellow solid. ESI-MS: 599.6 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.35 (d, J=2.9 Hz, 1H), 8.02 (d, J=4.9 Hz, 1H), 7.92 (d, J=5.3 Hz, 1H), 7.88 (s, 1H), 7.78-7.69 (m, 2H), 7.52-7.46 (m, 1H), 6.98-6.94 (m, 2H), 6.81 (s, 1H), 4.59-4.53 (m, 1H), 4.27-4.00 (m, 5H), 3.89-3.71 (m, 6H), 3.70-3.60 (m, 1H), 3.62-3.51 (m, 1H), 3.48-3.42 (m, 1H), 3.30-3.20 (m, 2H), 3.03-2.91 (m, 1H), 2.30-2.02 (m, 4H), 2.01-1.87 (m, 1H), 1.84-1.68 (m, 1H), 1.35-1.28 (m, 2H), 1.04-0.98 (m, 2H).

Example 66. 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-3-carboxylic Acid

Preparation of ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-3-carboxylate

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.20 g, 1.0 eq.), ethyl nipecotate (0.12 g, 2.0 eq.), Cs₂CO₃ (0.25 g, 2.1 eq.) and DMF (2.0 mL). The mixture was purged with argon for 5 min, followed by the addition of BINAP (0.068 g, 0.3 eq.) and Pd₂(dba)₃*CHCl₃ (0.038 g, 0.1 eq.) and stirred at 115° C. overnight. The reaction mixture was filtered through a pad of celite and concentrated in vacuo and the residue was purified by FCC (SiHP, DCM:MeOH) to give the product (0.10 g, 38% yield) as a yellow solid. ESI-MS: 667.7 [M+H]⁺.

Preparation of 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-3-carboxylic Acid

Ethyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-3-carboxylate was dissolved in a mixture of THF and H₂O (2:1, 5.0 mL). Subsequently, lithium hydroxide (0.013 g, 5.0 eq.) was added and the reaction mixture was stirred at RT overnight. The reaction mixture was concentrated, diluted with water and neutralized using 2 M HCl solution. The mixture was concentrated and the residue was purified by RP-FCC (C18HP, H₂O:MeCN) to give product (0.025 g, 95% yield) as a yellow solid. ESI-MS: 637.3 [M−H]⁻. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.1 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 7.82 (s, 1H), 7.70 (d, J=13.4 Hz, 1H), 7.37 (d, J=7.5 Hz, 1H), 7.24-7.19 (m, 2H), 7.17 (d, J=5.2 Hz, 1H), 7.02 (d, J=8.5 Hz, 1H), 3.83-3.75 (m, 1H), 3.78-3.72 (m, 1H), 3.73-3.55 (m, 4H), 3.54-3.48 (m, 1H), 3.46-3.40 (m, 1H), 3.07-2.96 (m, 2H), 2.97-2.86 (m, 1H), 2.80-2.71 (m, 2H), 2.65-2.56 (m, 2H), 2.38 (s, 3H), 2.35-2.30 (m, 3H), 2.03-1.92 (m, 2H), 1.88-1.73 (m, 2H), 1.71-1.44 (m, 4H), 1.22-1.18 (m, 2H), 0.89 (td, J=9.9, 3.3 Hz, 2H).

Example 67. 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylic Acid

Preparation of methyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylate

A pressure vessel was charged with 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq.), methyl isonipecotate (0.052 g, 2.0 eq.), Cs₂CO₃ (0.125 g, 2.1 eq.) and DMF (5.0 mL). The mixture was purged with argon for 5 min, followed by the addition of BINAP (0.034 g, 0.3 eq.) and Pd₂(dba)₃*CHCl₃ (0.019 g, 0.1 eq.) and stirred at 115° C. overnight. The reaction mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP, DCM:MeOH) to give the product (0.082 g, 58% yield) as a yellow oil. ESI-MS: 653.7 [M+H]⁺.

Preparation of 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylic Acid

Methyl 1-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylate (0.025 g, 1.0 eq.) was dissolved in a mixture of THF and H₂O (2:1). Subsequently, lithium hydroxide (0.008 mg, 5.0 eq.) was added and the reaction mixture was stirred at RT overnight. The reaction mixture was concentrated, diluted with water and neutralized using 2 M HCl solution. DCM was added and layers were separated. Organic layer was concentrated and the residue was purified by RP-FCC (C18HP, H₂O:MeCN) to give product (0.012 g, 48% yield) as a yellow solid. ESI-MS: 639.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.1 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.81 (s, 1H), 7.69 (d, J=13.5 Hz, 1H), 7.33 (d, J=7.5 Hz, 1H), 7.23-7.19 (m, 2H), 7.17 (d, J=5.1 Hz, 1H), 7.01 (d, J=8.5 Hz, 1H), 3.81-3.69 (m, 3H), 3.61-3.56 (m, 3H), 3.54-3.46 (m, 3H), 2.93-2.84 (m, 2H), 2.80-2.70 (m, 2H), 2.62-2.54 (m, 1H), 2.43-2.36 (m, 4H), 2.32 (s, 3H), 1.99-1.92 (m, 3H), 1.80-1.68 (m, 3H), 1.57-1.45 (m, 2H), 1.24-1.17 (m, 2H), 0.92-0.83 (m, 2H).

Example 68. 1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-{octahydro-1H-pyrrolo[3,2-c]pyridin-5-yl}-1,4-dihydroquinolin-4-one

Preparation of tert-butyl 5-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-octahydro-1H-pyrrolo[3,2-c]pyridine-1-carboxylate

7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.10 g, 1.0 eq), tert-butyl octahydro-1H-pyrrolo[3,2-c]pyridine-1-carboxylate (0.083 g, 2.0 eq) and cesium carbonate (0.125 g, 2.1 eq) were dissolved in DMF (3.0 mL) argon was bubbled through the mixture for 5 min. Then, BINAP (0.034 g, 0.3 eq) and Pd₂(dba)₃*CHCl₃ (0.038 g, 0.2 eq) were added and the reaction mixture was stirred at 115° C. overnight. The reactor was cooled to room temperature and the reaction mixture was filtered through a pad of celite. The pad was washed with DCM, filtrate concentrated in vacuo and the crude product purified by FCC (SiHP; DCM:MeOH). The product was redissolved in DCM and stirred with scavenger QuadraPure MPA for 20 min. The scavenger was filtered off, filtrate was concentrated and repurified by prep-HPLC (H₂O:MeOH:NH₃) to give product (0.063 mg; yield 44% yield) as a light yellow solid. ESI-MS: 737.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-{octahydro-1H-pyrrolo[3,2-c]pyridin-5-yl}-1,4-dihydroquinolin-4-one Hydrochloride

4 M HCl in 1,4-dioxane (1.16 mL, 56.0 eq) was added to a solution of tert-butyl 5-[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-octahydro-1H-pyrrolo[3,2-c]pyridine-1-carboxylate (0.061 g, 1.0 eq) in 1,4-dioxane (8.0 mL). The reaction mixture was stirred at RT for 3 h and then poured into water and further basified with 15% NaOH aq. solution. The product mixture was washed first with DCM, then CHCl₃:iPrOH (3:1). The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated. The product mixture was purified by FCC (SiHP; DCM:MeOH) to afford product (0.04 g; yield 76% yield) as a light yellow solid. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.3 mL, 1.0 eq. to FB) and a mixture of MeOH and H₂O (5:1) as a solvent to give the product (0.036 g, 90% yield) as a light yellow solid. ESI-MS: 636.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.49 (br s, 1H), 8.28 (d, J=5.0 Hz, 1H), 8.12 (d, J=3.0 Hz, 1H), 7.81 (s, 1H), 7.71 (d, J=13.4 Hz, 1H), 7.33 (d, J=7.5 Hz, 1H), 7.23-7.19 (m, 2H), 7.16 (d, J=5.2 Hz, 1H), 7.02 (d, J=8.5 Hz, 1H), 3.81-3.77 (m, 1H), 3.74 (d, J=2.7 Hz, 2H), 3.71-3.64 (m, 1H), 3.62-3.55 (m, 3H), 3.51-3.46 (m, 1H), 3.45-3.38 (m, 1H), 3.28-3.24 (m, 2H), 3.22-3.14 (m, 1H), 3.12-3.04 (m, 1H), 2.80-2.70 (m, 2H), 2.62-2.54 (m, 1H), 2.37 (s, 3H), 2.32 (s, 3H), 2.07-1.92 (m, 4H), 1.91-1.83 (m, 1H), 1.79-1.72 (m, 1H), 1.58-1.43 (m, 2H), 1.25-1.18 (m, 2H), 0.93-0.84 (m, 2H).

Example 69. 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(1H-pyrazol-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl (3S)-3-({[7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinolin-3-yl]methyl}[(2-methylpyridin-4-yl)methyl]amino)piperidine-1-carboxylate

7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 19) (1.20 g, 1.0 eq.) and tert-butyl (3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate (Intermediate 18) (1.25 g, 1.0 eq.) were dissolved in anh. 1,2-dichloroethane (20.0 mL) and stirred overnight at RT. The solution was cooled to 0° C. and then NaBH(OAc)₃ (2.52 g, 3.0 eq.) was added in portions. The reaction mixture was warmed to RT and stirred overnight. Then, the reaction mixture was quenched with water, basified to pH 11 with 1 M NaOH and extracted with DCM (3×150.0 mL). The combined organic layers were washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The crude product was purified by FCC (SiHP; DCM:MeOH) to yield the title compound (1.74 g, 55% yield). ESI-MS: 583.3 [M+H]⁺.

Preparation of tert-butyl (3S)-3-[({7-[(3R)-3-hydroxypyrrolidin-1-yl]-4-oxo-1-(propan-2-yl)-1,4-dihydroquinolin-3-yl}methyl)[(2-methylpyridin-4-yl)methyl]amino]piperidine-1-carboxylate

Cs₂CO₃ (1.25 g, 1.8 eq.) was added to a solution of tert-butyl (3S)-3-({[7-bromo-4-oxo-1-(propan-2-yl)-1,4-dihydroquinolin-3-yl]methyl}[(2-methylpyridin-4-yl)methyl]amino)piperidine-1-carboxylate (1.70 g, 1.0 eq.) and (R)-pyrrolidin-3-ol (0.28 g, 1.5 eq.) in anh. 1,4-dioxane (40.0 mL). The solution was purged with argon for 15 min and BINAP (0.26 g, 0.2 eq.) and Pd₂(dba)₃ (0.2 g, 0.1 eq.) were added. The reaction mixture was stirred overnight at 110° C., then after cooling to RT, the reaction mixture was filtered through a pad of celite, diluted with water and extracted with DCM (3×100.0 mL). The combined organic layers were washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) affording the title compound (1.30 g, 98% yield) as a yellow foam. ESI-MS: 590.7 [M+H]⁺.

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

4 M HCl in 1,4-dioxane (5.0 mL) was added to a solution of tert-butyl (3S)-3-[({7-[(3R)-3-hydroxypyrrolidin-1-yl]-4-oxo-1-(propan-2-yl)-1,4-dihydroquinolin-3-yl}methyl)[(2-methylpyridin-4-yl)methyl]amino]piperidine-1-carboxylate (1.30 g, 1.0 eq.) in 1,4-dioxane (30.0 mL). After stirring overnight at RT, the reaction mixture was cooled to 0° C. and basified to pH 11 with 1 M NaOH aq. solution. The resulting mixture was extracted with DCM (3×100.0 mL). The combined organic layers were washed with brine, dried over anh. Na₂SO₄ and concentrated in vacuo. The crude product was purified by FCC (SiHP NH₂-functionalized; DCM:MeOH) to yield the title compound (0.73 g, 63% yield). ESI-MS: 490.5 [M+H]⁺.

Preparation of 4-iodo-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole

NaH (60% suspension in mineral oil; 0.037 g, 1.2 eq.) was added in portions to a solution of 4-iodo-1H-pyrazole (0.15 g, 1.0 eq.) in dry THF (6.0 mL) in portions at 0° C. The resulting white suspension was stirred at 0° C. for 30 min and then [2-(chloromethoxy)ethyl]trimethylsilane (0.164 mL, 1.2 eq.) was added dropwise. The suspension was warmed to RT and was stirred for 2 h. The reaction mixture was diluted with water (1.0 mL) and concentrated in vacuo. The crude product was purified by FCC (SiHP; Hex:EtOAc) to give the title compound (0.157 g, 63% yield) as a colorless liquid. ¹H NMR (300 MHz, DMSO-d₆) δ 8.09 (d, J=0.7 Hz, 1H), 7.61 (d, J=0.6 Hz, 1H), 5.39 (s, 2H), 3.63-3.41 (m, 2H), 1.14-0.68 (m, 2H), −0.05 (s, 9H).

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazol-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

4-iodo-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole (0.026 g, 1.1 eq.) and sodium tert-butoxide (0.01 g, 1.4 eq) were added to a solution of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.04 g, 1.0 eq.) in anh. 1,4-dioxane (3.0 mL). After flushing the reaction mixture with argon for 10 minutes, tBuXPhos Pd G3 (0.002 g, 0.035 eq.) was added. After stirring for 18 h at 85° C. and 3 h at 100° C., the mixture was cooled to RT and then concentrated in vacuo. The crude product was purified by FCC (SiHP; DCM:MeOH) to give the title compound (0.01 g, 14% yield) as a yellow solid. ESI-MS: 686.6 [M+H]⁺.

Preparation of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(1H-pyrazol-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one

Trifluoroacetic acid (2.0 mL) was added to a solution of 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methyl pyridin-4-yl)methyl][(3S)-1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazol-4-yl)piperidin-3-yl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one (0.017 g, 1.0 eq.) in anh. DCM (6.0 mL) at 0° C. The reaction mixture was warmed to RT. After stirring for 2 h, the mixture was concentrated in vacuo. The crude compound was dissolved in MeOH (1.0 mL) and converted to free base using aqueous NH₃ (0.5 mL). The mixture was concentrated under reduced pressure and purified by prep-HPLC (H₂O:MeOH:NH₃) to give the title compound (0.004 g, 34% yield) as a white solid. ESI-MS: 556.6 [M+H]⁺. ¹H NMR (300 MHz, Methanol-d₄) δ 8.30-8.10 (m, 2H), 7.99 (s, 1H), 7.34 (s, 2H), 7.30 (s, 1H), 7.26 (d, J=5.5 Hz, 1H), 6.82 (dd, J=9.3, 1.9 Hz, 1H), 6.47 (s, 1H), 4.97 (quint, J=6.8 Hz, 1H), 4.58 (m, 1H), 3.85 (s, 2H), 3.79 (s, 2H), 3.69-3.46 (m, 4H), 3.39-3.34 (m, 1H), 3.29-3.24 (m, 1H), 3.10-2.85 (m, 1H), 2.65 (t, J=11.0 Hz, 1H), 2.54-2.42 (m, 1H), 2.39 (s, 3H), 2.26-2.04 (m, 3H), 1.97-1.83 (m, 1H), 1.80-1.53 (m, 2H), 1.49 (d, J=6.5 Hz, 6H).

Example 70. 1-cyclopropyl-6-fluoro-7-[4-(2-hydroxyethyl)piperazin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-[4-(2-hydroxyethyl)piperazin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.1 g, 1.0 eq.), 2-(piperazin-1-yl)ethan-1-ol (0.044 g, 2.0 eq.) and Cs₂CO₃ (0.1 g, 1.8 eq.) in anh. 1,4-dioxane (3.0 mL) was purged with argon for 15 min. Subsequently, BINAP (0.021 g, 0.2 eq.) and Pd₂(dba)₃ (0.016 g, 0.1 eq.) were added. After stirring at 125° C. for 10 h, the mixture was diluted with DCM and washed with H₂O and brine. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH). The obtained sample was dissolved in MeOH and scavenger QuadraPure MPA (0.5 g) was added. After stirring at RT for 1 h, the scavenger was filtered off, the filtrate was concentrated in vacuo and the residue was re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.01 g, 9% yield) as a white solid. ESI-MS: 640.6 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.0 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 7.82 (s, 1H), 7.71 (d, J=13.5 Hz, 1H), 7.34 (d, J=7.5 Hz, 1H), 7.24-7.19 (m, 2H), 7.19-7.15 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 4.45 (t, J=5.3 Hz, 1H), 3.89-3.69 (m, 3H), 3.61 (s, 2H), 3.58-3.46 (m, 4H), 3.22-3.18 (m, 4H), 2.82-2.71 (m, 2H), 2.69-2.55 (m, 5H), 2.47 (t, J=6.3 Hz, 2H), 2.38 (s, 3H), 2.33 (s, 3H), 2.03-1.91 (m, 1H), 1.82-1.71 (m, 1H), 1.61-1.35 (m, 2H), 1.29-1.12 (m, 2H), 0.95-0.80 (m, 2H).

Example 71. 1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of 1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Sodium tert-butoxide (0.06 g, 1.5 eq.) was added to a solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.23 g, 1.0 eq.) and (morpholin-2-yl)methanol (0.048 g, 1.0 eq.) in anh. 1,4-dioxane (4.0 mL). After purging with nitrogen for 15 min, BINAP (0.077 g, 0.3 eq.) and Pd₂(dba)₃ (0.038 g, 0.1 eq.) were added to the reaction mixture. After stirring for 20 h at 95° C., the mixture was diluted with EtOAc, washed with water and brine. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The crude product was purified by FCC (SiHP; DCM:MeOH). The obtained product was dissolved in MeOH and scavenger QuadraPure MPA (0.2 g) was added. After stirring for 2 h, the scavenger was filtered off and the filtrate was concentrated in vacuo affording the title compound (0.094 g, 35% yield) as a beige solid. ESI-MS: 627.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.17 (d, J=5.2 Hz, 1H), 8.10 (d, J=2.9 Hz, 1H), 7.93 (s, 1H), 7.86 (d, J=13.6 Hz, 1H), 7.41 (d, J=7.3 Hz, 1H), 7.36 (dd, J=8.6, 3.0 Hz, 1H), 7.27-7.20 (m, 2H), 7.13 (d, J=8.6 Hz, 1H), 4.17-3.99 (m, 1H), 3.94-3.76 (m, 7H), 3.72-3.58 (m, 4H), 3.57-3.43 (m, 2H), 3.07-2.93 (m, 2H), 2.94-2.51 (m, 3H), 2.42 (s, 3H), 2.35 (s, 3H), 2.29-2.05 (m, 1H), 1.99-1.85 (m, 1H), 1.77-1.57 (m, 2H), 1.42-1.25 (m, 2H), 1.00-0.87 (m, 2H).

Example 72. and Example 73. Diastereomers A and B of 1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one: Example 72. 1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (A) and Example 73. 1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (B)

Preparation of 1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Example 72) and 1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Example 73)

1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.082 g, 1.0 eq.) was separated by chiral HPLC (IF; Hex:EtOH 25% isocratic) and obtained fractions were re-purified by FCC (SiHP; DCM:MeOH) each to give Example 72. (0.01 g, 12% yield) and Example 73. (0.009 g, 11% yield) as white solids. The configuration at the carbon within morpholine moiety was assigned randomly. Example 72. ESI-MS: 627.5 [M+H]⁺ 1H NMR (400 MHz, Methanol-d₄) δ 8.19 (d, J=5.3 Hz, 1H), 8.13 (d, J=2.9 Hz, 1H), 7.92 (s, 1H), 7.86 (d, J=13.6 Hz, 1H), 7.54 (dd, J=8.7, 2.9 Hz, 1H), 7.41 (d, J=7.3 Hz, 1H), 7.30-7.21 (m, 3H), 4.10-4.02 (m, 1H), 3.98-3.75 (m, 7H), 3.73-3.59 (m, 4H), 3.56-3.42 (m, 2H), 3.09-2.90 (m, 3H), 2.87-2.69 (m, 2H), 2.47 (s, 3H), 2.36 (s, 3H), 2.20-2.09 (m, 1H), 2.03-1.91 (m, 1H), 1.77-1.63 (m, 2H), 1.35-1.29 (m, 2H), 1.00-0.87 (m, 2H). Example 73. ESI-MS: 627.8 [M+H]⁺. 1H NMR (400 MHz, Methanol-d₄) δ 8.17 (d, J=5.2 Hz, 1H), 8.10 (d, J=2.9 Hz, 1H), 7.93 (s, 1H), 7.86 (d, J=13.6 Hz, 1H), 7.41 (d, J=7.4 Hz, 1H), 7.36 (dd, J=8.6, 3.0 Hz, 1H), 7.27-7.20 (m, 2H), 7.13 (d, J=8.6 Hz, 1H), 4.14-4.01 (m, 1H), 3.95-3.84 (m, 4H), 3.84-3.75 (m, 3H), 3.73-3.58 (m, 4H), 3.57-3.42 (m, 2H), 3.10-2.93 (m, 2H), 2.86 (t, J=11.1 Hz, 1H), 2.84-2.74 (m, 1H), 2.74-2.66 (m, 1H), 2.42 (s, 3H), 2.35 (s, 3H), 2.19-2.05 (m, 1H), 2.02-1.88 (m, 1H), 1.79-1.59 (m, 2H), 1.39-1.25 (m, 2H), 1.02-0.89 (m, 2H).

Example 74. 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-[4-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of Methyl 3-fluoropyridine-4-carboxylate

A solution of 3-fluoropyridine-4-carboxylic acid (1.0 g, 1.0 eq.) and sulfuric acid (4.2 mL, 11.0 eq.) in methanol (10.0 mL) was stirred overnight in 70° C. The residue was cooled, basified to pH 9 with sat. aq. Na₂CO₃ and washed with EtOAc. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give product (0.866 g, 76% yield) as a colorless oil. ESI-MS: 156.4 [M+H]⁺.

Preparation of methyl 3-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-1-yl]pyridine-4-carboxylate

A solution of methyl 3-fluoropyridine-4-carboxylate (0.5 g, 1.0 eq.) and (S)-3-Boc-aminopiperidine (0.94 g, 1.5 eq.) in anh. toluene (7.0 mL) was heated at 120° C. for 24 h. The solvent was evaporated in vacuo. The crude product was purified by FCC (SiHP; Hex:EtOAc) to give product (0.72 g, 66% yield) as a yellow oil. ESI-MS: 336.4 [M+H]⁺.

Preparation of {3-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-4-yl}methanol

A solution of methyl 3-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-1-yl]pyridine-4-carboxylate (0.58 g, 1.0 eq.) in anh. THF (10.0 mL) was cooled in an ice bath to 0° C. and 1 M LAH in THF (2.16 mL, 1.3 eq.) was slowly added. The reaction mixture was stirred at RT for 3 h and quenched by adding MeOH and H₂O. Then 4 M HCl in 1,4-dioxane (4.2 mL) was added and the reaction mixture was stirred at 60° C. for 1 h. The solvent was evaporated, crude material was dissolved in DCM and TEA was added. The precipitated inorganic solid was filtered off, solvent was removed in vacuo and anh. MeOH (20.0 mL), NaOAc (0.272 g, 2.0 eq.) and 2-methylpyridine-4-carbaldehyde (0.24 mL, 1.3 eq.) were added. This reaction was stirred overnight and then NaBH₄ (0.094 g, 1.5 eq.) was added. Reaction was stirred 1 h at RT, then MeOH was evaporated and crude mixture was purified on RP-FCC (C18HP; H₂O:MeCN) to give product (0.186 g, 35% yield) as colorless oil. ESI-MS: 313.4 [M+H]⁺.

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-[4-(hydroxymethyl)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A solution of {3-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-4-yl}methanol (0.186 g, 1.0 eq.) and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.187 g, 1.1 eq.) in anh. DCE (10.0 mL) was stirred at 60° C. for 7 h. Then the reaction was cooled to 0° C., STAB (0.35 g, 2.8 eq.) was added and the reaction mixture was stirred overnight at RT. The crude mixture was washed with DCM and NaOH aq., the organic layer was dried over anh. Na₂SO₄, filtered and concentrated. The crude material was purified on RP-FCC (C18HP; H₂O:MeCN) to give product (0.22 g, 65% yield) as white solid. ESI-MS: 562.7 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) 8.29-8.22 (m, 3H), 8.15 (d, J=6.2 Hz, 1H), 7.96 (d, J=9.4 Hz, 1H), 7.89 (s, 1H), 7.43 (d, J=4.9 Hz, 1H), 7.18 (s, 1H), 7.12 (dd, J=5.4, 1.4 Hz, 1H), 5.30 (t, J=5.7 Hz, 1H), 4.55-4.41 (m, 2H), 3.80-3.68 (m, 2H), 3.68-3.56 (m, 2H), 3.55-3.47 (m, 1H), 3.29-3.18 (m, 1H), 2.95 (d, J=11.4 Hz, 1H), 2.87-2.74 (m, 2H), 2.73-2.62 (m, 1H), 2.35 (s, 3H), 2.07-1.94 (m, 1H), 1.86-1.71 (m, 1H), 1.62-1.43 (m, 2H), 1.27-1.14 (m, 2H), 0.97-0.79 (m, 2H).

Example 75. 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one

Preparation 2-[(1-cyclopropyl-6-fluoro-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)oxy]ethyl Acetate (Intermediate 22)

A mixture of ethane-1,2-diol (0.082 mL, 1.3 eq.), 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.3 g, 1.0 eq.), NaH (60% dispersion in mineral oil; 0.059 g, 1.3 eq.) and anh. DMF (3.0 mL) was stirred for 1 h at RT and then for 2 h at 60° C. Subsequently, the reaction mixture was concentrated in vacuo and the residue was suspended in anh. pyridine (10.0 mL). Ac₂O (0.21 mL, 2.0 eq.) was added to the above suspension followed by DMAP (0.007 g, 0.05 eq.) and the resulting mixture was stirred overnight at RT. Then, the mixture was concentrated in vacuo and the residue was partitioned between sat. NaHCO₃ aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by two consecutive FCC (SiHP; DCM:MeOH) to give the product (0.12 g, 27% yield) as an off-white solid. ESI-MS: 334.3 [M+H]⁺.

Preparation of tert-butyl N-[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl]carbamate

A suspension of tert-butyl N-[(3S,5S)-5-fluoropiperidin-3-yl]carbamate (0.99 g, 1.3 eq.), 5-bromo-2-methylpyridine (0.6 g, 1.0 eq.) and Cs₂CO₃ (1.5 g, 1.4 eq.) in anh. 1,4-dioxane (20.0 mL) was purged with argon for 10 min. Then, Xantphos (0.12 g, 0.06 eq.) and Pd₂(dba)₃ (0.16 g, 0.05 eq.) were added and the resulting mixture was purged with argon for an additional 10 min. The vessel was sealed and the reaction mixture was stirred for 3 days at 120° C. Subsequently, the mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) and fractions containing the product were combined and concentrated in vacuo. To the residue dissolved in DCM was added scavenger QuadraPure MPA (0.3 g) and the resulting mixture was stirred overnight at RT. Then, the scavenger was filtered off, washed with DCM and the filtrate was concentrated in vacuo to give the product (0.38 g, 33% yield) as a yellow solid. ESI-MS: 310.6 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-amine

4 M HCl solution in 1,4-dioxane (1.42 mL, 5.0 eq.) was added to a solution of tert-butyl N-[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl]carbamate (0.38 g, 1.0 eq.) in 1,4-dioxane (7.0 mL) and the resulting mixture was stirred for 1 h at 60° C. Then, the reaction mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The combined organic layers were dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the crude product (0.23 g, 84% yield) as a brown oil which was used in the next step without further purification. ESI-MS: 210.3 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

A mixture of (3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-amine (0.23 g, 1.0 eq.), NaOAc (0.078 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.12 mL, 1.1 eq.) in anh. MeOH (7.0 mL) was stirred overnight at RT. Then, NaBH₄ (0.047 g, 1.3 eq.) was added and the resulting mixture was left stirring for 1 h at RT. Subsequently, the reaction mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.26 g, 85% yield) as a pale yellow oil. ESI-MS: 315.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one

A mixture of (3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.09 g, 1.0 eq.), 2-[(1-cyclopropyl-6-fluoro-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)oxy]ethyl acetate (Intermediate 22) (0.11 g, 1.0 eq.) and DCE (8.0 mL) was stirred for 6 h at 60° C. Then, the mixture was cooled to 0° C., NaBH(OAc)₃ (0.17 g, 2.8 eq.) was added and the resulting mixture was stirred over the weekend at RT. Subsequently, the reaction mixture was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was dissolved in a mixture of H₂O and MeOH (10.0 mL, 1:2) and LiOH*H₂O (0.048 g, 4.0 eq.) was added. The resulting mixture was stirred for 1 h at 50° C. Then, the reaction mixture was concentrated in vacuo and the residue was purified by RP-FCC (C18HP; H₂O:MeCN) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.054 g, 32% yield) as a white solid. ESI-MS: 590.5 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J=5.0 Hz, 1H), 8.14 (d, J=3.0 Hz, 1H), 7.85 (s, 1H), 7.80 (d, J=11.7 Hz, 1H), 7.51 (d, J=7.3 Hz, 1H), 7.26-7.20 (m, 2H), 7.20-7.14 (m, 1H), 7.02 (d, J=8.5 Hz, 1H), 5.14-4.93 (m, 2H), 4.28-4.18 (m, 2H), 3.93-3.70 (m, 6H), 3.70-3.56 (m, 2H), 3.56-3.46 (m, 1H), 3.16-3.02 (m, 1H), 3.00-2.78 (m, 2H), 2.38 (s, 3H), 2.32 (s, 3H), 2.29-2.13 (m, 1H), 2.01-1.78 (m, 1H), 1.36-1.15 (m, 2H), 1.00-0.78 (m, 2H).

Example 76. 1-cyclopropyl-7-[(4R)-4-hydroxypyrrolidin-2-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl (4R)-2-(1-cyclopropyl-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)-4-hydroxypyrrolidine-1-carboxylate

7-Bromo-1-cyclopropyl-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 16) (0.3 g, 1.0 eq.), trans-N-(tert-Butoxycarbonyl)-4-hydroxy-L-proline (0.59 g, 2.5 eq.), powdered cesium carbonate (0.67 g, 2.0 eq.) and 4,4′-di-tert-butyl-2,2′-bipyridine (0.041 g, 0.15 eq.) were dissolved in anh. DMF (7.0 mL), Nickel (II) chloride ethylene glycol dimethyl ether complex (0.045 g, 0.2 eq.) and [4,4′-Bis(1,1-dimethylethyl)-2,2′-bipyridine-N1,N1′]bis[3,5-difluoro-2-[5-(trifluoromethyl)-2-pyridinyl-N]phenyl-C]Iridium(III) hexafluorophosphate (0.01 g, 0.01 eq.) were added to the resulting suspension and the green reaction mixture was put in a photoreactor with the following parameters—stirring speed=650 rpm, LED %=70%—for 1 h. After the reaction completed, the solvents were evaporated in vacuo and the residue was purified by FCC (SiHP Hex:EtOAc:MeOH) to give product (0.287 g, 67% yield) as a white solid. ESI-MS: 399.6 [M+H]⁺.

Preparation of tert-butyl (4R)-2-[1-cyclopropyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-4-hydroxypyrrolidine-1-carboxylate

A solution of tert-butyl (4R)-2-(1-cyclopropyl-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)-4-hydroxypyrrolidine-1-carboxylate (0.25 g, 1.0 eq.) and (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.19 g, 1.0 eq.) in anh. DCE (8.0 mL) was stirred at 55° C. for 3 h. The reaction mixture was cooled to RT and STAB (0.36 g, 2.8 eq.) was added. The resulting suspension was stirred overnight at RT. The reaction mixture was filtered through a pad of celite, washed with DCM (50.0 mL) and MeOH (50.0 mL). The filtrate was concentrated in vacuo. The crude was purified using FCC (SiHP; DCM:MeOH) to give product (0.20 g, 48% yield) as a white solid. ESI-MS: 679.8 [M+H]⁺.

Preparation of 1-cyclopropyl-7-[(4R)-4-hydroxypyrrolidin-2-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

tert-Butyl (4R)-2-[1-cyclopropyl-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]-4-hydroxypyrrolidine-1-carboxylate (0.18 g, 1.0 eq.) was dissolved in DCM (5.0 mL) and trifluoroacetic acid (0.40 mL, 21.0 eq.) was added dropwise. The resulting mixture was stirred overnight at RT. Solvents were evaporated in vacuo. The residue was dissolved in water and pH of the solution was adjusted to 8 by adding 1 N NaOH aq. solution. The aqueous layer was extracted with DCM (70.0 mL×4). The combined organic layers were washed with water and brine, dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (C18HP H₂O:MeCN) to give the product as a mixture of diastereomers (82:15). (0.082 g, 54% yield) as white solid. ESI-MS: 579.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.27 (dd, J=8.4, 2.8 Hz, 1H), 8.20-8.13 (m, 1H) J, 8.08 (d, J=2.9 Hz, 1H), 8.06-7.95 (m, 2H), 7.45 (dd, J=8.4, 1.6 Hz, 1H), 7.34 (dd, J=8.6, 3.0 Hz, 1H), 7.27-7.19 (m, 2H), 7.10 (d, J=8.6 Hz, 1H), 4.61-4.48 (m, 2H), 3.89 (s, 1H), 3.85 (s, 2H), 3.81 (s, 2H), 3.60 (d, J=12.2 Hz, 1H), 3.53-3.44 (m, 1H), 3.40 (dd, J=11.9, 5.2 Hz, 1H), 3.01-2.92 (m, 2H), 2.85 (t, J=11.1 Hz, 1H), 2.76-2.63 (m, 1H), 2.40 (s, 3H), 2.32 (s, 3H), 2.30-2.22 (m, 1H), 2.19-2.09 (m, 1H), 2.01-1.87 (m, 2H), 1.73-1.60 (m, 2H), 1.36-1.24 (m, 2H), 0.99-0.84 (m, 2H).

Example 77. 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetamide

Preparation of methyl 2-(3-formyl-4-oxo-7,4-dihydroquinolin-7-yl)acetate

A mixture of 4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 1)(0.2 g, 1.0 eq.), PTSA*H₂O (0.37 g, 2.0 eq.) and MeOH (10.0 mL) was heated at 80° C. overnight. Then, the mixture was concentrated in vacuo. The residue was dissolved in DMF (2.0 mL) and K₂CO₃ (0.27 g, 2.0 eq.) was added. The resulting mixture was stirred for 5 min at RT and then methyl bromoacetate (0.14 mL, 1.5 eq.) was added. The resulting mixture was heated for 1 h at 60° C. Afterwards, the mixture was partitioned between DCM and water and the aqueous layer was further washed with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP, Hex:EtOAc:DCM) to give the product (0.10 g, 42% yield) as a yellow solid. ESI-MS: 246.1 [M+H]⁺.

Preparation of methyl 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetate

A mixture of (3S)-1-(6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (Intermediate 2) (0.115 g, 1.0 eq.), methyl 2-(3-formyl-4-oxo-1,4-dihydroquinolin-1-yl)acetate (0.094 g, 1.0 eq.) in anh. DCE (5.0 mL) was heated for 1 h at 60° C., then at 40° C. for 17 h. Afterwards, the mixture was cooled to RT and NaBH(OAc)₃ (0.20 g, 2.5 eq.) was added and the resulting mixture was heated for 5 h at 40° C. Then, the mixture was partitioned between water and DCM. The aq. layer was additionally washed with DCM. The combined organic layers were washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP-15 μm, DCM:MeOH) to give the product (0.107 g, 53% yield) as a yellow semi-solid. ESI-MS: 526.6 [M+H]⁺.

Preparation of 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetic Acid

A mixture of methyl 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetate (0.107 g, 1.0 eq.), LiOH*H₂O (0.03 g, 3.6 eq.), methanol (5.0 mL) and H₂O (1.0 mL) was stirred for 2 h at 50° C. and then overnight at RT. The reaction mixture was concentrated in vacuo. 1 M HCl aq. solution (0.7 mL) was added to the above residue and the sample was purified using RP-FCC (C18HP, H₂O:MeCN) to give the product (0.105 g, quant. yield) as a yellow solid. ESI-MS: 512.4 [M+H]⁺.

Preparation 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetamide

DIPEA (0.18 mL, 5.0 eq.) was added to a mixture of 2-[3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-1-yl]acetic acid (0.105 g, 1.0 eq.), EDC hydrochloride (0.058 g, 1.5 eq.), HOBt (0.047 g, 1.5 eq.) and DMF (5.0 mL) and was followed by the addition of NH₄Cl (0.022 g, 2.0 eq.) and the resulting mixture was stirred overnight at RT. Then 0.5 M NH₃ solution in 1,4-dioxane (1.22 mL, 3.0 eq.) was added and after 160 min additionally EDC hydrochloride (0.058 g, 1.5 eq.) and HOBt (0.047 g, 1.5 eq.) were also added and the reaction mixture was stirred for 5 days at RT. Then, the mixture was concentrated in vacuo and the residue was partitioned between water and DCM. The product was found in the aqueous layer which was then concentrated in vacuo. The residue was purified by RP-FCC (C18HP, H₂O:MeCN), FCC (SiHP, DCM:MeOH) and finally prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.034 g, 33% yield) as a white solid. ESI-MS: 511.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.19 (dd, J=8.0, 1.6 Hz, 1H), 8.13 (d, J=3.0 Hz, 1H), 8.05 (s, 1H), 7.82 (s, 1H), 7.68 (ddd, J=8.7, 7.0, 1.7 Hz, 1H), 7.47-7.32 (m, 3H), 7.27 (s, 1H), 7.25-7.18 (m, 2H), 7.01 (d, J=8.5 Hz, 1H), 4.96 (s, 2H), 3.87-3.52 (m, 6H), 2.80-2.65 (m, 2H), 2.62-2.53 (m, 1H), 2.39 (s, 3H), 2.32 (s, 3H), 2.05-1.94 (m, 1H), 1.81-1.69 (m, 1H), 1.59-1.37 (m, 2H).

Example 78. 1-Cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl (3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate (Intermediate 18)

A dry reaction vessel, flushed with argon, was charged with tert-butyl (3S)-3-aminopiperidine-1-carboxylate (1.8 mL, 1.1 eq.), 2-methylpyridine-4-carbaldehyde (1.0 g, 1.0 eq.) and NaOAc (0.68 g, 1.0 eq.). Then, MeOH (8.0 mL) was added and the resulting mixture was stirred overnight at RT under argon atmosphere. Subsequently, the mixture was cooled to 0° C., NaBH₄ (0.34 g, 1.1 eq.) was added in portions and the reaction mixture was left stirring for 1.5 h at RT. Afterwards, the mixture was concentrated in vacuo and the residue was dissolved in DCM. Then, water was added and pH was adjusted to 12 using 12 M NaOH aq. solution. The resulting mixture was washed with DCM (3×). The combined organic layers were washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by two consecutive FCC (SiHP; DCM:MeOH) to give the product (2.3 g, 91% yield) as an orange oil. ESI-MS: 306.5 [M+H]⁺.

Preparation of tert-butyl (3S)-3-{[(7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate

A mixture of tert-butyl (3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate (Intermediate 18)(0.22 g, 1.1 eq.) and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.2 g, 1.0 eq.) and anh. DCE (4.0 mL) were heated for 2 h at 60° C. under inert atmosphere. Then, the mixture was cooled to RT, NaBH(OAc)₃ (0.38 g, 3.0 eq.) was added and the resulting mixture was stirred overnight at RT. Subsequently, the reaction mixture was diluted with water and partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.19 g, 56% yield) as a white solid. ESI-MS: 577.6 [M+Na]⁺.

Preparation of tert-butyl (3S)-3-[({1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-4-oxo-1,4-dihydroquinolin-3-yl}methyl)[(2-methylpyridin-4-yl)methyl]amino]piperidine-1-carboxylate

A mixture of tert-butyl (3S)-3-{[(7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidine-1-carboxylate (0.19 g, 1.0 eq.), (3R)-pyrrolidin-3-ol (0.043 g, 1.5 eq.), Cs₂CO₃ (0.2 g, 1.8 eq.) and anh. DMF (3.0 mL) was purged with argon for 15 min. Then, BINAP (0.041 g, 0.2 eq.) and Pd₂(dba)₃ (0.03 g, 0.1 eq.) were added and the resulting mixture was purged with argon for another 5 min. The vessel was sealed and the mixture was heated for 22 h at 110° C. Subsequently, the reaction mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.20 g, 96% yield). ESI-MS: 606.5 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 28)

TFA (2.0 mL, 82 eq.) was added to a solution of tert-butyl (3S)-3-[({1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-4-oxo-1,4-dihydroquinolin-3-yl}methyl)[(2-methylpyridin-4-yl)methyl]amino]piperidine-1-carboxylate (0.2 g, 1.0 eq.) in DCM (4.5 mL) and the resulting mixture was stirred for 30 min at RT. Then, the mixture was concentrated in vacuo, triturated with DCM and again concentrated in vacuo (3 cycles). The residue was partitioned between water and DCM, then aqueous layer basified with 1 M NaOH aq. solution and washed with DCM again. The combined organic layers were dried over anh. MgSO₄, filtered and concentrated in vacuo to give the product (0.133 g, 71% yield) as a yellow oil. ESI-MS: 506.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A dry reaction flask equipped with a septum was charged with 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.09 g, 1.0 eq.), 3-bromopyridine (0.035 g, 1.5 eq.), NaO t-Bu (0.029 g, 2.0 eq.) and anh. 1,4-dioxane (2.0 mL). The resulting mixture was purged with argon for 10 min and tBuXPhos-Pd-G3 (0.012 g, 0.1 eq.) was added under argon atmosphere. The vessel was capped and the reaction mixture was stirred overnight at 115° C. Subsequently, the mixture was diluted with EtOAc and filtered through celite. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.004 g, 5% yield) as a white solid. ESI-MS: 583.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.30-8.19 (m, 1H), 8.15 (d, J=5.5 Hz, 1H), 7.97-7.86 (m, 1H), 7.82 (s, 1H), 7.76 (d, J=14.8 Hz, 1H), 7.41 (ddd, J=8.6, 3.0, 1.3 Hz, 1H), 7.33-7.15 (m, 3H), 6.91 (d, J=7.7 Hz, 1H), 4.56-4.50 (m, 1H), 4.01-3.91 (m, 1H), 3.90-3.65 (m, 7H), 3.64-3.56 (m, 1H), 3.54-3.46 (m, 1H), 3.45-3.33 (m, 1H), 3.01-2.83 (m, 2H), 2.82-2.64 (m, 1H), 2.34 (s, 3H), 2.21-1.98 (m, 3H), 1.98-1.85 (m, 1H), 1.78-1.58 (m, 2H), 1.41-1.17 (m, 2H), 1.01-0.78 (m, 2H).

Example 79. 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

A solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.2 g, 1.0 eq.), (pyrrolidin-3-yl)methanol (0.053 g, 1.5 eq.) and Cs₂CO₃ (0.21 g, 1.8 eq.) in anh. DMF (6.0 mL) was purged with argon for 15 min. Then, BINAP (0.044 g, 0.2 eq.) and Pd₂(dba)₃ (0.032 g, 0.1 eq.) were added and the reaction mixture was stirred overnight at 110° C. The reaction mixture was filtered through a pad of celite. The filtrate was sonicated with scavenger QuadraPure MPA, filtered through a pad of cotton and concentrated in vacuo. The crude material was purified by FCC (SiHP; DCM:MeOH) and re-purified by prep. HPLC (H₂O:MeCN:FA) to give the desired product (0.069 g, 31% yield) as a yellow solid. ESI-MS: 611.5 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.06 mL, 1.0 eq. to FB) and DCM as a solvent (5.0 mL) to give the product (0.071 g, 95% yield) as an orange solid. ESI-MS: 611.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.28 (d, J=5.7 Hz, 1H), 8.23 (d, J=3.0 Hz, 1H), 7.99 (dd, J=9.0, 3.0 Hz, 1H), 7.86 (s, 1H), 7.72 (d, J=14.7 Hz, 1H), 7.66-7.52 (m, 3H), 6.92 (d, J=7.6 Hz, 1H), 4.22-4.07 (m, 3H), 4.05-3.84 (m, 2H), 3.82-3.75 (m, 1H), 3.75-3.55 (m, 5H), 3.46-3.37 (m, 2H), 3.22-3.06 (m, 2H), 2.95-2.85 (m, 1H), 2.62-2.51 (m, 4H), 2.46 (s, 3H), 2.27-2.09 (m, 2H), 2.05-1.94 (m, 1H), 1.89-1.63 (m, 3H), 1.38-1.24 (m, 2H), 1.03-0.90 (m, 2H).

Example 80. 7-(aminomethyl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation 2-({[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]methyl}carbamoyl)benzoic Acid

A suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 3) (0.2 g, 1.0 eq.) and potassium [(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)methyl]trifluoroboranuide (0.11 g, 1.2 eq.) in the mixture of 1,4-dioxane and water (4.5 mL, 2:1 v/v) was treated with Cs₂CO₃ (0.27 g, 2.4 eq.). The mixture was purged with argon for 15 min. Subsequently, SPhos (0.017 g, 0.12 eq.) and Pd(OAc)₂ (0.004 g, 0.052 eq.) were added. After stirring at 100° C. for 19 h, additional portions of SPhos (0.017 g, 0.12 eq.), Pd(OAc)₂ (0.004 g, 0.052 eq.) were added. The stirring was continued overnight at 110° C. The mixture was concentrated in vacuo, and the residue was dissolved in EtOAc and washed with H₂O and brine. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) affording the product (0.256 g, 17% yield). ESI-MS: 689.5 [M+H]⁺.

Preparation of 7-(aminomethyl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

1-Propanol (1.0 mL) and ethane-1,2-diamine (0.084 mL, 19.0 eq.) were added to a solution of 2-({[1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-4-oxo-1,4-dihydroquinolin-7-yl]methyl}carbamoyl)benzoic acid (0.256 g, 1.0 eq.) in 1,4-dioxane (1.0 mL). After stirring at 115° C. for 3 h under microwave irradiation, the mixture was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) followed by purification by RP-FCC (C18HP; H₂O:MeCN) affording the product (0.021 g, 61% yield) as a white solid. ESI-MS: 541.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.18-8.11 (m, 2H), 8.08 (d, J=2.9 Hz, 1H), 8.00 (s, 1H), 7.89 (d, J=10.5 Hz, 1H), 7.35 (dd, J=8.6, 3.0 Hz, 1H), 7.28-7.23 (m, 1H), 7.23-7.18 (m, 1H), 7.11 (d, J=8.6 Hz, 1H), 4.07 (s, 2H), 3.92-3.84 (m, 3H), 3.81 (s, 2H), 3.66-3.56 (m, 1H), 3.56-3.46 (m, 1H), 3.01-2.91 (m, 1H), 2.85 (t, J=11.1 Hz, 1H), 2.78-2.59 (m, 1H), 2.40 (s, 3H), 2.34 (s, 3H), 2.18-2.07 (m, 1H), 2.02-1.86 (m, 1H), 1.75-1.58 (m, 2H), 1.51-1.26 (m, 2H), 1.04-0.89 (m, 2H).

Example 81. 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl]carbamate

A suspension of tert-butyl N-[(3S)-piperidin-3-yl]carbamate (0.60 g, 1.3 eq.), 2-bromo-5-methylpyrazine (0.4 g, 1.0 eq.), Cs₂CO₃ (1.0 g, 1.4 eq.) in anh. 1,4-dioxane (10.0 mL) was purged with nitrogen for 10 min. Then, Xantphos (0.08 g, 0.06 eq.) and Pd₂(dba)₃ (0.11 g, 0.05 eq.) were added and the resulting mixture was purged again with nitrogen for 10 min. The vessel was closed and the reaction mixture was heated overnight at 100° C. Subsequently, the mixture was filtered through a pad of celite and the pad was washed with EtOAc. The filtrate was concentrated in vacuo and the residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.71 g, 87% yield) as a brown solid. ESI-MS: 293.3 [M+H]⁺.

Preparation of (3S)-1-(5-methylpyrazin-2-yl)piperidin-3-amine Hydrochloride

4 M HCl solution in 1,4-dioxane (2.5 mL, 5.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl]carbamate (0.71 g, 1.0 eq.) in 1,4-dioxane (10.0 mL). The resulting mixture was heated overnight at 40° C. Subsequently, the reaction mixture was concentrated in vacuo and the residue was purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.48 g, 97% yield) as a brown oil. ESI-MS: 193.1 [M+H]⁺.

Preparation of (3S)-1-(5-methylpyrazin-2-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

A mixture of (3S)-1-(5-methylpyrazin-2-yl)piperidin-3-amine hydrochloride (0.48 g, 1.0 eq.), NaOAc (0.16 g, 1.0 eq.) and 2-methylpyridine-4-carbaldehyde (0.24 mL, 1.1 eq.) in anh. MeOH (10.0 mL) was stirred overnight at RT. Then, NaBH₄ (0.10 g, 1.3 eq.) was added and the resulting mixture was stirred for 1 h at RT. Subsequently, the reaction mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by RP-FCC (C18HP; H₂O:MeCN). The isolated sample was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was re-purified by FCC (SiHP; DCM:MeOH) to give the product (0.14 g, 22% yield) as a brown oil. ESI-MS: 298.3 [M+H]⁺.

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of (3S)-1-(5-methylpyrazin-2-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.13 g, 1.1 eq.), 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.11 g, 1.0 eq.) and anh. DCE (3.0 mL) was stirred overnight at 50° C. Then, NaBH(OAc)₃ (0.24 g, 2.8 eq.) was slowly added in portions and the resulting mixture was stirred for 2 h at RT. Subsequently, the reaction mixture was partitioned between NaOH aq. solution and DCM. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.029 g, 11% yield) as a colorless oil. ESI-MS: 547.7 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

NaO t-Bu (0.009 g, 2.1 eq.) was added to a solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.03 g, 1.0 eq.) and (3S)-piperidin-3-ol hydrochloride (0.012 g, 2.0 eq.) in 1,4-dioxane (2.0 mL) and the resulting mixture was purged with argon for 15 min. Subsequently, BINAP (0.008 g, 0.3 eq.) and Pd₂(dba)₃ (0.004 g, 0.1 eq.) were added and the reaction mixture was stirred overnight at 100° C. Then, additional (3S)-piperidin-3-ol hydrochloride (0.006 g, 1.0 eq.), Pd₂(dba)₃ (0.004 g, 0.1 eq.) and BINAP (0.008 g, 0.3 eq.) were added and stirring was continued for 2 h. Afterwards, the reaction mixture was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH). The obtained sample was dissolved in MeOH and scavenger QuadraPure MPA (0.5 g) was added. After stirring for 1 h, the scavenger was filtered off and the filtrate was concentrated in vacuo. The residue was additionally purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.021 g, 77% yield) as a beige solid. ESI-MS: 612.6 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.18 (d, J=5.1 Hz, 1H), 8.09 (d, J=1.5 Hz, 1H), 7.97-7.91 (m, 2H), 7.82 (d, J=13.6 Hz, 1H), 7.41 (d, J=7.4 Hz, 1H), 7.29-7.21 (m, 2H), 4.57-4.48 (m, 1H), 4.27-4.11 (m, 1H), 3.94-3.74 (m, 5H), 3.71-3.62 (m, 1H), 3.54-3.41 (m, 2H), 3.12-2.99 (m, 1H), 2.97-2.69 (m, 4H), 2.36 (s, 3H), 2.35 (s, 3H), 2.23-2.13 (m, 1H), 2.12-2.01 (m, 1H), 2.01-1.87 (m, 2H), 1.87-1.68 (m, 2H), 1.67-1.43 (m, 2H), 1.36-1.21 (m, 2H), 1.03-0.84 (m, 2H).

Example 82. 7-cyclopropyl-6-fluoro-3-([{(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-7-yl]-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl]carbamate

A suspension of tert-butyl N-[(3S,5S)-5-fluoropiperidin-3-yl]carbamate (0.37 g, 1.0 eq.), 2-bromo-5-methylpyrazine (0.29 g, 1.0 eq.), Cs₂CO₃ (0.74 g, 1.4 eq.) and anh. 1,4-dioxane (6.0 mL) was purged with nitrogen for 10 min. Then, Xantphos (0.06 g, 0.06 eq.) and Pd₂(dba)₃ (0.08 g, 0.05 eq.) were added and the resulting mixture was purged with nitrogen for an additional 10 min. The vessel was closed and the reaction mixture was heated overnight at 100° C. Subsequently, the mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.37 g, 69% yield) as a brown oil. ESI-MS: 311.4 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-7-(5-methylpyrazin-2-yl)piperidin-3-amine

4 M HCl solution in 1,4-dioxane (1.5 mL, 5.0 eq.) was added to a solution of tert-butyl N-[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl]carbamate (0.37 g, 1.0 eq.) in 1,4-dioxane (5.0 mL) and the resulting mixture was heated overnight at 40° C. Then, pH was adjusted to 12 using NaOH aq. solution and the mixture was partitioned between DCM and NaOH aq. solution. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo to give the product (0.22 g, 74% yield) as a brown oil which was used in the next step without further purification. ESI-MS: 211.2 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

A mixture of (3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-amine (0.22 g, 1.0 eq.), NaOAc (0.06 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.09 mL, 1.1 eq.) and anh. MeOH (5.0 mL) was stirred overnight at RT. Then, NaBH₄ (0.04 g, 1.3 eq.) was added and the resulting mixture was stirred for 1 h at RT. Subsequently, the mixture was concentrated in vacuo and the residue was partitioned between NaOH aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH). The unreacted starting amine was isolated and the reaction was performed again in the same manner. Both isolated after purifications samples were combined to give the product (0.12 g, 47% yield) as a brown oil. ESI-MS: 316.3 [M+H]⁺.

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.1 g, 1.0 eq.), (3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.131 g, 1.05 eq.) and anh. DCE (3.0 mL) was stirred overnight at 50° C. Then, NaBH(OAc)₃ (0.22 g, 2.8 eq.) was added slowly in portions and the resulting mixture was stirred for 2 h at RT. Subsequently, the reaction mixture was partitioned between 2 M NaOH aq. solution and DCM. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.09 g, 38% yield) as a yellow foam. ESI-MS: 565.8 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-1-yl]-1,4-dihydroquinolin-4-one

A suspension of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.09 g, 1.0 eq.), (3S)-piperidin-3-ol hydrochloride (0.038 g, 2.0 eq.), NaO t-Bu (0.03 g, 2.1 eq.) and BINAP (0.03 g, 0.3 eq.) in anh. 1,4-dioxane (3.0 mL) was purged with nitrogen for 10 min. Then, Pd₂(dba)₃ (0.013 g, 0.1 eq.) was added and the resulting mixture was stirred overnight at 95° C. Subsequently, the reaction mixture was diluted with water and extracted with DCM (3×). The combined organic layers were washed with brine, dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to give the product (0.043 g, 49% yield) as a white solid. ESI-MS: 630.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.18 (d, J=5.2 Hz, 1H), 8.12 (s, 1H), 7.94 (d, J=10.4 Hz, 2H), 7.83 (d, J=13.5 Hz, 1H), 7.41 (d, J=7.4 Hz, 1H), 7.28-7.20 (m, 2H), 5.07 (d, J=47.1 Hz, 1H), 4.70 (d, J=12.5 Hz, 1H), 4.49 (t, J=13.2 Hz, 1H), 3.92-3.75 (m, 5H), 3.71-3.62 (m, 1H), 3.53-3.42 (m, 2H), 3.27-3.16 (m, 2H), 3.13-3.02 (m, 2H), 2.99-2.88 (m, 1H), 2.80 (dd, J=11.5, 8.8 Hz, 1H), 2.48-2.39 (m, 1H), 2.38-2.33 (m, 6H), 2.15-1.90 (m, 3H), 1.83-1.69 (m, 1H), 1.56-1.43 (m, 1H), 1.35-1.26 (m, 2H), 1.00-0.91 (m, 2H).

Example 83. 1-cyclopropyl-6-fluoro-3-({[(3S)-7-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-7,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-yl]carbamate

A suspension of tert-butyl N-[(3S)-piperidin-3-yl]carbamate (0.27 g, 1.3 eq.), 5-bromo-3-fluoro-2-methylpyridine (0.2 g, 1.0 eq.) and Cs₂CO₃ (0.69 g, 2.0 eq.) in anh. 1,4-dioxane (4.0 mL) was purged with argon for 10 min. Then, Xantphos (0.04 g, 0.06 eq.) and Pd₂(dba)₃ (0.05 g, 0.05 eq.) were added and the resulting mixture was additionally purged with argon for 5 min. The vessel was closed and the mixture was heated overnight at 100° C. Subsequently, the reaction mixture was partitioned between water and DCM. The organic layer was dried over anh. MgSO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.30 g, 89% yield) as a beige solid. ESI-MS: 310.3 [M+H]⁺.

Preparation of (3S)-7-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-amine

TFA (2.0 mL, 28.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-yl]carbamate (0.30 g, 1.0 eq.) in DCM (6.0 mL) was added and the resulting mixture was left stirring at RT for 1 h. Then, the reaction mixture was concentrated in vacuo, triturated with MeOH and concentrated again in vacuo (3 cycles). The residue was partitioned between water and DCM. The aqueous layer was basified with 1 M NaOH aq. solution and washed with DCM. The organic layer was dried over anh. MgSO₄, filtered and concentrated in vacuo to give the product (0.17 g, 84% yield) as a yellowish oil which was used in the next step without further purification. ESI-MS: 210.1 [M+H]⁺.

Preparation of (3S)-1-(5-fluoro-6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

A mixture of 2-methylpyridine-4-carbaldehyde (0.092 mL, 1.1 eq.), (3S)-1-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-amine (0.176 g, 1.0 eq.), NaOAc (0.065 g, 1.0 eq.) and MeOH (5.0 mL) was stirred overnight at RT. Then, the reaction mixture was cooled to 0° C., NaBH₄ (0.033 g, 1.1 eq.) was added in portions and the resulting mixture was stirred overnight at RT. Subsequently, the reaction mixture was concentrated in vacuo and the residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.18 g, 71% yield) as a yellowish oil. ESI-MS: 315.2 [M+H]+

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S)-1-(5-fluoro-6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one

A mixture of (3S)-1-(5-fluoro-6-methylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.11 g, 1.0 eq.), 2-[(1-cyclopropyl-6-fluoro-3-formyl-4-oxo-1,4-dihydroquinolin-7-yl)oxy]ethyl acetate (Intermediate 22) (0.15 g, 1.1 eq.) and anh. DCE (12.0 mL) was stirred for 8 h at 60° C. Then, the reaction mixture was cooled to 0° C., NaBH(OAc)₃ (0.20 g, 2.8 eq.) was added and the resulting mixture was stirred over the weekend at RT. Afterwards, the mixture was partitioned between sat. NaHCO₃ aq. solution and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was dissolved in a mixture of H₂O and MeOH (10.0 mL, 7:3 v/v) and LiOH*H₂O (0.06 g, 4.0 eq.) was added. The resulting mixture was stirred for 2 h at 50° C. Then, the mixture was concentrated in vacuo and the residue was purified by two consecutive RP-FCC (C18HP; H₂O:MeCN) and re-purified by prep-HPLC (H₂O:MeCN:NH₃) to give the product (0.04 g, 19% yield) as an off-white solid. HR-MS: 590.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.15 (d, J=5.2 Hz, 1H), 7.97-7.91 (m, 2H), 7.89 (d, J=11.6 Hz, 1H), 7.55 (d, J=7.1 Hz, 1H), 7.25 (s, 1H), 7.23-7.18 (m, 1H), 7.15 (dd, J=12.6, 2.5 Hz, 1H), 4.35-4.26 (m, 2H), 4.02-3.96 (m, 2H), 3.95-3.74 (m, 5H), 3.69-3.60 (m, 1H), 3.53-3.43 (m, 1H), 2.98-2.85 (m, 2H), 2.81-2.69 (m, 1H), 2.41-2.23 (m, 6H), 2.18-2.07 (m, 1H), 1.96-1.83 (m, 1H), 1.76-1.57 (m, 2H), 1.37-1.23 (m, 2H), 0.99-0.87 (m, 2H).

Example 84. 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 23)

A solution of (3S)-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 17) (3.38 g, 1.0 eq.), sodium acetate (1.45 g, 1.0 eq.) and 2-methylpyridine-4-carbaldehyde (2.2 mL, 1.1 eq.) in anh. methanol (100.0 mL) was stirred overnight at RT. Then NaBH₄ (0.87 g, 1.3 eq.) was added and the reaction mixture was stirred for 1 h at RT. The crude was concentrated and the residue was partitioned between DCM and NaOH aq. solution. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (C18HP; H₂O:MeCN) to give product (3.94 g, 87% yield) as yellow oil. ESI-MS: 283.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 24)

A mixture of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 23)(1.0 g, 1.0 eq.) and 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.9 g, 1.0 eq.) in anh. DCE (13.0 mL) was stirred for 3 h at 55° C. Then, the mixture was cooled in an ice-bath and sodium triacetoxyborohydride (2.1 g, 2.8 eq.) was added in portions and stirring was continued overnight at 55° C. Subsequently, the solvent was evaporated and the residue was diluted with DCM. The organic layer was then washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The crude product was purified by FCC (SiHP; DCM:MeOH) to give the product (0.75 g, 40% yield) as a yellow solid. ESI-MS: 516.5 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one hydrochloride

1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 24) (0.1 g, 1.0 eq.) was dissolved in ethylene glycol (1.9 mL) and the mixture was cooled to 0° C. Then sodium hydride (60% dispersion in mineral oil, 0.04 g, 5.0 eq.) was added in portions. The resulting mixture was stirred overnight at 60° C. This reaction was repeated starting with 0.2 g of Intermediate 24. Subsequently, combined reaction mixtures were partitioned between water and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The crude was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP-FCC (C18HP; H₂O:MeCN) to afford product (0.22 g, 81% yield) as a yellowish solid. The compound (0.2 g) was converted to the HCl salt using 2 M HCl in Et₂O solution (0.17 mL, 1 eq. to FB) and DCM (12.0 mL) as solvent to provide the product (0.2 g, 99% yield) as an orange solid. HR-MS: 558.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄): 8.42 (d, J=2.9 Hz, 1H), 8.33 (d, J=5.9 Hz, 1H), 8.06-7.97 (m, 3H), 7.88 (d, J=11.4 Hz, 1H), 7.76-7.64 (m, 3H), 7.59 (d, J=7.1 Hz, 1H), 4.35-4.28 (m, 2H), 4.26-4.10 (m, 3H), 4.04-3.92 (m, 3H), 3.91-3.80 (m, 2H), 3.56-3.49 (m, 1H), 3.21-3.02 (m, 2H), 2.99-2.89 (m, 1H), 2.52 (s, 3H), 2.27-2.16 (m, 1H), 2.03-1.93 (m, 1H), 1.85-1.62 (m, 2H), 1.39-1.30 (m, 2H), 1.05-0.97 (m, 2H).

Example 85. 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(pyrazin-2-yl)piperidin-3-yl]carbamate

Cs₂CO₃ (2.8 g, 2.0 eq.) was added to a solution of 2-chloropyrazine (0.5 g, 1.0 eq.) and (S)-3-Boc-aminopiperidine (1.0 g, 1.2 eq.) in DMSO (8.0 mL) and the mixture was stirred 5 h at 100° C. Afterwards, the reaction mixture was cooled to RT, diluted with water and washed with Et₂O. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The product was purified by FCC (SiHP; Hex:EtOAc) to give product (0.71 g, 58% yield) as a pale yellow solid. ESI-MS: 279.1 [M+H]⁺. ¹H NMR (400 MHz, Chloroform-d) δ 8.21 (s, 1H), 8.13 (dd, J=2.5, 1.4 Hz, 1H), 7.83 (d, J=2.7 Hz, 1H), 4.69 (s, 1H), 3.96 (d, J=12.8 Hz, 1H), 3.76 (s, 2H), 3.53-3.30 (m, 2H), 2.03-1.93 (m, 1H), 1.84 (ddq, J=13.3, 6.7, 3.5 Hz, 1H), 1.75-1.57 (m, 2H), 1.47 (s, 9H).

Preparation of (3S)-1-(pyrazin-2-yl)piperidin-3-amine

Trifluoroacetic acid (1.9 mL, 10.0 eq.) was added to the solution of tert-butyl N-[(3S)-1-(pyrazin-2-yl)piperidin-3-yl]carbamate (0.7 g, 1.0 eq.) in DCM (12.0 mL) and the reaction mixture was stirred for 3 h at RT. Afterwards, the mixture was concentrated in vacuo and traces of moisture were azeotropically removed with MeOH to give product (0.73 g, 99% yield) as a trifluoroacetate salt. The product was used in the next step without further purification. ESI-MS: 179.0 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyrazin-2-yl)piperidin-3-amine (Intermediate 25)

(3S)-1-(pyrazin-2-yl)piperidin-3-amine trifluoroacetate (0.73 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.27 mL, 1.0 eq.) and sodium acetate (0.20 g, 1.0 eq.) were dissolved in anh. MeOH (8.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.19 g, 2.0 eq.) was added and the reaction mixture was stirred overnight at RT. After that time the reaction was concentrated in vacuo and residue was extracted with DCM and aq. NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. The crude product was purified by FCC (SiHP; DCM:MeOH) to give product (0.59 g, 83% yield) as a pale yellow oil. ESI-MS: 284.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.47 g, 1.0 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyrazin-2-yl)piperidin-3-amine (Intermediate 25) (0.57 g, 1.1 eq.) in anh. DCE (18.0 mL) was stirred 5 h at 60° C. Then the mixture was cooled to RT and sodium triacetoxyborohydride (1.9 g, 5.0 eq.) was added. Resulting mixture was stirred overnight at 60° C. Then the mixture was diluted with DCM and washed with 0.1 M NaOH. Aqueous layer was extracted with DCM. Combined organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. The residue was purified by FCC (SiHP; DCM:MeOH) to give desired product (0.82 g, 79% yield) as a yellow foam. ESI-MS: 517.5 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Sodium hydride (60% dispersion in mineral oil, 0.29 g, 5.0 eq.) was added in portions to the solution of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.82 g, 1.0 eq.) in ethane-1,2-diol (14.0 mL) at 0° C. The mixture was stirred at 0° C. for 1 h, warmed to RT and then the mixture was stirred overnight at 60° C. The reaction mixture was quenched by the addition of water. The mixture was diluted with water and washed with DCM. The organic layer was dried over Na₂SO₄, filtered and concentrated under reduced pressure. The crude material was purified by FCC (SiHP; DCM:MeOH) and re-purified by RP FCC (C18HP; H₂O:MeCN) and re-purified once again using FCC (SiCN column; DCM:MeOH) to give product (0.49 g, 60% yield) as a pale yellow foam. The compound was converted into the HCl salt using 2 M HCl in Et₂O and DCM as a solvent. The mixture was concentrated in vacuo and the compound was freeze-dried to give product (0.5 g, 96% yield) as a yellow solid. ESI-MS: 559.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.53 (d, J=6.0 Hz, 1H), 8.33 (d, J=1.4 Hz, 1H), 8.27 (s, 1H), 8.16 (dd, J=2.7, 1.5 Hz, 1H), 8.01 (s, 1H), 7.97-7.93 (m, 1H), 7.90 (d, J=11.3 Hz, 1H), 7.81 (d, J=2.7 Hz, 1H), 7.68 (d, J=7.1 Hz, 1H), 4.85 (d, J=12.5 Hz, 1H), 4.54 (s, 2H), 4.40-4.32 (m, 2H), 4.32-4.21 (m, 3H), 4.07-3.98 (m, 2H), 3.63 (tt, J=7.1, 3.9 Hz, 1H), 3.44-3.35 (m, 1H), 3.32-3.25 (m, 1H), 3.10 (td, J=13.3, 2.6 Hz, 1H), 2.65 (s, 3H), 2.40-2.30 (m, 1H), 2.12-1.95 (m, 2H), 1.74-1.59 (m, 1H), 1.44-1.32 (m, 2H), 1.20-1.07 (m, 2H).

Example 86. 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

(3S)-Piperidin-3-ol hydrochloride (0.14 g, 2.5 eq.) and 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 24)(0.21 g, 1.0 eq.) were dissolved in 1-methyl-2-pyrrolidinone (2.0 mL) and then TEA (0.28 mL, 5.0 eq.) was added. Reaction mixture was heated at 100° C. for 5 days. Then the reaction mixture was diluted with water and washed with EtOAc. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. The crude product was purified by FCC (SiHP; DCM:MeOH) and repurified by RP-FCC (C18HP, MeCN:H₂O) to give product (0.17 g, 71% yield) as a beige solid. ESI-MS: 597.5 [M+H]⁺. The compound was converted into the HCl salt using 2 M HCl in Et₂O and DCM as a solvent. The mixture was concentrated in vacuo and the compound was freeze-dried to give a product (0.14 g, 100% yield) as an orange solid. HR-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄): 8.47 (d, J=2.9 Hz, 1H), 8.36 (d, J=6.0 Hz, 1H), 8.15-8.10 (m, 1H), 8.06 (d, J=5.3 Hz, 1H), 8.00 (s, 1H), 7.84-7.74 (m, 4H), 7.42 (d, J=7.4 Hz, 1H), 4.29 (d, J=12.0 Hz, 1H), 4.19 (s, 2H), 4.04-3.82 (m, 4H), 3.69-3.62 (m, 1H), 3.54-3.44 (m, 2H), 3.24-3.06 (m, 2H), 3.02-2.92 (m, 2H), 2.88-2.81 (m, 1H), 2.55 (s, 3H), 2.28-2.19 (m, 1H), 2.10-1.91 (m, 3H), 1.86-1.64 (m, 3H), 1.56-1.45 (m, 1H), 1.36-1.28 (m, 2H), 1.04-0.98 (m, 2H).

Example 87. 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 26)

A mixture of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (Intermediate 23)(1.0 g, 1.0 eq.) and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (1.0 g, 1.1 eq.) in anh. DCE (30.0 mL) was stirred at 60° C. for 3 h. Then, the mixture was cooled in an ice-bath to 0° C. and sodium triacetoxyborohydride (2.1 g, 2.8 eq.) was added in portions. The reaction mixture was stirred overnight at RT, then heated to 40° C. for 1 h. AcOH (0.2 mL, 1.0 eq.) was added and reaction was stirred at RT for 1 h. Then, the mixture was cooled in an ice-bath to 0° C. and sodium triacetoxyborohydride (2.1 g, 2.8 eq.) was added in portions and stirred at 60° C. for 1 h. The solvent was evaporated and the residue was diluted with DCM and washed with sat. NaHCO₃ aq., Organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The crude product was purified by FCC (SiHP; DCM:MeOH) to give the product (0.81 g, 43% yield) as a yellow solid. ESI-MS: 532.5 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

The solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 26) (0.05 g, 1.0 eq.) and (3S)-pyrrolidin-3-yl]methanol (0.016 g, 1.8 eq.) in anh. 1,4-dioxane (1.0 mL) was purged with argon for 15 min. Then, sodium tert-butoxide (0.018 g, 2.1 eq.), BINAP (0.016 g, 0.3 eq.) and Pd₂(dba)₃ (0.008 g, 0.1 eq.) were added. The resulting mixture was heated at 95° C. overnight. The same reaction was repeated twice starting with 0.05 g and 0.28 g of Intermediate 26. Product mixtures from both the batches were combined and filtered through a pad of celite and washed further with MeOH. The filtrate was concentrated in vacuo. The product was purified by FCC (SiHP; DCM:MeOH). The residue was dissolved in MeOH and scavenger QuadraPure MPA (4.0 eq.) was added. The mixture was stirred overnight at RT. Afterwards the mixture was filtered. Filtrate was concentrated in vacuo and the residue was re-purified by FCC (SiHP; DCM:MeOH) to give a product (0.29 g, 73% yield). ESI-MS: 597.5 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.2 mL, 1.0 eq. to FB) and DCM as a solvent (3.0 mL) to give the product (0.26 g, 82% yield) as a yellow solid. ESI-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.46 (d, J=2.9 Hz, 1H), 8.32 (d, J=5.8 Hz, 1H), 8.07 (d, J=5.0 Hz, 1H), 8.02 (dd, J=8.6, 2.5 Hz, 1H), 7.91 (s, 1H), 7.78-7.68 (m, 3H), 7.65 (d, J=6.0 Hz, 1H), 6.94 (d, J=7.7 Hz, 1H), 4.33-4.15 (m, 3H), 4.05 (d, J=12.9 Hz, 1H), 3.98-3.83 (m, 2H), 3.78-3.56 (m, 5H), 3.49-3.39 (m, 2H), 3.27-3.12 (m, 2H), 2.98 (td, J=12.6, 2.7 Hz, 1H), 2.62-2.53 (m, 1H), 2.50 (s, 3H), 2.32-2.23 (m, 1H), 2.23-2.12 (m, 1H), 2.06-1.98 (m, 1H), 1.93-1.80 (m, 2H), 1.80-1.67 (m, 1H), 1.37-1.27 (m, 2H), 1.05-0.94 (m, 2H).

Example 88. 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

The solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 26) (0.39 g, 1.0 eq.) and [(3R)-pyrrolidin-3-yl]methanol (0.12 g, 1.8 eq.) in anh. 1,4-dioxane (7.0 mL) was purged with argon for 15 min. Then sodium tert-butoxide (0.14 g, 2.1 eq.), BINAP (0.13 g, 0.3 eq.) and Pd₂(dba)₃ (0.062 g, 0.1 eq.) were added. The resulting mixture was heated overnight at 95° C. The reaction mixture was filtered though a pad of celite and the filtrate was concentrated under reduced pressure. The residue was dissolved in DCM (100.0 mL) and scavenger QuadraSil MP (1.0 g) was added. The mixture was stirred overnight at RT, scavenger beans were filtered off, washed with DCM and concentrated in vacuo. The crude product was purified by RP-FCC (C18HP; H₂O:MeCN) to give desired product (0.31 g, 75% yield) as a yellow solid. ESI-MS: 597.5 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.25 mL, 1.0 eq. to FB) and DCM as a solvent (15.0 mL) to give the product (0.32 g, 98% yield) as a yellow solid. ESI-MS: 597.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄): 8.45-8.41 (m, 1H), 8.30 (d, J=5.8 Hz, 1H), 8.04 (d, J=5.2 Hz, 1H), 8.00-7.95 (m, 1H), 7.89 (s, 1H), 7.75-7.65 (m, 3H), 7.64-7.58 (m, 1H), 6.92 (d, J=7.6 Hz, 1H), 4.28-4.15 (m, 3H), 4.08-3.99 (m, 1H), 3.95-3.82 (m, 2H), 3.75-3.57 (m, 5H), 3.47-3.39 (m, 2H), 3.24-3.12 (m, 2H), 3.01-2.91 (m, 1H), 2.59-2.51 (m, 1H), 2.48 (s, 3H), 2.30-2.21 (m, 1H), 2.20-2.11 (m, 1H), 2.05-1.97 (m, 1H), 1.90-1.65 (m, 3H), 1.34-1.26 (m, 2H), 1.02-0.94 (m, 2H).

Example 89. 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl]carbamate

A suspension of 3-bromopyridine (1.8 g, 1.0 eq.), tert-butyl N-[(3S,5S)-5-fluoropiperidin-3-yl]carbamate (3.2 g, 1.3 eq.) and cesium carbonate (5.0 g, 1.4 eq.) in anh. 1,4-Dioxane (35.0 mL) was sparged with argon over 20 min and after this time Pd₂(dba)₃ (0.52 g, 0.05 eq.) and Xantphos (0.4 g, 0.06 eq.) were added. The reaction tube was closed and reaction mixture was stirred at 140° C. for 2 days. The reaction mixture was cooled to RT and sparged with argon. Then Pd₂(dba)₃ (0.52 g, 0.05 eq.) and Xantphos (0.4 g, 0.06 eq.) were added and reaction was stirred at 140° C. overnight. The crude mixture was filtered through a pad of celite. Filtrate was concentrated in vacuo. Residue was purified by FCC (SiHP; Hex:EtOAc) to give desired product. The product was dissolved in DCM (50.0 mL) and scavenger QuadraSil MP (0.3 g) was added. Mixture was stirred overnight, scavenger beans were filtrated off, washed with DCM. Solvent was concentrated to give the product (1.05 g, 29% yield) as yellow solid. ESI-MS: 296.1 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-amine Trifluoroacetate

tert-Butyl N-[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl]carbamate (1.05 g, 1.0 eq.) was dissolved in DCM (17.0 mL) and trifluoroacetic acid (2.5 mL, 10.0 eq.) was added dropwise to this solution. The reaction mixture was stirred at RT overnight. Solvent was evaporated and the residue was dissolved in MeOH and concentrated (three cycles) to azeotropically remove traces of moisture. The desired product (1.6 g, quantitative) was obtained as a brown solid. ESI-MS: 196.2 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-N-[(2-methylpyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine

2-Methylpyridine-4-carbaldehyde (0.37 mL, 1.0 eq.), (3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-amine trifluoroacetate (1.19 g, 1.0 eq.) and sodium acetate (0.27 g, 1.0 eq.) were dissolved in anh. methanol (11.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.25 g, 2.0 eq.) was added. The reaction was stirred overnight at RT. The reaction mixture was then concentrated in vacuo and residue was partitioned between DCM and aq. NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. Crude product was purified by FCC (SiHP; DCM:MeOH) to give product (0.55 g, 55% yield) as a beige solid. ESI-MS: 301.2 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.61 g, 1.3 eq.) and (3S,5S)-5-fluoro-N-[(2-methylpyridin-4-yl)methyl]-1-(pyridin-3-yl)piperidin-3-amine (0.55 g, 1.0 eq.) in anh. DCE (18.0 mL) was heated at 60° C. for 4 h. Then the mixture was cooled to 0° C. and sodium triacetoxyborohydride (1.55 g, 4.0 eq.) was added in portions. The resulting mixture was stirred for 3 days at RT. 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.23 g, 0.5 eq.) and sodium triacetoxyborohydride (0.78 g, 2.0 eq.) were then added and the reaction mixture was stirred overnight at RT. The reaction mixture was diluted with water and washed with DCM. The organic layer was washed with brine, dried over Na₂SO₄ and concentrated in vacuo. Crude product was purified by RP-FCC (C18HP; H₂O:MeCN) to give desired product (0.545 g, 51% yield) as a yellowish solid. ESI-MS: 534.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

1-cyclopropyl-6,7-difluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.545 g, 1.0 eq.) was dissolved in ethylene glycol (9.3 mL) and the mixture was cooled to 0° C. Then sodium hydride (60% dispersion in mineral oil, 0.19 g, 5.0 eq.) was added in portions. The resulting mixture was stirred at 60° C. overnight. The reaction mixture was diluted with water and extracted to DCM. The organic layer was dried over anh. Na₂SO₄ and concentrated in vacuo. The crude material was purified by RP-FCC (C18HP; H₂O:MeCN) to give pure product (0.51 g, 92% yield) as a beige solid. ESI-MS: 576.4 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.41 mL, 1.0 eq. to FB) and DCM as a solvent (25.0 mL) to give the product (0.50 g, 97% yield) as a yellow solid. ESI-MS: 576.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄): 8.45-8.38 (m, 1H), 8.34 (d, J=5.9 Hz, 1H), 8.03-7.98 (m, 2H), 7.96-7.87 (m, 2H), 7.76 (s, 1H), 7.70 (d, J=5.9 Hz, 1H), 7.65-7.57 (m, 2H), 5.13 (d, J=46.9 Hz, 1H), 4.31 (t, J=4.6 Hz, 2H), 4.28-4.14 (m, 2H), 4.07 (s, 2H), 4.01-3.97 (m, 2H), 3.92-3.86 (m, 1H), 3.79-3.70 (m, 1H), 3.56-3.50 (m, 1H), 3.39-3.32 (m, 1H), 3.28-3.13 (m, 2H), 2.55 (s, 3H), 2.52-2.41 (m, 1H), 2.14-1.93 (m, 1H), 1.36-1.29 (m, 2H), 1.04-0.94 (m, 2H).

Example 90. 1-cyclopropyl-6-fluoro-3-({[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl]carbamate

A suspension of 3-bromo-5-fluoropyridine (1.0 g, 1.0 eq.), (S)-3-Boc-aminopiperidine (1.5 g, 1.3 eq.) and cesium carbonate (2.5 g, 1.4 eq.) in anh. 1,4-dioxane (20.0 mL) was sparged with argon for 20 min and then, Pd₂(dba)₃ (0.26 g, 0.05 eq.) and Xantphos (0.33 g, 0.1 eq.) were added and the reaction mixture was stirred at 140° C. for 48 h. The crude mixture was filtered through a pad of celite, eluted with methanol. The filtrate was concentrated in vacuo. This crude product was purified on FCC (SiHP; Hex:EtOAc) and the resulting residue was dissolved in EtOAc and scavenger QuadraSil MP (1.0 g) was added. The mixture was stirred 4 h at RT. Scavenger was filtered off and washed with EtOAc. The solvent was evaporated to give the product (1.7 g, 99% yield) as a yellow solid. ESI-MS: 296.4 [M+H]⁺.

Preparation of (3S)-1-(5-fluoropyridin-3-yl)piperidin-3-amine Trifluoroacetate

Trifluoroacetic acid (4.3 mL, 10.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl]carbamate (1.7 g, 1.0 eq.) in DCM (35.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was concentrated in vacuo and traces of moisture were removed from this residue by azeotropic codistillation with MeOH to give product (1.8 g, 99% yield) as a yellow oil. ESI-MS: 196.1 [M+H]⁺. The product was used in next step without further purification.

Preparation of (3S)-1-(5-fluoropyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (3S)-1-(5-fluoropyridin-3-yl)piperidin-3-amine trifluoroacetate (1.8 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.62 mL, 1.0 eq.) and sodium acetate (0.46 g, 1.0 eq.) was dissolved in anh. methanol (20.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.42 g, 2.0 eq.) was added. The reaction mixture was stirred 3 days at RT. After that time the reaction was concentrated in vacuo and residue was partitioned between DCM and aqueous NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. Crude product was purified by FCC (SiHP; DCM:MeOH) to give product (0.95 g, 54% yield) as pale brown oil. ESI-MS: 301.2 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.62 g, 1.0 eq.) and (3S)-1-(5-fluoropyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.92 g, 1.2 eq.) in anh. 1,2-dichloroethane (25.0 mL) was stirred for 5 h at 60° C. Then the mixture was cooled to RT and sodium triacetoxyborohydride (2.6 g, 5.0 eq.) was added. Resulting mixture was stirred overnight at 60° C. After that time the mixture was diluted with DCM and washed with 0.1 M NaOH aq. solution. The aqueous layer was washed further with DCM. Combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. Crude was purified by FCC (SiHP; DCM:MeOH) to give product (0.80 g, 59% yield) as a yellow oil. ESI-MS: 534.5 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Sodium hydride (60% dispersion in mineral oil, 0.29 g, 5.0 eq.) was added in portions to a solution of 1-cyclopropyl-6,7-difluoro-3-({[(3S)-1-(5-fluoropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.80 g, 1.0 eq.) in ethylene glycol (15.0 mL) at 0° C. The mixture was stirred at 0° C. for 1 h and warmed to RT. Then the mixture was stirred overnight at 60° C. After that time the reaction mixture was diluted with water and extracted with DCM. The organic layer was dried over Na₂SO₄, filtered and concentrated in vacuo. Crude was purified twice by FCC (SiHP; DCM:MeOH) to give a product (0.77 g, 94% yield) as a yellow foam. ESI-MS: 576.4 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.67 mL, 1.0 eq. to FB) and DCM as a solvent (13.0 mL) to give the product (0.76 g, 100% yield) as a yellow solid. ESI-MS: 576.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.40 (d, J=6.0 Hz, 1H), 8.24-8.19 (m, 1H), 8.11 (s, 1H), 7.93-7.84 (m, 3H), 7.80 (d, J=5.9 Hz, 1H), 7.62 (d, J=7.1 Hz, 1H), 7.47 (dt, J=12.2, 2.3 Hz, 1H), 4.37-4.33 (m, 2H), 4.32-4.23 (m, 2H), 4.22-4.14 (m, 1H), 4.10-3.93 (m, 4H), 3.81 (d, J=12.5 Hz, 1H), 3.61-3.54 (m, 1H), 3.24-3.11 (m, 2H), 2.94 (td, J=12.5, 2.6 Hz, 1H), 2.58 (s, 3H), 2.31-2.21 (m, 1H), 2.05-1.95 (m, 1H), 1.91-1.79 (m, 1H), 1.73 (ddt, J=15.8, 12.0, 5.7 Hz, 1H), 1.42-1.31 (m, 2H), 1.11-1.01 (m, 2H).

Example 91. 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 27)

A mixture of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.51 g, 1.0 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyrazin-2-yl)piperidin-3-amine (Intermediate 25) (0.5 g, 1.0 eq.) in anh. DCE (8.0 mL) was stirred for 3 h at 60° C. Then the mixture was cooled to 0° C. and sodium triacetoxyborohydride (1.05 g, 2.8 eq.) was added. The resulting mixture was stirred overnight at 60° C. After that time the mixture was diluted with DCM and washed with water. The aqueous layer was washed again with DCM. The combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give product (0.695 g, 72% yield) as a yellow solid. ESI-MS: 533.7 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

The solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 27) (0.20 g, 1.0 eq.) and [(3R)-pyrrolidin-3-yl]methanol (0.07 g, 1.8 eq.) in anh. 1,4-dioxane (4.0 mL) was purged with argon for 15 min. Then sodium tert-butoxide (0.07 g, 2.1 eq.), BINAP (0.07 g, 0.3 eq.) and Pd₂(dba)₃ (0.03 g, 0.1 eq.) were added. The resulting mixture was heated overnight at 95° C. The reaction mixture was filtered through a pad of celite and concentrated under reduced pressure. The residue was dissolved in DCM and scavenger QuadraSil MP was added. The mixture was stirred overnight at RT. Scavenger was filtered off and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and then re-purified by RP-FCC (C18HP; H₂O:MeCN) to give a product (0.086 g, 39% yield) as a yellow foam. ESI-MS: 598.7 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.7 mL, 1.0 eq. to FB) and DCM as a solvent (5.0 mL) to give the product (0.09 g, 98% yield) as a yellow solid. HR-MS: 598.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.36 (d, J=5.8 Hz, 1H), 8.26 (d, J=1.5 Hz, 1H), 8.09 (dd, J=2.7, 1.5 Hz, 1H), 7.95 (s, 1H), 7.77 (d, J=2.7 Hz, 1H), 7.75-7.61 (m, 3H), 6.95 (d, J=7.6 Hz, 1H), 4.77 (d, J=12.4 Hz, 1H), 4.38-4.01 (m, 5H), 3.77-3.56 (m, 5H), 3.50-3.40 (m, 2H), 3.29-3.12 (m, 2H), 3.03 (t, J=12.6 Hz, 1H), 2.60-2.48 (m, 4H), 2.26 (d, J=11.5 Hz, 1H), 2.21-2.11 (m, 1H), 2.04-1.79 (m, 3H), 1.65 (q, J=12.8 Hz, 1H), 1.37-1.27 (m, 2H), 1.07-0.97 (m, 2H).

Example 92. 1-cyclopropyl-3-({[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl]carbamate

A solution of (S)-3-Boc-aminopiperidine (0.55 g, 1.1 eq.), 5-bromo-N,N-dimethylpyridin-2-amine (0.50 g, 1.0 eq.) and sodium tert-butoxide (0.30 g, 1.3 eq.) in 1,4-dioxane was purged with argon for 10 min. Subsequently, RuPhos Pd G3 (0.21 g, 0.1 eq.) was added and the reaction mixture was stirred for 24 h at 100° C. The reaction mixture was filtered through a pad of celite and concentrated to dryness under reduced pressure. The residue was diluted with DCM and water. The separated organic layer was dried over anh. Na₂SO₄, filtered and concentrated. The crude material was purified by FCC (SiHP, DCM:EtOAc) give product (0.17 g, 21% yield) as a yellow oil. ESI-MS: 321.4 [M+H]⁺.

Preparation of 5-[(3S)-3-aminopiperidin-1-yl]-N,N-dimethylpyridin-2-amine trifluoroacetate

Trifluoroacetic acid (0.4 mL, 10.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl]carbamate (0.17 g, 1.0 eq.) in DCM (10.0 mL) and the mixture was stirred for 3 h at RT. After that time the mixture was concentrated in vacuo and traces of moisture were azeotropically removed with MeOH to give a product (0.17 g, 94% yield) as a yellow oil. ESI-MS: 221.2 [M+H]⁺. The product was used in next step without further purification.

Preparation of N,N-dimethyl-5-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-amine

2-methylpyridine-4-carbaldehyde (0.054 mL, 1.0 eq.), 5-[(3S)-3-aminopiperidin-1-yl]-N,N-dimethylpyridin-2-amine trifluoroacetate (0.17 g, 0. 1.0 eq.) and sodium acetate (0.04 g, 1.0 eq.) w dissolved in anh. methanol (5.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.037 g, 2.0 eq.) was added. The reaction was stirred for 3 h at RT. The reaction mixture was then concentrated in vacuo and residue was partitioned between DCM and aqueous NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The product mixture was purified by FCC (SiHP; DCM:MeOH) to give product (0.16 g, 82% yield) as a yellow oil. ESI-MS: 326.3 [M+H]⁺.

Preparation of 1-cyclopropyl-3-({[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1,4-dihydroquinolin-4-one

A solution of N,N-dimethyl-5-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-amine (0.13 g, 1.0 eq.) and 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.10 g, 1.1 eq.) in anh. DCE (5.0 mL) was stirred at 60° C. for 3 h, then reaction was cooled to 0° C., sodium triacetoxyborohydride (0.42 g, 5.0 eq.) was added and the reaction mixture was stirred overnight at 60° C. After that time reaction mixture was concentrated and the crude material was diluted with DCM and washed with water and brine. The organic layer was combined, dried over Na₂SO₄, filtered and concentrated. Crude was purified on FCC (SiHP; DCM:MeOH) to give product (0.16 g, 41% yield) as a yellow oil. ESI-MS: 559.5 [M+H]⁺.

Preparation of 1-cyclopropyl-3-({[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Sodium hydride (60% dispersion in mineral oil, 0.034 g, 5.0 eq.) was added in portions to a solution of 1-cyclopropyl-3-({[(3S)-1-[6-(dimethylamino)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1,4-dihydroquinolin-4-one (0.16 g, 1.0 eq.) in ethylene glycol (2.0 mL). The reaction mixture was stirred at 60° C. overnight. The reaction mixture was quenched with ice-cold water and extracted with DCM (3×). The combined organic layers were washed with water, brine and dried with anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) to give product (0.11 g, 63% yield) as a yellow solid. ESI-MS: 601.6 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.09 mL, 1.0 eq. to FB) and DCM as a solvent (5.0 mL) to give the product (0.12 g, 99% yield) as a green-yellow solid. HR-MS: 601.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.21 (d, J=5.4 Hz, 1H), 7.95 (s, 1H), 7.87 (dd, J=15.9, 10.3 Hz, 2H), 7.58 (d, J=7.1 Hz, 1H), 7.44-7.31 (m, 3H), 7.05 (d, J=9.7 Hz, 1H), 4.37-4.25 (m, 2H), 4.09-3.66 (m, 7H), 3.54-3.45 (m, 1H), 3.44-3.36 (m, 1H), 3.19 (s, 6H), 3.15-2.98 (m, 1H), 2.86 (s, 1H), 2.69 (s, 1H), 2.39 (s, 3H), 2.15 (d, J=6.9 Hz, 1H), 2.04-1.92 (m, 1H), 1.80-1.67 (m, 2H), 1.36-1.30 (m, 2H), 1.01-0.93 (m, 2H).

Example 93. 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]carbamate

A suspension of 3-Bromo-5-(trifluoromethyl)pyridine (1.5 g, 1.0 eq.), (S)-3-Boc-aminopiperidine (1.7 g, 1.3 eq.) and Cs₂CO₃ (2.9 g, 1.4 eq.) in anh. 1,4-dioxane (20.0 mL) was sparged with argon over 20 min and after this Pd₂(dba)₃ (0.30 g, 0.05 eq.) and Xantphos (0.38 g, 0.1 eq.) were added. The reaction tube was closed and reaction was stirred at 140° C. for 2 days. The mixture was filtered through celite pad and concentrated in vacuo. Product was purified on FCC (SiHP; Hex:EtOAc), dissolved in EtOAc and scavenger QuadraSil MP (2.7 g) was added. The mixture was stirred overnight at RT. Scavenger was filtered off and washed with EtOAc. The solvent was concentrated to give the product (1.9 g, 82% yield) as a yellow solid. ESI-MS: 346.1 [M+H]⁺.

Preparation of (3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-amine Trifluoroacetate

Trifluoroacetic acid (4.1 mL, 10.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]carbamate (1.9 g, 1.0 eq.) in DCM (35.0 mL) at room temperature and the mixture was stirred for 3 days at RT. After that time the mixture was concentrated in vacuo and traces of moisture were removed by azeotropic distillation with MeOH to give a product (1.7 g, 99% yield) as an orange solid. The product was used in next step without further purification. ESI-MS: 246.2 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-amine

(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-amine trifluoroacetate (1.7 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.60 mL, 1.0 eq.) and sodium acetate (0.44 g, 1.0 eq.) were dissolved in anh. methanol (20.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.41 g, 2.0 eq.) was added. The reaction was stirred overnight at RT. After that time the reaction was concentrated in vacuo and residue was extracted with DCM and aqueous NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated under reduced pressure.

The product mixture was purified by FCC (SiHP; DCM:MeOH) to give product (1.63 g, 79% yield) as a pale yellow solid. ESI-MS: 351.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.65 g, 1.0 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-amine (0.98 g, 1.0 eq.) in anh. DCE (25.0 mL) was stirred for 5 h at 50° C. Then the mixture was cooled to RT and sodium triacetoxyborohydride (2.7 g, 5.0 eq.) was added. The resulting mixture was stirred overnight at 60° C. After that time the mixture was diluted with DCM and washed with 0.1 M NaOH aq. solution. The aqueous layer was washed with DCM. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. The product was purified by FCC (SiHP; DCM:MeOH) to give product (1.05 g, 58% yield) as a white foam. ESI-MS: 584.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Sodium hydride (60% dispersion in mineral oil, 0.3 g, 5.0 eq.) was added in portions to a solution of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-[5-(trifluoromethyl)pyridin-3-yl]piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (1.05 g, 1.0 eq.) in ethylene glycol (15.0 mL) at 0° C. The mixture was stirred at 0° C. for 1 h and warmed to RT. Then the mixture was stirred overnight at 60° C. After that time the reaction was quenched by water addition. The mixture was diluted with water and washed with DCM. The organic layer was dried over Na₂SO₄. The product was purified by FCC (SiHP; DCM:MeOH) and re-purified by FCC using HPLC grade solvents (SiHP; DCM:MeOH) to give product (0.68 g, 72% yield) as a white foam. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.5 mL, 1.0 eq. to FB) and DCM as a solvent (12.0 mL) to give the product (0.63 g, 89% yield) as a pale yellow solid. HRMS: 626.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.53 (d, J=2.8 Hz, 1H), 8.37 (d, J=6.0 Hz, 1H), 8.22 (s, 1H), 8.07 (s, 1H), 7.90 (d, J=11.4 Hz, 1H), 7.82 (s, 1H), 7.76 (d, J=5.5 Hz, 1H), 7.65-7.58 (m, 2H), 4.37-4.30 (m, 2H), 4.30-4.11 (m, 3H), 4.11-3.88 (m, 4H), 3.86-3.78 (m, 1H), 3.60-3.52 (m, 1H), 3.25-3.06 (m, 2H), 2.92 (td, J=12.3, 2.3 Hz, 1H), 2.57 (s, 3H), 2.31-2.19 (m, 1H), 2.06-1.95 (m, 1H), 1.91-1.66 (m, 2H), 1.43-1.32 (m, 2H), 1.11-0.97 (m, 2H).

Example 94. 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

The solution of 7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 27) (0.80 g, 1.0 eq.) and (S)-Pyrrolidin-3-ylmethanol (0.27 g, 1.8 eq.) in anh. 1,4-dioxane (15.0 mL) was purged with argon for 15 min. Then sodium tert-butoxide (0.29 g, 2.1 eq.), BINAP (0.27 g, 0.3 eq.) and Pd₂(dba)₃ (0.13 g, 0.1 eq.) were added. The resulting mixture was heated overnight at 95° C. The reaction mixture was filtered through a pad of celite and concentrated under reduced pressure. The residue was dissolved in DCM and scavenger QuadraSil MP (4.0 eq.) was added. The mixture was stirred overnight at RT. Scavenger was filtrated off and the filtrate was concentrated in vacuo. The residue was purified by FCC (SiHP; DCM:MeOH) and then re-purified by RP-FCC (C18HP; H2O:MeCN) to give a product (0.43 g, 48% yield) as a yellow foam. ESI-MS: 598.5 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.35 mL, 1.0 eq. to FB) and DCM as a solvent (32.0 mL) to give the product (0.43 g, 94% yield) as a yellow solid. HRMS: 598.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.37 (d, J=6.0 Hz, 1H), 8.28 (d, J=1.5 Hz, 1H), 8.10 (dd, J=2.7, 1.5 Hz, 1H), 7.97 (s, 1H), 7.79 (d, J=2.7 Hz, 1H), 7.77-7.70 (m, 2H), 7.70-7.64 (m, 1H), 6.96 (d, J=7.6 Hz, 1H), 4.79 (d, J=12.3 Hz, 1H), 4.33 (s, 2H), 4.30-4.21 (m, 1H), 4.13 (s, 2H), 3.79-3.57 (m, 5H), 3.52-3.42 (m, 2H), 3.31-3.12 (m, 2H), 3.11-3.00 (m, 1H), 2.62-2.54 (m, 1H), 2.53 (s, 3H), 2.32-2.24 (m, 1H), 2.24-2.12 (m, 1H), 2.05-1.92 (m, 2H), 1.87 (s, 1H), 1.73-1.60 (m, 1H), 1.34 (dd, J=6.9, 1.7 Hz, 2H), 1.03 (dq, J=7.5, 3.8 Hz, 2H).

Example 95. 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of (3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-amine

2-Chloropyrazine (0.9 g, 1.0 eq.) was added to the mixture of KHCO₃ (1.6 g, 2.0 eq.), tert-butyl N-[(3S,5S)-5-fluoropiperidin-3-yl]carbamate (1.9 g, 1.1 eq.) in DMSO (10.0 mL) under inert atmosphere. The reaction mixture was stirred at 100° C. for 16 h. The reaction was cooled to RT and water was added (50.0 mL). The reaction mixture was washed with diethyl ether (3×20.0 mL). The combined organic layers were dried over Na₂SO₄ filtered and the volatiles were evaporated to furnish an orange oil (2.2 g). ESI-MS: 297.4 [M+H]⁺. The orange oil was then dissolved in 1,4-dioxane (20.0 mL). The pH of the mixture was adjusted to between pH=0.5-1.0. The solution was then heated at 65° C. for 1.5 h. The reaction was cooled to RT and the volatiles were evaporated. The crude mixture was then dissolved in water (20.0 mL) and basified using NaOH (solid) until pH>12. The organics were extracted using EtOAc (3×20.0 mL), the organic layer dried over Na₂SO₄, filtered and the volatiles were evaporated to obtain the product (0.78 g, 51% yield) as a yellow oil. ESI-MS: 197.1 [M+H]⁺.

Preparation of (3S,5S)-5-fluoro-N-[(2-methylpyridin-4-yl)methyl]-1-(pyrazin-2-yl)piperidin-3-amine

A mixture of (3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-amine (0.78 g, 1.0 eq.), 2-methylpyridine-4-carboxaldehyde (0.45 mL, 1.1 eq.), sodium acetate (0.33 g, 1.0 mL) and anh. methanol (20.0 mL) was stirred overnight at RT under N₂. Sodium borohydride (0.20 g, 1.3 eq.) was added to the mixture in portions over 10 min. The mixture was then stirred at RT for 1 h. Then MeOH was evaporated and the crude material was dissolved in DCM (50.0 mL) and washed with water (2×20.0 mL). Organic layers were combined, dried over Na₂SO₄, filtered and concentrated. Crude product was purified by RP-FCC (C18HP; H₂O:MeCN) to give desired product (0.66 g, 55% yield) as a light yellow oil. ESI-MS: 302.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(0.72 g, 1.3 eq.) and (3S,5S)-5-fluoro-N-[(2-methylpyridin-4-yl)methyl]-1-(pyrazin-2-yl)piperidin-3-amine (0.66 g, 1.0 eq.) in anh. DCE (22.0 mL) was heated at 60° C. for 4 h. Then the mixture was cooled to 0° C. and sodium triacetoxyborohydride (1.8 g, 4.0 eq.) was added in portions. The resulting mixture was stirred 2 days at RT. 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.36 g, 0.65 eq.) and sodium triacetoxyborohydride (0.92 g, 2.0 eq.) were added and reaction mixture was stirred overnight at RT. The reaction mixture was quenched with water and washed with DCM. Organic layer was washed with brine, dried over Na₂SO₄ and concentrated in vacuo. Crude product was purified by FCC (SiHP, DCM:MeOH), re-purified by FCC (SiHP, EtOAc:MeOH) to give product (0.85 g, 71% yield) as a white solid. ESI-MS: 535.4 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

1-Cyclopropyl-6,7-difluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one (0.85 g, 1.0 eq.) was dissolved in ethylene glycol (15.0 mL) and the mixture was cooled to 0° C. Then sodium hydride (60% dispersion in mineral oil, 0.31 g, 5.0 eq.) was added in portions. The resulting mixture was stirred at 60° C. overnight. The reaction mixture was diluted with water and extracted to DCM. Organic layers were washed with brine, dried over Na₂SO₄ and concentrated in vacuo. Crude product was purified by FCC (SiHP; DCM:MeOH) and re-purified by FCC (SiHP; DCM:MeOH) using HPLC grade solvents to give product (0.89 g, 81% yield). ESI-MS: 577.4 [M+H]⁺. The compound (0.7 g) was converted to the HCl salt using 2 M HCl in Et₂O (0.6 mL, 1.0 eq. to FB) and DCM as a solvent (10.0 mL) to give the product (0.69 g, 90% yield) as a yellow solid. HR-MS: 577.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.45 (d, J=6.1 Hz, 1H), 8.26 (d, J=1.3 Hz, 1H), 8.09 (s, 1H), 8.06 (dd, J=2.7, 1.5 Hz, 1H), 7.97 (s, 1H), 7.93-7.86 (m, 2H), 7.75 (d, J=2.7 Hz, 1H), 7.62 (d, J=7.1 Hz, 1H), 5.09 (d, J=48.4 Hz, 1H), 4.81 (d, J=12.6 Hz, 1H), 4.59 (t, J=13.6 Hz, 1H), 4.36-4.30 (m, 2H), 4.18 (s, 2H), 4.04-3.98 (m, 2H), 3.91-3.80 (m, 2H), 3.56 (tt, J=7.2, 4.0 Hz, 1H), 3.31-3.08 (m, 3H), 2.67 (s, 3H), 2.52-2.40 (m, 1H), 2.18-1.99 (m, 1H), 1.40-1.30 (m, 2H), 1.10-0.93 (m, 2H).

Example 96. 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-(pyridazin-3-yl)piperidin-3-yl]carbamate

A solution of 3-bromopyridazine (1.0 g, 1.0 eq.) and (S)-3-Boc-aminopiperidine (5.0 g, 4.0 eq.) in EtOH (63.0 mL) was heated overnight at 90° C. Solvent was evaporated and the residue was dissolved in DMC and washed with water and brine. The separated organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. Crude product was purified by FCC (SiHP; DCM:MeOH) to give product (1.2 g, 66% yield) as an orange oil. ESI-MS: 279.2 [M+H]⁺.

Preparation of (3S)-1-(pyridazin-3-yl)piperidin-3-amine Hydrochloride

tert-Butyl N-[(3S)-1-(pyridazin-3-yl)piperidin-3-yl]carbamate (1.33 g, 1.0 eq.) was dissolved in DCM (8.0 mL) and 4 M hydrogen chloride in 1,4-dioxane (5.9 mL, 5.0 eq.) was added. The reaction mixture was stirred overnight at RT. Solvents were evaporated. The residue was co-concentrated with DCM several times to give product (1.1 g, quantitative yield) as an orange solid. ESI-MS: 179.1 [M+H]⁺.

Preparation of (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridazin-3-yl)piperidin-3-amine

A solution of (3S)-1-(pyridazin-3-yl)piperidin-3-amine hydrochloride (1.1 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.70 mL, 1.3 eq.) and sodium acetate (0.61 g, 1.5 eq.) in anh. methanol (16.0 mL) was stirred for 3 days at RT. After that time, the mixture was cooled to 0° C. and sodium borohydride (0.37 g, 2.0 eq.) was added. The reaction was stirred at RT for 1.5 h. Solvent was evaporated. The residue was dissolved in DMC and washed with 1 M NaOH and subsequently with brine. Separated organic layer was dried over anh. Na₂SO₄, filtered and concentrated in vacuo. Crude product was purified by FCC (SiHP; DCM:MeOH) to give product (0.97 g, 69% yield) as a yellow oil. ESI-MS: 284.1 [M+H]⁺.

Preparation of 1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4)(1.12 g, 1.3 eq.) and (3S)—N-[(2-methylpyridin-4-yl)methyl]-1-(pyridazin-3-yl)piperidin-3-amine (0.97 g, 1.0 eq.) in anh. DCE (34.0 mL) was heated overnight at 50° C. Then the mixture was cooled to 0° C. and sodium triacetoxyborohydride (2.9 g, 4.0 eq.) was added. The resulting mixture was stirred for 1.5 h at RT. The reaction mixture was quenched with water and washed with DCM. Separated organic layer was washed with brine, dried over Na₂SO₄ and concentrated in vacuo. The crude product was purified by FCC (SiHP, DCM:MeOH) to give product (0.72 g, 40% yield) as a yellow solid. ESI-MS: 517.3 [M+H]⁺.

Preparation of 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one

1-cyclopropyl-6,7-difluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridazin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (0.72 g, 1.0 eq.) was dissolved in ethylene glycol (14.0 mL) and mixture was cooled to 0° C. Then sodium hydride (60% dispersion in mineral oil, 0.27 g, 5.0 eq.) was added in portions. The resulting mixture was stirred at 60° C. overnight. The reaction mixture was diluted with water and extracted to DCM. Organic layers were washed with brine, dried over Na₂SO₄ and concentrated in vacuo. The crude product was purified by RP-FCC (C18HP; H₂O:MeCN) and re-purified twice by FCC (SiHP; DCM:MeOH to give product (0.13 g, 15% yield) as a white solid. ESI-MS: 559.5 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.43 (dd, J=4.4, 1.2 Hz, 1H), 8.17 (d, J=5.2 Hz, 1H), 7.98 (s, 1H), 7.91 (d, J=11.6 Hz, 1H), 7.57 (d, J=7.2 Hz, 1H), 7.39-7.35 (m, 1H), 7.31-7.28 (m, 1H), 7.28-7.26 (m, 1H), 7.25-7.21 (m, 1H), 4.74-4.68 (m, 1H), 4.33-4.26 (m, 3H), 4.02-3.98 (m, 2H), 3.90-3.86 (m, 2H), 3.84-3.78 (m, 2H), 3.53-3.47 (m, 1H), 3.14-3.06 (m, 1H), 2.99-2.91 (m, 1H), 2.90-2.82 (m, 1H), 2.36 (s, 3H), 2.22-2.14 (m, 1H), 1.96-1.89 (m, 1H), 1.87-1.76 (m, 1H), 1.66-1.54 (m, 1H), 1.36-1.29 (m, 2H), 0.99-0.93 (m, 2H).

Example 97. 3-({[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of tert-butyl N-[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl]carbamate

A solution of (S)-3-Boc-aminopiperidine (1.04 g, 1.0 eq.), 5-bromo-2-chloropyridine (1.0 g, 1.0 eq.) and Cs₂CO₃ (3.4 g, 2.0 eq.) in 1,4-dioxane (20.0 mL) was purged with argon for 10 min. Then Xantphos (0.3 g, 0.10 eq.) and Pd₂(dba)₃ (0.24 g, 0.05 eq.) were added and the reaction mixture was stirred overnight at 120° C. Two identical reactions starting with 0.1 g of 5-bromo-2-chloropyridine were also carried out at the same time. The crude reaction mixtures of all these batches were combined, diluted with DCM and washed with water. Aqueous layer was washed with DCM and the organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The mixture obtained was purified by FCC (SiHP; Hex:EtOAc). Product was dissolved in EtOAc and 4.0 eq of scavenger QuadraSil MP were added. The mixture was stirred overnight at RT. After that time the mixture was filtered. Filtrate was concentrated in vacuo to give product (1.09 g, 56% yield) as a yellow solid. ESI-MS: 312.1 [M+H]⁺.

Preparation of (3S)-1-(6-chloropyridin-3-yl)piperidin-3-amine Trifluoroacetate

Trifluoroacetic acid (2.6 mL, 10.0 eq.) was added to a solution od tert-butyl N-[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl]carbamate (1.09 g, 1.0 eq.) in DCM (15.0 mL). The mixture was stirred overnight at RT. After that time the mixture was concentrated in vacuo after dilution with DCM. The product (1.1 g, 89% yield) was used in next step without further purification. ESI-MS: 212.1 [M+H]⁺.

Preparation of (3S)-1-(6-chloropyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

(3S)-1-(6-chloropyridin-3-yl)piperidin-3-aminetrifluoroacetate (1.1 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.47 mL, 1.4 eq.) and sodium acetate (0.25 g, 1.0 eq.) were dissolved in anh. MeOH (15.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.23 g, 2.0 eq.) was added. The reaction was stirred at RT for 2 h. After that time the reaction was concentrated in vacuo and residue was partitioned between DCM and water. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated. The product mixture was purified by FCC (SiHP; DCM:MeOH) to give a product (0.82 g, 81% yield) as a yellow oil. ESI-MS: 317.9 [M+2]+.

Preparation of 1-cyclopropyl-6-fluoro-7-[2-(oxan-2-yloxy)ethoxy]-4-oxo-1,4-dihydroquinoline-3-carbaldehyde

7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (1.0 g, 1.0 eq.), dipotassium carbonate (1.02 g, 2.0 eq.), tBuBrettPhos (0.045 g, 0.025 eq.) and Pd(dba)₂ (0.027 g, 0.013 eq.) were dissolved in 2-methyltetrahydrofuran (10.0 mL) under nitrogen atmosphere. Then, 2-(Tetrahydro-2H-pyran-2-yloxy)ethanol (0.60 mL, 1.2 eq.) was added. The reaction mixture was stirred at 95° C. for 16 h. After completion of the reaction, 10 mL of water was added to the reaction mixture. It was stirred for 15 min and 10 mL of 2-MeTHF was added. Then, the biphasic mixture was poured into a separatory funnel and organic phase was collected. The aqueous phase was washed with 2-MeTHF (3×10 mL). Combined organic layers were dried over sodium sulphate, filtered and concentrated to half of its volume. 20 mL of n-heptane was added and the 2-MeTHF was evaporated under reduced pressure. During evaporation, yellow solids precipitated. Solid was filtered and dried on vacuum to give a product (0.49 g, 33% yield). ESI-MS: 376.5 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6-fluoro-7-[2-(oxan-2-yloxy)ethoxy]-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-6-fluoro-7-[2-(oxan-2-yloxy)ethoxy]-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (0.09 g, 1.0 eq.) and (3S)-1-(6-chloropyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.097 g, 1.4 eq.) in anh. DCE (2.0 mL) was stirred for 1 h at RT. Then STAB (0.221 g, 5.0 eq.) was added and the mixture was stirred over weekend at RT. After that time the mixture was diluted with DCM and washed with water. Aqueous layer was extracted with DCM. The organic layer was washed with brine, dried over Na₂SO₄, filtered and evaporated. The product was purified by FCC (SiHP; DCM:MeOH) to give product (0.124 g, 70% yield). ESI-MS: 676.7 [M+H]⁺.

Preparation of 3-({[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one Hydrochloride

Trifluoroacetic acid (0.055 mL, 5.0 eq.) was added to a solution of 3-({[(3S)-1-(6-chloropyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6-fluoro-7-[2-(oxan-2-yloxy)ethoxy]-1,4-dihydroquinolin-4-one (0.124 g, 1.0 eq.) in DCM (1.5 mL) and the mixture was stirred overnight at RT. After that time the mixture was concentrated in vacuo. The residue was dissolved in water, basified with saturated NaHCO₃, and extracted with DCM. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated in vacuo. The product mixture was purified by FCC (SiHP; DCM:MeOH) and the residue was dissolved in MeOH, scavenger QuadraSip MP was added and the mixture was stirred for 48 h at RT. After that time the mixture was filtered off and filtrate was concentrated in vacuo. The residue was re-purified by FCC (SiHP; DCM:MeOH) to give product (0.065 g, yield 72%) as a colorless oil. ESI-MS: 592.6 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.05 mL, 1.0 eq. to FB) and DCM as a solvent (1.0 mL) to give the product (0.056 g, 85% yield) as a white solid. ¹H NMR (400 MHz, Methanol-d₄) δ 8.39 (d, J=6.0 Hz, 1H), 8.08 (s, 1H), 8.03 (d, J=3.1 Hz, 1H), 7.90 (d, J=11.4 Hz, 1H), 7.81 (br s, 1H), 7.76 (br d, 1H), 7.63 (d, J=7.1 Hz, 1H), 7.46 (dd, J=8.9, 3.2 Hz, 1H), 7.25 (d, J=8.9 Hz, 1H), 4.37-4.30 (m, 2H), 4.30-4.19 (m, 2H), 4.12-3.91 (m, 5H), 3.69-3.62 (m, 1H), 3.60-3.52 (m, 1H), 3.27-3.17 (m, 1H), 3.14-3.03 (m, 1H), 2.91-2.81 (m, 1H), 2.57 (s, 3H), 2.26-2.19 (m, 1H), 2.04-1.95 (m, 1H), 1.88-1.66 (m, 2H), 1.41-1.33 (m, 2H), 1.11-1.00 (m, 2H).

Example 98. 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

Preparation of 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one Hydrochloride

7-chloro-1-cyclopropyl-6-fluoro-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 27) (0.8 g, 1.0 eq.), (3S)-Piperidin-3-ol, HCl (0.36 g, 1.8 eq.) and sodium tert-butoxide (0.29 g, 2.1 eq.) were dissolved in anh. 1,4-dioxane (15.0 mL) and purged with argon for 15 min. Then BINAP (0.27 g, 0.3 eq.) and Pd₂(dba)₃ (0.13 g, 0.1 eq.) were added and reaction was stirred overnight at 95° C. Reaction mixture was cooled to RT and filtered through a pad of celite. The filtrate was concentrated and then residue was dissolved in DCM and washed with water. Organic layers were collected, dried over anh. Na₂SO₄, filtered and evaporated. Product was purified by FCC (SHiP, DCM:MeOH). The Residue was dissolved in anh. DCM and 4.0 eq. of Scavenger QuadraSip MP was added. The mixture was stirred overnight at RT. After that time the mixture was filtered off. Filtrate was concentrated under reduced pressure and the product was re-purified by FCC (SHiP, DCM:MeOH) to obtain the product (0.58 g, 66% yield) as a beige solid. ESI-MS: 598.5 [M+H]⁺. The compound was converted to the HCl salt using 2 M HCl in Et₂O (0.5 mL, 1.0 eq. to FB) and DCM as a solvent (43.0 mL) to give the product (0.59 g, yield 97%) as a yellow solid. HR-MS: 598.3 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.40 (d, J=5.9 Hz, 1H), 8.26 (d, J=1.5 Hz, 1H), 8.11-8.07 (m, 1H), 8.05 (s, 1H), 7.86-7.69 (m, 4H), 7.45 (d, J=7.4 Hz, 1H), 4.76 (d, J=12.4 Hz, 1H), 4.38-4.20 (m, 3H), 4.15-3.96 (m, 2H), 3.93-3.82 (m, 1H), 3.68 (dd, J=11.7, 3.9 Hz, 1H), 3.59-3.44 (m, 2H), 3.29-3.06 (m, 2H), 3.06-2.94 (m, 2H), 2.86 (dd, J=11.7, 8.6 Hz, 1H), 2.56 (s, 3H), 2.31-2.19 (m, 1H), 2.13-2.02 (m, 1H), 2.02-1.86 (m, 3H), 1.83-1.69 (m, 1H), 1.69-1.58 (m, 1H), 1.58-1.46 (m, 1H), 1.43-1.27 (m, 2H), 1.11-0.98 (m, 2H).

Example 99. N-{5-[(3S)-3-{[(1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methoxypyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}acetamide

Preparation of N-{5-[(3S)-3-{[(1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methoxypyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}acetamide

TEA (0.21 mL, 6.0 eq.) was added to a mixture of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one hydrochloride (Example 4) (0.15 g, 1.0 eq.) in DMF (1.0 mL) placed in an ice bath and was followed by dropwise addition of Ac₂O (0.029 mL, 1.2 eq.). The resulting mixture was stirred overnight at RT. The reaction mixture was concentrated in vacuo and then concentrated again after dilution with n-heptane. The residue was purified by FCC (SiHP; DCM:MeOH) to give the product (0.12 g, 76% yield). ESI-MS: 589.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 10.19 (s, 1H), 8.04-7.94 (m, 4H), 7.92 (s, 1H), 7.88 (d, J=9.0 Hz, 1H), 7.35 (dd, J=9.1, 3.1 Hz, 1H), 6.95 (dd, J=5.2, 1.3 Hz, 1H), 6.77 (s, 1H), 3.84-3.69 (m, 6H), 3.69-3.57 (m, 2H), 3.59-3.52 (m, 1H), 3.53-3.44 (m, 1H), 2.81-2.69 (m, 2H), 2.57 (td, J=12.6, 3.3 Hz, 1H), 2.03 (s, 3H), 1.98-1.88 (m, 1H), 1.84-1.69 (m, 1H), 1.59-1.42 (m, 2H), 1.29-1.14 (m, 2H), 0.97-0.81 (m, 2H).

Example 100. 1-cyclopropyl-3-({[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one

Preparation of tert-butyl N-[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl]carbamate

(S)-3-Boc-aminopiperidine (0.46 g, 1.5 eq.) was added to the mixture of 5-Bromo-2-cyclo-propylpyridine (0.3 g, 1.0 eq.) and cesium carbonate (0.67 g, 1.4 eq.) in anh. 1,4-dioxane (15.0 mL). The mixture was purged with argon for 10 min. Subsequently, Xantphos (0.053 g, 0.06 eq.) and Pd₂(dba)₃ (0.07 g, 0.05 eq.) were added, vessel was closed and the reaction mixture was stirred at 110° C. over the weekend. After that time, the reaction mixture was filtered through a pad of celite and concentrated under reduced pressure. The residue was diluted with DCM and washed with water, brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. Product was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.14 g, 29% yield) as a clear oil. ESI-MS: 318.5 [M+H]⁺.

Preparation of (3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-amine

Trifluoroacetic acid (0.33 mL, 10.0 eq.) was added to a solution of tert-butyl N-[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl]carbamate (0.14 g, 1.0 eq.) in DCM (5.0 mL) and the reaction was stirred for 3 h at RT. After that time, the mixture was concentrated in vacuo. The residue was diluted with DCM and washed with 5 M NaOH, brine, dried over anh. sodium sulfate and concentrated under reduced pressure. The crude product (0.093 g, 97% yield) was used in next step without any purification. ESI-MS: 218.0 [M+H]⁺.

Preparation of (3S)-1-(6-cyclopropylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine

(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-amine (0.16 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.08 mL, 1.0 eq.) and sodium acetate (0.06 g, 1.0 eq.) were dissolved in anh. methanol (3.0 mL) and the mixture was stirred overnight at RT. After that time the mixture was cooled to 0° C. and sodium borohydride (0.054 g, 2.0 eq.) was added. The reaction was stirred overnight at RT. The reaction mixture was then concentrated in vacuo and residue was extracted with DCM and aqueous NaOH solution (pH was adjusted to 11). The organic layer was washed with brine, dried over Na₂SO₄, filtered and evaporated. Product was purified by RP-FCC (C18HP; H₂O:MeCN) to give product (0.17 g, 72% yield) as a yellow oil. ESI-MS: 323.4 [M+H]⁺.

Preparation of 1-cyclopropyl-3-({[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1,4-dihydroquinolin-4-one

A solution of 1-cyclopropyl-6,7-difluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 4) (0.13 g, 1.0 eq.) and (3S)-1-(6-cyclopropylpyridin-3-yl)-N-[(2-methylpyridin-4-yl)methyl]piperidin-3-amine (0.16 g, 1.0 eq.) was stirred in anh. DCE (6.0 mL) at 60° C. for 1 h. Then the solution was cooled to RT and STAB (0.54 g, 5.0 eq.) was added. The heating at 60° C. was continued overnight. The reaction mixture was diluted with DCM, washed with water, brine, dried over anh. sodium sulfate, filtered and evaporated. The residue was purified by FCC (SiHP; DCM:MeOH) give product (0.13 g, 44% yield) as a clear oil. ESI-MS: 556.5 [M+H]⁺.

Preparation of 1-cyclopropyl-3-({[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6-fluoro-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one

A mixture of 1-cyclopropyl-3-({[(3S)-1-(6-cyclopropylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1,4-dihydroquinolin-4-one (0.13 g, 1.0 eq.), sodium hydride (60% dispersion in mineral oil, 0.027 g, 5.0 eq.) and ethylene glycol (2.0 mL) was stirred at 60° C. overnight. The reaction mixture was cooled to RT, diluted with water, basified using 5 M NaOH and extracted to DCM. The organic layer was washed with brine, dried over anh. Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by FCC (SiHP; DCM: MeOH) give the product (0.08 g, 59% yield) as white solid. ESI-MS: 598.6 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J=5.0 Hz, 1H), 8.09 (d, J=2.8 Hz, 1H), 7.84 (s, 1H), 7.79 (d, J=11.7 Hz, 1H), 7.51 (d, J=7.2 Hz, 1H), 7.23-7.14 (m, 3H), 7.05 (d, J=8.5 Hz, 1H), 5.03 (t, J=5.4 Hz, 1H), 4.24 (t, J=4.8 Hz, 2H), 3.85-3.69 (m, 5H), 3.65-3.47 (m, 4H), 2.79-2.69 (m, 2H), 2.62-2.54 (m, 1H), 2.37 (s, 3H), 2.00-1.90 (m, 2H), 1.79-1.71 (m, 1H), 1.58-1.41 (m, 2H), 1.28-1.18 (m, 2H), 0.94-0.71 (m, 6H).

Example 101. 3-({[(3S)-1-[6-(2-hydroxyethoxy)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

Preparation of 2-[(5-bromopyridin-2-yl)oxy]ethan-1-ol

A suspension of 2,5-dibromopyridine (1.0 g, 1.0 eq.), ethane-1,2-diol (1.2 mL, 5.0 eq.) and NaH (60% dispersion in mineral oil, 0.20 g, 1.2 eq.) was stirred in anh. DMF (12.0 mL) for 1 h at RT, then reaction mixture was stirred overnight at 60° C. Then solvent was evaporated in vacuo and crude product was purified by FCC (SiHP; Hex:EtOAc) to give the product (0.68 g, 74% yield) as a colorless oil. ESI-MS: 219.9 [M+2]+.

Preparation of (5-bromopyridin-2-yl)methyl Acetate

Ac₂O (0.35 mL, 1.2 eq.) was added to a solution of 2-[(5-bromopyridin-2-yl)oxy]ethan-1-ol (0.68 g, 1.0 eq.) and TEA (1.7 mL, 4.0 eq.) in anh. DCM (15.0 mL). The reaction mixture was stirred for 3 h at RT, then crude mixture was concentrated in vacuo and purified by FCC (SiHP; Hex:EtOAc) to give the product (0.65 g, 76% yield) as a colorless oil. ESI-MS: 262.1 [M+2H]⁺.

Preparation of 2-({5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}oxy)ethyl acetate

A suspension of 2-[(5-bromopyridin-2-yl)oxy]ethyl acetate (0.2 g, 1.0 eq.), 1-methyl-3-({[(2-methylpyridin-4-yl)methyl][(3S)-piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one (Intermediate 5) (0.29 g, 1.1 eq.) and Cs₂CO₃ (0.48 g, 2.0 eq.) in 1,4-dioxane (5.0 mL) was purged with argon for 10 min. Then Pd₂(dba)₃ (0.033 g, 0.05 eq.) and Xantphos (0.018 g, 0.06 eq.) were added and the reaction mixture was purged with argon for a few minutes. The reaction mixture was stirred at 120° C. for 24 h and then filtered through a pad of celite, washed with DCM and concentrated in vacuo. The residue was stirred for 4 h with scavenger QuadraPure MPA in DCM, filtered, evaporated in vacuo and purified by FCC (SiHP; DCM:MeOH) to give the product (0.138 g, 33% yield) as a yellow solid. ESI-MS: 556.7 [M+H]⁺.

Preparation of 3-({[(3S)-1-[6-(2-hydroxyethoxy)pyridin-3-yl]piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one

2-({5-[(3S)-3-{[(1-methyl-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridin-2-yl}oxy)ethyl acetate (0.138 g, 1.0 eq.) was suspended in water (1.0 mL) and methanol (5.0 mL). Then lithium hydroxide monohydrate (0.031 g, 3.0 eq.) was added and the reaction mixture was stirred overnight at RT. The crude mixture was concentrated in vacuo. The residue was purified on FCC (C18HP; H₂O:MeCN) to give the product (0.096 g, 76% yield) as white solid. ESI-MS: 514.4 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) 8.28 (d, J=5.0, 1H), 8.19 (dd, =8.1, 1.6 Hz, 1H), 8.01 (s, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.75-7.68 (m, 1H), 7.62 (d, =8.4 Hz, 1H), 7.42 (dd, J=9.0, 3.1 Hz, 1H), 7.40-7.34 (m, 1H), 7.27-7.20 (m, 2H), 6.67 (d, J=9.0 Hz, 1H), 4.78 (s, 1H), 4.21-4.11 (m, 2H), 3.85 (s, 3H), 3.80-3.54 (m, 7H), 3.43 (d, J=11.5 Hz, 1H), 2.81-2.61 (m, 2H), 2.37 (s, 3H), 2.03-1.91 (m, 1H), 1.83-1.70 (m, 1H), 1.57-1.37 (m, 2H).

Example 102. 5-[(3S)-3-{[(7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-7,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-yl]pyridine-2-carboxylic Acid

Preparation of Methyl 5-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-7-yl]pyridine-2-carboxylate

(S)-3-Boc-aminopiperidine (0.48 g, 1.3 eq.), methyl 5-bromopyridine-2-carboxylate (0.03 g, 1.0 eq.) and cesium carbonate (1.2 g, 2.0 eq.) were suspended in anh. 1,4-dioxane (5.0 mL). and the mixture was purged with argon for 10 min. Then Pd₂(dba)₃ (0.085 g, 0.05 eq.) and Xantphos (0.064 g, 0.06 eq.) were added. The reaction mixture was purged one more time with argon for 10 minutes and reaction was heated at 115° C. overnight. The mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. DCM was added and washed with water and brine. The organic layer was dried over anh. MgSO₄, filtered and concentrated in vacuo. The crude was purified by FCC (SiHP, DCM:MeOH), to give a product (0.206 g, 39% yield). ESI-MS: 336.5 [M+H]⁺.

Preparation of Methyl 5-[(3S)-3-aminopiperidin-1-yl]pyridine-2-carboxylate

4.0 M HCl in 1,4-dioxane (0.81 mL, 8.0 eq.) was added to solution of methyl 5-[(3S)-3-{[(tert-butoxy)carbonyl]amino}piperidin-1-yl]pyridine-2-carboxylate (0.157 g, 1.0 eq.) in 1,4-dioxane (4.0 mL). The reaction mixture was stirred at RT over the weekend. The solvent was evaporated under reduced pressure. Subsequently, the crude material was partitioned between NaOH aq and DCM. The organic layer was dried over anh. Na₂SO₄, filtered and concentrated to dryness to give product (0.085 g, 85% yield). ESI-MS: 236.2 [M+H]⁺.

Preparation of methyl 5-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylate

Methyl 5-[(3S)-3-aminopiperidin-1-yl]pyridine-2-carboxylate (0.080 g, 1.0 eq.), 2-methylpyridine-4-carbaldehyde (0.038 mL, 1.1 eq.) and sodium acetate (0.027 g, 1.0 eq.) were dissolved in anh. methanol (5.0 mL) and the reaction mixture was stirred at RT overnight. Then the mixture was cooled to 0° C. and sodium borohydride (0.014 g, 1.1 eq.) was added in portions and the reaction mixture was stirred at RT for 1 h. Methanol was evaporated. Product was purified by FCC (SiHP; DCM:MeOH) to give product (0.046 g, 38% yield). ESI-MS: 341.3 [M+H]⁺.

Preparation of methyl 5-[(3S)-3-{[(7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylate

7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carbaldehyde (Intermediate 9) (0.044 g, 1.1 eq.) and methyl 5-[(3S)-3-{[(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylate (0.046 g, 1.0 eq.) were dissolved in anh. DCE (3.0 mL). The resulting mixture was stirred at RT overnight. Then sodium triacetoxyborohydride (0.074 g, 2.8 eq.) was added and the mixture was stirred for another 24 h at RT. The reaction mixture was diluted with DCM, washed subsequently with sat. aq. sodium bicarbonate, water, brine, dried over anh. MgSO₄ and concentrated in vacuo. The residue was purified by FCC (C18HP; H₂O:MeCN) to give product (0.026 g, 36% yield). ¹H NMR (400 MHz, DMSO-d₆) δ 8.34 (d, J=3.0 Hz, 1H), 8.27 (d, J=5.0 Hz, 1H), 8.15 (d, J=6.2 Hz, 1H), 7.97 (d, J=9.5 Hz, 1H), 7.91 (s, 1H), 7.79 (d, J=8.9 Hz, 1H), 7.29 (dd, J=9.0, 3.0 Hz, 1H), 7.21 (s, 1H), 7.16 (d, J=5.4 Hz, 1H), 4.10 (d, J=12.8 Hz, 1H), 3.91 (d, J=13.1 Hz, 1H), 3.79 (s, 2H), 3.77 (d, J=2.8 Hz, 2H), 3.65 (s, 2H), 3.56-3.50 (m, 1H), 3.03 (d, J=12.0 Hz, 1H), 2.86 (t, J=12.4 Hz, 1H), 2.36 (s, 3H), 1.98 (d, J=12.3 Hz, 1H), 1.78 (d, J=13.3 Hz, 1H), 1.67 (q, J=11.3 Hz, 2H), 1.45 (d, J=13.1 Hz, 2H), 1.25-1.19 (m, 2H), 0.95-0.85 (qm, J2H).

Preparation of 5-[(3S)-3-{[(7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylic Acid

Lithium hydroxide monohydrate (0.003 g, 4.5 eq.) was added to a solution of methyl 5-[(3S)-3-{[(7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylate (0.018 g, 1.0 eq.) in THF (3.0 mL) and water (1.0 mL). The resulting mixture was stirred at RT overnight. The reaction mixture was diluted with DCM and washed with conc. aq. HCl. The crude was purified by FCC (C18HP, H₂O:MeCN) to give product (0.005 g, 31% yield). ESI-MS: 576.6 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.27 (s, 1H), 8.16 (t, J=6.3 Hz, 2H), 8.01 (d, J=9.5 Hz, 1H), 7.97 (s, 1H), 7.94 (d, J=8.9 Hz, 1H), 7.44 (dd, J=8.7, 2.4 Hz, 1H), 7.26 (s, 1H), 7.22 (d, J=5.3 Hz, 1H), 4.16 (d, J=11.9 Hz, 1H), 3.92 (s, 1H), 3.86 (s, 2H), 3.79 (s, 2H), 3.48 (quint, J=7.3, 3.4 Hz, 1H), 3.10 (t, J=12.1 Hz, 1H), 2.97-2.87 (m, 2H), 2.35 (s, 3H), 2.15 (d, J=11.8 Hz, 1H), 1.93 (d, J=12.8 Hz, 1H), 1.82-1.71 (m, 1H), 1.70-1.61 (m, 1H), 1.33-1.27 (m, 2H), 0.94 (dt, J=6.2, 3.4 Hz, 2H).

Example 103. 5-1(3S)-3-{[(7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-ylpyridine-2-carboxamide

Preparation of 5-1(3S)-3-{[(7-Chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-ylpyridine-2-carboxamide

A mixture of methyl 5-[(3S)-3-{[(7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinolin-3-yl)methyl][(2-methylpyridin-4-yl)methyl]amino}piperidin-1-yl]pyridine-2-carboxylate (0.029 g, 1.0 eq.) in 7.0 N ammonia solution in MeOH (5.0 mL) was stirred at RT overnight. Then the mixture was heated to 50° C. for 1.5 h. The solvent was evaporated and the crude material was purified by FCC (C18HP, H₂O:MeCN) to give the product (0.021 g, 84% yield). ESI-MS: 575.7 [M+H]⁺. ¹H NMR (400 MHz, Methanol-d₄) δ 8.26 (d, J=2.9 Hz, 1H), 8.17 (dd, J=8.8, 5.7 Hz, 2H), 8.02 (d, J=9.4 Hz, 1H), 7.98 (s, 1H), 7.88 (d, J=8.8 Hz, 1H), 7.35 (dd, J=8.9, 2.9 Hz, 1H), 7.26 (s, 1H), 7.22 (d, J=5.1 Hz, 1H), 4.13 (d, J=12.3 Hz, 1H), 3.89 (s, 1H), 3.86 (d, J=2.7 Hz, 2H), 3.80 (s, 2H), 3.52-3.45 (m, 1H), 3.06 (t, J=12.1 Hz, 1H), 2.95-2.85 (m, 2H) J, 2.36 (s, 3H), 2.15 (d, J=11.9 Hz, 1H), 1.92 (d, J=12.2 Hz, 1H), 1.81-1.71 (m, 1H), 1.70-1.59 (m, 1H) J, 1.34-1.27 (m, 2H), 0.99-0.90 (m, 2H).

Biological Assays and Data:

As stated above, the compounds of the present invention are STING modulators and are useful in treating diseases by STING activity regulation. The biological activity of the compounds of the present invention can be determined by any appropriate test to determine the activity of the compound as STING modulator, as well as cell lines and in vivo models.

Fluorescence Thermal Shift Assay

Compounds of the present invention were tested for binding to human STING in Fluorescence Thermal Shift assay. STING was preincubated with the compounds for 20 minutes in 50 mM Hepes, 150 mM NaCl, pH 7.5 in 15 or 16 μl volume, following by adding 4 μl of SyproOrange dye dilution (ThermoFisher, cat no. S-6651). Final STING concentration was 0.1 mg/mL. Thermal unfolding was performed in Real-Time PCR QuantStudio 6 Flex System (Applied Biosystems), from 25 to 99° C., with continuous ramp mode and ramp rate 0.033° C./s. The data were analyzed using Protein Thermal Shift Software (ThermoFisher).

Using the Fluorescence Thermal Shift assay described above, Examples 1-35, 37, 45-47, 49, 55-58, 66-68, 75, 78, 82, 85, 86, 95, 98, 99 exhibited ΔTm [° C.] values in the following ranges: +=ΔTm≤10° C.; ++=10<ΔTm<20° C.; +++=ΔTm≥20° C. For following examples ΔTm [° C.] of FTS assay are:

hSTING FTS binding assay Examples (ΔT ° C.) 1 + 2 +++ 3 + 4 +++ 5 +++ 6 ++ 7 +++ 8 +++ 9 +++ 10 +++ 11 +++ 12 +++ 13 +++ 14 +++ 15 +++ 16 +++ 17 +++ 18 + 19 +++ 20 +++ 21 +++ 22 +++ 23 ++ 24 +++ 25 +++ 26 +++ 27 +++ 28 +++ 29 +++ 30 +++ 31 +++ 32 +++ 33 +++ 34 +++ 35 +++ 37 +++ 45 +++ 46 ++ 47 +++ 49 + 55 +++ 56 +++ 57 +++ 58 +++ 66 +++ 67 +++ 68 +++ 75 +++ 78 +++ 82 ++ 85 ++ 86 +++

THP-1 Dual Reporter Assay

Compounds of the present invention were tested for their activity using THP-1 dual cells (Invivogen, cat no. thpd-nfis) allowing for simultaneous study of NF-κB pathway and the interferon regulatory factor (IRF) pathway. THP-1 dual cells contain luciferase reporter gene under the control of an ISG54 (interferon-stimulated gene) minimal promoter in conjunction with five interferon-stimulated response elements and a secreted embryonic alkaline phosphatase reporter gene under the control an IFN (interferon)-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element with three copies of the c-Rel binding site. Following 18 h of stimulation with STING agonist, medium was collected and transferred onto fresh cell culture plate. To verify activity of the IRF pathway, luminescence activity was measured with standard laboratory plate reader immediately after addition 10 μl of the medium to 40 or 50 μl of luminescence reagent (Invivogen, cat. No. rep-qlc2). To verify activity of NF-κB pathway, 10 or 20 μl of the medium was mixed with 80 μl of a detection medium (Invivogen, cat. No. rep-qb2) and incubated for 1 or 2 hours at 37° C. in a humidified atmosphere containing 5% CO₂. Next, absorbance at 630 or 655 nm was recorded using standard laboratory plate reader.

Compounds of the present disclosure, as exemplified in Examples 1-103, showed EC50 values in the following ranges: +=EC₅₀≥10 μM; ++=1 μM<EC₅₀<10 μM; +++=EC₅₀≤1 μM.

THP1 THP1 Dual Dual EC50 EC50 (μM) (μM) Examples Structure IRF NFkB  1

++ ++  2

+++ +++  3

+++ ++  4

+++ +++  5

+++ +++  6

++ ++  7

+++ +++  8

+++ +++  9

+++ +++ 10

+++ +++ 11

+++ +++ 12

+++ +++ 13

+++ +++ 14

+++ +++ 15

+++ +++ 16

+++ +++ 17

+++ +++ 18

++ ++ 19

+++ +++ 20

+++ +++ 21

+++ +++ 22

+++ +++ 23

+++ +++ 24

+++ +++ 25

+++ +++ 26

+++ +++ 27

+++ +++ 28

+++ +++ 29

+++ +++ 30

+++ +++ 31

+++ +++ 32

+++ +++ 33

+++ +++ 34

+++ +++ 35

+++ +++ 36

+++ +++ 37

+++ +++ 38

+++ +++ 39

+++ +++ 40

++ ++ 41

++ ++ 42

+++ +++ 43

++ ++ 44

+ + 45

+++ +++ 46

+ + 47

+++ +++ 48

+++ ++ 49

+ + 50

+++ +++ 51

+++ ++ 52

+++ +++ 53

+++ +++ 54

+++ ++ 55

+++ +++ 56

+++ +++ 57

+++ +++ 58

+++ +++ 59

+ + 60

+++ +++ 61

+++ +++ 62

+++ +++ 63

+++ +++ 64

+++ +++ 65

+++ +++ 66

++ ++ 67

++ ++ 68

+++ +++ 69

++ ++ 70

+++ +++ 71

+++ +++ 72

+++ +++ 73

+++ +++ 74

++ ++ 75

+++ +++ 76

++ ++ 77

++ ++ 78

+++ +++ 79

+++ +++ 80

+++ +++ 81

+++ +++ 82

+++ +++ 83

+++ +++ 84

+++ +++ 85

+++ +++ 86

+++ +++ 87

+++ +++ 88

+++ +++ 89

+++ +++ 90

+++ +++ 91

+++ +++ 92

+++ +++ 93

+++ +++ 94

+++ +++ 95

+++ +++ 96

++ + 97

+++ +++ 98

+++ +++ 99

+++ +++ 100

+++ +++ 101

++ ++ 102

++ ++ 103

+++ +++

In Vivo Anti-Tumor Efficacy

The efficacy of Example 4, Example 31 and Example 57 was evaluated in established CT26 murine colon carcinoma allografts in female Balb/C mice. Compounds were formulated in 5% ethanol with 10% Captisol in PBS and administered intravenously, once every fifth day on three occasions. The solution was prepared fresh prior to each administration. CT26 tumors responded prominently to the treatment with Example 4, Example 31 and Example 57. Both dose levels of Example 4, 7 and 10 mg/kg, were efficacious and caused tumor growth delay or significant decrease of the tumor size compared to the control group or day volume at randomization, respectively (FIG. 1A-FIG. 1C). By the end of the study there was one complete response among animals treated at 10 mg/kg of Example 4. Treatment with Example 31 resulted in slight tumor growth delay when treated at 7 mg/kg when compared to the control group (FIG. 2A-FIG. 2C). Both 15 mg/kg and 30 mg/kg of Example 31 were prominently efficacious, and treatment with the higher dose resulted in two complete responses by the end of the study on day 43. CT26 tumors responded to treatment with Example 57 in a dose dependent manner with moderately efficacious dose at 10 mg/kg and full efficacious dose at 20 mg/kg (FIG. 3A-FIG. 3C). In the group treated at 5 mg/kg only a minimal tumor growth delay was observed when compared to the control group. By the end of the study, on day 43, there was one complete response recorded in group treated at 20 mg/kg of Example 57. 

1. A compound of formula (I)

or a salt, stereoisomer, tautomer, or N-oxide thereof, wherein X¹ is CH or N; X² is CR³ or N; R¹, R² and R³ are independently H, OH, NR^(C)R^(D), CN, halogen, C₁-C₄-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₄-alkoxy, aryloxy, benzyloxy, C(═O)R^(E), NR^(F)C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D), or 4- to 6-membered saturated, partially or fully unsaturated, or aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl, heterocyclyloxy, or heterocyclyl-C₁-C₂-alkyl, or 8- to 10-membered saturated, partially or fully unsaturated, or aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned heterocyclic or heterobicyclic rings comprise one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X); R⁴ is a 5- or 6-membered aromatic carbocyclic or heterocyclic ring, or a 9- or 10-membered aromatic carbobicyclic or heterobicyclic ring, wherein the heterocyclic or heterobicyclic ring comprises at least one nitrogen atom and optionally one or more, same or different additional heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned cyclic rings is independently unsubstituted or substituted with one or more same or different substituents R^(X); R⁵ is a 5- or 6-membered saturated heterocyclic ring, wherein said heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom is independently unsubstituted or substituted with one or more, same or different substituents R^(Y); and wherein R^(N) is H, C₁-C₄ alkyl, HO(C═O)—C₁-C₄-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, C₁-C₂-alkoxy-C₁-C₄-alkyl, or a 3- or 4-membered saturated carbocyclyl or heterocyclyl, wherein said heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X); R^(C) and R^(D) are independently H, or C₁-C₂-alkyl; or R^(C) and R^(D) together with the nitrogen atom to which they are bonded form a 5- or 6-membered saturated, partially or fully unsaturated, or aromatic heterocyclic ring, wherein said heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the heterocyclic ring is independently unsubstituted or substituted with one or more, same or different substituents R^(X); R^(E) is H, C₁-C₂-alkyl, NR^(C)R^(D)—C₁-C₄-alkyl, phenyl, benzyl, OR^(G), or NR^(H)R^(I); or a 5- or 6-membered saturated, partially or fully unsaturated heterocyclyl, wherein said heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents RE; R^(E) is H, C₁-C₂-alkyl, C₃-C₆-cycloalkyl, phenyl, benzyl, or C(═O)NR^(H)R^(I); R^(G) is H, C₁-C₂-alkyl, or 5- or 6-membered aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl, or heterocyclyl-C₁-C₂-alkyl, wherein the aforementioned heterocyclic rings comprise one or more, same or different heteroatoms selected from O, N or S, wherein said N-atoms are independently oxidized or non-oxidized; R^(H) and R^(I) are independently H, C₁-C₂-alkyl, or 5- or 6-membered aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl, or heterocyclyl-C₁-C₂-alkyl, wherein the aforementioned heterocyclic rings comprise one or more, same or different heteroatoms selected from O, N or S, wherein said N-atoms are independently oxidized or non-oxidized; or R^(H) and R^(I) together with the nitrogen atom to which they are bonded form a 5- or 6-membered saturated, partially or fully unsaturated, or aromatic heterocyclic ring, wherein said heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the heterocyclic ring is independently unsubstituted or substituted with one or more, same or different substituents R^(X); R^(X) is OH, NR^(C)R^(D), halogen, CN, NO₂, C₁-C₂-alkyl, C₁-C₂-haloalkyl, NR^(C)R^(D)—C₁-C₄-alkyl, R^(C)O—C₁-C₄-alkyl, C₁-C₂-alkoxy, C(═O)R^(E), or two R^(X) form ═O, or two R^(X) together with the carbon atom to which they are bonded form a 3- to 5-membered saturated, partially or fully unsaturated, or aromatic carbocyclic ring; R^(Y) is halogen, CN, OH, C₁-C₂-alkyl, HO—C₁-C₂-alkyl, C₃-C₆-cycloalkyl, C₁-C₂-alkoxy, NR^(C)R^(D), S(═O)₂NR^(C)R^(D), C(═O)R^(E), or 5- or 6-membered saturated, partially or fully unsaturated, or aromatic carbocyclyl, carbocyclyl-C₁-C₂-alkyl, heterocyclyl, and heterocyclyl-C₁-C₂-alkyl, wherein the aforementioned heterocyclic rings comprise one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X); or two RY form ═O; or two RY attached to identical or neighboring carbon atoms may form a 3-membered carbocyclic ring; with the proviso that either any one of R¹, R², or R³ is NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D), or 8- to 10-membered saturated, partially or fully unsaturated, or aromatic carbobicyclyl or heterobicyclyl, wherein the aforementioned heterobicyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X); or any one of R¹, R², or R³ is NR^(F)C(═O)R^(E), wherein R^(E) is NR^(C)R^(D)—C₁-C₄-alkyl; or R^(N) is NR^(C)R^(D)—C₁-C₄-alkyl, or C₁-C₂-alkoxy-C₁-C₄-alkyl; or any one of the substituents R¹, R², R³, R⁴, R⁵, R^(N) carries a substituent R^(X), wherein R^(X) is OH, NR^(C)R^(D), NR^(C)R^(D)—C₁-C₄-alkyl, or R^(C)O—C₁-C₄-alkyl.
 2. The compound according to claim 1, with the proviso that either R² is NHR^(D), NHR^(D)—C₁-C₄-alkyl, NH—(C₁-C₄-alkylene)-NR^(C)R^(D), NR^(F)—(C₁-C₄-alkylene)-NHR^(D), O—(C₁-C₄-alkylene)-NHR^(D), or 8- to 10-membered saturated heterobicyclyl, wherein the aforementioned heterobicyclic ring comprises at least one nitrogen atom and optionally one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or 5-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X); or R² is NR^(F)C(═O)R^(E), wherein R^(E) is NHR^(D)—C₁-C₄-alkyl; or R^(N) is NHR^(D)—C₁-C₄-alkyl; or any one of the substituents R², R⁴, R⁵, R^(N) carries a substituent R^(X), wherein R^(X) is OH, NHR^(D), NHR^(D)—C₁-C₄-alkyl, or HO—C₁-C₄-alkyl.
 3. The compound according to claim 1 or 2, with the proviso that either R² is NH₂, NH₂—C₁-C₂-alkyl, NH—(C₁-C₃-alkylene)-NH₂, NH—(C₁-C₃-alkylene)-NHCH₃, NH—(C₁-C₃-alkylene)-N(CH₃)₂, O—(C₁-C₃-alkylene)-NH₂, or O—(C1-C₃-alkylene)-NHCH₃; or R² is NHC(═O)R^(E), wherein R^(E) is NH₂—C₁-C₄-alkyl; or R^(N) is NH₂—C₁-C₃-alkyl; or any one of the substituents R², R⁴, or R⁵ carries a substituent R^(X), wherein R^(X) is OH, NH₂, NHCH₃, NH₂—C₁-C₂-alkyl, or HO—C₁-C₂-alkyl.
 4. The compound according to any one of claims 1 to 3, wherein X¹ is CH; and X² is CR³ with R³ being H.
 5. The compound according to any one of claims 1 to 4, wherein R¹ is H or F.
 6. The compound according to any one of claims 1 to 5, wherein R² is H, OH, NR^(C)R^(D), CN, halogen, NR^(C)R^(D)—C₁-C₄-alkyl, NR^(F)C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-C(═O)R^(E), NR^(F)—(C₁-C₄-alkylene)-NR^(C)R^(D), O—(C₁-C₄-alkylene)-NR^(C)R^(D), or 4- to 6-membered saturated heterocyclyl, or 8- to 10-membered saturated heterobicyclyl, wherein the aforementioned heterocyclic or heterobicyclic rings comprise a nitrogen atom and optionally one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X).
 7. The compound according to any one of claims 1 to 6, wherein R^(N) is H, C₁-C₄-alkyl, HO(C═O)—C₁-C₃-alkyl, NHR^(D)—C₁-C₃-alkyl, C₁-C₂-alkoxy-C₁-C₃-alkyl, or cyclopropyl.
 8. The compound according to any one of claims 1 to 7, wherein R⁴ is pyridinyl, wherein each substitutable carbon atom in the cyclic ring is independently unsubstituted or substituted by one or more, same or different substituents R^(X).
 9. The compound according to any one of claims 1 to 8, wherein R⁵ is piperidine, wherein each substitutable carbon or heteroatom in the piperidine ring is independently unsubstituted or substituted by one or more, same or different substituents R^(Y).
 10. The compound according to any one of claims 1 to 9, wherein R^(C) is H or C₁-C₂-alkyl; R^(D) is H or C₁-C₂-alkyl; R^(E) is NH₂—C₁-C₄-alkyl; R^(E) is H; R^(X) is halogen, OH, NH₂, NHCH₃, C₁-C₂-alkyl, C₁-C₂-alkoxy, NH₂—C₁-C₂-alkyl, HO—C₁-C₂-alkyl, or two R^(X) together with the carbon atom to which they are bonded form a 3-membered saturated carbocyclic ring; R^(Y) is halogen, OH, NH₂, or a 5- or 6-membered aromatic heterocyclyl, wherein the aforementioned heterocyclic ring comprises one or more, same or different heteroatoms selected from O, N or S, wherein said N- and/or S-atoms are independently oxidized or non-oxidized, and wherein each substitutable carbon or heteroatom in the aforementioned groups is independently unsubstituted or substituted with one or more, same or different substituents R^(X).
 11. The compound according to any one of claims 1 to 10, wherein the compound according to formula (I) is selected from the group consisting of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; 7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one.
 12. The compound according to any one of claims 1 to 10, wherein the compound according to formula (I) is selected from the group consisting of 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-methyl-1,4-dihydroquinolin-4-one; 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methoxypyridin-4-yl)methyl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; 7-[(3R)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3S)-3-aminopyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3S)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3R)-3-aminopiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 3-({[(2-aminopyridin-4-yl)methyl][(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl]amino}methyl)-1-cyclopropyl-6,7-difluoro-1,4-dihydroquinolin-4-one; 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-7-{[2-(dimethylamino)ethyl]amino}-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-{7-amino-5-azaspiro[2.4]heptan-5-yl}-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-6,7-difluoro-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; 7-(4-amino-3,3-difluoropiperidin-1-yl)-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)azetidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-bromo-1-methyl-1,4-dihydroquinolin-4-one; 7-[3-(aminomethyl)-3-fluoropyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-(4-hydroxypiperidin-1-yl)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 3-({[(3S)-1-(6-aminopyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; 7-amino-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydro-1,8-naphthyridin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydro-1,8-naphthyridin-4-one; 1-cyclopropyl-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1-(propan-2-yl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(4R)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 7-[(4S)-4-amino-3,3-difluoropiperidin-1-yl]-1-cyclopropyl-6-fluoro-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-(methylamino)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[1-(2-hydroxyethyl)-1H-pyrazol-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methoxypyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(2S)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(2R)-2-(hydroxymethyl)morpholin-4-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methyl pyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-hydroxypyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(3S)-1-(6-methylpyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(3S)-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(5-methylpyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-[(3S)-3-hydroxypiperidin-1-yl]-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-(2-hydroxyethoxy)-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrol id in-1-yl]-3-({[(2-methyl pyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyridin-3-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyridin-3-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3R)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-3-({[(3S,5S)-5-fluoro-1-(pyrazin-2-yl)piperidin-3-yl][(2-methylpyridin-4-yl)methyl]amino}methyl)-7-(2-hydroxyethoxy)-1,4-dihydroquinolin-4-one; 1-cyclopropyl-6-fluoro-7-[(3S)-3-hydroxypiperidin-1-yl]-3-({[(2-methylpyridin-4-yl)methyl][(3S)-1-(pyrazin-2-yl)piperidin-3-yl]amino}methyl)-1,4-dihydroquinolin-4-one.
 13. A pharmaceutical composition comprising a pharmaceutically effective amount of the compound according to any one of claims 1 to 12 and optionally a pharmaceutically acceptable carrier, diluent or excipient.
 14. A compound according to any one of claims 1 to 12 or a pharmaceutical composition according to claim 13 for use in medicine.
 15. A compound according to any one of claims 1 to 12 or a pharmaceutical compositions according to claim 13 for use in the treatment of a disease selected from the group consisting of cancer, pre-cancerous syndromes, and infectious diseases; or for use in an immunogenic composition or as vaccine adjuvant.
 16. A compound according to any one of claims 1 to 12 or a pharmaceutical composition according to claim 13 for use in the treatment of a disease selected from the group consisting of inflammatory diseases, allergic diseases, and autoimmune diseases. 